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Biogas Production - Science topic

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Hello! Our current project is to create a plant/equipment design on biogas production from food waste from food waste generated by a specific restaurant. The data they can provide is only the weight of the food waste they generate per day.
Our PFD includes size reduction, hydrolysis tank, anaerobic digester and scrubber system.
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Try to make some assumptions based on bibliography about waste composition of your kind of source. Focus on similar origin, geographical proximity, etc. Good luck!
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Are there technologies available to produce biofuels based on organic compost, from organic crop waste, that could serve as engine fuel to power tractors and other agricultural machinery in sustainable, organic, pro-environmental, pro-climate farming?
In recent years, in connection with the inclusion of the agricultural sector in the process of green transformation of the economy as well, new solutions are being sought, created and implemented to enable the transformation of productive farms into sustainable, pro-environmental, pro-climate, organic farms growing crops and other aspects of agricultural activity in accordance with the principles of organic farming and with the simultaneous implementation of sustainable development goals. Accordingly, on a farm operated in accordance with the formula of sustainable organic agriculture, pesticides and other chemical pesticides and chemically produced fertilizers are not used in agricultural crops, energy is obtained from renewable and emission-free energy sources, water is obtained mainly from rainwater catchment facilities, organic manure and processed organic waste from composters are used to fertilize farm fields, crop rotations are used within the framework of multi-year cycles to restore high soil quality and prevent soil aridity, individual farm fields are separated by green belts and flower meadows to provide foraging areas for pollinating insects, on-farm mini-treatment plants are built to treat wastewater generated on the farm and not used in a specific way for farm purposes, etc. Besides, on a farm run according to the formula of sustainable organic farming, available technologies for the production of biofuels based on organic compost, from organic waste from agricultural crops, through which motor fuel is produced to power tractors and other agricultural machinery, should be used. These may include the construction of biogas plants, where biogas can be produced, which can then be used to power agricultural machinery. Besides, in a sustainable, ecological, pro-environmental, pro-climate farm, agro-tourism activities may also be developed, and certain preserves, food products, such as jams, juices, cheeses, etc., may be produced within the framework of a micro-production activity or manufactory, within the framework of local brands on the basis of organically produced, cultivated crops. In addition, such sustainable, organic agritourism farms may be associated within a local producers' cooperative with silos, warehouses, etc. in which crops from the cultivation of several or more locally operating farms and/or agritourism farms are stored, including or mainly, only those operating under the formula of sustainable organic farming.
In view of the above, I address the following question to the esteemed community of scientists and researchers:
Are there technologies available for the production of biofuels based on organic compost, from organic crop waste, which could be used as motor fuel to power tractors and other agricultural machinery in a sustainable, organic, pro-environmental, pro-climate farm?
Could biofuels from agricultural waste be used as engine fuel to power tractors in sustainable organic farming?
And what is your opinion on this topic?
What is your opinion on this issue?
Please answer,
I invite everyone to join the discussion,
Thank you very much,
Warm regards,
Dariusz Prokopowicz
The above text is entirely my own work written by me on the basis of my research.
In writing this text I did not use other sources or automatic text generation systems.
Copyright by Dariusz Prokopowicz
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Yes. It has huge demands as alternative energy generatin (SDG 13)
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What role can biogas plants play in the development of sustainable, emission-free organic agriculture as an important element in the green transformation of the economy and the decarbonisation of civilisation's economic processes and in counteracting the progressive process of global warming?
In order to slow down the progressive process of global warming, it is necessary to increase the efficiency and accelerate the implementation of the green transformation of the economy in order to create a sustainable, zero-emission, green circular economy as quickly as possible. In the process of efficiently implementing the green economy transition, there are several essential components, which include the green energy transition based on the development of renewable and zero-emission energy sources, the development of electro-mobility, the development of sustainable organic agriculture, sustainable energy-efficient construction, low-carbon mining and industry, etc. The development of agricultural cooperatives that run sustainable business ventures supporting farm management, the development of composting facilities, the improvement of the post-agricultural waste separation system and increasing recycling, the development of small-scale renewable and zero-emission energy power plants supplying energy to farms, and the development of biogas plants are important factors in the development of sustainable, zero-emission organic agriculture that also realises the objectives of sustainable development and the principles of a sharing economy and a closed loop economy. Biogas plants, including microbial energy plants that generate energy from biogas that can be produced from livestock manure, can play an important role in bringing about a green transformation of agriculture to create sustainable, emission-free, climate- and environment-friendly organic agriculture, including both sustainable, emission-free agriculture based on crop production and emission-free livestock farming. Biogas plants can be set up and built primarily by farmers' cooperatives and microbial gas plants can be built within individual farms. In this way, farms can be self-sufficient in energy. In addition, a positive pro-climate effect results from the combustion of methane, which is particularly strong in terms of generating the greenhouse effect, because even if the CO2 produced by combustion is not bound into certain rock materials and is released into the atmosphere, the greenhouse effect will be much weaker compared to methane. However, a much better pro-climate solution would be not to emit CO2 into the atmosphere, but to bind it to a specific rock form or to inject it deep into the earth's crust in rock layers that will absorb and chemically bind the gas. In view of the above, biogas plants can play an important role in the development of sustainable, emission-free organic agriculture. However, fully climate-friendly biogas plants will be those that do not generate other greenhouse gases when burning methane. Biogas plants can also produce bioethanol, the combustion of which can be a source of energy and has low emissions.
In view of the above, I would like to address the following question to the scientific and research community:
What role can biogas plants play in the development of sustainable, emission-free organic agriculture as an important element in the green transformation of the economy and the decarbonisation of civilisation's economic processes and in counteracting the progressive process of global warming?
What do you think about this topic?
What is your opinion on this subject?
Please respond,
I invite you all to discuss,
Thank you very much,
Best wishes,
Dariusz Prokopowicz
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Biogas can play a significant role in the development of sustainable, emission-free organic agriculture, some of the ways are:
✨Renewable Energy Source: Biogas is a renewable energy source derived from the anaerobic digestion of organic materials such as agricultural residues, crop waste, animal manure, and food waste. It is a versatile form of energy that can be used to generate heat, electricity, and even vehicle fuel. By utilizing biogas as an energy source, organic farms can reduce their reliance on fossil fuels and decrease greenhouse gas emissions.
✨On-Farm Energy Production: Organic farms often generate a significant amount of organic waste, such as crop residues and animal manure. Instead of letting this waste decompose naturally and release methane (a potent greenhouse gas) into the atmosphere, it can be collected and used for biogas production. This not only helps in waste management but also provides a renewable energy source for the farm's operations, reducing the reliance on non-renewable energy.
✨Nutrient Recycling: Biogas production involves the anaerobic digestion of organic materials, which results in the production of biogas and a nutrient-rich byproduct called digestate. Digestate can be used as a biofertilizer, returning valuable nutrients back to the soil. By using digestate, organic farmers can reduce the need for synthetic fertilizers, promote soil health, and close the nutrient cycle on their farms.
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BIOGAS PRODUCTION/BIO-ENERGIES
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January 8, 2023
Dear Benchouk-Rahim,
If you are culturing your organism(s) in a sealed bioreactor, you can try replacing the air (and the O2) from the headspace by pumping nitrogen gas into the vessel. The N2 can be sterilized by pumping it through a sterile filter. Pumping can be maintained at a low flow rate for the duration of the fermentation. The slight positive N2 pressure will prevent O2 from entering the vessel. Don't overpressurize the fermentor; keeping the headspace to a minimum will also help.
I hope this information helps you.
Bill Colonna Iowa State University, Ames, Iowa, USA wcolonna@iastate.edu
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I looked at biomethanation, H2 supplemention and a two-phase system, but I was hoping to look at other technologies if they existed. Any recommendations would be appreciated.
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Dear Rawan,
By "two-phase system", did you mean feeding a photo-bioreactor with the enriched CO2, and then using the yielded microalgal biomass to produce biogas?
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Good morning,
I am proposing home biogas production solutions.
They are containers in very resistant flexible material.
The problem is that, when temperatures drop, bacteria have a hard time doing their job (anaerobic digestion of manure and food waste mixed with water).
The problem is harder in remote rural zones, without electricity, where solar panels cannot be installed.
Second problem: the cost.
There are heating covers, but they are very expensive and the farmers can't spend more money to heat digesters than to buy them.
Do you know any other system to heat digesters or biomass inside?
Thank you all for any replies.
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You can add hot water with feed.
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Does anyone have any case studies on the Environmental Impact of Milk Production?
Environmental Impact of a Dairy is what I studied but unfortunately it is completely different with that of the Milk Production
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Faraed Salman THankyou for sharing both the links and I personally read the shared articles and publications and the second one is quite interesting which shows the environmental impact of the dairy industry which is yes connected to the question raised by me and this is in the circular economy and the part of the question, thank you for your time and the publications you had shared.
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Capacity of plant is around 2-3 m3 . After critical survey on the literature I found there is lack of ideas and suggestions on agitation of slurry inside  such mid-size digester (6-8 feet) .Many of Methods encountered on internet are practically not feasible. I request researchers to suggest for the the same.
Thanking you.
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Rishi Pareek Etiher with the help of Mechanical seal or one of the simple way is to immerse the pipeline much below the slurry level to avoid gas loss from the system.
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Biogas production
Design of biodigester
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You might find it interesting to try formulating two-stage anaerobic digestion process for enhancing biogas productivity. You might follow the attached article for the progress in two-stage anaerobic digestion process.
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Both in small scale digester as well as large scale digester.
For Feedstock 1. Manure only 2.Manure mixed with straw, urine, and grasses.
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Many fungi produce acids but they are not normally a problem in anaerobic digesters specially if there is enough alkalinity available. Often fungal enzymes are more aggressive than many bacterial enzymes and may enhance the first step of methanogenesis (hydrolysis of macro molecules which is the slowest step.. AKA rate determining step)...so they can help instead of inhibit. However,
If pH drops below 7 for any reason (bacterial or fungal) the lower pH causes methanogens to be inhibited or die. If there is a very high increase of bacteria or fungi added suddenly, the hydrolysis step may increase too fast not allowing the next step of methanogenesis to catch up and to metabolize the organic acids produced in the hydrolysis step causing drop in pH.
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Hi,
I would like to ask where I can get a dataset for an Artificial Neural Network I would like to build for the prediction of biogas yield and methane content. Ideally, I will require at least 500 cases.
I have searched many articles on databases, but so far I have been able to download only 1 dataset from the article (Modeling of biogas production from food, fruits and vegetables wastes using artificial neural network (ANN), Goncalves, et al, 2021)
The input data for my ANN will be: OLR (g VS/l.d), HRT, Temperature, pH, Reactor volume and the expected outputs are biogas yield (L/(g VS) and methane content (%).
Can someone please share some information as to where I can get a dataset for my project or if anyone would like to share the data from their experiments, it will be much appreciated.
Regards
W
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Ramón Piloto-Rodríguez YES I am about to publish a paper on this and working towards the neura network dataset for biogas production as well
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what are the most needed devices or measuring device needed in a renewable energy lab. for education and research in
solar energy
biogas
wind energy
others
related to agriculture and agricultural engineering
-----
thanks in advance
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Ashrf Abdel Galil Anwer the experts above have already cited and for solar equipment, one can easily understand the requirements for the laboratory related to solar products and these are available online as well meanwhile there are laboratories in every city for this requirement
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I am starting to run a biogas experiment, but finding problem ordering the best lab equipment as most of what i see on the market is Chinese, and i seem not to trust Chinese stuff! so i need help locating the best apparatus for accurate results as am looking at publishing a paper out of the experiments.
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Charles Sendaaza There are many equipments when we talk about the Biogas laboratories however Hamit Can has cited few in the attached copies and we do also help companies and institutes/projects to install these laboratories especially to work on Biogas/Anaerobic digestion
Srinivas kasulla
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dear all, does anybody knows the typical size and price of an anaerobic digester to be used for local purposes? what of we it would be used in a cattle farm?
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Saba Gholizadeh There are many factors d on which the cost part depends right from the size of the plant, feedstock on daily basis, environment, end use of gas etc etc and to give you an example do we need based on the feedstock capacity or the digester volume.
Suppose we assume 100 cows and they produce approximately 10 kgs of cowdung per head which means the total cowdung available for gas generation is 1000 kgs and this typically has around 12-16% solids so assuming 1:1 water/recircualte/slurry we can go ahead with 2m3 * 25 days + gas volume digester design and this might roughly cost you approximately INR 4-7 lacs for the digester and other equipment and accessories separate.
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Working for a research study on process optimization of anaerobic digestion where food waste is the main constituent.
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Interesting question though we all know the answer but it would be tough to put it in words and yes even I would be waiting for the answer to this question
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Agricultural biogas plants require a certain amount of energy, both electricity and heat, to work and produce biogas. How can I quantify this value? Not considering the energy necessary to convert biogas into electricity (biogas is addressed to upgrading) and considering agricultural substrate (cattle manure, sillage, ... ).
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Anaerobic digestion has two types: mesophilic and thermophilic. Thermophilic uses heat and the amount of input energy is the decision of the operator. If you're using electricity then you can determine from the rated wattage of the heater, or by measuring the current and voltage using meters to determine the electric power consumption. For mesophilic, basically no energy is used unless you count in auxiliary things such as transportation etc., which may vary depending on local conditions.
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Hello,
I just want to know what is the water content of digested cow dung after 30 days of retention time knowing that we add 1:1 water to the fresh dung?
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I hope you are doing great. You mentioned that you are going to add water to fresh cow dung at 1:1 ratio, but the important thing here is that the "fresh cow dung" contains up to 80% water in it, depending on type of the cattle, what it eats, and how the manure is collected from the cattle farm. So that you should initially measure the water content of the dung. Afterwards, by adding more water, you will exactly know the amount of water in your anaerobic digester. If you wanna calculate the remaining water content in your AD digester, you should know the operation temperature of the digestion process at first which good be 10-60 centigrade degrees. Then you can calculate the amount of remaining water using energy balance equations related to sensible and latent heat flows. Another option could be measuring the water content of the produced digestate or slurry, if you have a continuous anaerobic digester. So by having the percentage of the water in the digestate, you could estimate the approximate mass percentage of the remaining water in the digester.
Best
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I am working on Biogas production. The gas is formed. It supports combustion but it doesn't give blue flame that lasts.
How do I make it give a blue flame that is indicative of biomethane?
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The yellow flame many times is due to the restricted air supply in the burner, producing excessive CO. You may try another burner or clean the existing burner and try to adjust it.
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Hi dear researchers, I hope you are doing great. i need a model to predict biogas production from cow and hen manure and also the amount of digestate that remains at the end of anaerobic digestion procedure. i have studied more complicated models such as ADM1 but i am wondering how can i find the amount of the parameters used in this model. does anybody know the amount of these parameters and constants for this two manure types?
does anybody knows any simpler models that predict the amount of digestate as well?
I am looking forward to your responses
regards
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Hi Saba, I hope this email finds you well. AS you mentioned in this area researchers face the three-step models. Firstly, an experimental model that indicates how much biogas produce and digestate reminds at end of the anaerobic digestion procedure that I put some document related to this part, I hope they show u what does happen in this procedure overall. Secondly, u requested some simple models for improving the accuracy of the results. To be honest, I give much credit to these models due to the fact that they are more practical in comparison with complicated models and they integrated easily with other models for predicting the bigger systems. I attached one of these models. Finally, researchers use complicated models such as ADM1 or some equations with non-conventional reaction kinetic forms that have been existed in a lot of software such as Aspen Plus. But I believe they are used for the specific condition that accuracy is very pivotal. BTW, I suggested focusing on the first and second models and for complicated models, you can use equations of other materials such as wastewater or plants. I believe you can investigate these models if it is necessary for your research and developed them for your topic. stay safe and well.
best regards
Danial
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We are going to buy a batch lab-scale biomethane/biogas potential instrument to measure biogas potential of organic waste. What would be your suggestion considering the limited budget of less than $7,000?
I am looking forward to hearing from you
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Dear Amin,
for robust, inexpensive and yet precise BMP determination you can use the Gas Densitiy Method:
You can find additional (and mostly open access) documents on our website on BMP Methods:
If you have specific questions, please let us know.
Best regards and take care,
Sören
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As part of my ongoing project, I am trying to examine the biogas production through anaerobic digestion of vegetable oil. Due to the oil layer formation in the mixture, I doubt that all the bacteria of sludge are getting chance to degrade the oil layer. It might be better for me to use any reganet/ emulsifier to dissolve oil in water. Can anyone suggest a good emulsifier that I can use which won't create any problem for the anaerobic bacteria?
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@Akshay If I use ether or hexane they will add the organic carbon content in the mixture. I was trying to avoid that. Do you know about any inorganic solvent for oil?
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Laboratory Scale Experiments for Biogas Production from degradable solid waste. How can we collect it in an easy way and analyze its qualitative and quantitative properties?
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Doaa Adel Elemam photographs shared are perfect and it depends on the plant size and outlet of the gas specific for me I had collectedin water bottles under immersed conditions and collected gas which was scrubbed naturally as well and also in the bags and bladers (specially something like bladder inside the football or basket ball) and u can rinse with water and dry it and use them for the laboratory purpose
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Could you please share with us your latest scientific achievements (i.e. papers, books, etc) regarding anaerobic digestion technology? Your worthwhile findings would definitely respond to the need for "Engineers without borders" worldwide for tackling the energy crisis, especially in the Global South.
Plaese discuss about your achivements.
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Biogas recovery for sustainable ciities the critical review shared by Mariana Cardoso Chrispim is really good and am sure that might be of some help to you Dr Mohammad Javad Bardi and yes as I said it depends on personal basis and have loads of 20+ years of exp and yes I can share on individual basis whenever we can and would like to exchange ideas
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Hello, I am working on biogas production simulation on Aspen plus. So I have this one stream after hydrolysis in which I would like to change the mass fraction of certain components.
For eg, if the multiplication factor that I want to use is 1.5, then the previous concentration of ethanol after passing through this particular block should be multiplied by 1,5 now. Is this possible on Aspen using calculator blocks
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You have to rationalize mass fraction of components of your interest by hand. Then use the mass fractions in Aspen plus. Aspen can change mass fraction/mole fraction. But not your desired function.
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I'm doing a research on the enhancement of biogas production using grass, chicken manure, cow dung and pig manure.I have attached the bottles with sample to a gasbag. So that afterwards the gasbag can be used to measure the volume of gas produced. I have repeated this experiment 3 times without succes. I have increased the mass and changed the temperature from mesophilic to thermophilic. After 32 days there is still no swelling of the gasbags. However in the lab near the gasbags there is a smell of gas production. 
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Depends on the gas bag weight
simple remove the gas or air from the bag and ensure it hangs on a support and make sure that the gas entrance into the bag is not holded automatically the gas will fill in for sure
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We know HRT of fresh cow manure to produce biogas is more than 25 days(Ambient temperature around 30 degree centigrade) . But biogas can be produced in around 2-3 days during activation period (initial period of bio-digester activation). I installed the system, put fresh cow manure in the system and start to get gas in 2-3 days.
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Bashu Gautam basically the balloon or the bag or the fabrics used for these type of bag digesters are usually quite thin and the temperature is maintained by exposing it to the air/atmosphere at the same time the L/D ratio is completely different in this case and more bacteria gets more surface area and thus the gas enhancement or production is seen more in this case
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Does anyone have data or articles on carbon footprint for a whole lifecycle of a biogas plant? For other parts of lifecycle, i.e. feedstock, biogas production and so on, I can find easily. However, studies which would include also plant construction (and preferably demolition) are not available.
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Thanks Sanna Taskila for posting such an informative question.
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What is the best Artificial Neural Network (ANN) algorithm for biogas production optimization?
Is there a minimum data size for modeling implementation?
What is the best code to this such as SCILAB or Simulink?
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In my experience for the optimal operation of the gassification procces the best model consist in 2 hiddden (4 and 8 nodes, respectively) , input and output layers, sigmoidal tangent functionrs. I am attaching a publication related.
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Any idea how much N can be fixed in an aerated lagoon, assuming the DO is more than 2.0ppm.? Would it be advisable to add Nitosomas and/or Nitorbacter bacteria into the lagoon to increase the N fixation? The intention is like this. Incoming to the effluent pond has COD of 40,000ppm and BOD of 20,000ppm. CNP ratio is 300:5:1 already. I plan to bring down the COD/BOD using anoxic process, that is maintaining the DO at about 1.0ppm in a 10 day HRT lagoon. This will suppress biogas production (GHG). However, this process is a denitrifying process. So CNP ratio will be worse after the anoxic process. In the aeration process to be at optimum we need CNP at 100:5:1. Since aeration is a nitrifying process can I enhance the nitrifying process to bring the CNP nearer to 100:5:1 from the high of >300:5:1? How much N fixation can I attain in the aeration process in 10 days? Would it be enough?
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Andrew Liew yes I had these types of studies along with the test results but not able to search them....once i locate them then surely going to share with you
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How we can choose the best AD pretreatment methods for a given feedstock substrat in terms of biogas production and biodegradability rate and optimizing the costs?
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There are many pre treatments available and particularly it depends on the feedstock/ rawmaterial and local conditions too. There are many factors depends on selection criterion and one has to do some feasibility study before getting or selecting the best that suits a particular feed stock.
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I have used 15kg vegetable waste as substrate in the anaerobic batch reactor with 5 kg cowdung as inoculum and closed it .Even after 30days the biogas is not produced and the pH level is 4 .
how can i increase the pH , even if i increase the pH does biogas production starts ?
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Try adding some more of the Chicken litter or Cow manure whichever is available based on the digester volume and the feed per day to the digester.
Apart from this if you are looking for immediate results try adding Lime Stone powder again you have to calculate and add it in water and then feed it to the digester.
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I made archaeal community analysis for samples from different anaerobic digesters for biogas production and get the results. I can not get any information abut this taxonomy :
d__Archaea; p__Euryarchaeota; c__Thermoplasmata; o__norank; f__norank; g__norank; s__Thermoplasmata archaeon enrichment culture clone A2-24
the relative abundance of this archaeon increased during my studies from 1.10% to 18.98%.
I could not get any information about it's metabolic pathway. could you help me?
thanks
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There isn't any. As the name indicates, it is a sequence obtained from a clone library and therefore no genome is available. The best you can do is finding an identical or extremely similar sequence from a sequenced genome/MAG and go from there.
Check the GTDB, you might be lucky enough.
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The work is on biogas production. Found out that the gas produced extinguishes fire rather than igniting it.
Suggestions too on which substrates that would yield more of methane would be highly appreciated.
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You can try to increase bio-methane yield in a slurry of pre-treated corn wastes and chicken manure with little cow dung and CaO. The bacteria present in the cow dung in alkaline medium may decompose organic matter leading to evolution of methane gas .
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Could you suggest me a solution for generating methanogenic bacteria during the anaerobic digestion process? Shall I add cow manure to produce them? or addition is not necessary?
Thanks.
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I know that faeces contains such microb, but addition of cow dung will facilitate the production of methane.
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Dear all,
is it possible to calculate the amount of material digested in an anaerobic digester, knowing the starting feedstock and the amount of biogas produced?
Thank you in advance
Claudio
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Knowing the daly biogas volume production and CH4% and CO2 % mean values.On using CH4 and CO2 densities, you can then calculate the digested mass by adding m(CH4) and m(CO2) : which are the main biogas content.
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What are your thoughts about the equipment? Also, what was the average cost of the equipment?
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I agree with you that there are many self-appointed "experts" that pretend calculating the BMP of mixtures with tables, or doubling the production by adding "magic powders" of whatever nature. But BMP tests can be used indeed to improve the plant's performance. I developed a method and condensed 10 years of experience in true plant optimization in over 100 plants in Spain and Italy using AMPTS Light , anybody can buy and learn: https://www.routledge.com/Managing-Biogas-Plants-A-Practical-Guide/Rosato/p/book/9781138626614 . Anyway , changing one motor every 2 years or so is unacceptable for most customers, at least in Europe, that's why Bioprocess Control abandoned the DC motors and switched to brushless motors, which are virtually eternal. With brushless motors the stirring is exactly the same in all reactors, because the speed is controlled by an inverter and furthermore you can actually measure the unit power, which is impossible with DC motors. Anyway, as long as there is some stirring, and as long as the inoculum is not overstirred, there's no relevant difference in the BMP. Of course, the final word belongs to the purchase department. Unfortunately, thousands of researchers still do tests with eudiometers, syringes, manometers and similar self-built tools, others shake the bottles manually twice a day, just because their universities cannot afford anything better.
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Which kinds of biomass, byproducts and waste are the most interesting for new research development in anaerobic digestion and gasification?
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From a more generalized viewpoint, the promising kinds of feedstock for renewable fuels should:
  • Exploit energy plants of high heating value (HHV) and low moisture content.
  • Follow low carbon footprints.
  • Be cost-effective, in terms of feedstock transportation from the source to bio-refineries.
  • Satisfy energy demand to local and remote residential areas, having no stable connection to the mainland grid.
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I am planning a new set of AD experiments (agricultural waste as a substrate), and if I run a duplicate of my trials, I will have a total of 6 reactors (this seems a lot of work since I have to combine the experiments with other works), so I would like to know if it is acceptable to run 1 reactor for each condition of my experiment? are there literature where this has been done/justified? Thank you
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Well , I don't think u can loose any of ur reactors in as much as all the operational conditions are in order e.g maintaining ur operating temperature, constant agitation and ur digesters are air tight. Once there is a control, duplicates may not be necessary.
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I am running a reactor for the AD of agricultural waste, and unlike the BMP tests, I understand that the calculation of the biodegradability (efficiency of degradation) of the semi-continuous process is different. I am wondering how best to calculate the biodegradability, considering that I am feeding my reactors daily.
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You (can) measure how much feed you put into your reactor in terms of VS and COD. You (can) also measure how much methane you produce.
Thus you can calculate / know how much methane you produce per gVS or per g COD.
We know that 1 gram of COD can theoretically produce 350 mL methane (or to put it differently: the COD of methane is 64 gram oxygen per mol of methane, which is 22.4 L at standard temperature and pressure). Using this value you can calculate the efficiency with which the COD in the feed is converted into methane.
I am not sure what you are feeding your reactors, but if you additionally measure the concentration of volatile fatty acids (VFAs) in your reactors you can additionally calculate the fraction of the feed that is degraded/converted into VFAs (make sure that you take the VFAs in the feed into account when you do this). Using the theoretical COD of VFAs, you can now additionally calculate the fraction of COD in the feed that is hydrolysed (remember to also take methane into account in this calculation). Indeed, adding the COD of the VFAs formed and the COD of the methane formed, you can calculate the efficiency with which the COD in the substrate is degraded.
And by comparing the methane formed in the system with the methane formed in the BMP tests you evaluate how efficient your continuous system is in terms of methane recovery.
Doing all this you should be aware that some of the methane will leave your system via the water phase, so you should take this methane (production) also into account.
It goes without saying that all measurements and subsequent calculations are ultimately expressed per reactor and day; (feed in on a daily basis, methane and VFAs out on a daily basis).
Hope this helps.
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Respected Researcher,
I want to know about the personal experiences of those who are dealing with Anaerobic Digestion with a focus on "Biogas production".
1: How can we increase the Biogas production from the anaerobic membrane Bioreactors having "Synthetic Wastewater" as feed. The initial COD conc. of the Synthetic Wastewater is around 200 mg/L.
2: What could be a few possible factors overcoming/decreasing Biogas production from those AnMBRs?
Any examples, personal experiences, detailed descriptions/opinions are mostly welcome.
Best,
S.S.A. Shah
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Hi Prem - please reach out to me at jcleary@geosyntec.com and I can advise you from my experience doing projects with organics and food waste. Joe
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What is the relationship between optimal value, in terms of biogas production, and co-digestion ratio for two substrats? Is there any equation defining the relationship between the substarte composition and its optimal value?
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Yes I agree with you. We would spend lot of time the find the optimal C/N ration
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Do you think that future research on this topic will be more oriented to the development of new approaches to biogas and syngas generation? Or the major challenge is rather the treatment of new kinds of feedstock?
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A challenge and an opportunity is to take advantage of the synergies between anaerobic digestion, gasification and Power-to-gas. Heat integration seems to be a first step especially if you need to heat the digester, dry the digestate and/or regenerate the scrubber liquid in case of amine based biogas upgrading. Separated CO2 from AD/upgrading and gasification/methanation as input for Power-to-gas. Use the microbial consortia in the digester to convert nitrogen free syngas from gasification to methane. Lower the specific investment cost in upgrading and downstream processes by combining AD, gasification and Power-to-gas.
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I have waste water from palm oil mill which consists of about 3% dissolved solids only. Organic solids is about 70%. COD/BOD is about 30,000ppm and 15,000ppm. Can I used aeration biological process to bring BOD to 20ppm for discharge? Or do I need to go through the normal anaerobic, facultative and aerobic process to do that?
In the laboratory I have aerated this waste water and brought the COD down to about 3,000ppm within 21 days then it sort of get stagnant there. I have bioaugmentated the samples with facultative microbes to initiate digestion because the samples were sterile from source. People said I need to have the 3 biological processes like that in the effluent pond to bring down COD to 400ppm and BOD to 20ppm. Please provide your professional advise.
I am trying to use aeration to prevent biogas production and at the same time bring down the BOD as fast as possible to bring it to below 20ppm for discharge.
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Yes, you can. But you may want to ensure that you have a low-loaded, well-mixed reactor, to keep the concentrations low at all stages. A plug flow system may have the concentrations so high at the inlet that you will be operating at DO close to zero, and that may result in problems with sludge settleability and foaming.
This scenario is where anaerobic treatment would be considered - maybe UASB followed by an aerobic polishing stage. As others have indicated, you would save money and generate a biogas that could be used for heating around your site, or with a CHP system to generate electricity as well as heat.
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for ADM for biogas production from codegstion/
plz suggest any book so i can easily access software
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I hope the following is your desired
Anaerobic Digestion Processes
Nigel Horan, Abu Zahrim Yaser, Newati Wid
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I would like to ask and discuss.
Have anyone done research on the biogas production with additional of treatment using subcritical water technology? I have difficulties and confusion, as when I did preliminary experiments towards the treated and untreated material, according to hyphothesis should be the treated material produced the highest biogas production. However, the actual results were the opposite.
Did anyone encounter this type of findings?
Thank you.
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Hi @Nadia
It is true what Dr. @Bachmann mentioned that it is better to do carbon balance. Some parts of the substrate carbon may have been decomposed to gaseous or volatile compounds during heating.
In addition, the heating process may produce compounds that are toxic to microbial communities involved in AD process. So, it may be better if you do analysis about these toxins as well in the substrate.
Regards,
Alam
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Process parameter optimization for biogas production from lignin via Anaerobic Digestion is a lengthy process when DoE is applied to full scale processes - is there a possibility that laboratory scale experiments, over a much shorter period, could be conducted instead?
It would be appreciated if some guidance could be provided, and/or a direction to information or literature.
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The enquiry relates to small scale laboratory experiments to set baseline individual parameters over a short period of time - for a novel feedstock. These parameters will then be applied to full scale trials, and optimized for the process.
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There is definitely energy production from all species waste. What kind of energy can we produce? Can it be used to sustain a building? How would we collect and process the waste? 
 I would really appreciate if someone shared their expertise!
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Most researches report a hydraulic time of 30 days and above in biogas production. Are there possible ways to reduce the HRT and also obtain more gas?
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Dear Kelechi Ukaegbu-Obi ,
the degradation of organic compound in anaerobic conditions is dependent on degree of polymerization, thus its time may very e.g. cellulose is polymer of sugars ( polysaccharides ) takes a longer path to be degarde or breakdown needs higher HRT. where as simple sugar would take shorter path to decompose in to CO2, H2O.needs shorter HRT.
yes you can reduce the HRT if some enzyme like Cellulase (for above mentioned case) is added to the system in hydrolysing stage
I would recommend that please get the analysis & identification of compounds present in wastewater, then find decomposing accelerator (enzyme etc) for the same to reduce the HRT.
regards
Dinkar Saxena
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I have a question about biomass: in new Caledonia it could be possible to use biomass for biogas production, but because of the particular soil rich in nickel ores, the biomass contains high concentration of such metal. If the digestate was used as fertilizer, the soil used for agriculture would be heavily contaminated. Do you know if the biomass can be treated in advance for reduction of Ni and, if so, what technology can be used?
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Since the biomass carries such a lot amount of metal, a treatment using acid might be suitable for leaching all metal content from cell.
A further treatment to the metal containing acid can be carried out using ph adjustment to achieve metal separation.
This method might be expensive to be used in a large amount of biomass. But i think this is the most proper way to be applied, based on my limited knowledge.
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in our research, we reduce the lag phase of biogas generation(4-6 hours) and reached very early biogas production at high yield in a batch system (HRT=19 hours). how can I identify the microbial variety of inoculum? what do you consider as an important factor responsible for such early and high biogas production (y=460 ml/g vs)?
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Dear Javad,
The increase in biogas in early stage depends on the method you applied for reducing the lag phase such as pretreatment. For finding the microbial community you can take samples from early stage and perform High throughput sequencing (HTS) to see abundance at phyla, class and genus level. There may be some increase in hydrolysis stage in your case which can be considered as an important factor for lag phase reduction and high biogas yield.
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Hello RGians,
In optimization studies of biogas production, I could see pH and TS have used as independent parameters.
In actual practice, is it possible to set pH and TS of feed to required value?
I think adjustments in one parameter also changes other parameter (in case of TS and pH).
Could you please explain the practical method of fixing pH and TS as per a an experimental design?
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Dear Karun,
I suggest you to see links and attached file on subject.
- (PDF) STUDY ON THE EFFECT OF pH ON BIOGAS PRODUCTION ...
- Small-scale biogas production in the province of ... - DiVA portal
- PRELIMINARY STUDY ON BIOGAS PRODUCTION OF BIOGAS ...
- Biogas Production from Kitchen Waste - ethesis - NIT Rourkela
- Biogas Digester
Best regards
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What should be the ratio of co-substrate dilution for the preparation of slurry to get optimum results for biogas production? What other diluting agents can be used in place of water or urine?
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Your question implies that you need some basics of anaerobic digestion and biogas processes. Please read this.
Nevertheless, there is no general rule, and there are a lot of parameters to consider to decide the feed ratio. By the way di you have anything which is cheaper than water for diluting the feed in bioreactors?
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Slurry left after the production of biogas still having some of the heavy metals in it. Should it be dumped ?
Will it affect the soil again? Can it be utilized as Fertilizer?
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The weed biomass can be used for bio-remediation and bio-adsorption of metals and pollutants; biogas and biofuel production, composting and vermicomposting, as feed for animals and fish, as carbon source for microbial growth , and for various medicinal and other uses.. The slurry or sludge left after biogas production is usually transported and used as liquid fertilizer. For details consult https://scialert.net --fulltextmobile
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What will be range of fresh cow dung COD in term of biogas production from these substrate.
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Thanks Sir,
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Im chemical enginI am a chemical engineer and my research project is the production of biogas with whey. And my work is in the first phase a proyect i work is biogas production whit lacto serum and the proyect is in a first phase
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Dear Jorge
Based on my experience, I recommend you the use of the EGSB bioreactor (third generation bioreactor), which will allow you to exploit the full potential of the chees whey (lactoserum). Since this bioreactor, being an anaerobic bioreactor of upflow with a high granual bed expansion, it will allow you to generate biogas rich in methane (concentrations above 60%), the only thing you have to be careful of is in your control parameters such as pH and the alkalinity factor; due the serum to be a substrate rich in proteins (TKN), which mostly generate a C / N ratio less than 15/1 which promotes the generation of ammonia, alkalizing the system, so for this problema, I recommend buffering with NaHCO3 to have a higher performance in addition to that you have to adjust the pH near 7 before to feed.
Below some articles are attach:
pd. if you require granular anaerobic inoculum for EGSB adapted to chees whey, we have.
Best regards
Abumalé
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I am currently carrying out a research project on biogas production and using a GC Agilent 7820A to test for gases.
I am using a cappillary column GasPro 30m * 0.32mm and it is not giving me satisfactory results, and there is also an issue of large retention times accompanied by this column. With your expertise, please assist me with this predicament at hand on the choice of a column suitable for gas characterisation. From the inforrmation i gathered, the use of molecular sieve Poropak Q 2m* 2mm column(or in that close range) would give me better results.
i would also apprciate if you could further advice on this decision i am making.
Your assistance and cooperation will be greatly appreciated.
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Thank you
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When producing biogas from a semi-solid or liquid substrate, water is added to dilute it. The slurry dilution factor actively affect the biogas production, as higher water content may dilute the decomposer cells and enzymes per unit substrate, which is bad for the process .
So, my question is that what is the best slurry dilution factor for biogas production anaerobically using cattle manure, where I can achieve maximum biogas yield?
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Dear Pankay,
Indeed, if use only cow dung (TS is ca. 15-16%), the ratio is ca. 1:1 so as have a slurry humidity of ca. 90-92%. If use co-digestion with other organic materials, the water to be added should be calculated considering the TS of each material, then determine the average TS of the organic substrate to get a mixture of ca. 8% TS. For example, if use a mixture of 8 parts cow dung (TS 15%) and 2 parts grains straw (TS ca.86%), the average TS of the mixture will be (8*15 + 2*86)/10 = 29.2%. For bringing the mixture to 8% TS, the water to be added is determined as: 10 parts organic substrate (cow + straw) * 29.2/8 = 36.5 parts diluted material. This means that you should mix 10 parts organic substrate and 26.5 parts water. And so on for any other mixture...
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Do we use fresh sludge from the another digester that is in the same place to fill? Does the active sludge get ready for another refinery?
Do we use animal manure or another combination by active sludge for the initial loading of 6000 digester? If yes, how many percent of cow manure and a few percent activated sludge?
How to fill the 6000 m3 digester first time? When we fill the digester, how much time should we consider the initial sludge for the first time?
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In this case if the source digester is far from the sink digester, you can use sludge trucks to transfer the inoculum sludge. You can discuss your particular case for better suggestions.
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I understand its difficult to ferment Lemongrass without treating it. What are the available methods to pre-treat in 3days
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Thank you Sir
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I'm a student in the final year of my M.Sc in Environmental Engineering. I want to work on a project focused on the feasibility of using the combined advanced oxidation and biological processes to treat highly contaminated petroleum refinery wastewater.
I want to ask you to borrow me about 10 minutes of your time and answer my question. Is anyone able to help me with this?
What topic can I use for my thesis?
Any prospective leads and suggestions would be highly appreciated.
Thank you.
Saeed Molaei
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Dear Saeed,
This is one of the main concerns for students who must determine their own research topic. So, please do not be frustrated. I know that you are looking for a new, yet efficient, way to refine one of the most complex sewage. But please note that your time is only one year, and this time is not enough to carry out the number of tests. My suggestion is to combine two of them, such as oxidation and biological methods, and then focus only on them and all the effective parameters. I can tell without any information and only on the basis of my experience that there are still thousands of experiments that can be done on their combination that nobody has done yet. So you do not have to create a new method, but just do experiments that others have not done. I'll give you a simple example. Changes in temperature, pH, flow rate and feed concentration! That is, even if another researcher has checked the parameter effect, you can change the parameter check range. This is also a new experiment. Please do not drown yourself in a world of papers and methods of purification. What I've done before.
Good Luck
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what are the suggestions for a research topic in that field.
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Dear Acheles Karungi,
The best way to arrive this answer is to conduct a thorough literature review on the subject. As always the best way to begin is search for current published data on the subject using the web of science website.
Next, you can download the top 25 to 50 papers and summarize their findings. Hopefully you will be able to come up with a research gap, questions and hypothesis on the subject.
Best of luck,
BBN
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Before start a new project I need to have a preliminary, basic view on a mixture of potatoes and sugar beet to be used as a substrate for anaerobic digestion. Does anybody has any experience or research papers on this topic? Also, I need to know which kind of Nitrogen-rich co-substrate is most suitable to adjust the C:N ratio and which type of inoculum (manure or fermented slurry). Thank you.
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Maybe this paper could also add some some suitable information.
Best regards
Vit
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Dear all,
If fungi/mold containing grain (let's say barley grain) were about to be digested, how would fungi/mold affect digestion performance? Will it inhibit or will it stimulate the digestion at full scale?
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how many full-scale anaerobic digesters for biogas production and wastewater treatment are there in the world (continent or country wise)?
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I can indicate the number of full-scale AD in Europe. The most recent statistic for Europe is presented by European Biogas Association, that declares a number of 17,358 biogas plants in Europe at 31/12/2015 and a total installed capacity 8728 MW. Presently the total number should be slightly over 18 thousands.
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Looking for a collaborative hand to do large scale Biogas production from Sargassum (Brown Seaweed). I have identified and isolated Biogas from Sargassum long before. I can send you ppt and the video for your reference. After the anaerobic digestion the liquid extract can further fortified with lactobacillus to use as a liquid Biofertilizer.
So please i kindly request you to consider my request and hope you will do me the best.
We are a Seaweed company working abundantly with Seaweeds like Kappaphycus, Sargassum, Gracillaria (Salicornia). So i would like to use this Biomass for producing Biofuels - Economic importance for future.
Looking forward to hear from you.
Regards
Srinivasan.S
Microbiologist
Aquagri Processing Pvt. Ltd
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Here are a couple of their papers. There are probably more that I don't have copies of.
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Iron oxide nanoparticles can be added as an additive (as a nutrient) in anaerobic digestion to enhance biogas and methane production.. My question is: whether the nanoparticles will be added at the start of the process or during the process?
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There are lot of enzymes and different methodologies were used to treat the micro-algal biomass to improve biogas production. However, we need which enzymes and methodology gives good results at laboratory.
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Thermal hydrolysis or use of ultrasonic will be helpful.
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Both Fe2O3 and Fe3O4 release iron ions in digester. But Fe3O4 is used more than Fe2O3. What is the reason behind this?
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Fe3O4 has both Fe+2 and Fe+3 in its structure. They might have better role in interspecies electron transfer because this is what it is mostly used for in AD. As Philip Antwi has mentioned, you should focus on this process for more understanding.
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For lab scale batch study, water displacement method was utilised to measure biogas production per day. But how do I measure biogas production per day in a pilot scale continuous reactor ??
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Thank you
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What are the efficient, cost effective, highly stable catalysts are used in Catalytic Liquefaction to produce Bio-Oil?
Is Bio-Oil is suitable for biogas production by anaerobic digestion?
How to recover catalysts used in Catalytic Liquefaction process? How much is the recovery in per cent?
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Dear Sir,
Thank you for info. Will this bio-oil help to reduce HRT of AD? I have tried to find out the literature on bio-oil to biogas. very few attempts are there for his. Most of the researchers have suggested to go for replacement of furnace oil. Being a microbiologist, I am worried for effect of toxicants and their concentration after conversion into organic soluble form. For eg. lignin will release phenolic compounds . This may lead to reduce the rate of biogas production.
If HRT is reduced after catalytic liquefaction, the growth rate of methanogens and acetogens should be considered since they are slow growers. I had a discussion with one expert in this field. He suggested to go for 2 days HRT. But my worry is it should not wash out the culture. the rate of acid production from bio-oil and conversion of the same into methane is also important to evaluate.
Request to researchers in this segment, please update on this
Thank you
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This equation is used for assessing the biogas production by anaerobic digestion.. Kindly help me... 
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hello, you may refer to these publications. an extensive discussion and how to employ the modified Gomperts and first order model for process kinetics evaluation was presented. http://www.sciencedirect.com/science/article/pii/S0964830516309040             http://www.sciencedirect.com/science/article/pii/S0960852417306326
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Assuming external heating source is not desired, and the digester is made of high density plastic material, located above the ground.
what method should be adopted in temperate and tropical region respectively?
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When biodigesters operate, they emit heat from the bioreactions that take place and since the reactor walls are made of high-density plastic material say high-density polyethylene (HDPE), you could use a coil around the tank walls with water flowing through the coils. Depending on the desired temperature, water flow rate through the coil could vary to keep the set temperature inside the biodigester.
Hope this helps answer your question.
Professor Yehia Khalil, Yale University, USA
Fellow of the University of Oxford, UK
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I need some statistical informations about how often Salmonella spp. occurs in post fermentation residues in german biogas plants. Reffering to European Biogas Association in 2015 there were almost 11,000 biogas plants working. According to european legislation post fermentation biomass have to be examined if contains any Salmonella. How often Salmonella is isolated from post fermentation residues?
I will be gratefull for any publications.
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Anaerobic digestion by itself is not regarded as acceptable for producing a pathogen-free sludge. In US terms it would be, I think, a Class B sludge - public exposure must be prevented for a period of several months.
Most places are looking for Class A sludges, so anaerobic digestion is usually followed by dewatering and cake storage to provide further pathogen dieoff; or to use additional treatment stages, such as higher temperatures.
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The hydrolysis phase of the biogas production process includes the degradation steps of macromolecules to the individual components and finally to fatty acids, CO 2 and H 2 , but also various intermediates. In these degradation processes, a variety of microorganisms is involved or the degradation takes many steps or reactions.
The products of the hydrolysis phase are a substrate for the methane formation by methanogenic bacteria or archaea. For scientific investigations of a double-stage biogas process, which focused on methanation stage, is it possible to prepare a substrate in the lab instead of operating a hydrolysis stage? 
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Im not sure what do you mean with "alternative sources" because it is not clear "alternative to what". I suggest you start whith what you have nearby: cattle manure, food waste, ...? Or if you can clarify your question maybe we can suggest acordingly.
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I will appreciate ideas/ suggestions on construction of a good lab anaerobic digester for effective biogas production and measurement, microbial analysis and physicochemical analysis. Also the type of material to use for it. We don't have funding and we are working in a very remote area. Thanks
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I guess, it is for domestic sewage or farm waste. Latter will produce excellent biogas. I would suggest UASB reactor, since operation and maintenance is simple and it produces gas at a good rate.
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Lots of work have been done using different substrates in biogas product ion and optimization. Are there still new things to research in this area to help in turning waste to wealth and energy and also save the globe from serious implications of climatic change as regards to methane production. Thanks
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Tell me if I am wrong.  Anaerobic digestion is essentially a self-limiting process.  In AD the microbes grow and give off carbon dioxide and methane.  However, in this process the natural life cycle comes into play.  Microbes begin to die.  In the decomposition of these microbes, ammonia and hydrogen sulfide are released.  These are toxic to the new microbes.  Eventually, the process reaches its limit.  Work needs to be done on how to remove the dead microbes from the process so that it can utilize all the biomass available to it.
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Hello guys
I m trying to do species transport to my problem. I chose mixture-template for my reaction and also I defined my needed material. But in the mixture-template dialogue box when I am trying to add my material and remove N2, O2 and H2O (the default ones), I cannot remove them (I can add) and it shows me this error: "Species n2 is used in one or more reactions." for example when I m trying to remove n2 from my mixture. If someone have any experience plz help me.
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Hello there, I don't know what is it that you want to simulate, but in general, the added species by default can be disabled simple by not using them. Meaning that, in your boundary conditions, for example the mass or velocity inlet, you put "O" mass fraction value for the species that you don't use (under species tap).
But if that does not work for you, you have the option to delete any material (under "materials" setup). But first this material has to be totally not used in fluent otherwise you will have error message!
In materials you have three types: mixture; fluid;solid. If you have air for example (under fluid) and you want to delete it you have to disable first all the boundary conditions that uses air, such as inlet, outlet and interior fluid by turning them to something else like "wall" for example. And you have to add another fluid alternative to replace air then you can delete air from your "materials" setup and then put your boundary conditions back to normal.
Under Materials /mixture, you have another option to add your special mixture rather than import it from fluent data-base. You can either read a "pdf" file with your species (file/read/PDF) if you have previously created and saved one. Or you can create one. You have to change first from species transport to non-premixed or partially- premixed model and create and write your pdf file even with secondary stream if you need. Then switch back to the species transport, and the pdf with your species will be automatically in your materials (under mixture).
Hopefully you find this answer helpful.          
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Hi,
I am doing batch experiments for certain substrates to evaluate the effect of various parameters such as co substrate concentration, total solids, temperature on bio gas production through optimisation of parameters.
Can inoculum concentration affects cumulative biogas yield or not?
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Thank you sir.
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I am trying to see the best way to design a mini Biogas system for domestic use in a Rural context, any advice(feedstock: organic waste & sewage etc.)
the issue idea is to have
-few moving parts as possible
-decent yield
-no artificial source of heat 
-size of digester about 50 to 100 litres (just ti have a small footprint) 
 if possible a chat to evaluate various feed stocks (human waste etc)
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Two main type of biogas plants are used worldwide which are fixed dome biogas plants and floating dome biogas plant. Both types can be used for rural application. In fixed dome biogas plant there are no moving parts whereas floating dome biogas plants comprises of moving parts. Yield of biogas depends on the size of the digester and the amount of organic waste. Currently people are using cow dung, kitchen waste, fruit and vegetable waste in these types of digester.
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Since chicken manure is rich in nitrogen so high concentration of manure can lead to ammonia toxicity. 
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I worked on biogas production from cow manure and for that, i didn't find any proper manure to water ratio. As per the literature review, i found that people took 50:50 ratio as a rule of thumb without any logic. I did a batch experiment with different ratios like 10:90, 20:80, 30:70, 40:60, 50:50, 60:40 and so on and discovered 30:70 to be the best mixing ratio. You can also do this simple experiment for chicken manure.
Good Luck.
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for using sawdust as substrate for biogas production
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my kind thanks to all.I used  sulphuric acid pretreatment with the concentration of 1.5%(w/v) for rice straw,rice husk and wood dust.the other conditions are ,temperature 120c(autoclave),and time period of 30 minutes.but i got more lignin(acid soluble) from that pretreatment than two step hydrolysis (72% and 4%h2so4-lab protocol) for the same sample is that possible?
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To calculate the COD removed,
We have the following equoition according to K. N. Nwaigwe and C. Enweremadu
% COD = (COD (O) -COD (t)) / COD (0). (1)
Where COD (0) is the initial COD or the COD at t = 0, its numerical value; While COD (t) = the COD at any time t.
During anaerobic digestion there is an increase the dissolved COD. In this case how to calculated the removed COD?
According to this equation at some time the removed COD will be negative.
Also at the end of anaerobic digestion there is not a large difference between the initial and final COD.
And the great COD values generally recorded in the hydrolysis and acidogenesis phase.
Thank you dear colleague for your collaboration
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Dear Mr. Mohammed,
I will go in the same line as Mr. Allen. It is better to measure total COD (soluble and particulate COD). This will ensure that you don't end up with a negative value. If you measure dissolved COD (also known as soluble COD or SCOD), then you will definitely end up with a negative value at some point due to hydrolysis which breaks down and solubilises organic matter.
If your anaerobic digestion process is working effectively, you should be getting a reduction in COD values (make sure you measure total COD for both initial and final). Besides, it may be that COD of inoculum is interfering with your COD values. You may measure the initial COD as that of the mix of substrates and inoculum and the final COD as that of the whole digestate (both solid and liquid part).
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oil press, biogas plant
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Hi,
Korenzo,
I appreciate your article entitled "PARAMETERS OF BIOGAS POWER PLANT". Unfortunately, the paper did not provide information on screw expeller.
O.D. SAMUEL, Ph.D
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I am interested in the constraints of anaerobic digestion of the biodegradable fraction of MSW for biogas generation at the laboratory level.
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Dear Ashutosh
For some basic insights you may kindly have a look at this article:
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Food in general, especially fresh food, has a high bio-methane potential based on it's organic components. Fast-food has several additives that are not present in fresh food. So what can make fast-food a viable source of biogas?
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Due to the lack of synergy of microorganisms in batch reactors, the biogas produced might not be sustained. As the the anaerobic process progresses through the steps involved, the microorganisms in-charge of the previous stages die out to give way for the microorganisms in the following stages. Other than making a new setup that is not a batch reactor, are there ways to increase efficiency of the batch reactor.
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Higher operational temperature improve the specific biogas yield, but the
qualities of biogas produced may be different.
Please, see following publications for more details:
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The presence of high calcium ions in bambara nut chaff has been regarded as the possible cause of the instability of the biomass in biogas production. 
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Subjecting the biomass samples to pretreatment will be your best option. If the biomass is subjected to acidic pretreatment, the acidic solution reacts with the calcium content of the biomass resulting in some salts and then the pretreated biomass can be washed with distilled water until the reaching neutral pH. That's one path you may want to check out. 
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Different kinds of substrates produce different amounts of biogas and have different rates of degradation. They know some method to determine these rates?
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MÉTODOS SUGERIDOS: EMBRAPA Concórdia.
Sólidos Totais (ST): secagem a 105 °C até massa constante.
Sólidos Voláteis (SV): diferença dentre sólidos totais e sólidos fixos (SF) após calcinação a 550 °C até massa constante.
Produção Específica de Biogás (PEB) e Produção Específica de Metano (PEM): ensaio cinético anaeróbio mesofílico, expresso em volume normalizado de gás em relação a massa de sólidos voláteis da amostra (com base nas normas DIN 38414-8, VDI 4630, ISO 11734 e/ou ASTM E2170-01).
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Reactor size: 320 ml glass bottle.
Substrates: Wheat straw, rice straw, bagasse (Not a mixture, but digested separately)
Temperature: 37 oC
HRT: 45-60 days
pH that i want to maintain is 7-7.5.
Please provide references for the dosage so that i can use it in my study.
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To adjust the C/N ratio of your substrates, you will first need to determine the C/N ratio of the substrates. Lignocelluloses like wheat straw, rice straw and bagasse can have C/N ratios higher than 60:1 (some even above 100:1). Depending on the C:N ratio of your substrates, you calculate how much N you need to add to bring the C:N ratio to the required value (25:1-30:1) for effective digestion. Based on this and the ratio of nitrogen in NH4Cl (14/53.5=0.262), you then calculate how much NH4Cl you need to add... (Amount of NH4Cl=Amount of Nitrogen/0.262)...
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In the application of polyvinylidene fluoride (PVDF) membrane bioreactor in a large scale of biogas production, the membrane layer can be inserted in the middle of the reactor that separates between acid-forming and methane-forming process. In the application of continuous reactor using the substrate that has hydrophobic toxic compounds, hydrophobic substrate theoretically cannot diffuse through the membrane layer. Therefore, the toxic compounds in the substrate will be accumulated outside the membrane layer (acid-forming process), which can inhibit hydrolysis process of the substrate.
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I suggest that you use a scrubbing process (say with water) to remove the toxic chemicals prior to entering the membrane separation process. Alternatively, you could use adsorption process on charcoal prior to entering the membrane separation unit.
I teach this same subject in my core chemical engineering course on Separation and Purification at Yale University.
Hope this helps answer your question.
Professor Yehia Khalil
Yale University
USA
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Dear everyone,
Would temperature reduction in anaerobic digestion reduce the H2S levels IN BIOGAS?
Thank you,
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Yes, according to Delgado et al. (1999) temperature influences the H2S in the biogas:
H2S GENERATION IN A RECLAIMED URBAN
WASTEWATER PIPE. CASE STUDY: TENERIFE (SPAIN)
Wat. Res. Vol. 33, No. 2, pp. 539±547, 1999
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Hi everyone.
I am planning to run a pilot scale anaerobic digestion (Biogas)plant-500 liters which needs to be inoculated with fresh cow dung. Should i add total quantity of cow dung at once or should i add daily some fraction of inoculum to plant until it reaches to full working volume . Because i am thinking adding at once may create overloading that may result plant upset. Please post your valuable suggestions.
Thank you...
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Thank you sir
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I wanted to correlate the experimental solubility data using PR EoS. Anyone please the me the procedure. I am bit confused here. Looking for a clear cut answer for finishing it. Thank you. 
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Good new !
If you have problems to use the tool by Marcelo, please let me know. I will help you.
Jean-Noël.
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Hello.
I'm seeking a cheap and efficient H2S filter with a long life cycle (long time running before changing).
My daily biogas production is between 0.01 and 0.06 m3. I haven't measured the H2S content but It must be in average (1000ppm or less).
I want use the biogas for cooking, nevertheless I worry about corrosion and how do the stove connection.
If someone has a suggestion, I am pleased to hear it.
Thank you.
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Hi. Is it realy necessary?
We have some guys here with biodigesters producing between 1 to 2 cubic meters of biogas per day and they dont have much problem with corrosion. They use brass aloy stoves that dont corrode easily though. But generally I think the H2S is not so much to warrant the need for a desulpherizer even though you could easily make one yourself using iron fillings or steel wool.
Goodluck and maybe share with us what you come up with
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Is there anyone to support our project Bio gas from brown seaweed ... (Looking for a partner or a collaborative agent or a financial support)? 
Bio gas from Brown Seaweed....
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Dr. Parthiban,
Many of the organisms can produce but the creative of the project is based on the availability of the substrate input. We are having abundant source of brown seaweed so we were trying with this particular sp. and is a dual benefit overview (Bio gas and a liquid seaweed fertilizer).  
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Hi. I want to use fresh cow dung as inoculum. What i have to do if i want to use cow dung as substrate also. Should i have to dry it for conversion of fresh cow dung( Inoculum) to substrate?
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Hi
What is the volume of the digester?
What are the objective of the project? Are you doing a research? Besides cow dung, do you have any other substrate?
The reason for those questions is mainly that, if you are doing research, you can always have cow dung as the inoculum but could do a separate batch reactor that uses only the cow dung and this will be the "control" upon which the other batches can be compared.
If you are producing biogas for actual usage at a household then you can use cow dung as the substrate without drying. So, my understanding of your context will help in giving you the proper advice.
Good luck
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Hi.I am measuring bio gas production. I want to know the reason why some authors used acidified water to measure volume of biogas production in anaerobic digestion.
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Because the carbon dioxide will dissolve readily in water which in turn reduces the pH of the water. If the pH is already low, the solubility of CO2 decreases and you can more accurately measure the total biogas volume with the water displacement method. Alternatively, some researchers use a strong basic solution to completely strip the CO2 and only measure methane volume. 
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In centralized municipal wastewater treatment plants, some recommend to use biological desulfurization with packing media for better biogas production. I wonder how it works, what is the process and operational steps, and more important, what kind of operational problems may occur?
For example, should we change packing media or is there any sediment remain? should we use any chemicals for pH control ?
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Dear Shervin,,
The attached article may  helpful
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I want to do a research in biogas production from lignocellulosic materials (Wheat straw, rice straw, saw dust). My idea is to use alkali or acid pretreatment and than use iron oxide nanoparticles to observe their effect on methane production.. Is this possible? 
You can help me by suggesting more innovative and useful idea about biogas optimization but the idea should be related to nanoparticles.
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Yes,. You can do that. I think you should also focus on  pretreatment results before digestions. This  will give you more information about the availability of your cellulose before AD. Thanks 
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we are doing the biogas experiments using micro algae. 1kg of Scenedesmus obliquus micro algae gives how much biogas especially methane theoretically and practically. Co digestion is require or not for micro algae based biogas production.  
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The theoretical methane potential for Scenedesmus obliquus, based on its gross composition is 0.59-0.69 L CH4 g VS−1 (Sialve et al. 2009, see attached file).
The actual methane production is much lower (0.18 L CH4 g VS−1) and pretreatment may be useful to increase this production (Gonzalez-Fernandez et al., 2012)