Michael v. Canstein's research while affiliated with University of Hamburg and other places
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Publications (2)
Mouse fibroblast L-929 cells synchonized by mitotic selection were irradiated during the G1-phase of the cell cycle with a dose of 1000 rad. The rat of DNA synthesis was measured by 3H-thymidine incorporation, and the progression of the cells through the cell cycle was determined using a pulse-cytophotometer. Irradiation caused a decrease in the ra...
Mechanically synchronized cultures of L-cells were treated in various phases of the cell cycle with daunomycin. After incubation for 4 hr at a concentration of 1 μg/ml the percentage of cells in the various phases was determined using an impulse-cytophotometer†. Applying daunomycin in G1 phase produced an arrest of cells in G1 that had not been obs...
Citations
... The same conclusion was also made regarding the number of Ag-positive, i.e., competent, NORs (Fig. 2, Table 1). Taking into account that in L929 fibroblasts, the duration of cell cycle is 20-24 h (König et al. 1975), we approximated that the NOR recovery process required 150-200 cell generations. It is noteworthy to mention that like other researchers, who employ the in situ hybridization and Ag-NOR staining methods in cells with multiple NORs, we cannot exclude that detection of minor rDNA sites remained below the levels provided by the protocols used. ...