Md Rafique Ahmed's research while affiliated with Aligarh Muslim University and other places
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Publications (6)
Present study reports successful in vitro clonal propagation of a potential medicinal plant, Cassia alata using mature nodal explants. Murashige and Skoog (MS) medium supplemented with different concentrations (0.5, 2.5, 5.0, 7.5, 10.0 and 12.5 μM) of 6-benzyladenine (BA), kinetin and 2-isopentenyl adenine (2-iP) singly as well as in combination wi...
The current study reports the encapsulation of nodal segment of V. trifolia L. excised from 2-month-old in vitro-raised cultures for short-term conservation and propagation. The encapsulation of nodal segments was significantly affected by the concentrations of sodium alginate (Na alginate) and calcium chloride (CaCl2 2H2O). The best gel complex us...
An efficient, cyclic, two-step protocol for clonal in vitro regeneration system of an antiallergenic plant, Cassia alata, has been successfully developed. Nodal explants from a 5-year-old tree were cultured on Murashige and Skoog (MS) medium supplemented with various concentrations (1.0, 2.5, 5.0, 7.5, and 10.0 μM) of thidiazuron (TDZ). TDZ (5.0 μM...
The stimulatory effect of thidiazuron (TDZ) has been investigated in shoot multiplication for a simple, efficient, rapid, and commercially applicable regeneration protocol of an important medicinal plant, Cassia alata. Furthermore, the effects of an increased photosynthetic photon flux density (PPFD) on photosynthesis, the functioning of the photos...
The objective of the study was to develop an in vitro shoot regeneration protocol by utilising shoot tips explant from Vitex trifolia L. Shoot tip explants obtained from a 3-year old plant was cultured on Murashige and Skoog (MS) medium supplemented with various concentrations (1.0, 2.5, 5.0, 7.5 or 10.0 A mu M) of thidiazuron (TDZ). The optimal le...
The effect of thidiazuron (TDZ) has been investigated in shoot multiplication for a simple, efficient, rapid, and commercially applicable regeneration protocol of an important medicinal plant, Vitex trifolia. Multiple shoots were induced in nodal explants obtained from a mature tree on Murashige and Skoog (MS) medium supplemented with TDZ in variou...
Citations
... Genetic variations could occur in tissue culture-regenerated plants [79]. The development of somaclonal anomalies between the regenerated plants may limit the effectiveness of the micropropagation protocol [80,81]. Various factors such as genotype, explant type, culture periods, and growth regulator combinations and concentrations may disturb the internal polarity and physiology of the explants [82,83], and could also influence the true-to-type of tissue cultured plants [83,84]. ...
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... Concentrations of 3% for sodium alginate and 100 mM for CaCl 2 were chosen for the production of synthetic seeds of I. pallida species, effectively facilitating the formation of alginate beads coating the plant tissues without negatively impacting the regrowth of somatic embryos. The alginate solution serves as an artificial endosperm [34]. MS medium was employed to create the alginate beads around orris embryos, allowing for the absorption of medium salts, vitamins, and sugar to encourage embryo development into new plantlets. ...
... In vitro propagation of various plant species has been reported using TDZ, one of the most effective plant growth regulator and derivative of phenyl urea (Fiola et al. 1990;Hutchinson et al. 1996;Kumari et al. 2018). Very low concentrations of TDZ may not provide sufficient stimulus to generate optimum response (Ahmed et al. 2014), while prolonged exposure had adverse effects (Ahmad and Anis 2007). High rate of multiplication on a basal media lacking any growth hormones has been documented in TDZ exposed explants (Khan and Anis 2012; Mok et al. 1982;Capelle et al. 1983). ...
... On the other hand, the shoots in micropropagation that are subjected to a biotic stress such as low or high light intensity beyond the optimal level for each plant, lead to notable morphological and physiological changes (Ahmed and Anis 2014b). The adaptation of plants in vitro requires swift adjustments in the function of antioxidant enzymes and compounds, closely correlated with phenotypic and environmental variations. ...
... Plantlets transferred S. Uma et al. from the controlled in vitro environment to ex vitro environment, they undergo a period of acclimatization. This transition introduces various stresses, including changes in temperature, light intensity, humidity, and pathogen exposure (Chakrabarty and Datta, 2008;Varshney and Anis, 2012;Ahmed and Anis, 2014;Ahmad et al., 2022). In response to the factors, plants often enhance their metabolic activities, including chl synthesis and enzyme production, to adapt and survive in the new environment. ...
... In the shoot multiplication phase, TDZ treatment induced the highest number of shoots from nodal explants in A. majus but the treatment failed to elongate the lateral shoots. The efficacy of TDZ in triggering multiple shoot formation has also been reported in Vitex trifolia (Ahmed & Anis, 2012), Physalis peruviana (Yücesan et al., 2015), and Monstera deliciosa (Sivanesan et al., 2023). To elucidate the efficacy of TDZ in multiple shoot formation in A. majus, the interaction of TDZ treatment with the synthesis of related phytohormones and molecular mechanisms should be investigated. ...
