W Göhde's research while affiliated with Universitätsklinikum Münster and other places

... In the BC-C, there are many publications with only occasional [13,16,17]. The only study with a large number of cases [18] showed average of 19% of aneuploidy in 509 BCC studied. Unfortunately, this is a service of summons and could not recover it or explain the reason for the signifycant difference with our findings (73.8% of samples of BCC with cellular aneuploidy). ...

... Suitable clones yield an excellent resolution which allows the identification of all chromosome types and opens a way for the detection of persisting aberrations. In earlier approaches to utilize flow cytometric chromosome histograms for mutagenicity studies, the authors analyzed the first mitoses after treatment and used the broadening of the peaks and/or the increasing background for the quantitation of the mutagenic effect [2][3][4][5][6][7]. These approaches remained unsatisfactory, because the sensitivity was low and the specificity was impaired by cytotoxic effects. ...

... Three Euler angles (Ω, Ψ, ω) are defined to establish the relationship between them. As can be seen, the orientation of the z-axis with regard to the lab system is determined by angles Ω and Ψ. Normally, an ideal two-dimensional (2D) model, where a degenerate transition dipole orientates isotropically in two dimensions and thus gives rise to a perpendicular " dark axis " that does not couple to the light field [16], is sufficient to characterize the emission behavior of a spherical QD (I z =0, I x =I y ≠ 0, I is the emitting intensity). However, when the 2D transition moment is not of ideal circular shape, the ideal 2D model needs to be modified by introducing an elliptical parameter [12]. ...

... Fluorescence intensity images of single hypericin molecules on glass and embedded in PVA are shown in Fig.2 Intensity fluctuations in the image patterns occur since the molecule is in a dark state for a certain amount of time, e.g. the triplet state or in another non-emissive states. [35][36][37][38] This blinking is characteristic for a SM and, in addition to the single step photo bleaching, gives an indication that a SM is observed. In order to investigate the blinking dynamic of hypericin, we have recorded intensity time traces of SMs. ...

... Flow-cytometric samples were prepared from fully developed fresh leaves (Galbraith et al. 1983) and processed according to the two-step protocol of Otto et al. (1981), with the concentrations of buffers, dyes and other modifications described in detail by Šmarda et al. (2008) Temsch et al. (2022: Table 1). For the genome size and ploidy level estimations, propidium iodide was used as a fluorochrome, and for the genomic GC content estimation, the samples were coprocessed with DAPI (4',6-diamidino-2-phenylindole) fluorochrome (Šmarda et al. 2008(Šmarda et al. , Sliwinska et al. 2022). ...

... The Counter uses a 'no lyse, no wash' procedure for CD4 counting. 16 EDTA-anticoagulated blood (fifty microliters) were added to 10 μl of monoclonal antibodies. After 15 min of incubation, 1ml of no lyse dilution buffer was added and the sample tube was attached to the Counter for automated counting. ...

... Les applications de ces méthodes multiplexées qui permettent l'identification et la quantification simultanée de plusieurs ions ou macromolécules concernent aussi l'identification d'activités enzymatiques et les analyses de polymorphisme. Avec les microbilles utilisées comme support réactionnel, les domaines d'application de la cytométrie en flux vont ainsi de l'analyse cellulaire à l'analyse moléculaire (Lizard et al., 2003). ...

... 32,33 Light excitation and collection can both be accomplished through a nanoaperture at the apex of the otherwise metalcoated tapered optical fiber that serves as the probe. 34 While this method has been previously implemented into cryogenic UHV SPM systems, 35,36 here we will instead focus on apertureless methods. ...

... Up to the present time SMSM has been realised in spectroscopy and microscopy techniques (see e.g. [113][114][115][116]), most commonly used of which will be shortly discussed below. ...

... The main method that has been used so far in anharmonic vibrational spectroscopy calculations for biological molecules [9], small and large, is the vibrational self-consistent field method, including its extensions such as the correlation-corrected VSCF [10]. Applications to molecules where an analytic force field is applied are very effective for monosaccharides, peptides and even proteins [11,12]. Based on these studies this field can be thought of being one of the potential functions which may play a significant part in describing the functioning of a mitotic spindle. ...