Ya-Qiao Wang's research while affiliated with Shanghai University of Traditional Chinese Medicine and other places

... According to the MS/MS spectra, these metabolites were primarily formed by hydrolysis. Since the catalytic efficiency and affinity of substrate(s) are fundamental for evaluating species-specific differences towards given biotransformation Wang et al. 2020), the enzyme kinetics for toddalolactone metabolism in liver microsomes from various species were established. Enzymatic kinetic analyses showed that oxidative metabolism profiles of toddalolactone for humans, rats, rabbits, mice and pigs followed the classic Michaelis-Menten kinetics, as evidenced by an Eadie-Hofstee plot, suggesting that toddalolactone was metabolized by the species through only one isozyme or two isozymes with the same K m value (Jiang et al. 2020), while such biotransformation in monkeys and dogs obeyed biphasic kinetics and substrate inhibition, respectively. ...

... Yet studies of Atgl −/− -and Hsl −/− mice revealed pronounced residual TG hydrolase activity in non-adipose tissues and members of the carboxylesterase (hCES/mCes) family are suggested to act as additional neutral lipid hydrolases [6][7][8][9][10][11][12]. Enzymes, that play key roles in lipid metabolism and/or metabolic activation of many drugs, like members of the human CES family, are very interesting pharmaceutical targets [10][11][12][13]. The human CES superfamily is divided into five families (CES1, CES2, CES3, CES4A, and CES5A) and all proteins have approx. ...

... Magnolol is widely used as medicine, because it has been identified as having anticancer, anti-inflammatory, and antimicrobial effects; liver protection; and anti-mutagenesis properties [49][50][51][52]. As an extract of traditional Chinese medicine, magnolol is often used as an oral drug and has shown obvious positive effects on human health [53,54]. Therefore, the safety of magnolol is certain. ...

... Our results show that the binding energy of three toxicants under study was similar to that documented for carbaryl, a carbamate pesticide potent inhibitor of cholinesterases in the presence of interfering enzymes such as chymotrypsin, lipase, proteinase K, and others (Dai et al., 2021). In contrast, these values were lower than reported for nevadensin, a natural flavonoid highly selective inhibitor for human CES1 and CES2 (Wang et al., 2018). In agreement with current results, it has been documented that NP is a potent inhibitor of CES present in the hepatic microsomal fraction or the hepatic post-mitochondrial fraction of different fish species and decapods (Solé and Sanchez-Hernandez, 2015). ...