Man-Rong Huang's research while affiliated with Academia Sinica and other places

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Publications (3)


Overlooked taxa ofStereocaulon (Stereocaulaceae, Lecanorales) in China
  • Article

May 2006

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21 Reads

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4 Citations

Nova Hedwigia

Man-Rong Wei Huang

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Jiang-Chun Wei

Twelve species and two varieties of Stereocaulon are reported for the first time from China, viz. Stereocaulon alpestre, St. botryosum, St. coniophyllum, St. depreaultii, St. exutum, St. foliolosum var. botryophorum, St. graminosum, St. himalayense, St. intermedium, St. myriocarpum var. altaicum, St. prostratum, St. rivulorum, St. saxatile and St. sterile. Descriptions and remarks are given.

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FIG. 1. Sketch maps of collecting sites. A, five localities in China ( N Mount Wuling, Hebei;. Mount Arxan, Motianling and Mount Jiguan, Inner Mongol; m Mount Tuding, Jilin; X Jimsar, Xinjinag; & Hanas and Guanyuting, Xinjiang). B and C are the loci at 2100 m and 1700 m elevations respectively. Each dot represents a single mat. Samples were taken from every mat. Samples' serial numbers indicate their positions. For example, R. chrysoleuca111 was collected from 1-1 mat in B locus (2100 m), and R. chrysoleuca246 was collected from 2-4 mat in C locus (1700 m).
FIG. 2. The NJ tree inferred from ITS data. The individuals that are marked with same tag were taken from the same area and these tags are corresponding to those in FIG. 1 (both ''#'' and '' N '' indicate that samples were taken from Mount Wuling, and ''#'' refers to samples with gray thallus). The numbers in each node represent bootstrap support values, the absence of a number indicating ,50% bootstrap support. (HB 5 Hebei, NM 5 Nei Mongol, XJ 5 Xinjiang, JL 5 Jilin). The morphological types, the chemotypes and the insertion distribution patterns are listed at the right columns.
FIG. 3. Insertions in the different positions of the SSU rDNA in R. chrysoleuca. An inverted triangle indicates the presence of an intron in a particular position. The number below the horizontal line gives an indication of the nucleotide's position (according to Saccharomyces cerevisiae with accession number Z75578). The region which was not sequenced is shown with the broken line. 
A study of the genetic variability of Rhizoplaca chrysoleuca using DNA sequences and secondary metabolic substances
  • Article
  • Full-text available

January 2006

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70 Reads

·

23 Citations

Mycologia

Mycologia

Specimens of Rhizoplaca chrysoleuca from Mount Wuling can be divided into two distinct groups based on obvious differences in morphological characters. Here we investigated 26 specimens of R. chrysoleuca from Mount Wuling, 10 specimens of this species from other areas and seven specimens of other Rhizoplaca species by analyzing morphology, chemistry and genetics. Nine chemotypes were detected among the specimens of R. chrysoleuca from Mount Wuling, and five of them were reported for the first time. Based on the ITS phylogenetic analysis, the chemotypes and the insertion distribution patterns in SSU rDNA, the samples of R. chrysoleuca from Mount Wuling were grouped in two distinct clades corresponding to two phenotypic groups and no gene flow was detected between these two groups. Our results establish all individuals of Rhizoplaca chrysoleuca are conspecific although some populations have been isolated on Mount Wuling, indicating that they are in the process of speciation. Our study also reveals that the relationships between genotypes and chemotypes are complicated and should be avoided, and we instead recommend using single individuals or few individuals from the same site to represent the population or whole species in systematics study. The results also indicate that Rhizoplaca chrysoleuca might provide a good model for studying the speciation of saxicolous lichenized fungi.

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FIG. 1. Sketch maps of collecting sites. A, five localities in China ( N Mount Wuling, Hebei;. Mount Arxan, Motianling and Mount Jiguan, Inner Mongol; m Mount Tuding, Jilin; X Jimsar, Xinjinag; & Hanas and Guanyuting, Xinjiang). B and C are the loci at 2100 m and 1700 m elevations respectively. Each dot represents a single mat. Samples were taken from every mat. Samples' serial numbers indicate their positions. For example, R. chrysoleuca111 was collected from 1-1 mat in B locus (2100 m), and R. chrysoleuca246 was collected from 2-4 mat in C locus (1700 m).
FIG. 2. The NJ tree inferred from ITS data. The individuals that are marked with same tag were taken from the same area and these tags are corresponding to those in FIG. 1 (both ''#'' and '' N '' indicate that samples were taken from Mount Wuling, and ''#'' refers to samples with gray thallus). The numbers in each node represent bootstrap support values, the absence of a number indicating ,50% bootstrap support. (HB 5 Hebei, NM 5 Nei Mongol, XJ 5 Xinjiang, JL 5 Jilin). The morphological types, the chemotypes and the insertion distribution patterns are listed at the right columns.
FIG. 3. Insertions in the different positions of the SSU rDNA in R. chrysoleuca. An inverted triangle indicates the presence of an intron in a particular position. The number below the horizontal line gives an indication of the nucleotide's position (according to Saccharomyces cerevisiae with accession number Z75578). The region which was not sequenced is shown with the broken line.  
A study of the genetic variability of Rhizoblaca chrysoleuca using DNA sequences and secondary metabolic substances

January 2006

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105 Reads

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26 Citations

Mycologia

Mycologia

Specimens of Rhizoplaca chrysoleuca from Mount Wuling can be divided into two distinct groups based on obvious differences in morphological characters. Here we investigated 26 specimens of R. chrysoleuca from Mount Wuling, 10 specimens of this species from other areas and seven specimens of other Rhizoplaca species by analyzing morphology, chemistry and genetics. Nine chemotypes were detected among the specimens of R. chrysoleuca from Mount Wuling, and five of them were reported for the first time. Based on the ITS phylogenetic analysis, the chemotypes and the insertion distribution patterns in SSU rDNA, the samples of R. chrysoleuca from Mount Wuling were grouped in two distinct clades corresponding to two phenotypic groups and no gene flow was detected between these two groups. Our results establish all individuals of Rhizoplaca chrysoleuca are conspecific although some populations have been isolated on Mount Wuling, indicating that they are in the process of speciation. Our study also reveals that the relationships between genotypes and chemotypes are complicated and should be avoided, and we instead recommend using single individuals or few individuals from the same site to represent the population or whole species in systematics study. The results also indicate that Rhizoplaca chrysoleuca might provide a good model for studying the speciation of saxicolous lichenized fungi.

Citations (3)


... Zopf, R. melanophthalma, R. phaedrophthalma and R. subdiscrepans (Nyl.) R. Sant (Zhou et al. 2006;Leavitt et al. 2011Leavitt et al. , 2013aLeavitt et al. , 2016Szczepańska et al. 2020). Five new species were described in the R. melanophthalma-complex, based on molecular phylogenetic results (Leavitt et al. 2013b). ...

Reference:

Two new species of Rhizoplaca (Lecanoraceae) from Southwest China
A study of the genetic variability of Rhizoplaca chrysoleuca using DNA sequences and secondary metabolic substances
Mycologia

Mycologia

... This species can be characterized by its suberect, 2-3 cm tall thallus that is K+ pale yellow (atranorin) and produces of lobaric acid, moderately to very dense cushions of cylindrical, caespitose pseudopodetia completely lacking tomentum, numerous phyllocladia mostly flat, grain-like, or warty to spherical or compound warty, usually 0.1-0.2 mm in diameter and mainly present in the upper parts of the pseudopodetia, and numerous sessile, spherical grey-brown botryose cephalodia (Huang & Wei 2006). ...

Overlooked taxa ofStereocaulon (Stereocaulaceae, Lecanorales) in China
  • Citing Article
  • May 2006

Nova Hedwigia

... Total DNA from mycobionts and photobionts was extracted using a modified CTAB method (Zhou et al., 2006). The mycobiont ITS segments were then amplified using the fungal specific primer A d v a n c e P u b l i c a t i o n Table 1. ...

A study of the genetic variability of Rhizoblaca chrysoleuca using DNA sequences and secondary metabolic substances
Mycologia

Mycologia