University of Glasgow

Nanjing Tech University

Analytical and Bioanalytical Chemistry is an international journal dealing with all aspects of the analytical and bioanalytical sciences. The journal covers all fields of pure and applied analytical chemistry and bioanalysis, including topics at their interfaces with the life and health sciences, the engineering and materials sciences, environmental science, the earth sciences, and others. Fields covered include chemical, biochemical, biological, and physical methods of analysis (e.g. sensors, bioassays, spectroscopic techniques, chromatography, electrochemistry), instrumentation, sampling and sample preparation, separations, miniaturized systems, chemometrics, quality assurance, method validation and process control. The increasing need to develop fast, reliable and environmentally friendly analytical procedures or even complete analytical strategies within a very narrow time frame is a task which needs - more than ever before - the extended interdisciplinary cooperation between the experts in the fields. Therefore, Analytical and Bioanalytical Chemistry has a pronounced interdisciplinary and practice-oriented character and is also devoted to the development of strategies for problem solving in analytical and bioanalytical chemistry and aims at fostering the cooperation between all relevant disciplines.

Analytical and Bioanalytical Chemistry

ω-Transaminases (ω-TAs) are widely available for the production of chiral amines and unnatural amino acids. Herein, a rapid spectrophotometric method was developed for screening ω-TAs based on the colored products that can be generated from transamination reactions between aliphatic α-diketones and amino donors catalyzed by ω-TAs. The possible mechanism of the formation of the colored product was investigated according to LC-Q-TOF–MS analysis. Among seven diketones, 2,3-butanedione was selected as the most suitable amino acceptor for colorimetric screening of ω-TAs with high efficiency, high sensitivity, and low background interference. Meanwhile, the absorbance of the colored product generated by 2,3-butanedione catalyzed by ω-TAs in this method was linearly correlated with the results by HPLC analysis. This method was also confirmed to effectively screen ω-TA mutants with high activity towards isopropylamine.Graphical Abstract

The rapid high-throughput screening of ω-transaminases via a colorimetric method using aliphatic α-diketones as amino acceptors

Identification and characterization of a novel thermostable transaminase (TATP) from Thermorudis peleae

Springer Nature

Chiral amines are vital building blocks and have been widely applied in pharmaceutical and fine chemical industries (Wang et al. 2023). Of late, biosynthetic methods have attracted growing attention for manufacturing chiral amines via biocatalysts instead of chemocatalytic methods, such as transaminases, amine oxidases, and amine dehydrogenases (Tang et al. 2023). Transaminases are pyridoxal-5 0 -phosphate (PLP)dependent enzymes that can catalyze the transfer of an amine group between a donor amine molecule and a carbonyl acceptor, leading to asymmetric synthesis of chiral amines (Nugent and El-Shazly 2010;Ghislieri and Turner 2014;Schrittwieser et al. 2015;Slabu et al. 2017;Yin et al. 2020). 

Zhengheng Zheng's research while affiliated with Nanjing Tech University and other places

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