Mauricio Vázquez-Pichardo's research while affiliated with Instituto de Diagnóstico y Referencia Epidemiológicos and other places
What is this page?
This page lists the scientific contributions of an author, who either does not have a ResearchGate profile, or has not yet added these contributions to their profile.
It was automatically created by ResearchGate to create a record of this author's body of work. We create such pages to advance our goal of creating and maintaining the most comprehensive scientific repository possible. In doing so, we process publicly available (personal) data relating to the author as a member of the scientific community.
If you're a ResearchGate member, you can follow this page to keep up with this author's work.
If you are this author, and you don't want us to display this page anymore, please let us know.
It was automatically created by ResearchGate to create a record of this author's body of work. We create such pages to advance our goal of creating and maintaining the most comprehensive scientific repository possible. In doing so, we process publicly available (personal) data relating to the author as a member of the scientific community.
If you're a ResearchGate member, you can follow this page to keep up with this author's work.
If you are this author, and you don't want us to display this page anymore, please let us know.
Publications (20)
In 2014, the chikungunya virus (CHIKV) was detected for the first time in Mexico, the identified strain was the one corresponding to the Asian genotype which was phylogenetically grouped with the strains that circulated in the British Virgin Islands outbreak and was later classified with lineages of Caribbean strains. In three years, 13,569 cases o...
Zika cases have been reported in 29 out of the 32 states of Mexico. Information regarding which mosquito species might be driving Zika virus transmission/maintenance in nature must be regularly updated. From January 2017 to November 2018, mosquitoes were collected indoors and outdoors using the CDC backpack aspirator in urban and semi-urban areas w...
Here, we report for the first time the circulation of dengue virus type 1 (DENV-1) belonging to the lineage IV of genotype V (African American genotype) based on phylogenetic analysis of nucleotide sequences from 10 DENV-1-positive samples obtained in Mexico between 2012 and 2014. Our data revealed that the lineages III and IV of DENV-1 genotype V...
Background: We report on the results of an entomovirological surveillance system of Aedes populations per- formed by the Ministry of Health of the central state of San Luis Potosí, Mexico.
Methods: Indoor adult Aedes aegypti and Aedes albopictus pools collected at San Martín, Tamazunchale, Ciudad Valles, Metlapa, Ebano, Tamuin and Axtla during the...
To assess the possible circulation of Zika virus (ZIKV) prior to the first documented case in Mexico, we reanalyzed the stored samples from the states of Veracruz and Yucatán, which were originally collected to test for dengue (DENV) and chikungunya (CHIKV) but were negative for these viruses despite the symptomatology. The samples were originally...
Zika virus belongs to the genus Flavivirus , and its spread remains an international public health emergency. In this report, we describe the obtainment and molecular characterization of a complete viral genome through the direct metagenomic analysis from saliva from an autochthonous transmission case in Mexico.
We identified 25 autochthonous chikungunya virus cases in Mexico, initially detected by RT-PCR targeting the E1 gene and propagated in C6/36 Aedes albopictus cells, in 2014. To determine the type of virus found, in a previous report, the genomes of 2 CHIKV strains were fully sequenced. Genome sequence analysis revealed that these isolates from Mexi...
The mosquito-borne chikungunya virus, an alphavirus of the Togaviridae family, is responsible for acute polyarthralgia epidemics. Here, we report the complete genome sequences of two chikungunya virus strains, InDRE04 and InDRE51, identified in the Mexican states of Jalisco and Chiapas in 2014. Phylogenetic analysis showed that both strains belong...
In anticipation of this changing landscape, the Mexican Federal Ministry of Health (FMoH), in partnership with the Carlos Slim Health Institute, undertook the development of a national strategy for the introduction of a dengue vaccine. This exercise aimed to establish evidence-based policy recommendations to enable the early adoption of a dengue va...
Here, we describe a transmission event of hepatitis C virus (HCV) among injection drug users. Next-generation sequencing (NGS)
was used to assess the intrahost viral genetic variation. Deep amplicon sequencing of HCV hypervariable region 1 allowed for
a detailed analysis of the structure of the viral population. Establishment of the genetic related...
The use of telaprevir and boceprevir, both protease inhibitors (PI), as part of the specifically targeted antiviral therapy for hepatitis C (STAT-C) has significantly improved sustained virologic response (SVR) rates. However, different clinical studies have also identified several mutations associated with viral resistance to both PIs. In the abse...
Dengue virus (DENV) is the most important arthropod-borne viral infection in humans. Here, the genetic relatedness among autochthonous
DENV Mexican isolates was assessed. Phylogenetic and median-joining network analyses showed that viral strains recovered from
different geographic locations are genetically related and relatively homogeneous, exhibi...
Varicella (chickenpox) exhibits a characteristic epidemiological pattern which is associated with climate. In general, primary infections in tropical regions are comparatively less frequent among children than in temperate regions. This peculiarity regarding varicella-zoster virus (VZV) infection among certain age groups in tropical regions results...
Background. Dengue is a public health priority in Mexico. Since 2008, the dengue diagnostic algorithm for epidemiological and virological surveillance has been improved at InDRE and the public health laboratory network (RLESP) to optimize geographic representation, opportunity, sensitivity and specificity of the produced information. Methods. Dengu...
Background. Dengue is a public health priority in Mexico. Since 2008, the dengue diagnostic algorithm for epidemiological and virologi- cal surveillance has been improved at InDRE and the public health laboratory network (RLESP) to optimize geographic representation, opportunity, sensitivity and specificity of the produced information.
methods. Den...
Evaluation of an "in-house system" for the diagnosis of dengue infection by detection of specific IgM and IgG antibodies showed that 25 out of 34 (73.53%) serum samples were positive for IgM antibodies; 6 (17.64%) were positive for IgG and 3 (8.8%) were negative for both IgM and IgG anti-DENV antibodies. Ten samples from "non-symptomatic" people we...
The prevalence of dengue in Mexico and the participation of the four serotypes make the correct handling of the disease a difficult task. Dengue-infected people will probably present different clinical profiles depending on the dengue serotypes and genotypes. As a consequence, both diagnosis and treatment will become difficult. Administration of pa...
Citations
... Chikungunya virus (CHIKV) is an arbovirus from the Togaviridae family mainly transmitted by Aedes aegypti and Aedes albopictus [1,2]. The acute phase of Chikungunya is characterized by fever, headache, nausea, vomiting, maculopapular lesions, myalgia, and arthralgia [3,4]. The progression to the chronic phase occurs in up to 30% of cases with frequent permanence of joint symptoms, which in some cases can be disabling [5]. ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/11431281233947145-1712170076034_Q64/J-Alberto-Diaz-Quinonez.jpg)
... Meanwhile, A. albopictus has been recorded for 40 years and has the potential to be a significant vector in the Americas (Garcia-Rejon et al. 2021). This is supported by the discovery of dengue and Zika infected populations of A. albopictus (Figueiredo et al. 2010;Sanchez-Rodriguez et al. 2014;Correa-Morales et al. 2019;Alencar et al. 2021). Moreover, A. albopictus has been reported as infected with these arboviruses during outbreaks in Mexico and Brazil (Ibáñez-Bernal et al. 1997;Ricas et al. 2020). ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/829431706816517-1574763337855_Q64/Fabian-Correa-Morales.jpg)
![[object Object]](https://i1.rgstatic.net/ii/profile.image/11431281236227213-1713076972766_Q64/Cassandra-Acosta-2.jpg)
![[object Object]](https://i1.rgstatic.net/ii/profile.image/277951892475905-1443280303860_Q64/Miguel-Moreno-Garcia-2.jpg)
... DENV-1 genotype V is widely distributed in the Americas, and its clades are present in South America, the Caribbean, and Central America (Villabona-Arenas and Zanotto, 2013). This genotype has been circulating in Mexico since the late 1970s, and has experienced several lineage replacements since its introduction, causing a high degree of genetic variability (González-Durán et al., 2018). Our phylogenetic tree of DENV-1 showed that all our sequences grouped in three different clades, within genotype V, together with sequences from Nicaragua. ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/326881980239872-1454946145587_Q64/Elizabeth-Gonzalez-Duran.jpg)
![[object Object]](https://i1.rgstatic.net/ii/profile.image/822004592676864-1572992575404_Q64/Jesus-Miguel-Torres-Flores.jpg)
![[object Object]](https://i1.rgstatic.net/ii/profile.image/751936093581312-1556286943196_Q64/Fabiola-Garces-Ayala.jpg)
![[object Object]](https://i1.rgstatic.net/ii/profile.image/325918296952832-1454716385868_Q64/Jose-Ernesto-Ramirez-Gonzalez.jpg)
... The extent to which Ae. albopictus contributes to transmission of dengue virus in Mexico is unknown. However, the species has been found naturally infected with dengue and Zika virus (Ibáñez-Bernal et al. 1997, Huerta et al. 2018 The species invaded tropical and semiarid/arid areas at different altitudes in Nearctic and Neotropical biogeographical zones (Ortega-Morales et al. 2022). Increased occurrence of Ae. albopictus in Mexico suggested that without the mosquito species Mexico could potentially be at risk of invasion. ...
... Another is that there is an increase in reporting of confirmed dengue cases, which has also been the case in areas such as the Americas from 2000 to 2010 (Guzman et al., 2013). The data identified 2009 and 2011 as epidemic years (Dick et al., 2012;Liao et al., 2015;Vázquez-Pichardo et al., 2011). Serotypes circulating in the years of 2009 and 2011 might have influenced these epidemics (Garcia-Rejon et al., 2011;Sanchez-Casas et al., 2013;VázquezPichardo et al., 2011). ...
... Additionally, the ability of the species Ae. aegypti and Ae. albopictus to transmit ZIKV [18][19][20][21] and CHIKV [22][23][24], is an important factor for the spread of these viruses in the Americas [11,25]. Also, it should be considered that other highly anthropophilic local species may be involved in arbovirus transmission as secondary vectors [26]. ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/1128469551415299-1646059515394_Q64/Pablo-Kuri-Morales.jpg)
... The virulence of different ZIKAV strains could also modulate immunity in vivo. In this regard, we could not sequence the genome of our clinical ZIKAV isolate, but other studies showed that the ZIKAV strains circulating in Mexico at the time when we recruited our cohort belong to an Asian lineage (Díaz-Quiñonez et al. 2016). Indeed, the profile of immune factors produced by monocytes in response to our clinical isolate was similar to the response observed in PBMCs infected with a strain of the Asian lineage (Colavita et al. 2018). ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/1111442266820611-1641999894851_Q64/Edgar-Mendieta-Condado.jpg)
... Finally, for DENV typing, D2 oligonucleotide was replaced with serotypespecific oligonucleotides, and the PCR products were resolved in 4% agarose gels. For ZIKV identification, 5 mg of total RNA was retrotranscribed with random primers (Thermo), and a fragment of 760 bp was amplified in the NS5 region of the gRNA using the oligonucleotides ZikV9113Fwd TTYGAAGCCCTTGGATTCTT and ZikV9872Rev CYCGGCCAATCAGTTCATC and the protocol designed by Dıáz-Quiñonez et al. (Dıáz-Quiñonez et al., 2016). All PCRs were performed using the GoTaq master mix kit (Promega, Madison, WI, USA). ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/11431281112049741-1673293809657_Q64/Noe-Escobar-Escamilla.jpg)
... 10 The first laboratory-confirmed case of ZIKV was reported in the 100 central state of Querétaro, Mexico. 11 Recent studies concluded that low socio-economic status independently increases the risk of arboviral ( DENV , CHIKV and ZIKV ) diseases. While some studies also evaluate the effect of gross domestic product in predicting the prevalence of disease , 105 m ore granular socio-economic indicators like access to education, information and technological infrastructure are suggested as better predict ion factors for disease distribution. ...
... Evolutionary rate is widely recognized as a critical factor influencing the usefulness of any chosen locus [46][47][48]. The standard approach of studying molecular diversification using only a single structural gene of the virus [49,50] has been applied to CHIKV. However, the limited phylogenetic signal generated incongruent phylogenetic hypotheses lacking enough support [51]. ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/277850574868481-1443256147337_Q64/Joanna-Ortiz-Alcantara.jpg)
