M. Kaminski's research while affiliated with Institute of Physical Chemistry, Polish Academy of Science and other places
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Publications 0
If plant cells and organs are to survive exposure to liquid nitrogen, intracellular crystallization must be avoided. This implies preliminary dehydration of the cells before quenching in liquid nitrogen. Three main procedures have been successively proposed to ensure cryopreservation of cells and organs. In conventional procedures, dehydration of t...
After encapsulation in alginate beads and 18 h of preculture with 0.3M sucrose, somatic embryos of carrot were resistant to dehydration and subsequent cooling in liquid nitrogen. Thermograms displayed by differential scanning calorimetry proved that tolerance to cooling in liquid nitrogen of trapped somatic embryos involved a vitrification process...
Citations
... Anther culture with a large percentage of homodiploids may provide an opportunity of finding homozygous genotypes without inbreeding depression and with a good combining ability (Anderson 1984, Hagnefelt 1988, Hu et al. 1993. By means of somatic embryogenesis and the production of artificial seeds, the development of maintainer lines could, potentially, be substituted (Kitto and Janick 1985, Kamada et al. 1989, Dereuddre et al. 1992, Liu et al. 1992, Wake et al. 1992). In Germany, Schuphan (1942) was the first to recommend breeding carrots with a high level of /i-carotene. ...
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... Based on the success of En-de and the development of PVS2 for plant cryopreservation, an En-vi method was developed for cryopreservation of carnation (Diathus caryophyllus L.) shoot tips [135]. This method was first tested in potato by Hirai & Sakai [107], combining the advantages of the easy manipulation of encapsulated explants and dehydration by vitrification solutions. ...
... This method includes similar application with simple dehydration process; however, the main difference from simple dehydration is usage of encapsulated shoot tips. Explants encapsulated in calcium alginate beads desiccates in a laminar airflow cabinet or with activated silica gel for reducing water content, and then they are fast immersed directly in liquid nitrogen [53]. This technique has been used for shoot tips of many species from tropical and subtropical origin as well as to cell suspensions and somatic embryos of several species [54]. ...
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... Therefore, we suggest the Tg value of −67.5 • C of the air-dehydrated alginate beads [63] was influenced by the Tg of the 0.71 g g −1 Suc solution with a corresponding water content of 0.4 g g −1 . This supports a former finding [65] that the minimal Suc concentration for a glass transition without water crystallization occurred at 70% (w/w). We assume that Suc solution acts as a single CPA in the encapsulation-dehydration method due to air dehydration of 0.75 M sucrose to 2.83 M (0.71 g g −1 ). ...
