Wan-Chun Chen's research while affiliated with National Cheng Kung University and other places

... Nevertheless, there are numerous scientific articles relating ECM remodeling to mechanotransduction phenomena (Aitken et al. 2006;Remya and Nair 2020). Thus, external biomechanical stimuli translate into biochemical signals that initiate cellular processes such as growth, proliferation, cell differentiation (Yeh et al. 2017;Yanagisawa and Yokoyama 2021;Sthanam et al. 2022), and embryonic morphogenesis (Brunet et al. 2013;Mitrossilis et al. 2017;Pukhlyakova et al. 2018;Bailles et al. 2019). ...

... Myofibroblasts could be originated from various cell sources, including tissueresident fibroblasts (Kuppe et al, 2021), perivascular mesenchymal cells (Humphreys et al, 2010;Kuppe et al., 2021), and macrophages (Meng et al, 2016) during fibrosis. Our previous studies showed that mechanical properties and components of substrates play crucial roles in myofibroblast activation (Chen et al, 2014(Chen et al, , 2015. However, most in vitro studies on myofibroblast activation were conducted on stiff culture dish. ...

... As can be seen from the results of current study, cells under influence of dECM and RdECM were found to proliferate and migrate into branched tubular structures with mean tubular branching index higher in RdECM compared to dECM, which indicates significantly higher In-Vitro angiogenetic activity of RdECM. These findings agree with [56,57] which reported that ECM acts as depot of growth factors (GFs) including angiogenetic factors which are released in sustained release fashion with degradation with ECM. Persistent and sustained release of GFs promotes epitheliogenesis, angiogenesis and cellular migration/differentiation which promotes wound healing, owing to these desired characteristics ECMs serve as ideal materials for fabrication of materials for organ/tissue regeneration. ...

... Kidneys are of importance for whole body homeostasisbecause of the important functions in processing digestion products, water balancealong with BP regulation. Hence newer strategies to treat the biggest problems involving kidneys is needed,of which chronic kidney diseaseresulting in end stage renal replacement therapyand eventual transplantation=>massive load on various health care systems.Leonard et al used very novel tools to improve end result of classical approaches, e.g membraneless dialysisstrategy was developed opening possibility to create wearableblood processing devices [115,150].Culture of kidney cells in tubular structureswhich ape the organ structure and function are developed by microfluidics.Also microfluidics can be used to model diseases ,metabolism studies,insight overkidney cell toxicityand renal clearance [151][152][153][154][155][156][157].Renal excretion and metabolism are the actual subjects of preclinical safety studieswith goal of investigating drug pharmacokineticsin vivo like pathophysiological conditions. Hencedevices utilizing microfluidics can be of use in co-culturing different cell types Along with impact on the recreation of multiorgan system to studysystem interactions where kidneys and also liver can be incorporated [158]. ...