Science topic
Tribolium - Science topic
A genus of small beetles of the family Tenebrionidae; T. confusum is the "confused flour beetle".
Questions related to Tribolium
Hi,
I plan to work on crickets (orthopterans) and Red flour beetle (Tribolium castaneum). Can anyone suggest to me a reference that these organisms do not need Bio-safety clearance or can be dealt with as BSL-1?
I am from India.
Many thanks.
Rittik
Currently, in order to identify insect eggs in stored or processed products (such as contaminated flour or wafer samples), staining was performed based on the method mentioned in the below article.
Microscopic images of possible identified cases were taken with a magnification of 10*20 X, as follows.
Please guide us about the accuracy of detected eggs from other residues (flour) as well as the type of species (beetles) related to them?
Can this method be also used for the detection of larvae and pupa?
And
In general, what is the fastest way to detect and identify each stages of beetle life cycle (egg, larvae and pupa) in stored or processed products?
15 non-injected Tribolium casteneum 6th Instar larvae kept at 28oC for 10 days in 0.5g of flour, 95% organic wholemeal flour and 5% bakers yeast, in each replicate.
Larvae survival is recorded daily. The flour containing the larvae is poured onto a piece of paper. Larvae are separated from the flour using a small paintbrush to help count the larvae. once survival is recorded the larvae are brushed into the container, followed by the flour.
I believe that this brushing into their container daily may be responsible for the unexpected deaths in my control.
I would be very appreciative if anybody could help provide any supporting material for this belief? or any alternative reasons?
Does anyone have, or know of a lab that has, a colony of Tribolium beetles infected with the ectoparasitic mite Acarophenax lacunatus or A. tribolii? Biological supply houses (e.g. Carolina) only have clean colonies. I was hoping to set up some simple experiments for a parasitology class I teach. Thanks in advance.
Doug Graham
As a part of molecular identification of Tribolium in food products, what is the most suitable method to isolate DNA from Tribolium sp.?
Primers for Tribolium and Sitophilus population screening.
Galanthamine hydrobromide is a good compound for positive control, but it is very expensive, which other compounds can be used as positive assay against the flour beetle Tribolium castaneum?
in many papers DEET is used as repellent, could be used also as insecticide?
please provide me a reference
My aim is to identify phenotypic variation in T. castaneum. Therefor i will take fotos and want to measure for example quantitative traits. Like the eye size and antennae length in larvae.
I am looking for a source of the flightless phenotype Tribolium castaneum. I will be using a shared laboratory space for toxicological studies and want to ensure containment. Thanks, Lou
