Mitsuhiro Hirase's research while affiliated with Kobe University and other places
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Publications (6)
Exosomes are small (30–100 nm) membrane vesicles that serve as regulatory agents for intercellular communication in cancers. Currently, exosomes are detected by immuno‐based assays with appropriate pretreatments like ultracentrifugation and are time consuming (>12 h). We present a novel pretreatment‐free fluorescence‐based sensing platform for inta...
Ein Fluoreszenzsensor für intakte Exosome, der keine Probenvorbehandlung voraussetzt, wurde durch molekulares Prägen und anschließende Modifizierung hergestellt. In ihrer Zuschrift (DOI: 10.1002/ange.201811142) zeigen T. Takeuchi et al., dass durch Anbringen von Antikörpern und molekularen Fluoreszenzreportern ausschließlich in den nanoskopischen B...
A pretreatment‐free fluorescence‐based sensing platform for intact exosomes was prepared by molecular imprinting and post‐imprinting modifications. In their Communication (DOI: 10.1002/anie.201811142), T. Takeuchi et al. show that by introducing antibodies and fluorescent reporter molecules only inside binding nanocavities, highly sensitive detecti...
Exosomes are small (30–100 nm) membrane vesicles serving as regulatory agents for intercellular communication in cancers. Currently, exosomes are detected by immuno‐based assays with appropriate pretreatments like ultracentrifugation and are time consuming (>12 h). We present a novel pretreatment‐free fluorescence‐sensing platform for intact exosom...
α-Synuclein (α-Syn)-positive intracytoplasmic inclusions, known as Lewy bodies, are thought to be involved in the pathogenesis of Lewy body diseases, such as Parkinson’s disease (PD). Although growing evidence suggests that cell-to-cell transmission of α-Syn is associated with the progression of PD and that extracellular α-Syn promotes formation of...
Exosomes play a critical role in cell-to-cell communication by delivering cargo molecules to recipient cells. However, the mechanism underlying the generation of the exosomal multivesicular endosome (MVE) is one of the mysteries in the field of endosome research. Although sphingolipid metabolites such as ceramide and sphingosine 1-phosphate (S1P) a...
Citations
... Therefore, tears carry rich bioinformation from other seemingly unrelated body organs (8). Studies using bulk tear EV have shown this value by estimating their capabilities in discerning the pathological changes of the ocular system [e.g., dry eye (9) and glaucoma (10)] and non-ocular tissues [e.g., breast cancer (11), prostate cancer (12), and multiple sclerosis (13)]. Nevertheless, although most, if not all, of the protein components in tear EVs have been described, the information regarding subset distribution patterns still needs to be determined, especially because the interaction network between tear EV subgroups and human tissues still needs to be seen. ...
... They utilized a histamine-tagged protein G-tagged protein G to anchor a CD9-targeting antibody to the gold sensor surface, as CD9 is abundant on exosome surfaces. After the immobilization of template exosomes from the PC3 prostate cancer cell line on the surface, methacryloyl disulfide groups were attached for postimprinting modifications [55]. ...
... For sensing materials without reactive groups, MGO detection in plasma/ in vitro is far from satisfactory in terms of sensitivity and limit of detection (LOD) due to the lack of matching hostguest interactions (Table S1). Molecular imprinting materials (MIMs) are state-of-the-art sensing materials owing to their designed recognition sites for analytes, such as nucleic acids, proteins, and viruses [14][15][16][17][18]. MIMs are fabricated by immobilising imprinting molecules in the host polymeric materials (e.g., silica matrices) followed by selective removal of imprinting molecules to create recognition sites [19][20][21][22]. ...
... Another way to overcome the surface irregularities of exosomes is to combine the usage of MIPs with specific antibodies and aptamers. Mori et al. designed a molecularly imprinted sensing platform for the detection of prostate cancer-derived exosomes (Fig. 7B) [112]. A His-tagged protein G was used to bind CD9 targeting antibody to the gold surface of the sensor. ...
... Alpha-synuclein (α-syn) is a small 14-kDa protein first discovered by Maroteaux and colleagues in 1988 [1]. In the central nervous system (CNS), α-syn is expressed abundantly in neurons [2], while it is also present in the peripheral nervous system (PNS), gut, muscle, liver, heart, lungs, kidney, and blood cells [3,4]. Since α-syn is enriched in presynaptic terminals and associated with synaptic vesicles, a large number of studies indicates the important role of α-syn in neurotransmission and synaptic plasticity [5][6][7]. ...
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... Ceramide can also be metabolized to produce sphingosine 1-phosphate (S1P), which binds to the S1P receptor on MVBs. The activated S1P receptor promotes the polymerization of microfilaments on endosomal membranes by activating the small G proteins CDC42 and Rac1, which in turn facilitates cargo sorting to ILVs [40][41][42][43]. This process is considered independent of ESCRT. ...
