Manu Gupta's research while affiliated with Uttar Pradesh Dental College and Research Centre and other places

... While OA can affect any joint, the knee is the most commonly affected, with 10% of men and 13% of women over 60 experiencing symptomatic knee osteoarthritis (KOA) [3]. This prevalence is expected to rise due to increasing life expectancy and the obesity epidemic [4,5]. Presently, nonoperative treatment options include physical therapy, nonsteroidal anti-inflammatory drugs, and intraarticular injections of corticosteroids and hyaluronic acid (HA) [6,7]. ...

... Artificial intelligence and machine learning algorithms have attracted great attention in recent years as a promising, innovative approach to diagnosis (34). According to a machine learning study conducted for the diagnosis of subscapular tendon tears, it was found that MRI alone was successful in predicting subscapularis tears in 85% of patients, and this machine learning increased the accuracy and sensitivity in diagnosis (35). ...

... RC-related disorders pose a great challenge to clinicians, and the lack of an effective gold standard treatment markedly impacts the quality of life of patients, posing a substantial burden on healthcare systems across the world [1][2][3][4][5][6]. In an in vitro study, treatment of tenocytes for 48 h, post-incubation with corticosteroids for 24 h, with allogenic PRP increased the rate of tenocyte proliferation [11]. ...

... Second, EVs are regarded as favorable candidates for cell-free regenerative medicine. 121 Nevertheless, EVs still face several limitations and practical dilemmas in terms of storage, formulation, transportation, liberation, and overall mechanistic understanding. [122][123][124] The establishment of standardized protocols, the implementation of scale-up strategies, and process optimization are essential prerequisites for the future advancement of applications. ...

... 3,4 In July 2022, the World Health Organization declared Mpox as a Public Health Emergency of International Concern. [8][9][10] Mpox has exhibited a concerning global dissemination with an escalating trend. From January 1, 2022 through November 30, 2023, the number of cases surged to over 92,783, documented across 116 countries. ...

... The last decade has seen a significant increase in the use of biologics for MSK regenerative medicine applications. Biologics can be derived from autologous sources, such as platelet-rich plasma (PRP), or allogenic sources, such as perinatal tissue derived formulations (1)(2)(3). In our research topic, "Regenerative Biologics for Musculoskeletal Injuries", three articles highlighted the potential of these biologics for different MSK conditions, and one emphasized the importance of ultrasound guidance to improve injection accuracy. ...

... 17 Remarkably, WJ-MSCs represent a young MSC source with a high proliferation rate, phenotype retention over multiple in vitro passages, lower immunogenicity and tumorigenicity, and greater ex vivo expansion capabilities compared to bone marrow (BM)-derived MSCs. 18 Furthermore, WJ-MSCs have been previously shown to differentiate towards an NPC phenotype, protect NPCs from apoptosis, and induce ECM production with increased disc height and IVD hydration in vivo. 19 Interestingly, EVs harvested by this MSC subpopulation have yielded promising results in several other preclinical models of inflammatory and degenerative disorders (e.g., Parkinson's disease, osteoarthritis, etc.), 20,21 although no previous study has specifically investigated their potential regenerative effects on IDD. ...

... These properties make them ideal for therapeutic purposes and regenerative medicine. EVs have shown promise as a therapeutic agent in regenerative medicine, tissue engineering and immunotherapy due to their characteristics that stimulate tissue regeneration and enhance immunomodulatory properties [12][13][14]. The different cell type affect the contents and functions of EVs [15]. ...

... EVs are not only very heterogeneous but have also been reported to show different outcomes. MSCs cultured in serumstarved media resemble stressed culture conditions, and can secrete tumor encouraging EVs: thus, prudence is recommended when utilizing such EVs for regenerative medicine applications [87,88]. In addition, a few clinical studies have been stopped without publication. ...

... In contrast, stem cell therapy often faces problems related to immune rejection and immunogenicity. The immunomodulatory potential of EVs allows them to bypass the immunological barriers associated with stem cell transplantation, thus increasing their therapeutic efficacy and safety (Natasha et al. 2014;Gupta et al. 2020). ...