February 2018
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8 Reads
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February 2018
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8 Reads
February 2018
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9 Reads
Document S1. Supplemental Experimental Procedures, Figures S1–S7, and Tables S1–S3
February 2018
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166 Reads
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40 Citations
Stem Cell Reports
Satellite cells are adult muscle stem cells residing in a specialized niche that regulates their homeostasis. How niche-generated signals integrate to regulate gene expression in satellite cell-derived myoblasts is poorly understood. We undertook an unbiased approach to study the effect of the satellite cell niche on satellite cell-derived myoblast transcriptional regulation and identified the tumor suppressor p53 as a key player in the regulation of myoblast quiescence. After activation and proliferation, a subpopulation of myoblasts cultured in the presence of the niche upregulates p53 and fails to differentiate. When satellite cell self-renewal is modeled ex vivo in a reserve cell assay, myoblasts treated with Nutlin-3, which increases p53 levels in the cell, fail to differentiate and instead become quiescent. Since both these Nutlin-3 effects are rescued by small interfering RNA-mediated p53 knockdown, we conclude that a tight control of p53 levels in myoblasts regulates the balance between differentiation and return to quiescence.
May 2017
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2 Reads
... Myogenesis involves muscle stem cells and progenitor cells to proliferate as myoblasts and differentiate into myotubes [29]. Activated satellite cells migrate from an undamaged muscle to a damaged muscle when cells are injured and then fuse to form new muscle fibers or repair damaged fibers together with existing fibers [30,31]. Prior to maturation into myofibrils, C2C12 myoblast cells express MyoD, an early marker for myogenic regulators, and further differentiate into myocytes [32]. ...
February 2018
Stem Cell Reports