Science topics: Bubble
Science topic
Bubble - Science topic
Explore the latest questions and answers in Bubble, and find Bubble experts.
Questions related to Bubble
Hello
How can I get the velocity plot u(t) of a rising bubble using COMSOL like in this tutorial https://www.google.com/url?sa=t&source=web&rct=j&opi=89978449&url=https://doc.comsol.com/5.6/doc/com.comsol.help.models.cfd.rising_bubble_2daxi/models.cfd.rising_bubble_2daxi.pdf&ved=2ahUKEwj72fCBlPeGAxXEAtsEHcouCCYQFnoECCIQAQ&usg=AOvVaw0KNnlMF2GFBm7hHgylNWEC
A major drawback of applying low-frequency ultrasound and exploiting the cavitation phenomena is the increase in temperature due to the collapse of the bubbles. These collapses lead to the formation of hot spots of high temperature (>5000 K) and high pressure (>1000 atm).
I have seen two kinds of explanations given for the phenomenon that I try to draw in the attached png document. One explanation says that an air bubble creates the experience of a solid black circle at the bottom of the visual field. This might occur when an injection of medication such as Avastin is given that happens to have had some air in the syringe. The other explanation that I have seen says that silicon lubricant intended to facilitate movement of the plunger in the manufacturer-provided syringe flowed into the medication, so there is a bubble, not of air but of some fluid. (Avastin obstructions are described in many places on the Internet, but I'm trying to understabnd a different injectable medication I received,)
I have tried to experimentally produce shadows of air in test tubes, and such bubbles create shadows that are hollow circles.[ EDIT: My experiment was inadequate if, as I believe, the smaller the bubble of air the darker and more equally dense the shadow will be from side to side,] The fact that the circular obstruction in the visual field is not completely opaque and permits reading of text on a field that is dark but not black or opaque, and the fact that the letters of the text are distinct and not distorted, makes me think that whatever was introduced into the eye (and gradually decreased in volume over the next 24-36 hours) may well have been a foreign fluid. Whatever it is appears at the very bottom of my visual field, no matter how I have positioned my head, so I conclude that it must be a single globule that floats at the top of the normal fluid in the eye.
I'm waiting for some test tubes to try working with various oils. Thanks for any insights that researchers with expertise in this area may be able to provide.
Where to buy nano-bubble generators? The bubble size of less than 30 nm is preferred.
Thanks to all
I am facing an issue with how chromatograms are recorded. From time to time, on our chromatograms the peak assigned to heptane is integrated as well and this gives us wrong results. Sometimes the value of this peck is less than 0,01 %, but there are situations when it is 20 % . I made no changes for the methods. I don’t know why this problem appears randomly and what can be the cause? Do you think that it could be a bubble of air, the manual injection or may be is the column? On this GC, we have another column with automatic injection, and this problem never arrived on this one.
Hello Researchers,
I'm making a composite using PEDOT:PSS as a conductive filler and PDMS as a stretch elastomer to make a conductive patch for healthcare monitoring.
The mixing of the two is very good using surfactants, etc., but make it into a film, bubbles continue to form in the film.
This is the same situation when I dry it in a vacuum oven or in a normal oven. Do you know how to solve this problem?
I would be interested in your opinions and own experiences on Jaron Lanier and Tristan Harris' case on how social platforms and the modern age of (monetized) big data manipulate behavior and deteriorate the ability to think critically in an individual.
I don't mean how sophisticated algorithms suggest the best-placed advertisings, that's a commonly known fact and everybody is aware of that nowadays.
What I mean is the perceived "stupification" of younger generations, fueled by overuse of technology in general, and social platforms in particular. The sophisticated tailoring of those platforms to the short attention spans, the mainly senseless content, the biased bubbles and so on, to me, appear to lessen the critical thinking abilities (and -willingness), paired with the Dunning-Kruger-Effect, that those who are the least informed have overwhelming confidence in their ill-informed opinions.
I have a very strong opinion on this, but it may also be biased by experiences I have made throughout the past years as an educator, and deterred by the sort of students I have worked with. I would like to see a bigger picture, not research trends.
So, what are your subjective impressions on the phone- and social platform addicted young generation and their cognitive abilities, their willingness to achieve excellence, and the influence new media has on them?
I have a transparent pipe which takes water from a tank.
The flow happens thru a pump. Pump takes water from tank and makes it flow thru the pipe.
The water stored in tank is open to atmosphere.
When I see the flow through the transparent pipe, i observe very huge bubbles. I have attached an image for your review.
I am interested to learn: from where is this air coming in the flow?
I have some trouble while using the Emulsiflex B15.
Since last week, if i turn the air control knob to fill the sample inside the homogenizer, the sample does not enter well. And even if it enters, it is sucked in with a very very slow speed. The same condition occurs when i try with distilled water instead of sample.
I kept washing the machine with water and ethanol, checked the valve knob at the bottom, and checked the presence of air bubbles in the sample, but it doesn't work. How should we solve this problem?
let me know the antifoaming agent used in ophthalmic preparation to remove the bubbles in preparation.
I am using JACO HPLC and I am unable to get rid of the air bubbles after sonicating the HPLC solvent filter with methanol. What could be done to get rid of the air bubbles in the pipes?
In comparison between simple distillation and vacuum distillation, in which one will the water boil more vigorously (large number of bubbles burst during boiling)?
Hello,
I am using RNAscope® Multiplex Fluorescent Reagent Kit v2 on 14 mikrometer thick brain sections mounted on super frost slides. Recently, I encountered a problem which was not an issue before. After applying RNAscope hydrogen peroxide, bubbles appear on the sections. As far as I can tell, they form also underneath the sections. As a result, I lose if not all, most of the sections on the slides. I would really appreciate if you can help me to identify the problem and eventually solve it.
Thank you very much!
Best,
Firdevs
10mL of MMA monomer that had undergone NaOH to remove the blocker, 20mg of AIBN, and 20μl of n-butyl mercaptan were added to the flask. The flask was first heated in a 60°C water bath for 1 h. Then it was transferred to a 90°C water bath for 30 min. After that, it was heated in an oven according to 50℃-12h, 80℃-12h, 120℃-12h. A lot of bubbles are produced when cracking the test tube and taking out the preformed rod for drawing, we suspect that it is the unpolymerized monomer, is there any solution for this?
I am performing bi-layer well diffusion antibacterial assay of plant extracts. At the bottom of the plate, I pour hard agar media ( nutrient broth + 1.5% agar). Then, I mix bacteria from broth culture with soft agar media ( nutrient broth + 0.8% agar) and pour it on top. In the soft agar, I make well where I load the sample. But only when using gram-negative bacteria, I get this kind of bubbles (apparently) in the media. There has been no problem with bacterial growth. The same thing happened with the TSB media.
On the cell surface, there are some circle-like bubbles.
Is this contamination?
I want to know about that.
Thank you.
Estimation of the Bubble Point Pressure of Multicomponent Reservoir Hydrocarbon Fluids
"Quantifying Bubble Generation in Electroplating: Nickel-Boron (NiB) Solution with Copper Cathode and Nickel Anode"
In my electroplating experiment involving the deposition of nickel onto a copper cathode in a nickel boron (NiB) solution with a nickel anode, I have observed bubbling at both the anode and cathode upon initiating the circuit. I am interested in quantifying the number of bubbles generated during the electroplating process as a means of understanding and optimizing the system. What methods or techniques can be employed to approximate the number of bubbles produced? Are there specific instruments or analytical approaches that researchers commonly use for this purpose? Additionally, any recommendations for relevant literature or studies on quantifying bubble generation in electroplating would be highly valuable.
From the literature, for instance, bubbling Nitrogen gas containing 5000 ppm H2S in deaerated solution will produce a 0.5 kPa H2S partial pressure. Do we have to keep bubbling the gas in order to have a constant 0.5 kPa partial pressure of H2S in the solution.
Can we do it in another way by pressurizing the system at a certain pressure via closing the system and keeping the gas cylinder open to the system.
Is there another way other than continuing bubbling?
I have a western blot that I am visualizing with a colorimetric solution and I have no bands. I know there is something on the membrane however, because I stained it after transfer. I blocked in skim, applied primary 12-24 hours, applied secondary 75 minutes, and visualized. On the first trial secondary stayed on quite a bit longer, and I had no result. Nothing at all showed up on the membrane. The second trial, I did the secondary for exactly 75 minutes, visualized, and nothing showed EXCEPT my ladder. So, now I know the secondary and visualization worked, but maybe not the primary? I have never had issues with this primary before, so I am not sure. Aside from there simply not being any of the specific protein on there, I am trying to look at other angles first. I can attach my pictures of membranes after visualizing, the first trial is the cloudy/bubbly looking one, the second is the one with the ladder.
I have start working on the study of flow pattern of multiple multi-phase flow pattern. I am a lit bit confused in the calculations of pressure drop in the case of bubbly to annular flow and compare it with the pressure drop in the case of swirl flow without having different phases(regimes) between bubbly to annular flow. Can some one help me regarding this study?
Hello everyone,
I've encountered an unexpected issue while attempting to multiply bacteriophages from soil samples that were previously isolated. During the double-plate assay, I observed the formation of bubbles. Initially, I assumed they were typical bubbles caused by improper preparation of the culture medium. However, these bubbles exhibited an inhibition halo against Pseudomonas syringae, and over the course of three days, they have grown, burst, and given rise to new ones.
I hypothesize that this could be a contamination from a fermentative bacteria or possibly the bacteriophages themselves being carried by the bubbles. However, I'm not entirely certain. Any suggestions or insights into this phenomenon would be greatly appreciated.
Whenever I do a western blot, when the comb comes out of the stacking gel, there are little bits of gel stuck in the wells and some bubbles. What is the best way to get rid of the debris and bubbles?
Upon adding CCl4 (Carbon tetrachloride) to my in vitro sample, I noticed bubbles or maybe the plastic being degraded at the bottom of the 12 well plate. The concentration used was 4mM, which is in the normal testing range.
The first time and third time I used the chemical, I had no issues. The second, fourth, and fifth time, I see the bubble formation, which seems to 'trap' a few of the floating cells in the center of the 'bubbles'. Attached is quick image for reference.
The drug is being taken out with a needle through the Sure/Seal system. Any advise/know what the problem is?
I am trying to grow microalgae. Between bubbling in air (just air, no concentrated CO2) and shaking, does either of them help grow algae faster? Or do they essentially have any fundamental differences between them?
Also, does anyone have any recommendations for set ups to bubble in air or CO2? (I am in the US)
Thank you!
J.R. Grace, T. Wairegi, T.H. Nguyen, Shapes and velocities of single drops and bubbles moving freely through immiscible liquids, Trans. IChemE. 54 (3) (1976) 167–173.
Grace, J.R. (1973) Shapes and Velocities of Bubbles Rising in Infinite Liquids. Transactions of the Institution of Chemical Engineers, 51, 116-120.
Dear all,
since Twitter was taken over by Elon Musk, a lot of researchers have left that social media. I too no longer actively use twitter, although I am kind of sad because it was a great platform to foster connections with researchers outside your typical bubble and also engage with laypeople. You were also able to package your research into small, easily digestible tidbits by creating sharepics or max. 240 char long summaries...
Anyway, where are you doing scicomm now? Is it mastodon? LinkedIn? Or something else entirely? And how would you judge your outreach possibilities there? Researchgate is great for reaching my direct colleagues, but real scicom needs to strive for more, imho...
Hello dear friends
What is the the dew point temperature of water?
At a constant pressure, the temperature of the vapors must be lower than the dew point temperature for all the vapor to condensate? Or must the temperature be lower than the bubble point temperature of the vapors for this to happen?
Hello all dear
Is it true that when the temperature of the solvent vapor is lower than the dew point, the first drop of liquid is formed, and if the temperature is reduced, the amount of condensation formed increases? And if the temperature is below the bubble point, will all the evaporating solvent condense?
(All temperature reductions should be at constant pressure)
Hello dear all,
We want to set-up a CFD -Discrete Particle Modelling case for a bubble column where liquid is eulerian and gas bubbles and solid particles are discrete phases (three-phases total holdup <10 %) as given in attached diagram. In this case only gas bubbles are flowing with velocity UG <1 cm/s while liquid and solid particles have no initial velocity (means batch mode). Boundary condition (DPM) for gas phase at outlet is pretty straight forward "escape" but we want to retain second discrete phase "particles" with in column volume. However, Boundary condition panel in Ansys Fluent do not show "escape" or "wall" or "reflect" for an individual DPM (injection) phase but one for all DPMs.
We want to model the effect of gas bubble induced flow behavior (in one-way coupling) on liquid and solid phases, so we need to keep liquid in batch mode and solid phase (one-time injection). Any suggestion on how to set-up DPM-BC for two discrete phases (two different injections) separately in Ansys Fluent?
Hello,
I am using the costar gel loading tips round 4853 and an eppendorf pipette (100 µl) to load my gels. The volume of my samples is 25 µl. Before loading, I usually set my eppendorf pipette to 25 µl and use the gel loading tips as mentioned above. But everytime I get air bubbles in my tips that result non-optimal western blots.
Do you have any tips/ideas how to avoid air bubbles in the tips?
What I've already tried:
I resuspend the tip to remove the air bubbles but in most cases the resuspending process results in more bubbles.
I pull the pipette slowly to avoid capture any air, but still isn't working.
I readjusted the volume of the pipette to 23,5 µl to set a lower volume to avoid sucking excess air, but then I lose sample since a small amount is still in the tube. So that doesn't work either.
I am thankful for any help and tips!
Recently I noticed that air bubbles constantly appeared from my pump and keep clogging my columns.
【Solvents】
- Solvent A =sodium acetate buffer (0.05 M, pH 6.0) in water containing 5 mM β-CD (Sigma-Aldrich, UK)
- Solvent B=sodium acetate buffer (0.1 M, pH 6.0 anhydrous) in a water:methanol: acetone mixture (volume ratio 20:72:8).
【degas and filter situation】
- Because of the degasser damage, I only ultrasonic degassed my solvents for 20 mins.
- filtered properly
【Issue】
When each solvent went through the single channel, there were no air bubbles (e.g. Flow 1.2 channel A 100%). However, when I chose 80%A+20%B, Flow 1.2, it generates tiny air bubbles. Please see the photo I took.
【potential answer】
1. solvent is still not enough degassed
2. the piston or seal in the pump was worn
Hello All,
I try to mold 2% sio2+PMMA nanocomposite powder into the tensile specimen. The hot press temperature is 130 C and I usually add 2 to 3.5 Ton bar on the mold for 15min ( preheat the mold, put the mold and sample in the hot press for 3 min without P, and then apply pressure and then let it cool down at room T).
I use the same condition for pure PMMA and I don't have any bubbles on my samples however for the nanocomposite, I try different things such as the above method or adding some powder, applying P, removing it, and adding another layer and letting be in the hot press for 15 min but non of them work and I still have bubbles in my sample.
Any thoughts or tricks to get rid of bubbles?
Thanks,
Sourena
Hi, i m using Agilent HPLC and i m facing a troubleshooting with degasser, the led turns red when i m increasing the flow from 1 to 3 and finally to 5mL/min. Also, when the led is green, i see bubbles after passing from degasser section. There no bubbles from mobile phase solution till degasser, afterwards appear.
Thank you,
The film forming solution (FFS) is stirred overnight and it is oven dried at 40°C.
Please give me suggestions to reduce the air bubble.
Reservoir Geo-mechanics: Biot’s Coefficient
1. How important is the concept of Biot’s coefficient
(involved in Biot’s effective stress relationship which assumes that total isotropic confining stress remains equal to the sum of effective stress and the pore fluid pressure multiplied by Biot’s coefficient)
towards characterizing a petroleum reservoir?
Feasible to determine Biot’s coefficient
for a low-porous and low-permeable reservoir
@ laboratory-scale
considering the time required to reach equilibrium of reservoir pore fluid pressure?
2. Feasible to validate the following two basic aspects
@ field-scale
associated with a petroleum reservoir,
when reservoir pressure remains to be
lesser than bubble point pressure?
(a) Biot’s coefficient cannot be greater than unity, if the reservoir is assumed to be an elastic isotropic material; and
(b) Biot’s effective stress getting reduced to Terzhagi’s effective stress upon Biot’s coefficient reaching unity.
3. How do we know whether the exploitation of oil and gas
at a particular basin has "significantly" contributed to perturbations
in the geosphere in terms of changes to the total stresses, pore pressures and the thermal regime?
Along with in-situ seismic wave velocity measurements, whether the existing coupled effect of thermo-hydro-mechanical-chemical phenomena would be able to provide the required responses of water/oil/gas saturated reservoir rock masses (which essentially depends on how exactly the external stresses remain partitioned between solid-grain network and the reservoir pore fluids)?
4. Although Biot’s theory of poro-elasticity can be expressed as functions of strains, elastic properties, and fluid pressure or increment of fluid volume per unit volume of porous reservoir rock using linear elastic state partitioning; when exactly a petroleum reservoir requires the partitioning between solid-grain network and pore fluids to remain to be defined by a non-linear elastic state under transient conditions (and not under equilibrium conditions)?
5. To what extent, the concept of Biot coefficient
(a scalar multiplier for the pore pressure term in the stress-strain-fluid pressure relationship)
remains to be useful in characterizing conventional hydrocarbon reservoirs?
How easy would it remain to measure effective stress coefficient
(the pore pressure factor associated with the stress regimes that falls outside Biot’s linear poro-elasticity)
below and above bubble point pressure?
Whether the same simplified concept (linear poro-elasticity) could be extended to unconventional reservoirs as well?
6. Bulk compressibility being a function of pore-shape, fracture aspect ratio and fracture density, how easy would it remain to determine Biot’s coefficient of a fractured reservoir?
Whether Biot’s coefficient would remain to be varying as a function of
(a) stress path; and
(b) fracture orientation (with reference to their bedding planes)?
I ma doing a 25ul reaction for my qPCR. I know that making bubbles would destroy my results as it makes DNA polymerase does not work.
I use centrifuge after I am done with pipetting but it is not a 96-wells centrifuge.
What should I do to do not have bubbles then?!
thanks
I'm trying to manufacture microfluidics into 75x25x1mm COP polymer slides. The process is 11 min long and using 150°C (COP Tg=100°C) temperature around 20-30bar pressure.
Problem is during the process a lot of bubbles forming at the contact area between the tool and COP slide and in the COP slide as well.
First guess was moisture/entrapped water but the effect still remain after drying the slides.
There are no additives in the polymer as long as i know, only COP.
Thanks for the help!
Hi,
I am trying to understand the impact of inlet gas pressure on the OTR in a bioreactor. I understand that the oxygen concentration in the gas depends on the pressure, so at higher inlet gas pressure, you are supplying higher concentration of O2. One thing I am trying to understand is does the inlet gas pressure have any impact of the bubble size, superficial gas velocity, etc?
Thanks!
U-slip is used to calculate the drag coefficient in EMMS/bubbling model. But there are three different slip velocities in the model. I do not know which one should be chosen here. And my calculation results are a little bit different from the references.
The references I used are as follow:
[1]HONG K, SHI Z, WANG W, et al. A structure-dependent multi-fluid model (SFM) for heterogeneous gas–solid flow [J]. Chemical Engineering Science, 2013, 99: 191-202.
[2]SHI Z, WANG W, LI J. A bubble-based EMMS model for gas–solid bubbling fluidization [J]. Chemical Engineering Science, 2011, 66(22): 5541-55.
First, there are bubbles with small black dots inside visible. The next day those bubbles disappear and the rod-like structures are present in our cell culture, which are fast growing but not moving. We use 1 % Pen/Strep in our media.
Does anyone know what kind of contamination this is?
I've researched some papers, most refer to bubble/water dynamic simulation with species concentration variation use VOF method, just one by phase field and only study the surfactants distributed along the interface between bubble and water.
I was forced to study the bubble/water dynamic simulation with species concentration variation distributed in the whole water region without bubble, my tutor is averse to permit me to research it by any methods other than phase field. Any friends would like to give me an explicit answer whether it works or any recommended papers to persuade my tutor?
Very appreciate it!!
Is lower density liquid residing/flowing in a higher density liquid considered a bubble or it is considered as droplet? Or bubble is just gaseous by definition?
Hello,
I wanted to plot a graph of the bubble size distribution from this image.
This image is taken from a slow-motion video (the video was taken with a mobile phone) where the bubbles enter from the bottom to the top.
Various software such as Image J, Fuji, bubble analyzer and Motic Imageplus 3.0 were used to analyze the image. However, I could not accurately identify bubbles with these programs. (Of course, if I have done things correctly with the software)
What program do you recommend for checking this image for all bubble identification?
According to the image, do you think it is possible to detect all the bubbles using the mentioned programs?
Or do you know another way with a mobile phone to take pictures of bubbles moving at high speed?
Thank you in advance for your guidance in this matter.
Hi everybody
I tried to work with 1-octanol in order to avoid bubble formation during the whole digestion process. However, it did not work since during digestion with sodium hydroxide there was a huge bubble formation and the food sample gets trapped in the upper walls of Erlenmeyer flask. What antifoaming agent do you recommend?
Thank you very much in advance.
I am working with PEN membrane slides used in laser capture microdissection. When I try to cut the cells on the membrane, too many bubbles are seen. I made a tiny hole on the edge of the membrane and water came out. I did dehydratation steps after IHC and put the slides 60 C inside the hot plate to make them dry but I couldn't get rid of water without making a hole on the membrane. Do you have any idea what could be the reason of the water or any suggestions to make water-free slides? TIA
I made my ligations using pGEM-T easy vector system I, I normally used PCR products (product for TA cloning-Taq DNA polymerase and Control Insert DNA (promega like a ligation control) for the transformation I used uncut plasmid like I control. But after I have plated bacteria and left them in 37C incubator overnight I do not have colonies just find circular water bubble in my LB agar with amp (PCR products and Control Insert DNA) but in my transformation control I have colonies. this demostrated that transformation process is fine and my cells are competent. Is possible that reagents of this lot it is degraded? T4 enzyme for example. I repeat many times following the insert kit protocol, What is causing these water bubbles?. When I prepared my plates with LB media I avoid the bubbles all the time.
Hello everyone,
My question is about the flow exceeding the set value in the Knaver HPLC pump. By setting the flow pump to a specific value and opening the pressure relief valve, the flow output from the pump is much higher than the set value. The output value is close to the value that the purge button is pressed for bubble removal. This amount does not change with the change of flow.
I would be grateful if you could help me find out what the problem is.
As to the title, I have computed a gas-liquid two-phase flow by "laminar-flow,level-set ". I wish to get the velocity of the head of the bubble vs. time, i.e., the velocity of the interface(phils=0.5). How could i get this velocity?
Thanks a lot.
How they are related and what is the differences?
In an age of residing within digital bubbles, alienation and isolation have become the 'new normal' prominent stages of our lifestyle. In this manner, our own space can be perceived as the illustrative outcome of our escapism from reality.
A Foucauldian approach to such phenomenon is the 'Heterotopian' lifestyle.
What do you think would his views on the metaverse be if he was among us right now?
To what extent will he support or reject the idea?
How would he contextualize the metaverse within the current social, political, and economic conditions?
Hi,
i have a question about my model for the thesis.
I have identified the periods that go back to the bursting of bubbles within the series of oil and natural gas prices through a methodology. I would like to include the variables =1 in the presence of a bubble and =0 otherwise in a VAR model. I would like to estimate a model that allows me to understand how the aforementioned bubbles influence the price of the US stock market index.
I am doing IHC with mouse cornea and after adding Prolong Gold Anti-fade mounting media, there are now bubbles on the slides. How can I remove the bubbles?
Thank you!
I tried to use hydrogel as bioink to print a structure. However, there's always bubbles in the extruded solution even though I have already degased the ink by using vacuum.
Question: During boiling point determination, our glycerin start bubbling at 255 oC but temperature does not be constant and continuously vary, at 270 oC bubbling stopped but temperature vary is continue till 280 oC.
We want to obtain highly porous pastes without resorting to chemical methods such as the use of expanding agents.
I recently have realized that I have air bubbles on my tissue sections before I add a drop of mounting solutions to tissue. And it seems like bubbles are existing between tissue and slide that cannot be removed even I try to wash it with icy cold PBS solution.
I'm happy to accept your suggestions and hope you are having a great new year!
Dear all,
I'm validating the Shan-Chen model of single bubble evolution with Rayleigh-Plesset equation, However, I tried my best but cannot get close results with theory Rayleigh-Plesset equation (in literature it always have good agreement) which I solved with Runge-Kutta. I tried different domain size and with larger size it seems the pressuer boundary condition has less effect on the bubble. I also tried different literatures with different R-P equation and the results differ. Can someone give me any directions on how to fix this problem? The picture is attached as follows and thanks in advance.
Hi,
I am modelling an Alkaline water electrolyzer by using multi phase model in porous media. I am little bit confused about volume fraction is that does the gas volume fraction represents here only the undissolved gas (bubbles) or both dissolved gas and undissolved gas (bubbles)?
Please someone can provide me clarity about this or recommend any reference which I can go through.
Thanks!
Dear all,
I am having a technical problem with our FACS Canto II. It seemed somehow the device picked up some air bubbles and now it does not complete the fluidics start up neither the purge. I tried doing SIT flush several time and degas the flow cell. Both were completed successfully yet still the device kept giving the red alarm that fluidics start up can not be completed. I tried then Bubble filter purge several times yet it failed all the time. Any recommendation or tipps how to remove air bubbles from the system?
Thanks in advance
We have a problem in preparing the standard NACE test solution “A” for sulfide stress cracking (SSC) testing. I am trying to saturate a solution containing 5 wt.% NaCl and 0.5 wt.% CH3COOH with H2S by bubbling a nitrogen gas containing 95 ppm H2S. The obtained pH was 2.7 compared to 2.6-2.8 in the NACE standard. The NACE standard recommends continued bubbling during the experiment, but we do bubbling for about 1 hour per day. The system is kept close after the bubbling and nothing can escape. We have a problem in getting SSC in the test specimens, although we have applied a tensile force very close to the braking force.
Do we have to use pure H2S gas instead of 95 ppm H2S?
Do we have to make the bubbling continue during the experiment?
Hi
I am trying to simulate evaporation of gas R134a using Lee model in VOF, the problem is during the boiling process the bubble forms but the temperature of the liquid is increasing more than the saturation temperature though it should be constant during the boiling process. So why this happens is there something need to be defined.
Thanks
Hi,
I am modelling an alkaline water electrolyzer by using multiphase flow and species transport physics.
I am confused between gas and bubbles terms. From multiphase flow I can simulate the gas and liquid phase. While, with species transport I can simulate gas concentration in liquid and bubble nucleation.
Is bubble nucleation indirectly tells about gas region?
Hello everyone,
I am trying to learn the fundamentals of breakage and coalescence modeling in bubble columns, specifically, for air-water systems when the water is at rest. I believe this is one of the most basic forms in bubble columns however, I am having a hard time finding good references on the topic.
Please let me know your recommendations for any good reference.
Thank you,
Erol.
Hello dear RG community.
I'm trying to identify contours of bubbles on the images taken with Nikon 1 J4 camera ( backlit through a diffuser with a halogen lamp). I use Python.
I'm trying to go with Watershed, but both opencv and skimage make use of binary markers to assist Watershed.
Binarization of bubble images doesn't suit that purpose well. Moreover, if I was able to obtain good results I would just go with binarization instead of finding contours.
I haven't been able to find Watershed codes without markers.
I'm wondering if anybody has an open source code for Watershed without markers.
And in general, I'm wondering if anybody has come up with a code to identify bubbles contours or just bubbles in a wide range of two-phase flow regimes and if the code is open source.
Thank you in advance.
Ivan
Hi everyone!
I'm simulating a two-phase flow (liquid and air).
I have the volume-of-fluid field as well as the level set fields. I want to make a plot in such a way that I can identify isolated air bubbles. I tried plotting contours of level set=0 AND contours of VOF=0.5, but those are ambiguous and I think they don't necessarily show the exact bubbles in the domain.
Does anyone have any idea how to do that?
I hope I was clear. Thanks!
After pouring media in plates there are no air bubbles but after streaking culture on the media plate usually found some air bubbles, is there any particular reason behind that?
I have used the Quant Studio 3 instrument and the Taq Man Genotyping Master Mix from Applied Biosystems for a genotyping PCR reaction, but I got any signal, even not of the reference dye (ROX). After the PCR run the tubes were ok with the expected volume (11 uL), without bubbles, droplets or condensations. What could be the reason of this result?
The master mix was new but I have to add that it were accidentally stored at -20 ºC for a period of a month and the instructions were storing at 2 to 8ºC, could be that this has been spoiled?
I am currently using Bernouilli's Equation to determine the work required on a pump to maintain a flow rate through a system, in the attached image. In this flow system a stationary bubble is present along with other features, sudden expansion, sudden contraction, frictional losses in a pipe with a cylinder inserted in it etc.
What I am wondering is, for velocity of water that is below the propagating velocity of the bubble can you assume it to be an obstruction and the head loss coefficient is as follows:
K(obstruction) = (A/(((A-a)/A)*(A-a)^2)
where a is the cross-sectional area of the bubble and A is the remaining area the flowing water can pass through.
This head loss coefficient will be multiplied by (V^2)/2g, to get the head loss contribution of the stationary bubble where V is the velocity of water at the bubble and g is gravity.
I realise this assumption will only be valid at values of V that do not exceed the propagation velocity of bubble (the velocity of water that creates a drag force greater than the adhesion forces of the bubble in the direction of the flowing water).
Further equations used in this flow characterization can be made available on request.
I am trying to simulate bubble rising condition in a pipe using dam break tutorial file available in openFoam.
I am not an expert guy. Can anyone suggest me to do what steps should I take to complete it?
Recent studies show that stable gaseous nanobubbles can be formed in liquids that form at the interface with solid substrates. But, if the nanobubble is formed inside a crack or cavity, can it be even more stable than a surface nanobubble since two solid surfaces surround it?
I am currently working on foam using dynamic foam analyzer, analyzing the structure of bubbles.
Sauter mean radius is one of the parameters that the instrument provides but I am getting difficulty understanding what this parameter means, if the value is increasing or decreasing with time what interpretation can be made from these values?
Anyone with a Differential Scanning Calimeter (NanoDSC, TA, Instruments) has had erratic peaks during the scans?...
We have use all type of cleaning protocols and eliminated bubbles, but the peaks don´t go away.
Dear All,
Does anyone simulate the Particle and Bubble Interaction in MATLAB?
Please attach it for everyone.
If the question is ambiguous please let me know.
With Bests.
A solution that turns into a gel and under constant stirring, air bubbles tend to stay trapped inside. I tried ultrasonic treatment but it didn't help.
Can you suggest any other technics or tips that help prevent this issue on a lab scale.
Thank you
Many bubbles form when I prepared glassy sample....How I can remove this bubbles from molten glass ? Thanks a lot...
During my cell culture, a large number of microorganisms suddenly appeared in my dish, and they reproduced very fast, like a layer of fine sand when shaking, and like a mosaic under the microscope. They are not black, more like a group of very small bubbles. I'm sure they contaminate my medium because when I culture the medium (without cells) alone, they reappear and multiply.
Has anyone met the same thing?
I'm attempting to tape cast porous ceramics for use in SOFCs. To the ceramic powder, pore former, ethanol, and dispersant solution mixture, I added binders in solid form and ball milled. I deaired in a vacuum desiccator for 30 minutes prior to tape casting, but the slurry is still bubbling up ( as shown in the picture). If I extend the time, it ultimately evaporates as I use ethanol solvent and it gets dried up and becomes unsuitable for casting. After deairing for 30 minutes, I tried tape casting, but the dried sheets formed bubbles.
Could someone kindly suggest me on avoiding bubbles to get flat, uniform sheets?
Thank you
Hi, everyone. I’m doing the lymphatic duct 3D culture recently. I embedded thoracic duct pieces from mice into the collagen type I (rat tail) sandwich. I’ve tried various concentrations of the collagen (0.5mg/ml, 1.0mg/ml, 1.5mg/ml, 2.0mg/ml, 3.0mg/ml), yet every time more than half of my samples formed bubbles around the tissue. And apparently these bubbles have hindered the growth of the sprouts (no sprouts).
To avoid these annoying bubbles, I dropped the ice-cold collagen by invert pipetting directly onto the tissue pieces and then let it sprawl. I also tried pipetting the viscous collagen to the sidewall of the well, but it polymerized and partly attached to the sidewall quickly, which made the collagen cover uneven.
I guess the bubbles were formed from the evaporation or absorption of the medium wrapping the tissue after I covered it with collagen, which left a space between collagen layers. As I took the tissue pieces from DMEM onto the bottom layer of collagen, there was always medium remnant with them. When I observed my samples immediately after I finished the sandwich collagen embedding, there were rare bubbles and every gel seemed perfect. But when I checked them again one night later, bubbles appeared magically, especially around the tissue.
I used 10x DMEM, 1x DMEM, and ddH2O for collagen dilution, and 7.5% NaHCO3 & 1M NaOH for pH adjustment. The assays were performed on ice. Both the bottom and covering layer of the collagen (50ul for each layer) were incubated at 37℃ for 30min. The thoracic duct was cut into pieces in DMEM and then transferred to the gel. I’ve already tried soaking the tissue pieces in 0.75mg/ml of the non-polymerized collagen or cutting the duct in non-polymerized collagen before placing them on my bottom gel layer, which gave me little help. I’ve also tried leaving the tissue on the bottom gel for 10min, in order to let the medium evaporated a bit. This didn’t work either.
Once the bubbles generate, they’ll never be removed. Please help me figure out the right way of making the 3D collagen. Thank you very much!
Is there any possible invertible sorting algorithm for a number series . So given this:
67,66,14,15,106,95,94,101,86,85 .......
full sort would create:
14,15,66,67,85,86,94,95,101,106 .................
And then reversing it without having to know any knowledge, other than perhaps which sort algorithm was used and how many iterations were run, it would go back to:
67,66,14,15,106,95,94,101,86,85
or
partial sort would create:
66,67,14,15,106,94,95,101,85,86 ....................
it would go back to:
67,66,14,15,106,95,94,101,86,85 .........................
Note: Permutation sort is very slow and bubble or any other are not invertible in all cases.
Can we have any other invertible sorting algorithm to fully or partially sort any series ?
The algorithm should be work for all use cases of number series.
E.g. Attached Bubble sort is invertible for this series but very slow for any large such series
arr = [64, 34, 25, 12, 22, 11, 90]
Original array is:
64 34 25 12 22 11 90
Sorted array is:
64 34 25 22 12 11 90
Reverse Sorted array is:
64 34 25 12 22 11 90
But it fails for other series.
Our Effluent treatment (ZLD) plant consist of stripper for solvent separation (bubble cap tray) but it's performance is not reasonable.
Feed COD = 24000 ppm
Distillate COD = 28000 ppm
Feed = 35KLD.
Reflux ratio = 3
Temperature = 115
Steam pressure to reboiler = 0.5
I am performing shadowgraphy imaging of laser-induced cavitation bubbles. I want to know the optimum value of optical density for a NDF in my experiment which can provide me the best image.
I'm currently trying to form a Dextran layer on glass or Si substrate with spin coating.
I've read several other articles doing this and make 300 to 500nm thick Dextran layers.
However, the thickness I need is several um-thick, up to 10um in optimal.
I've tried to make more thicker, denser Dextran solutions up to 50wt% & tried to slow my spin rotation speed.
Right now, these have not shown significant improvements, and also bubbles are starting to form inside the layer, which is clearly not desirable.
Have anyone formed Dextran layers with um-thick? I'm up to any other process than spin-coating, as long as it works.
I am working on Casimir and Double layer force in liquid(water) by AFM, but many bubbles appear after injection of water into the AFM tip holder, I am not able to remove air bubbles from the water. Is there any way I can remove bubbles from water?
Hello everyone,
I am trying to prepare a sample by means of the hot press method for polamide6 (PA6) but the problem is bubble formation. How can I remove bubbles? Also, I put PA6 in a vacuum oven overnight.
Thanks in advance
In anticline structure carbonate Reservoir of areal extension 31x10 km with 24 to26 degrees. no compartments. And no faces alteration (hydraulic continuty exist) multiple Downhole samples, MDT, and well testing samples were taken the bubble point ranging from 2140 psi in one flank to 2746 psi as average.
Dear all,
I was trying to get PVA thin film via solution casting in PTFE petri dish, and the PVA solution is 4 wt% in water. Right after the clear PVA aqueous solution was obtained, it was cooled down naturally to room temperature (RT) and sonicated for about 1 hr in water bath at RT followed by being left for about 48hr to remove possible trapped air before film casting. Such solution casting was baking at 60degC for 24 hr and cool down to RT, there are quite a lot small bubbles noticeable by naked eyes in such as-made thin film. Any other suggestions for removing the bubbles more effectively?
Thank you so much for your time and help in advance.
Yours sincerely,
Xiaosong Liu
Hi all,
Bubbles affects the absorbance reading from my assay. How can avoid bubbles when pipetting with multichannel pipette.
Thank you
I poured 3m 7500 with pico-surf into a closed chamber and heated it to 95 degrees and tried to make no bubbles inside the chamber
Hello there,
I'm trying to measure the maximum theoretical specific gravity (Rice gravity) of some cold mix patching materials. The materials include flux oil and other cutters. During measuring the maximum theoretical specific gravity (Rice gravity) of material, there are many and countless numbers of air bubbles that are not removed even with a vacuum system. It's very difficult to remove the bubbles from the aggregates. In fact, the air bubbles have been stocked with the grains and there is a strong adhesion between them. I used a small amount of soap to help bubbles be released, but it did not work. So, I need some help. Any suggestions?
Thank you in advance.
Every time i working with hyaluronic acid I got some bubbles inside gel. It desn't matter in lab and do not affect on the acid. Few days ago I saw syringe (with a needle) with clear hyaluronic acid without any bubbles. Friend send me a photo of bottle with the product without bubbles too. How do they made it??? I was trying vacuum suction but did not get proper effect.
Hi all,
1. How do you identify if you have bubble library or just insufficient size selection?
2. How to deal with bubble library? Would reconditioning PCR work? Is it recommended to use single primer or both forward and reverse primers?
3. How to quantify bubble library for library pooling? qPCR? How?
Thanks.
Lux
I am culturing some patienet-derived primary lung cancer cells in vitro .Recently I found there was some bubbles in several flask of tumor cells, with no matter high or low passage numbers.Some of these have no inner strcture, but some of these bubbules have tumor cells inside.Also I could see some bubbles floats in medium.So is this phenomenon an appearance of cell senescence? I was wondering does it means these cells are in a bad state?
Thank you for your answer!
There's a weird issue. I have a stim box, which has 2 entrances, one called "act.", and another one - "ref". So if I plug a bipolar electrode in it, so 1 electrode goes to "act." and another to "ref", I see bubbling on only one tip, instead of seeing it on both. If I exchange places, where electrodes connect, I see bubbling on another tip. As I know, it should not be so.
So, do you know, what's the problem here?
I have found a lot of related 2D bubble simulations, but I can't find the settings for 3D model bubble simulation. I want to ask how to set it up, and how to fully present the bubbles in CFD-POST?
Hello out there! :-)
I am doing a lot of Western Blot and sometimes annnoying, irregular spots become visible on the PVDF membrane during visualization using ChemiDoc (attached file).
(Here some steps I regularly do regarding the membrane:
the PVDF membrane was freshly cut from the membrane roll (bio-rad), activated with 70% methanol for 30 sec, any air bubbles were removed by a roller during sandwich assembly, the wet transfer was run (40V, 2.5h, stirring, cooled) in 1x carbonate buffer (3mM Na2CO3, 10mM NaHCO3 in ddH2O). I made sure that the membrane didn't dry out during those working steps.)
Do you have any ideas where these spots come from? Did this ever happen to you as well?
I totally appreciate if you type here some ideas or share your experiences.
Thanks a lot!
All the best from the lab,
Judy & Kathi
## Western Blot is trouble-free. Said no one ever! :-DD
Hello, everyone. I would like to determine the fiber type of each skeletal muscle fiber based on its SDH and ATPase activity.I used the dry ice-hexane method to freeze freshly isolated skeletal muscle because the liquid nitrogen freezing method produced bubble artifacts. Although these methods were used to evaluate by enzyme activity, there is unavoidable bubble artifact.
If it is difficult, I will prepare frozen sections after saturation with normal sucrose and use an antibody-based staining method for each muscle fiber.
If you have any knowledge, I would appreciate it very much.
Thank you in advance.
