Shaohu Tang's research while affiliated with Southwest University in Chongqing and other places

... Abscisic acid was measured as described before 42 ; 500 mg of plant cells were mixed with liquid N and 1.5 mL of 70% methanol and stirred overnight at 4 °C. Methanol was evaporated and 50 µL of borate buffer (pH 8.5) was added and a sample was partitioned with ethyl acetate three times. ...

... Studies in Arabidopsis have identified a group of NAC (NAM, ATAF1/2, and CUC2) domain proteins that are essential for xylem cell formation, including vascularrelated NAC domain 6 (VND6), VND7, secondary wallassociated NAC domain protein 1 (SND1, also called NAC secondary wall thickening promoting factor 3 (NST3)), SND2, SND3, and xylem NAC domain 1 (XND1) (Kubo et al., 2005;Zhong et al., 2006Zhong et al., , 2008Mitsuda et al., 2007;Zhao et al., 2008;Zhao et al., 2017). These NAC domain proteins have also been implicated in xylem formation in species of the woody plant genus Populus, commonly referred to as poplar (Grant et al., 2010;Ohtani et al., 2011;Zhong et al., 2011;Wang et al., 2013;Zhao et al., 2014;Yang et al., 2015;Takata et al., 2019). For example, transgenic P. tomentosa plants overexpressing PtoVNS11 (VND-, NST/SND-, and SMBrelated protein 11, also known as SND1-A2) have a wider xylem zone compared with controls (Ohtani et al., 2011;Yang et al., 2015), and poplars overexpressing XND1 (previously named PopNAC128 in poplar) show severe stunting associated with a reduction in xylem tissue (Grant et al., 2010), suggesting that SND1 and XND1 have different regulatory roles in xylem development. ...

... Similarly, cerium induces cellular apoptosis and Taxol biosynthesis in Taxus cuspidata suspension cultures (Yang et al. 2009). In a study in which the effect of Pr on Scutellaria viscidula on the production of flavonoid, peroxidase, polyphenol oxidase, and phenylalanine ammonium lyase was determined, it was concluded that Pr could be used as an elicitor and with high effectivity in the production of pharmaceutical raw materials (Lei et al. 2011). These data indicate that REEs can increase secondary metabolite production, especially in medicinal essential plants. ...

... Different plants had different sensitivity to Agrobacterium concentration, and appropriate Agrobacterium concentration and infection time were very important to improve the transformation rate. Agrobacterium concentration OD600 for orchid [16], potato [17], kiwifruit [18] and switchgrass [19] transformation methods was 0.8, 0.5, 0.5 and 0.8 respectively, and infection time was 30 min, 8 min, 10 min and 20 min respectively. Whereas Agrobacterium concentration OD600 and infection time in this experiment was 0.6 and 10 min respectively. ...

... The present results corroborated well with other studies on Actinidia spp. where BA as a cytokinin all alone or along with NAA, IBA, and GA 3 resulted in proliferation rates of 8.63 in A. chinensis cultivar Red Sun, 3 in A. arguta cultivar Issai, 2.5 in A. melanandra Franch., 3.5 in A. rubricaulis Dunn., 6.2 in A. deliciosa, and 5.06 in A. deliciosa cultivar Guichang (Zhao et al. 2013;Hameg et al. 2017;Bourrain 2018;Deb and Gangmei 2019;Zhong et al. 2021). Contrarily to this, by augmenting MS medium with aromatic group cytokinin, for example meta-topolin (mT) in place of BA and Zeatin, an improved shoot multiplication rate of 5.57 and 6.89 was found in A. chinensis variety chinensis 'Zes006' (Saeiahagh et al. 2019). ...

... The expression of SoCHS1 in Syringa oblata was the highest before flowering, and the transcription level was highest in the corolla lobes and calyx; overexpression of SoCHS1 in tobacco led to a darker corolla color of flowers, suggesting that this gene may be involved in anthocyanin synthesis (Wang et al., 2017b). In poplar, PtrCHS4 systemically responds to traumatic stress, with a 2.4-fold increase in transcript levels observed after 24 h of trauma treatment (Sun et al., 2011). Heterologous overexpression of EaCHS1 in Eupatorium adenophorum in tobacco increases flavonoid accumulation downstream of CHS and promotes the up-regulated expression of related genes; maintaining ROS homeostasis during seed germination and root development regulates tobacco tolerance of salt stress (Lijuan et al., 2015). ...