Moringa oleifera - Science topic

While Guar Gum can act as a flocculant for dyes, its lack of charge makes it ineffective as a flocculant for most freshwater microalgae. It appears to only be viable if it is modified adding a cationic moeity in its backbone.

Aditionally filamentous fungi effect on flocculation may vary in its effect depending on the microalgae. There are more examples of this fungi in the literature but most are from the Aspergillus genus.

Overall they seem to be food grade but from my own research the direct effects on humans havent been tested for most of them.

Some aditional bioflocculants you could add are: cationic polyssacharydes such as cationic starch ( ), guar gum (http://dx.doi.org/10.1016/j.carbpol.2012.09.022) and

Have a look at the following RG links.

I think this document could be useful:

I guess your intention is to get as much quercetin, chlorogenic acid and tocopherols as possible. For that purpose, and using ethanol as solvent, I would choose a percolation method. I will not choose soxhlet, cause the solvent will reach ~78ºC and will ruin the extract. I would use the simple percolation method (https://pharmacyscope.com/percolation-extraction/) using cold ethanol. I personally would use 40-45 ºC ethanol heated in a water bath in a fume hood, but, that procedure has some associated risks due ethanol vapors and I wouldn't recommend it.

Thanks very much Hamid Reza Farhang Ikram Madani Ahmed Kashif Ameer

Dear Lukas Behr,

Firstly, Spirulina (Arthrospira platensis) is a filamentous and multicellular blue-green alga. Eventually, it doesn't require too much effort for harvesting.

Secondly, using chemical flocculants for harvesting microalgae is not advisable for food/pharmaceutical applications because the chemicals might have toxic effects on the final products. On the other hand, physical harvesting, for instance, moving net, could be promising.

Please check the video link below:

Thank you

Best of luck

Chandan

Please see this file

Moringa oleifera boost the immune system of the body thus providing protection against SARS-CoV-2 infection.

When we use any chemical, we need to clearly understand the expiry date of this substance. The validity period not only means a change in its chemical or biological structure, but also a change in its physical state. In your case, NaCl will be further used for salt extraction of moringa oleifera seeds. Although its chemical structure is relatively stable, the physical state of NaCl is likely to change during long-term storage and use (such as water absorption, etc.). This series of small deviations may affect your experimental results. I recommend that if you want to accurately achieve your desired goal, you should choose a new chemical instead of an expired one.

Dear Dr. Crhistian Mark Montenegro

What is the problem if you have got regenerated plants from leaves via tissue culture, are you worried about the media components, although normal plants grown in fields have to be provided with macro- and micro-elements to grow.

Because Sunflower meal is an expensive protein source and not easily available in all over the world , that's why we are trying to use those proteins source ingredients which are easily available and benefits of farmers

Dear Mohd. Arif thank you for this interesting technical question. Just in case that this is still of interest to you, please have a look at the following potentially useful review article (book chapter):

(see attached pdf file)

Dear Shriyedita Ramesh thank you for this very interesting and important technical question. In this context please have a look at the following relevant article:

This paper is freely available as public full ext on ResearchGate.

Also please see this potentially useful article:

Also check https://pubmed.ncbi.nlm.nih.gov/23905019/

Actually, the article does not contain the process @Bayan Hussien

Yes you are right. Some of the compounds are very difficult to separate. Semi-Preparaive HPLC will help. That was what I used.

Agricultural Research Council - Agricultural Engineering campus (Pretoria)

Tel: 012 842 4017

Yes! Please take a look at this RG link.

Several studies on Moringa oleifera reports anti-fertility and abortion activities of its leaves, roots and stems. Please see the following RG links and PDF attachments.

Moringa oleifera is known for its many benefits and medicinal properties, as it is beneficial for hair and skin, anti-inflammatory, reduces the risk of cancer, is also beneficial for digestive system and colon, strengthens bones as it contains calcium and phosphorus, maintains cardiovascular health and blood pressure, reduces the level of fat and cholesterol in the blood, lowers blood sugar level, Helps heal wounds, helps alleviate asthma, helps iron absorption and thus prevents anemia.

Shantanu Bhunia, i was asking a method for leave proteins isolation, not how to determined protéines content. Thank you

@ Lily Oguh-Olayinka

Please also have a look at the following RG links.

Thanks!

Does anybody have the surface morphology of Advera material with some explaination.Many thanks

Dear it is because some of them are lectins that agglutinate some blood.

Hi ,

You can check the following link as well

Transcriptome of specific tissues is also available.

Hope this helps.

Regards

Pankaj

Dear Khawla Al-saidi,

Moringa oleifera seeds treat water on two levels, acting both as

01. Coagulant &

02. Antimicrobial agent.

Moringa works as a coagulant due to positively charged, water-soluble proteins, which bind with negatively charged particles (silt, clay, bacteria, toxins, etc) allowing the resulting “flocs” to settle to the bottom or be removed by filtration.

The antimicrobial aspects of Moringa continue to be researched.

Findings support recombinant proteins both removing microorganisms by coagulation as well as acting directly as growth inhibitors of the microorganisms.

While there is ongoing research being conducted on the nature and characteristics of these components, it is accepted that treatments with Moringa solutions will remove 90-99.9% of the impurities in water.

I hope I have answered your question.

With Best Wishes,

Samir G. Pandya

Hello Maliha!

Possible reasons of fungal contamination in MS media could be-

1. Improperly sterilized media and/equipments.

2. Defect of air filter of laminar flow.

3. Movement of peoples behind you when you worked under hood.

4. No mask on your mouth while you inoculated.

Please double check the above mentioned possible reasons of contamination.

Dear Sir. Concerning your issue about the best LC-MS/MS analysis of phenolic compound from Moringa oleifera leaves. Moringa oleifera is a plant that grows in tropical and subtropical areas of the world. Its leaves are rich of nutrients and bioactive compounds. However, several differences are reported in the literature. In this article we performed a nutritional characterization and a phenolic profiling of M. oleifera leaves grown in Chad, Sahrawi refugee camps, and Haiti. In addition, we investigated the presence of salicylic and ferulic acids, two phenolic acids with pharmacological activity, whose presence in M. oleifera

leaves has been scarcely investigated so far. Several differences were observed among the samples. Nevertheless, the leaves were rich in protein, minerals, and β-carotene. Quercetin and kaempferol glycosides were the main phenolic compounds identified in the methanolic extracts. Finally, salicylic and ferulic acids were found in a concentration range of

0.14–0.33 and 6.61–9.69 mg/100 g, respectively. In conclusion, we observed some differences in terms of nutrients and phenolic compounds in M. oleifera leaves grown in different countries. Nevertheless, these leaves are a good and economical source of nutrients for tropical and sub-tropical countries. Furthermore, M. oleifera leaves are a source

of flavonoids and phenolic acids, among which salicylic and ferulic acids, and therefore they could be used as nutraceutical and functional ingredients. I think the following below links and the attached file may help you in your analysis:

https://www.researchgate.net/publication/273423091_Determination_of_flavonoids_by_LCMS_and_anti-inflammatory_activity_in_Moringa_oleifera

Thanks

Moringa oleifera is potential as an absorber of organic pollutants in the simulation solution. To prepare the samples you can follow the following steps: 1) Removing the shell 2) Kiln drying at 50 ° C 3) Analytical grinding mill 4) Screening 5) Storage in the desiccator 6) Dilution with deionized water (efficient concentrations: from 100 to 500 mg / L)

You can build on the methodology of this work

Link:

You can will investigation diesel engin with biodiesel in condition lab scale with real results

Thank you Dr. Majid Abdulhameed Ibrahim for your suggestion

Table 3 and 4 of “Seed yield components, oil content, and fatty acid composition of two cultivars of moringa (Moringa oleifera Lam.) growing in the Arid Chaco of Argentina”

The correlation studies in 34 moringa genotypes indicated strong association of number of pods/tree, number of seeds/pod, pod length, seed yield/tree, 100-seed weight and seed oil content with oil yield/tree. Genotypic and phenotypic correlation coefficients estimated for various traits indicated higher magnitude of genotypic correlations than phenotypic correlations. The difference between genotypic and phenotypic correlations was in general low, indicating the least influence of environment on these traits.

See following publications:

You most welcome

Houda

The fixation in the percent adsorption of MB may be  indicating saturation of all the binding sites on  surface of Moringa oleifera  seeds 

I will strat with a simple hypothesis and methode. You could use a filtered bacterial suspension throught a charcoal filter then put it on a 0.22µm mitrocellulose filter and place the two filters onto a solide agar plate (nitrocellulose in touch with the agar). After incubation, remove the charcoal filter and stain the bacteria using live/dead fluorescent dye  and observe using epifluorescence or CLM.

It's one technics to obtain biofilm on a fiberglass filter which is placed onto a 0.22µm filter before incubation. Here, you will use the charcoal filter instead of the fiberglass. 

DEAR CHINDU

PLEASE GO THROUGH ATTACHED ARTICLE. ''Interaction of Moringa oleifera seed protein with a mineral surface and the influence of surfactants '' THIS DESCRIBE DETAILED MECHANISM OF ACTION OF MORINGA PROTEIN.

Thanks

Dear friend,

As you know very well, every time that you want to examine an herbal extract or seeds you should know what are its ingrediants, for example if it has antioxidant characteristics it may be improve your parameteres, in the other hand, i believe that every feed is poison (over dosage has negative effects). Then at first you must find its ingrediants. We did some expriments in this area.you can read them.

Best wishes,

Mehdi

Thank you so much. I would like to ask is it possible to have a lot of residues left after fractionation by using 4 solvents( hexane, ethyl acetate, butanol and aqueous) ? What I mean is fractionation suppose to separate most of the residues according to polarity right? The residues that are not separated after fractionation done means what?

If you are removing UV254 compounds, Moringa oil could increase the organic content

Dear Ms Aguilar,

Please refer to a paper by Kundu, M., Seed Sci. & Technol., 37(3), 2009; Effect of seed maturity on germination and desiccation tolerance on Moringa oleifera seed.

If the sample holder is Aluminum one would expect sharp reflections with Copper radiation at:

38.47 100

44.72 48

65.095 31

78.23 36

where the second column indicates the relative intensities.

Marcelas angles of 36,4 and 42,7 are rather far away for a good (?) diffractometer. As both reflection are off by 2 degrees, Aluminium seems unlikely to me. Of course, if the sample does not absorb the x-rays very well the sample holder is hit at a lower position compared to the sample. this will shift the observed reflections to lower angles. In my experience though, a shift of 2 degrees would go along wiht a widening of these reflections.

Marcela, you need to specify more accurate information:

What are the relative intensities of these reflections?

What is their width expressed as full width at half maximum. For a good diffractometer these width should be around 0.2 degrees.

Are there further sharp reflections?

If so do they match the Aluminium positions with similar shift and relative intensities