Xin Guo's research while affiliated with Beijing Forestry University and other places
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Publications (4)
Allelic variation in floral quantitative traits, including the elements of flowers and fruits, is caused by extremely complex regulatory processes. In the genetic improvement of flare tree peony (Paeonia rockii), a unique ornamental and edible oil woody species in the genus Paeonia, a better understanding of the genetic composition of these complex...
Tree peonies native to China are a precious crop with ornamental, medicinal and edible oil properties, of which flare tree peony (Paeonia rockii) is one of the most significant germplasms in Paeonia. The development and application of expressed sequence tag-simple sequence repeat (EST-SSR) markers are very valuable for genetic and breeding applicat...
Research Highlights: This study, based on the first collection of cultivated Paeonia rockii (flare tree peony, FTP) germplasm across the main distribution area by our breeding desires, comprehensively evaluates these accessions by using phenotypic traits, expressed sequence tag (EST)-simple sequence repeat (SSR) markers and chloroplast DNA sequence...
Background:
Quantitative variation of floral organs in plants is caused by an extremely complex process of transcriptional regulation. Despite progress in model plants, the molecular mechanisms of quantitative variation remain unknown in woody flower plants. The Paeonia rockii originated in China is a precious woody plant with ornamental, medicina...
Citations
... Among them, the genetic linkage map of tree peony included 35 pairs from Guo et al. [33] and 68 pairs from Cai et al. [32], and a total of 95 markers remained after removing 8 duplicate primers. In addition, there were 20 pairs of association analysis primers based on Paeonia rockii populations, which contained 2-6 repeats of motifs, and these primers were developed based on transcriptome data [38]. Finally, a group of 115-pair primers was synthesized by Ruibio BioTech Co., Ltd. ...
... Various random molecular marker techniques, including amplified fragment length polymorphism (AFLP) [9], intersimple sequence repeat (ISSR) [10], random amplified polymorphic DNA (RAPD) [11], sequence-related amplified polymorphism (SRAP) [12], conserved DNA-derived polymorphism (CDDP) [12], and SSR markers [13][14][15] have been utilized for genetic diversity assessment, genetic relationship analysis, and population structure analysis in tree peony cultivars. As an ideal marker, SSRs have been frequently used in studies of many economically important trees due to their ubiquity, reproducibility, high level of polymorphism, codominant inheritance, and high level of transferability [16][17][18][19], such as for the assessment of genetic diversity [17,20,21], variety identification [22,23], genetic relationship classification [24,25], pollination [26], genetic-linkage maps [27,28], fingerprints [29], and evaluation of core germplasm resources [30,31]. ...
... The total length of unigenes was 102,497,172 nucleotides (nt), with an average length of 1,189 nt. The result is consistent with previous studies by Li (80,390) and Liu (66,563) on peony Liu et al. 2019). The N50 of unigenes was 1,780 nt (Fig. S1), which is similar to the length of P. lactiflorab 'Hangbaishao' (1473 bp) . ...