Thyroid Hormones - Science topic

Hi,

I have mullet larvae samples preserved in RNA save.

Can I perform immunofluorescence for thyroid hormones on these samples?

Do you think it will work?

In many papers, performing thyroid hormone extraction using barbital.

there are a few different compounds of barbital in the market (Sodium barbiturate-C4H3N2NaO3, Sodium 5,5-diethylbarbiturate-C8H11N2NaO3, Barbital-C8H12N2O3).

Which compound is the best for extracting TH from whole fish larvae?

Thx.

disorder means irregular level of thyroid hormone T3,T4,TSH

pregnancy diagnosis in mare

Since the paper of Weiss et al 2009 and Hamers et al 2020 it is well described that PFAS are disrupting the thyroid hormon transport TTR. So far as published only a handfull PFASs has been measured for this important in vitro toxicity endpoint. Are their other published data for in vitro thyroid hormone disruption such as ToxCast or EU ChemScreen or Norman network data based?

How would expression of a gene targeted by thyroid hormone be affected by deletion of thyroid hormone receptor in the absence of hormone? How about in thyroid hormone-treated cells? Please, elaborate your answers.

Radioactive iodine causes goiter loss or shrinkage of toxic nodes that produce thyroid hormone.

Does garlic have an effect on this hormone and decrease or increase them?

I am a third year graduate student in a lab that focuses on growth hormone. My project, however, is extremely thyroid hormone specific. From most of the literature I have found, it seems thyroid hormones are measured by RIA instead of ELISA. This leads me to two questions:

Is there a specific reason RIA is used over ELISA?

Can an ELISA be used to measure serum T4 and T3 levels in mice effectively?

Thanks!

I am doing a correlation study of thyroid hormones (t3 and t4) in serum and urine, using immunoassay, I need some help on how to prepare urine, and what could be the best method to concentrate the hormones in urine to draw stronger correlation?( urine Collected is spot urine)

I am now having a human serum with the mixture of T3 and T4 with T4 concentration is 100 times bigger than T3 concentration.

The anti-T3 antibody that I already had does not have high specificity, so I got interfered by T3 with results in bad results.

Do you know which method to separate or remove T4 in a human serum before detecting T3?

Thank you.

As I read in papers, before doing the radioimmunoassay (RIA) to detect thyroid hormones such as T3, we should free the hormones from binding the carrier proteins like thyroxine-binding protein (TBG) and albumin for higher accuracy. And 8-Anilino-1-naphthalenesulfonic acid (ANS) is one of the most widely used chemical reagents that can do that liberating purpose.

I searched several papers, however, they did not describe how ANS could do that such function?

Is it like a competitve substance that goes into protein and occupies T3 binding site? Or it will change the structure of the protein? Or something else...

If anyone has the explanation or documents/papers/books related to this issue, please share to me.

Thank you very much.

What is the effect of thyroid hormones on dietary metabolism?

I am looking for ELISA kits with the best sensitivity to measure fasting plasma insulin and leptin in newborn rats.

In a study, we investigated TSH, T4 as well as T3 were increased together!

Do you know which interference has occurred? and in what stage?

We treated male rats in Oxymorphone drug and then serum levels of thyroid hormones were evaluated.

I appreciate for your kind response.

With regards,

Thyroxine treatment in hypothyroidism.

Thyroid Hormones, HbA1C

how work thyroid hormon ?

My group has observed that more than 70% require thyroid hormone to improve their insulin resistance indices

We hypothesize that diabetes would be a consequence of a damaged thyroid.

It would be interesting to collaborate on the topic of Diabetes and Thyroid Disease

I want to conduct an experiment for determining thyroid gland function. I came to know that both RIA and ELISA technique is used for this purpose among which ELISA is the economic one.

Every ELISA I have come across is usable for plasma/ serum, but not specifically for culture effluent. Can anyone make a suggestion for how to measure the hormone production?

Regards

Andy.

I'm looking for a commercially available thyroid-deplete fetal calf serum for cell culture work with mouse and human MSCs.

The products suggested on the previous question asking this (https://www.researchgate.net/post/Thyroid_hormone-free_serum) are no longer available and the only products I can find are human, which I am keen to avoid due the significant variation between batches.

With the goals of reducing sample volume and sample stability, I am interested in any information or a review of the use (methods) of blood spots for later analysis of TSH, bound and free T3 and T4. How do results from blood spot analysis compare with traditional plasma EIA?

Thanks!

I have conducted toxic studies in rodents up to three generations, wherein ANOVA followed by two posthoc tests (DMRT and Bonferroni) was used to compare control group with the toxic treated group. Kindly suggest me with which would be the best posthoc test, DMRT or Bonferroni to be conducted ?

Thyroid

I want to study the effect of  hypothyroidism on parts of brain?

I need to know the levels of t3,t4 and TSH in serum of hypothyroid mice?

relation exists

Thyroid profile

I'm working on a T3/rT3 SPE extraction using an anion exchange SPE column. I have stumbled across DCM as a wash step in the literature, but if there is a non-halogenated alternative I'm not aware of, I could sure use the ideas. Thanks in advance!

Since all researches available to me said that creatine monohydrate has no significant effect on triglycerides or T3 and one of these researches showed decreases in T3 and increases in T4, where the authors suggested the causes stands behind that results , yet I didn’t find a potential mechanism responsible for the increase of triglycerides and T3 in sera of broiler chicks at 42nd day and if there is an explain please just attach the references related to that.

I would like to undertake a thyroid treatment and monitoring assay in Xenopus tropicalis. I am new to using this is a s a model organism and would like to know if there are any classical landmark studies that have been undertaken in Xenopus sps. so I can get some background. Thank you.

Hi all,

I'm thinking about less invasive ways to measure thyroid hormone concentration in humans. Is it reasonable to consider hair and/or urine for this purpose? 

Thank you in advance!

I am planning to treat a rodent species with thyroid hormones (T4 and T3) for 4 weeks using Alzet osmotic pumps (Model 2006). Does anyone have an established protocol or can give me some advice regarding the appropriate solvent (e.g. propylen glycol?) and the stability of the hormones in the pump during implantation. Regards and thanks in advance, yoshi.

I need the fast model to induced that.

What is the normal value for T3, T4, and TSH in Wistar Rat?

I am studying a mouse model with reduced whole body development and I would need to evaluate if there is any alteration in the thyroid function. Some publications employed TSH levels, while others T3 or T4. Which one is more accurate?

I found in an immunization scheme that a mouse had large thyroid. This scheme was with T4-BSA as immunogen.

What is the method for synthesis of thyroxine after 2010?

Liothyronine.

In clinical practice, frequently supplementation with synthetic or natural thryroid hormone is used. This inevitably results in a low TSH because of feedback inhibition by triiodothyronine (T3) on synthesis and release of T4 from the thyroid gland. Endocrinologists often express a concern that "the TSH is too low", ie below the accepted clinical range. None have been able to explain to me what are the long term consequences of having a low TSH in and of itself. What is too low? Since it must be expected in thyroid supplementation, I fail to see the concern, and I cannot find relevant publications that would explain to me the ramification of low TSH. I call on the world-wide expertise of ResearchGate participants.

I am looking for bovine serum that has been depleted of thyroid hormone (both T3 and T4). Does anyone know of a commercial source or a straightforward way to strip bovine serum of thyroid hormone in house?

I would like to measure the concentration of T3 and T4 in zebrafish, and I'm trying to figure out the best way to extract the hormones from fish tissues. I tried extracting in phosphate buffer, but the concentrations were too low. Most of the protocols I find use barbital buffer, which I would like to avoid if at all possible, since it is a controlled substance and requires DEA certification. Any assistance would be greatly appreciated!