Susanne M. Mumby's research while affiliated with University of Texas Southwestern Medical Center and other places

... Based on the distribution and properties of sEH, Gill and Hammock correctly hypothesized that it had an endogenous role in regulating chemical mediators. Early on, they thought that it might be a scavenger enzyme that hydrolyzed terpene epoxides such as squalene dioxide and lanosterol epoxide ( Dean et al., 1967;Hammock et al., 1980b). Involvement of sEH in these reactions has not ruled out, but the discovery by Mumby and Hammock (1979) that 1,2-disubstituted epoxides were turned over far faster than trisubstituted and tetrasubstituted epoxides led to the hypothesis that fatty acid oxides were endogenous substrates, which was quickly confirmed by Hammock (1979). ...

... Since ABE and acyl-RAC are easier, convenient, and timesaving, by far, hundreds of S-palmitoylated proteins have been identified, and their S-palmitoylated sites have been annotated (Linder et al., 1993;Mumby and Muntz, 1995;Forrester et al., 2011;Zhou et al., 2019). However, there are certain disadvantages of ABE, including the high background caused by captured non-S-palmitoylated proteins and the fact that the type of lipid attached to the protein cannot be accurately identified and needs further analysis. ...

... August 2022 | Volume 13 | Article 836593 5 (Nishimura et al., 1991), this suggests that it may play a similar role in the formation of AD. THBS1 is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions (Mumby et al., 1984;Staniszewska et al., 2007), In addition, THBS1 can activate TGFβ and matrix metalloproteinase (MMP), which mediates the interaction between cells and matrix and participates in angiogenesis, proliferation and platelet aggregation (Savarimuthu Francis et al., 2011).The finally gene CDH5, encodes a classical cadherin of the cadherin superfamily, it is essential for vascular integrity and endothelial function (Mao et al., 2013). The GO and KEGG enrichment analysis also displayed that the above genes play an crucial role in regulating extracellular matrix and mediating inflammatory signaling pathways. ...

... The procedure described by Linder et al. was followed (Linder et al., 1995). Briefly, COS-7 cells were transiently transfected with pGFP-N1-EFR3A or pEGFP-N1-EFR3B. ...

... Radiolabeling and Immunoprecipitation-Palmitate incorporation was measured by incubating confluent cells with 0.5-1 mCi/ml [ 3 H]palmitate (PerkinElmer Life Sciences) in medium containing nonessential amino acids, 20 mM Hepes (pH 7.2), 25 g/ml cycloheximide, and 10% calf serum (36). Labeling was stopped at times described by rinsing cells twice with Tris-buffered saline and lysing cells in a high SDS-RIPA buffer (0.15 M NaCl, 50 mM Tris, pH 7.0, 1% aprotinin (Calbiochem), 1% Na-deoxycholate, 1% Nonidet P-40 (U. S. Biochemical Corp.), 0.5% SDS). ...

... Although not widely reported on, it is generally understood by B cell biologists that B cells undergo some amount of gene expression change during in vitro culture. Indeed, this aspect of cultured B cells presented a major challenge to efforts by the Alliance for Cell Signaling (19,20) to dissect the signaling circuits in mouse B cells. The pathways involved in causing these gene expression changes are not understood. ...

... However, anecdotal results on esterase inhibition as well as substrate selectivity is not a reliable basis to define a juvenile hormone esterase. Very early studies on the JHEs of cockroaches and houseflies cautioned against this trend since they were sensitive to very different inhibitors (Hammock et al., 1977a,b; Yu and Terriere, 1978; Mumby et al., 1979; Sparks and Hammock, 1980 ). This study and the previous studies of Sparks and Hammock (1980); Stauffer et al. (1997), and Thomas et al. (2000) on T. molitor also caution that there is likely to be a great variation in the sensitivity of JHEs (both among and within different species) to inhibitors. ...

... G␣ t1 * was expressed and purified as previously described (41). To obtain myristoylated G␣ t1 * (myrG␣ t1 *), BL21-codon plus Escherichia coli cells were co-transformed with kanamycin-resistant plasmid pbb131 expressing yeast N-myristoyl transferase (42). ...

... Early criticisms of this idea were soon substantiated by reports of insect populations that were already resistant to other insecticides were subsequently found to be resistant to JH or its analogues (Ellis, 1968;Dyte, 1972;Schneiderman, 1972). Resistance to methoprene in various Dipterans (Hammock et al., 1977;Ashok et al., 1998;Cornel et al., 2002;Kristensen and Jespersen, 2003;Cetin et al., 2009) and Coleopterans (Benezet and Helms, 1994;Daglish, 2008) has been detected. Recently, resistance to methoprene by R. dominica was reported from Australia; a resistant population exhibited a dose response for mortality and progeny production with complete suppression of the progeny at 40 ppm (Daglish et al., 2013). ...

... The presence of both methoprene and JH III in the JH hydrolysis assay did not affect the ability of CqJHE to metabolize JH III indicating that methoprene does not inhibit or otherwise interact with JHE as was previously hypothesized [28]. These findings are consistent with previous studies showing that methoprene is a poor inhibitor of authentic semi-purified JHEs and purified JHE (with JH III substrate) from multiple insect orders including Blattaria, Lepidoptera, Coleoptera, and Diptera [7,33,38]. In similar competition experiments (i.e., when both JH and the JHA are present at the same molar ratio), hydroprene and fenoxycarb (composed of ethyl esters) and kinoprene (composed of a propynyl ester) were also unable to inhibit or only poorly inhibited the hydrolysis of JH III (Fig. 5A). ...