Yong-Guang Jiang's research while affiliated with Capital Medical University and other places

... In particular, PTTG1 is one of the 17 gene signatures that can predict metastasis and prognosis in various tumor types (Ramaswamy et al., 2003). Some previous experiment-based studies have also indicated that PTTG1 is involved in various tumor processes, such as growth and metastasis, by revealing that PTTG1 overexpression is linked to proliferation and invasiveness (Zhang et al., 2014;Yan et al., 2015;Lin et al., 2016;Meng et al., 2020). Interestingly, the downregulation or silencing of PTTG1 could lead to the opposite result (Caporali et al., 2012;Cui et al., 2020;Meng et al., 2020;Zhou et al., 2020). ...

... The incidence of hypospadias is related to the type of congenital heart disease, and both may have common pathogenic factors. 10 On observation of surgery, urethrocutaneous fistula in 7% cases, 9% meatal stenosis complications and 2% wound dehiscence ( ...

... Chen et al. showed that the anticancer effects of melatonin on osteosarcoma OA MG-63 cells were mediated by the inhibition of EMT through the HIF-1α/Snail/MMP 9 signaling pathways [103]. Prostate cancer stem cells with a mesenchymal phenotype are triggered by cancer-associated fibroblasts (CAFs) by HIF-1α/β-catenin-dependent signaling pathways [104]. The expression and secretion of midkine (MDK) protein in lung cancer cells is regulated by HIF-1α. ...

... β-catenin RNA interference and stable cell lines. The recombinant plasmid pSUPER-β-catenin-shRNA was previously constructed (17). It was transfected into SW1736 cells using Lipofectamine 2000 (Thermo Fisher Scientific, Inc.). ...

... In previous studies, we have isolated three types of cell population with stem cell-like associated markers, self-renewal ability and typical holoclonal growth patterns based on a FACS analysis approach (Luo et al., 2012;2013) (Fig. 1). The first was named LNCaP/SP1, which was derived from the LNCaP cell-line, the second was designated as LNCaP/SP2, and was sorted from subcutaneous tumor tissues produced by LNCaP/SP1 in nude mice, and the third was named LNCaP/SP3, which was separated from subcutaneous tumor tissues produced by LNCaP/ SP2. ...

... CSCs also mediate tumor resistance to common treatments such as chemotherapy and radiation (Bao et al., 2006;Li et al., 2008;Bentivegna et al., 2010;Ho et al., 2012;Madjd et al., 2013). In a large number of studies, CSCs have been isolated from patient specimens, cancer cell lines and xenografts using fluorescence-activated cell sorting (FACS) and with a combination of suitable cell surface markers, cytokeratin markers, drug transporters and side population (SP) in Hoechst 33342 staining (Yang and Chang, 2008;Su et al., 2010;Ho et al., 2012;Luo et al., 2012;Zhang et al., 2012). There are functionally distinct subpopulations of bladder CSCs which require further investigation to identify their cells of origin and self-renewal and differentiation features (Chan et al., 2010;Ho et al., 2012). ...

... Patients' nonadherence to medical treatment of male LUTS represents a significant problem [Nichol et al. 2009]. Combination therapies seem to result in better compliance compared with monotherapies [Lin et al. 2012;Nichol et al. 2009]. Less-frequent dosage enhances adherence and technical adherence interventions are usually directed at simplifying the medication regimen [Van Dulmen et al. 2007]. ...

... On the one hand, hypoxia was reported to inhibit the Wnt/β-Catenin pathway via the direct repression of Wnts expression [32] and β-Catenin acetylation [33], competing binding of β-Catenin by Hif-1α and TCF-4 [34], etc. On the other hand, some studies showed that hypoxia cooperates with the Wnt/β-Catenin pathway to regulate many biological processes, such as the epithelial-mesenchymal transition [35][36][37] and cell proliferation [38,39]. ...

... The levels of two E-cadherin-associated proteins, b-catenin and p120cas, also decreased, while the protein levels of cytokeratin 18 (an epithelial marker) and vimentin (a mesenchymal marker) remained constant in both populations and higher extracellular levels of promatrix metalloproteinase-2 (pro-MMP-2) were found in the DU 145-F population. This decreased expression of E-cadherin and increased vimentin expression was confirmed in DU145 cells forming subcutaneous tumors in SCID mice (Luo et al., 2006;Zhao et al., 2011) and suggest the DU145-F cells have undergone an epithelialmesenchymal transition (EMT) to promote invasion and metastasis. Cancer cells may pass through EMTs to differing extents, with some cells retaining many epithelial traits while acquiring some mesenchymal ones and other cells shedding all vestiges of their epithelial origin and becoming fully mesenchymal (Kalluri and Weinberg, 2009). ...

... Previous studies have proposed the existence of some factors which may affect PSA serum levels such as the initiation of stating treatment and NSAID consumption (15,16). On the other hand, it was also suggested that some prostate manipulation, namely DRE and prostate biopsy, may also affect serum PSA levels (17,18). Nonetheless, a previous study led by Croccito et al. revealed low values of sensitivity and specificity for biomarker detection, regardless of prostate massage application (19). ...