Yaowen Sun's scientific contributions

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Publications (1)

The observation of morphology of HuMSCs self-assembled complex. The microscopic view of HuMSCs when cells reached confluency (Figure 1(a)). Morphological changes of HuMSCs after adding ascorbic acid for 3 days (Figure 1(c)) and 7 days (Figure 1(e)) were captured under the microscope. HuMSCs without treated with ascorbic acid were observed at day 3 (Figure 1(b)) and day 7 (Figure 1(d)). By day 28, a semitransparent and elastic cell-ECM complex was formed (Figure 1(f)). Scale bar, 200 μm.
Histological assessments and ultrastructure observation of the cell-ECM complex (28 days). HuMSCs distribution (black arrows) were presented in the cell-ECM complex by HE stain (Figure 2(a)). Plenty of collagen (green arrows) were stained pink in the cell-ECM complex by VG stain (Figure 2(b)). A lot of hyaluronic acid (yellow arrows) were also detected by AB stain (Figure 2(c)). SEM image showed the HuMSCs (white arrows) attached around the collagen fiber bundles (Figure 2(d)). Microscope scale bar, 100 μm, SEM scale bar, 10 μm.
mRNA expression of cell-ECM complex secreted growth factors. Six growth factors were measured with RT-PCR after adding ascorbic acid, including HGF (a), VEGF-α (b), KGF (c), TGF-β1 (d), collagen type I (e), and type II (f). The control group wasn’t treated with ascorbic acid while the experiment groups were treated with ascorbic acid. The cells of control group and experiment groups which were harvested at day 3, day 7, day 14, and day 28 were used for RNA analysis, respectively. Data were shown as mean ± SD (n = 3). *p < 0.05. **p﹤0.01. ***p < 0.001.
The protein levels of cell-ECM complex secreted growth factors. The control group wasn’t treated with ascorbic acid and the experiment groups were treated with ascorbic acid. With collecting the supernatant of control group, the supernatants of cell-ECM complex were also collected at day 3, day 7, day 14, and day 28 after adding ascorbic acid. Data were shown as mean ± SD (n = 3). *p < 0.05. **p < 0.01. ***p < 0.001.
Gross view and histology of cell-ECM complex implants. The cell-ECM complex prepared for implantation was cut into pieces, about 0.3 ml in a tube (Figure 5(a)). The gross views of cell-ECM complex implants for 2 months (Figure 5(b)) and 6 months (Figure 5(c)) were presented respectively. The samples were stained with HE, VG and AB stains. The cell-ECM complex implanted for 2 months were shown in (Figure 5(d)–(f) while cell-ECM complex implanted for 6 months were shown in (Figure 5(g)–(i). The cell-ECM complex was invaded by the host cells (red arrows). The blood vessels (black arrows) infiltrated in the implanted cell-ECM complex. Scale bar, 100 μm.

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An investigation of a self-assembled cell-extracellular complex and its potentials in improving wound healing
  • Article
  • Full-text available

January 2023

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1 Read

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1 Citation

Journal of Applied Biomaterials & Functional Materials

Journal of Applied Biomaterials & Functional Materials

Danyan Ye

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Yaowen Sun

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Lujun Yang

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Jing Su

Background To maintain and enhance the wound healing effects of mesenchymal stem cells (MSCs), a scaffold for hosting MSCs is needed, which ought to be completely biocompatible, durable, producible, and of human source. Objective To build a cell-extracellular matrix (ECM) complex assembled by human umbilical cord mesenchymal stem cells (HuMSCs) and to investigate its clinical potentials in promoting wound healing. Method HuMSCs were isolated and expanded. When the cells of third passage reached confluency, ascorbic acid was added to stimulate the cells to deposit ECM where the cells grew in. Four weeks later, a cells-loaded ECM sheet was formed. The cell-ECM complex was observed under the scanning electron microscopy (SEM) and subjected to histological studies. The supernatants were collected and the cell-ECM complex was harvested at different time points and processed for enzyme-linked immune sorbent assay (ELISA) and mRNA analysis. The in vivo experiments were performed by means of implanting the cell-ECM complex on the mice back for up to 6 months and the specimens were collected for histological studies. Results After 4 weeks of cultivation with ascorbic stimulation, a sheet was formed which is mainly composed with HuMSCs, collagen and hyaluronic acid. The cell-ECM complex can sustain to certain tensile force. The mRNA and protein levels of vascular endothelial growth factor-α (VEGF-α), hepatocyte growth factor (HGF), keratinocyte growth factor (KGF), and transforming growth factor-β1 (TGF-β1) were remarkably increased compared to monolayer-cultured cells. The implanted cell-ECM complex on mice was still noticeable with host cells infiltration and vascularization on 6 months. Conclusion Our studies suggested that HuMSCs can be multi-cultivated through adding ascorbic stimulation and ECM containing collagen and hyaluronic acid were enriched around the cells which self-assembly formed a cell-ECM complex. Cell-ECM complex can improve growth factors secretion remarkably which means it may promote wound healing by paracrine.

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