Sang Suk Kim's research while affiliated with National Institute of Horticultural and Herbal Sciences and other places

Publications (75)

Article
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Citrus fruits have a distinctive flavor and can convey health benefits because of their unique phytochemicals. Phytochemical profiles are influenced by many factors, including variety and environmental growing conditions; however, the effect of the cultivation methods on the phytochemical profile of Satsuma mandarin (Citrus unshiu) has received lit...
Article
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This study discusses the challenge of distinguishing between two high-quality mandarin cultivars, ‘Asumi’ and ‘Asuki’, which have been introduced and cultivated in Korea after being developed through crossbreeding in Japan. Owing to genetic similarities resulting from crossbreeding between the same parent cultivars, it is challenging to differentia...
Article
Full-text available
Although numerous citrus varieties have recently been developed to enhance their quality, information on their quality characteristics is limited. We assessed the quality characteristics of Yellowball, a novel citrus variety, by evaluating its appearance, storability, sensory properties, functionality, and metabolite profiles and then comparing the...
Preprint
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Although numerous citrus varieties have recently been developed to enhance their quality, information on their quality characteristics is limited. We assessed the quality characteristics of Yellowball, a novel citrus variety, by evaluating its appearance, storability, sensory properties, functionality, and metabolite profiles and comparing these ch...
Article
Yellowball (Citrus hybrid cv. Yellowball ) is a new citrus hybrid between Haruka (C. tamurana × natsudaidai ) and Kiyomi (C. unshiu × sinensis) and is known to possess strong antioxidant activity. However, detailed information on the antioxidant components of its peel has not yet been reported. This study evaluated the antioxidant activity of the p...
Article
This study emphasized not only the basic concept that the sunburn of fruit was caused by fruit skin temperature rising above its tolerable threshold but also that the threshold was variable, namely, fruit became more vulnerable to sunburn on its own as the growth period progressed. These main results were derived from two major functions: first, mu...
Article
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Sunburn causes fruit browning and other physiological symptoms, reducing fruit production and quality. Therefore, we aimed to investigate the anatomical differences and abiotic stress responses in ‘Nichinan 1 gou’ satsuma mandarin (Citrus unshiu Marc.) according to the severity of sunburn damage (five grades: control, no sunburn; I to IV, increasin...
Article
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Citrus fruits undergo significant metabolic profile changes during their development process. However, limited information is available on the changes in the metabolites of Citrus unshiu during fruit development. Here, we analyzed the total phenolic content (TPC), total carotenoid content (TCC), antioxidant activity, and metabolite profiles in C. u...
Article
For analyzing the useful components in kanpei and natsumi, volatile as well as non-volatile components were analyzed using HPLC and GC/MS. Kanpei and natsumi juices contain free sugars and organic acids which are involved in imparting sweet and sour flavors. Analysis of the free sugar content revealed that the sucrose content was 6.53±0.31 g/100 g...
Article
Fruit sunburn, which is caused by high temperatures and strong sunlight, will increase under climate change due to warmer temperatures. In this study, to facilitate effective sunburn management, the occurrence of sunburn was predicted. The study included an investigation of sunburn-inducing conditions and the estimation of fruit skin temperatures i...
Article
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Many citrus varieties are hybridized to improve their quality and to overcome the effects of climate change. However, there is limited information on the effect of the chemical profiles of hybrid varieties on their quality. In this study, we analyzed 10 citrus varieties and evaluated the correlation with their general characteristics and antioxidan...
Article
Recently, colder weather has been occurring frequently in winter due to climate change, and this has a negative effect on the production of late maturing citrus cultivar such as Shiranuhi mandarin. Therefore, we investigated how freezing at a low temperature affects cold tolerance, fruit characteristics, amino acid content, volatile compounds, and...
Article
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Operating room (OR) nurses’ perioperative competence is vital in operation and patient care. This cross-sectional descriptive study aimed to identify perioperative competencies and educational needs for improving competencies according to the clinical ladder. A total of 318 OR nurses in Korean tertiary hospitals were recruited. Data from the self-r...
Article
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Sciatic nerve injury (SNI) leads to sensory and motor dysfunctions. Nobiletin is a major component of polymethoxylated flavonoid extracted from citrus fruits. The role of nobiletin on sciatic nerve regeneration is still unclear. Thus, the purpose of this study was to investigate whether nobiletin increases DRG neurite elongation and regeneration-re...
Article
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Excessively high temperatures frequently occur between spring and late fall because of global warming. These problems have a negative effect on fruit production capacity and actual production. However, there is a lack of information on the effects of excessively high temperatures (exceeding the optimum range) on the growth of citrus fruits. Thus, t...
Article
The objectives of this study were to trace the shift in bud burst and full bloom dates and provide a countermeasure for the suitable prediction of these representative phenological indices for citrus trees. The heat accumulation value was calculated by the growing degree day (GDD) model with six base temperatures (Tb) from 5 to 17.5℃ in 2.5℃ interv...
Article
Global warming is contributing to extreme weather events such as abnormally colder weather, but there is lack of information on the freezing tolerance of citrus grown in Korea. Therefore, it is necessary to prevent damage from the cold in advance, by acquiring information on the critical temperature at which 50% injury (LT50) occurs for citrus vari...
Article
A new early maturing satsuma mandarin (Citrus unshiu Marc.) ‘Haryejosaeng’ was developed at the Citrus Research Institute, Korea. The present study investigated the variation in total phenolics (TP), flavonoid, and antioxidant activity of fruits of ‘Haryejosaeng’ according to the harvest from September 2018 to December 2018, in Jeju. Colorirmetric...
Article
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A Gram-stain-negative, facultatively anaerobic, non-motile and rod-shaped bacterial strain, designated THG-SD5.5T, was isolated from a soil sample collected in a tangerine field, Republic of Korea. According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Chitinophaga and to be closely related to Chiti...
Article
A new mandarin hybrid cultivar called ‘Shinyegam’ was developed from a cross between ‘Kiyomi’ (Citrus unshiu × C. sinensis) and ‘Wilking’ (Citrus reticulata) at the Citrus Research Institute of the National Institute of Horticultural and Herbal Science in 2012. The ‘Shinyegam’ was extracted with 70% ethanol (SYGE), and polyphenol content was measur...
Article
Lipopolysaccharide (LPS)-induced activation of murine RAW 264.7 cells results in the production of pro-inflammatory cytokines including PGE2, IL-6, TNF-a, and IL-1β. In this study, we investigated the anti-inflammatory effects of the Olea europaea extract from Jeju island using LPS which induces an inflammatory response. To examine the potential an...
Article
Objective In this study, we evaluated the anti-inflammatory effect of Shiranuhi flower in RAW 264.7 cells. Methods The effects of the extracts and solvent fractions on cell viability and LPS-induced inflammatory responses were investigated in RAW 264.7 cells. Results The results showed that the ethyl acetate fraction (HEF) significantly decreased...
Article
Hylotelephium erythrostictum is commonly used as a medicinal herb. In this study, H. erythrostictum leaf (HEL), branch (HEB), root (HER), and above ground (HEAG) extracts were evaluated for their antioxidant properties. The antioxidant activities were assayed by three methods based on scavenging of DPPH, ABTS and superoxide anion radical. HEAG extr...
Article
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Auraptene is the most abundant naturally occurring geranyloxycoumarin. It is primarily isolated from plants belonging to the Rutaceae family, many of which, such as citrus fruits, are used as food in many countries. Auraptene is a biologically active secondary metabolite that possesses valuable properties. The aim of this study was to investigate t...
Article
Citrus unshiu is an economically important fruit on Jeju Island, Korea, but byproducts are a major source of agricultural waste. The aim of this study is to examine changes in phytochemical, antioxidant, and antibacterial activities of four C. unshiu byproducts: citrus flesh byproduct (CFB), fermented citrus flesh byproduct (FCFB), citrus peel bypr...
Article
This study was conducted to investigate total flavonoid content, flavonoid composition, and free radical scavenging effects of by-products from five orange cultivars during the period September 2015 to February 2016. Total flavonoid content was highest in peel extracts from immature fruit harvested in September. Total flavonoid contents of all cult...
Article
Citrus platymamma hort. ex Tanaka is widely used in traditional Korean medicine because of its medicinal benefits including an anti-inflammatory effect. This study aimed to evaluate changes in the flavonoid content and anti-inflammatory activities of C. platymamma during its harvest period. Fruit peel samples were obtained between September 2015 an...
Article
Activity of the noncontacted low temperature atmospheric pressure surface discharged plasma (LASDP) converts stable gas to ionized gas known as discharge or plasma. This ionized gas exhibits the antimicrobial activity. We examined the effects of 3 different storage treatments for 80 days on ‘Setoka’ : ambient storage (AS), low-tempperature storage...
Article
In this study, to evaluate the usefulness of Rhododendron weyrichii Maxim.as a whitening agent, the whitening effects of its extracts were investigated in alpha-melanocyte-stimulating hormone (α-MSH)-induced B16F10 melanoma cells. No toxicity was noted in either B16F10 melanoma cells or HaCaT keratinocyte cells that were exposed to the hot water or...
Article
To identify novel cosmetic ingredients from subtropical plants, we screened 21 parts of 12 plant species collected from the Agricultural Research Institute for Climate located in Jeju Island, the southernmost island of the Korean Peninsula. Subtropical plants were investigated for their total polyphenolic content by using the Folin-Ciocalteu reagen...
Article
In the present study, we investigated the effects of Citrus unshiu flower on regulatory mechanisms of cytokines and nitric oxide (NO) involved in immunological activity of RAW 264.7 macrophages. Our results indicated that ethyl acetate fraction of Citrus unshiu flower (CUF-EA) downregulated LPS-induced nitric oxide (NO) synthase (iNOS) and cyclooxy...
Article
This study investigated the anti-oxidative and anti-inflammatory activity of unripe fruit peel solvent fractions of the native jeju citrus ?Hongkyool? (Citrus tachibana Tanaka) and ?Pyunkyool? (C. tangerina Hort. ex Tanaka). The total polyphenol content and total flavonoid content were highest in the butanol fraction of both ?Hongkyool? (534.4 mg/g...
Article
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In order to test the effectiveness of tangeretin at ameliorating melanoma and melanoma-associated depigmentation, western blotting was used to assess the melanin content of treated melanoma cells. Tangeretin, a 4',5,6,7,8-pentamethoxyflavone, was found to trigger intracellular melanin production in a concentration-dependent manner in B16/F10 murine...
Article
Aquired hypopigmentation disorders include malignancy-induced and postinflammatory hypopigmentation. Recently, sinensetin (5,6,7,3’,4’-pentamethoxyflavone) has been reported to have antiangiogenic and anti-inflammatory effects. To evaluate melanogenic effects of sinensetin in B16/F10 murine melanoma cells under the similar condition of malignancy-i...
Article
The major objective of this study was to investigate the usability as cultivar for processing of new Citrus hybrid `Hamilgam`. We investigated various antioxidant activities, such as DPPH radical, ABTS radical, Hydroxyl radical and Superoxide anion radical effect, along with total polyphenol content and total flavonoid content of Citrus hybrid `Ham...
Article
To investigate the suitability of the green alga Cladophora wrightiana, grown in the southern regions of the Korean Peninsula, as a source of anti-inflammatory agents for human use. Anti-inflammatory effects of the ethyl acetate extracts of C. wrightiana (CWE) were evaluated on lipopolysaccharide (LPS)-treated RAW 264.7 macrophages. CWE were examin...
Article
Citrus, yield of which is the highest among fruits produced in Korea, is extensively consumed for processed food items. The amount of by-products of citrus produced from the processing within a short period after the harvest is tremendous. These by-products are mostly dumped into land or neglected because of cost involved in processing them. The ai...
Article
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Objective: To investigate the anti-inflammatory effects of Jeju seaweeds on macrophage RAW 264.7 cells under lipopolysaccharide (LPS) stimulation. Methods: Ethyl acetate fractions were prepared from five different types of Jeju seaweeds, Dictyopteris divaricata (D. divaricata), Dictyopteris prolifera (D. prolifera), Prionitis cornea (P. cornea),...
Article
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Though many essential oils from citrus peels are claimed to have several medicinal functions, the chemical composition and biological activities of the essential oils of Citrus flowers have not been well described. Therefore, this study intended to investigate the chemical composition and anti-inflammatory potential of essential oils from C. unshiu...
Article
Hypochoeris radicata, an invasive plant species, is a large and growing threat to ecosystem integrity on Jeju Island, a UNESCO World Heritage site. Therefore, research into the utilization of H. radicata is important and urgently required in order to solve this invasive plant problem in Jeju Island. The broader aim of our research is to elucidate t...
Article
This study was designed to analyze the chemical composition of essential oil in 'Shiranuhi' immature fruit and to test their biological activities. 'Shiranuhi' immature essential oils (SIEO) were obtained by steam distillation from fruits collected from Jeju Island and were analyzed using gas chromatograph (GC)-flame ionization detectors (FID) and...
Article
A number of essential oils derived from plants are claimed to have several medicinal functions, including anti-cancer and anti-inflammation effects. However, the chemical composition and biological activities of flower-derived components have not been sufficiently characterized. Therefore, we investigated the composition of essential oils from Hall...
Article
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To investigate the suitability of citrus-press cakes, by-products of the juice industry as a source for the whitening agents for cosmetic industry. Ethylacetate extracts of citrus-press cakes (CCE) were examined for their anti-melanogenic potentials in terms of the inhibition of melanin production and mechanisim of melanogenesis by using Western Bl...
Article
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This study was conducted to identify the anti-melanogenesis constituents from a seaweed Dictyota coriacea (Holmes). Three known compounds, viz. 1,9-dihydroxycrenulide (1), epiloliolide (2) and D-mannitol (3), were isolated from the ethanol extract. The melanin synthesis inhibition activities were evaluated using B16F10 melanoma cells for the isolat...
Article
Development of natural products possessing anti-inflammatory activities has been the focus of research in our laboratory. In this study, the extract from Carpinus turczaninowii branches was studied and their anti-inflammatory constituents were identified. The aqueous ethanol extract was fractionated successively to afford n-hexane, ethyl acetate, n...
Article
Chemical investigation to identify the antioxidative constituents from the stems of a tree Cleyera japonica Thunb. resulted in the isolation of seven compounds: catechin (1), catechin 3-O-alpha-L-rhamnopyranoside (2), epicatechin (3), taxifolin (4), taxifolin 3-O-alpha-L-arabinopyranoside (5), taxifolin 3-O-alpha-L-rhamnopyranoside (6) and proantho...
Article
This study was designed to investigate the mushroom tyrosinase inhibitory activity for the constituents isolated from Neolitsea aciculata. The stems of N. aciculata was extracted with aqueous ethanol and subjected to chromatographic separation, which led to the isolation of 11 compounds: methyl linoleate (1), catechin (2), epicatechin (3), afzelin-...
Article
3-O-p-Coumaroyl-1-(4-hydroxy-3,5-dimethoxyphenyl)-1-O-β-D-gulcopyranosylpropanol (ESQ10) is a naturally occurring phenylpropanoid derivative isolated from Sasa quelpaertensis (Gramineae). In the present study, we discovered that ESQ10 inhibits nitric oxide (NO) and prostaglandin E(2) (PGE(2)) production in lipopolysaccharide (LPS)-stimulated RAW 26...
Article
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This study examined the chemical composition of Neolitsea aciculata essential oil (NAE) and its biological activities. NAE was obtained by hydro-distillation of N. aciculata leaves collected in Jeju Island and analyzed by gas chromatography equipped with a mass spectrometer detector. 1-Dodecen-3-yne (12.5%), calarene (11.5%) and elemol (9.5%) were...
Article
Phytochemical investigation of the branches of Ficus erecta var. sieboldii King resulted in the isolation of eight constituents: p-hydroxybenzoic acid (1), methyl p-hydroxybenzoate (2), vanillic acid (3), methyl vanillate (4), syringic acid (5), β-sitosterol (6), α-amyrin acetate (7), and ethyl linoleate (8). Their chemical structures were identifi...
Article
A number of essential oils from citrus peels are claimed to have biological activities. Citrus peel, called 'Jin-Pi', is used in traditional medicine for digestion, severe cold, and fever. However, the antibacterial activities against skin pathogens and anti-inflammatory effects of the essential oils of Citrus sunki (JinGyul) and Fortunella japonic...
Article
Bioassay-guided investigation of the stems of Maackia fauriei led to the isolation of seven flavonoid constituents, formononetin (1), genistein (2), daidzein (3), texasin (4), tectorigenin (5), odoratin (6) and mirkoin (7). Their structures were elucidated on the basis of spectral studies as well as by comparison with literature data. Tyrosinase in...
Article
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Ni-3at.%W alloy tapes for YBCO coated conductor were fabricated by powder metallurgy process including powder compaction, cold isostatic pressing(CIP), cold rolling and annealing for recrystallization. The Ni-3at.%W tapes annealed in an atmosphere of 96%Ar and 4%H2 mixing gas were characterized by X-ray pole figures, orientation distribution functi...
Article
A cell-based assay system for monitoring COL1A2 [alpha2(I) collagen gene] promoter activity was developed to determine the influence of activated COL1A2 promoter in human dermal fibroblast cells. A pLuc-COL1A2 promoter plasmid that expresses the luciferase reporter gene in response to COL1A2 promoter activity was constructed. The pLuc-COL1A2 promot...
Article
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The currently commercialized cathode material for Li ion batteries such as LiCoO2 exhibited limit to further improve the performance of the batteries, since the employed screen printing method for cathode fabrication is difficult to reduce the thickness and control the microstructure of the oxide layer. In this studies, we have synthesized Li(Ni1-x...
Article
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The effect of microstructural features of A356 alloys on plastic deformation behavior was studied in the present study. To authors' knowledge, the microstructural effect on mechanical properties of A356 alloy has not been well understood even though this alloy system is one of the most widely used alloys for the industrial purpose. Specially, quant...
Article
Nuclear factor-κB (NF-κB) is a critical transcription factor for maximal expression of many of the cytokines that are involved in the pathogenesis of inflammatory diseases. In this study, we found that pinosylvin, a natural stilbenoid that is a component of the pine leaf (Pinus densiflora), significantly inhibited LPS-induced NF-κB activation in a...
Article
Specifically designed PCR primers were applied to amplify a segment of dTDP-glucose synthase gene from six actinomycete strains. About 300-bp or 580-bp DNA fragments were obtained from all the organisms tested. By DNA sequence analysis, seven amplified fragments showed high homology with dTDP-glucose synthase genes that participate in the biosynthe...
Article
This study was done to analyze the changes in smokers' subjectivity about smoking before and after participation in a 5-day hospitalized smoking cessation program. Q-methodology, which provides a scientific method on subjectivity, was applied. Nine types of subjectivity changes about smoking were identified; Type I, Nicotine craving --> Confident o...
Article
The catabolism of branched chain amino acids, especially valine, appears to play an important role in furnishing building blocks for macrolide and polyether antibiotic biosyntheses. To determine the active site residues of ValDH, we previously cloned, partially characterized, and identified the active site (lysine) of Streptomyces albus ValDH. Here...
Article
A gene encoding valine dehydrogenase (Vdh) has been cloned from Streptomyces albus, a salinomycin producer, and expressed in Escherichia coli. The S. albus Vdh is composed of 364 amino acids that showed high homology with several other amino acid dehydrogenases as well as Vdhs from Streptomyces spp. and leucine and phenylalanine dehydrogenases (Ldh...
Article
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A gene encoding glutamate racemase has been cloned from Aquifex pyrophilus, a hyperthermophilic bacterium, and expressed in Escherichia coli. The A. pyrophilus glutamate racemase is composed of 254 amino acids and shows high homology with glutamate racemase from Escherichia coli, Bacillus subtilis, or Lactobacillus brevis. This racemase converts l-...
Article
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Glutamate racemase (MurI) is responsible for the synthesis of D-glutamate, an essential building block of the peptidoglycan layer in bacterial cell walls. The crystal structure of glutamate racemase from Aquifex pyrophilus, determined at 2.3 A resolution, reveals that the enzyme forms a dimer and each monomer consists of two alpha/beta fold domains...
Article
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Glutamate racemase catalyzes the reversible reaction of L-glutamate to D-glutamate, an essential component of the bacterial cell wall. Glutamate racemase from Aquifex pyrophilus has been crystallized by the hanging-drop vapor-diffusion method using polyethylene glycol 6000 as a precipitant. The crystals belong to space group P6122 or P6522 with uni...
Article
Aquifex pyrophilus is one of the hyperthermophilic bacteria that can grow at temperatures up to 95°C. To obtain information about its genomic structure, random sequencing was performed on plasmid libraries containing 0.5–2 kb genomic DNA fragments of A. pyrophilus. Comparison of the obtained sequence tags with known proteins revealed that 123 tags...

Citations

... Generally, hybrid plants are presumed to exhibit differences in major characteristics [24,25] among identical parent hybrid varieties due to genetic changes during the recombination of pollen or seed parent genes [28,40]. Furthermore, the markers identified in this study can also be used to determine whether the major characteristic genes of the domestically bred hybrid variety 'Minihyang' ('Kinokuni' × 'Ootaponkan') [17,51] originated from pollen parents or seed parents. In light of the results of this study and those of a previous study [40], it is possible to establish whether a hybrid mandarin variety used as a pollen parent or seed parent belongs to the Satsuma mandarin lineage, originates from a specific mandarin lineage, is a true hybrid zygotic seedling, or has nucellar embryony originated from a seed parent lineage [18]. ...
... Purified water was provided between evaluations to eliminate any residual taste from the tongue. Each taste intensity was marked on a 15 cm line scale with 0.5 cm anchors, labeled "very weak" on the left and "very strong" on the right [27]. ...
... The amounts of organic acids and nucleotides showed a negative correlation with the accumulated sunshine duration and average temperature in the few days before harvest (Figures 5 and 7). Sugar and citric acid amounts were reduced in tanned mandarin fruits [45]. In this study, the observed decrease in these metabolites correlates with higher sunlight exposure and a larger temperature difference. ...
... Citrus fruits, which are among the most important fruit crops globally [1], have a distinctive flavor profile and have attracted research attention with regard to their potential health benefits, including improved gut health, obesity management, diabetes mitigation, and cardiovascular disease prevention [1][2][3]. These flavor attributes and health-promoting properties are attributed to phytochemicals, including sugars, organic acids, flavonoids, limonoids, and terpenes [4]. The phytochemical profiles of citrus fruits depend on numerous factors, including the variety [5], growing region [6], postharvest treatment [7], storage conditions [8], and environmental growing conditions [2]. ...
... The volatile patterns of the three lemon samples were dominated by terpenoids, which are mostly found in citrus fruits and are essential for plant growth and development (Kim et al., 2022b Thymol has a rosemary-and thyme-like odor (Schreiner et al., 2020) and is used as a flavoring agent (Burdock, 2009;Suntres et al., 2015). Notably, thymol compounds have antimicrobial (Arfa, 2006;Del Nobile et al., 2008) and antioxidant activities (Horvathova et al., 2014) and are safe food preservatives (Beltrán Sanahuja et al., 2021). ...
... Citrus fruits are rich in bioactive substances that are valuable for disease prevention, including vitamin C, citric acid, flavonoids, carotenoids, and pectin, and they are widely consumed as fresh fruits or as processed fruit products such as juice and jam (Lee et al., 2022). Notably, citrus fruits exhibit significant pharmacological value because they contain about 60 different flavonoids known to have antioxidant, anticancer, and antiinflammatory effects. ...
... reticulata 'Unshiu'(CLU)" (Committee, 2020). The different citrus varieties, the active components of the peel, and the medicinal and edible values will be different (Kim et al., 2021;Y. Wang et al., 2017). ...
... Sunburn is damage occurring in the epidermis of fruits caused by a combination of events such as water deficit, salinity, high temperature, and intense solar radiation, expected to be more common due to climate change (Park et al., 2022). This disorder is primarily observed in high tropic areas, and a strategy to mitigate its effects is the application of calcium carbonate nanoparticles (Teixeira et al., 2022), which act as a suppressor and reduce sunburn in pineapple (Fischer et al., 2022). ...
... The current study is in agreement with study done by [32],entitled "Operating room nurses want differentiated education for perioperative competencies based on the clinical ladder" in South Korea and determined that all competences achieved an average score of 3.5 out of 5. Significant variations were seen across the majority of domains in relation to clinical career (p< 0.001). Notably, perioperative abilities were significantly influenced by foundational knowledge and skills (p< 0.001). ...
... Sciatic nerve injury leads to sensory and motor impairment. Nobiletin can inhibit H 2 O 2 -induced sciatic nerve cell toxicity [52]. Nobiletin (50 or 100 μM) can significantly promote synaptic growth of sciatic nerve cells, which is associated with the BDNF-ERK1/2 and AKT pathways [52]. ...