Siyun Cheng's research while affiliated with Xuzhou Institute of Technology and other places
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Publications (11)
Introduction
Ultrasensitive bacterial detection methods are crucial to ensuring accurate diagnosis and effective clinical monitoring, given the significant threat bacterial infections pose to human health. The aim of this study is to develop a biosensor with capabilities for broad-spectrum bacterial detection, rapid processing, and cost-effectivene...
Rapid, direct and sensitive detection of foodborne bacteria in complex samples is still challenging. Here, we reported a universal surface-enhanced Raman scattering (SERS)-based lateral flow assay (LFA) for highly sensitive detection of foodborne bacteria in food and environmental samples using wheat germ agglutinin (WGA)-modified Fe3O4@Au (Au@MNP-...
A novel surface-enhanced Raman scattering (SERS)-based analytical technique was proposed to simultaneously detect two highly pathogenic bacteria, namely, Staphylococcus aureus (S. aureus) and Listeria monocytogenes (L. mono) by using a dual-recognition pattern with wheat germ agglutinin (WGA) and nucleic acid aptamers. WGA was modified onto Fe3O4@A...
Here, we proposed a silica–quantum dot (QD)-based fluorescent lateral flow immunoassay (LFA) method with high sensitivity for the simultaneous qualification of Salmonella typhimurium and Escherichia coli O157:H7 in food samples. The silica–QD nanobead ([email protected]) with dual-QD shell was introduced into the two-channel LFA strip as the advanc...
Rapid, reliable and sensitive detection methods for pathogenic bacteria are strongly demanded. Herein, we proposed a magnetically assisted surface enhanced Raman scattering (SERS)-label immunoassay for the sensitive detection of bacteria by using a universal approach based on free antibody labelling and staphylococcus proteins A (PA)-SERS tags orie...
Purpose
Candidemia infection is common in the clinic and has a high mortality rate. Candida albicans, Candida tropicalis, and Candida krusei are very important and common pathogenic species. Candida is difficult to isolate from clinical samples and culture, and immunological detection cannot distinguish these related strains. Furthermore, Candida h...
Purpose
The continuous emergence of carbapenem-resistant Klebsiella pneumoniae (CRKP) has become a serious public health problem globally, especially for children, but data on CRKP infection in pediatric patients are limited. This study aimed to identify epidemiological and molecular patterns of CRKP among pediatric patients in Jiangsu province, Ch...
Background: The continuous emergence of carbapenem-resistant Klebsiella pneumoniae (CRKP) has become a serious public health problem globally, especially for children, but data on CRKP infection in pediatric patients are limited. This study aimed to identify epidemiological and molecular patterns of CRKP among pediatric patients in Jiangsu province...
Objective:
To investigate the prevalence of Salmonella Typhi and Paratyphi resistance to quinolones and characterize the underlying mechanism in Jiangsu Province of China.
Methods:
Antimicrobial susceptibility testing was performed using Kirby-Bauer disc diffusion system. Quinolone resistance-determining region (QRDR), plasmid-mediated quinolone...
Citations
... With continuous improvements in test strip detection technology, detection methods for other common pathogenic bacteria have also been established, such as V. parahaemolyticus [38] and Campylobacter jejuni [39]. Today, test strip detection technology is widely used in detecting food pathogens. ...
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... × 10 7 CFU/mL. In addition, colorimetric [48], SERS [49], and test strip [50] detection techniques were also combined with aptamer to achieve the detection of Listeria monocytogenes. ...
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... Their wide use in POC development is a direct result of their affordability, usability, speedy results and straightforward interpretation. Heart illness, monitoring food toxins, food poisoning, bacterial infection, viral infection and many other conditions are examples of applications for LFDs [40][41][42][43][44]. ...
... [40][41][42] The characteristic Raman peaks of DTNB were located at 1333 cm −1 also aligns with previous research. [43][44][45] It was important to highlight that the strongest SERS signals were observed in AuNRs@4-MBA/DTNB@SiO 2 @Ag. This indicated that Ag nanoparticles can further enhance the characteristic Raman peaks of the SERS probes significantly. ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/1093927998369792-1637824167219_Q64/Wang-Shu-24.jpg)
... The surface of the Candida has a negative charge. Taking advantage of this characteristic, Hu et al. invented a rapid detection method for Candida (Hu et al. 2021). They synthesized Fe3O4@PEI microspheres, adsorbed the Candida on their surface to form Fe3O4@PEI@Candida, and then adsorbed AgNPs substrate on the complex surface to form Fe3O4@PEI@Candida@AgNPs. AgNPs, as an enhancing substrate, identified the Candida genus through SERS fingerprint spectra. ...
... In this study, CTX-M-14-positive K. pneumoniae EE25K_SQ190 belonged to ST35. Although this clone has been previously identified in China, Romania, Yemen, Israel, France, Spain and Thailand (Marcade et al., 2013;Cubero et al., 2016;Alsharapy et al., 2020;Frenk et al., 2020;Kong et al., 2020;Shen et al., 2020;Surleac et al., 2020;Zhong et al., 2020;Leangapichart et al., 2021), phylogenomic analysis clustered (353-354 SNP differences) EE25K_SQ190 with a human clone identified in Turkey in 2014 (Figure 2, Supplementary Table 3). ...
... Previous report showed that parC T57S is a spontaneous compensatory mutation, resulting in resistance to nalidixic acid but sensitivity to ciprofloxacin in Salmonella isolates (60,61). However, accumulation of mutations in gyrA or parC genes, together with parC T57S, resulted in complete resistance to ciprofloxacin in different Salmonella serovars (62,63). The resistance to quinolones has been widely reported in Salmonella serovars, especially in serovars frequently isolated from poultry sources such as S. infantis and S. Kentucky, probably due to the selective pressure exerted on the microbial communities of poultry farming where the use of quinolones as therapeutic are still present (64). ...
