V. Naithani's research while affiliated with Venus Medicine Research Centre and other places

The study was performed to assess the acute dermal toxicity of Trois ™ in Newzealand white rabbit. Test substance was applied as such to the shaven skin of group of rabbits at the dose of 2000 mg/Kg body weight. Control group of animals were similarly treated but only with base. Following dosing up to 14 days the rabbits were observed for mortality and clinical sign of toxicity. No visible signs of toxicity after treatment were observed on the animals of both control and treated animals up to 14 days. Various haematological and biochemical parameters were evaluated and found to be in the normal limit, which indicates that no sign of toxicity in NewZealand white rabbits after 14 days treatment in respect to control group, proving safety of Trois TM in topical application.

To evaluate the effect of Acnano drug on status of antioxidant enzymes and other biochemical parameters and its comparison with clearasil drug in the plasma of acne induced rat model. Twenty four rats were selected and divided into three groups. Group I was control treated group, whereas group II and group III were infected plus clearasil as well as infected plus acnano treated group. Acne was induced by the topical administration of cyproterone acetate drug for three weeks and determine the antioxidant enzymes along with free radical mediated damage (malonaldialdehyde and myloperoxidase activity) as well as hepatic and renal enzymes was done in the plasma of all groups. Our results showed a significant decrease in the enzyme activities along with increased free radical mediated damage and hepatic and renal enzymes in both acne induced infected group as compared with control group. After treatment with clearasil and acnano drug for 7 days, the enzymes activities along with free radical mediated damage as well as hepatic and renal enzymes, were improved in both infected plus treated groups. When acnano treated group was compared with clearasil treated group, these enzyme activities along with free radical mediated damage and other biochemical parameters were increased significantly in acnano treated group after 7 days treatment. Our findings concluded that acnano drug was found to be effective and equivalent to clearasil drug because of their ability to reduced oxidative stress along with increased the enzymes activity and improved liver and kidney function tests due to hormonal imbalance during administration of anti-androgenic drug.

Plants have been used in ethno-medicine since many years and there has been an increasing interest in medicinal plants and their traditional use. The present study compared efficacy of Ampucare with that of silver sulfadiazine in partial thickness burn in rats. Adult male Sprague-Dawley rats were divided into three groups: control group (Normal saline treated group, Ampucare treated group and Silver sulfhdiazine treated group. Complete healing of burn wound was observe in all the animals. A significant decrease in wound size was observed in Ampucare treated and Silver sulfadiazine treated group as compared to contraol group on day 14. Both in control group and Silver sulfadiazine treated group not a single animal showed complete healing of the wound on day 14. Results suggested that Ampucare is significantly effective in treatment of partial thickness burn wounds than silver sulfadiazine.

This study was undertaken to determine the antimicrobial susceptibility of Acnano (a polyherbal nano- emulsion) against acne causing microbes Propionibacterium acnes, Staphylococcus aureus and Staphylococcus epidermidis along with some other dermal disease causing microbes such as Pseudomonas aeruginosa, Morganella morganii, Enterobacter cloacae, Escherichia coli, Citrobacter braakii, Klebsiella pneumoniae, Acinetobacter baumannii, Proteus vulgaris, yeast such as Saccharomyces cerevisiae and fungi Candida albicans and Aspergillus niger. The agar well diffusion method, approved by NCCLS with the modification, was used. Student t test was performed using One way Analysis of Variance (ANOVA). This study showed that Acnano possesses potent antimicrobial activity against bacteria as well as fungi.

Oxidative stress and free radicals have been implicated in impaired wound healing. Ampucare is a polyherbal formulation that possesses anti-inflammatory, antioxidant and wound healing properties. To determine the antioxidant property of ampucare and wound healing parameters in mice. The present study was undertaken to assess the efficacy of ampucare and betadiene in wound healing process as well as determine the antioxidant enzymes activities along with MDA level and biochemical parameters in mice. Mice were divided into three groups of twelve mice each. Control group (without treatment) and experimental group (treated with ampucare and betadine) were subjected to biochemical and enzymatic parameter in granulation tissue of mice on 7th and 14th days treatment Our results showed that both drug treated groups significantly increased the levels of hydroxyproline, hexosamines, total protein, antioxidant enzymes (SOD, Catalase) along with a significant decreases in MDA level in granulation tissue when compared with control group after 7 and 14 days treatment. These levels were significantly increased along with a decrease in the MDA level in granulation tissue of ampucare treated group in comparison to betadiene treated group after 7 and 14 days treatment The results suggest that ampucare has antioxidant and wound healing properties, which may be responsible for faster wound healing in granulation tissue than betadiene.

A clinical trial survey of patients treated with ampucare, was conducted by our research centre. The main objective was to check the efficacy and wound healing trend of ampucare in patients suffering from diabetic leg ulcer, venous ulcer, arterial ulcer, burns, cellulitis, traumatic wounds, post-operative wounds and bedsores. For this purpose Forty patients, male (n=29) and female (n=11) were selected with age ranging from 18-70 years in the case of male volunteers and 20-68 years old, in the case of female volunteers. Inclusion criteria were (1) Subjection of legal age (2) Written consent of the volunteer (3) Washout period of one week and (4) Whether immunized for tetanus in the last 5 years. Exclusion criteria were (1) Osteomyelitis underlying ulcer (2) Discharge of bile, pancreatic, or intestinal secretion and (3) Malignancy in ulcer. Current drug therapy, date of randomization, persistance and location of wounds etc. were some of the other factors which were taken into account while conducting these clinical trials. Each patient was given a code number PN1 to PN40. They were categorized into 8 categories i.e., diabetic leg ulcer (n=25), venous ulcer (n=06), arterial ulcer (n=02), burn cases (n=01), traumatic wounds (n=02), cellulitis (n=01), post operative wounds (n=01) and bedsores (n=02). Persistance of wound ranged from 14 to 108 weeks in the case of diabetic leg ulcer; 10 to 60 weeks in the case of venous ulcer and from 36 to 75 weeks in the case of arterial ulcer. Slough was absent or yellow, white or green in color. Most of the wounds had cavity and infections. Initial size (area in cm2) of the wound was measured and served as control and area of the wound after every week was recorded. Results obtained were wonderful and proved the miraculous healing efficacy of ampucare in almost all types of non-healing wounds.