Lisa M. D'Ambrosio's research while affiliated with University of Toronto and other places
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Publications (12)
The establishment, maintenance, and dissolution of sister chromatid cohesion are sequentially coordinated during the cell cycle to ensure faithful chromosome transmission. This cell-cycle-dependent regulation of cohesion is mediated, in part, by distinct posttranslational modifications of cohesin, a protein complex consisting of the Smc1-Smc3 ATPas...
Silencing of Sec16A and p115 disrupts TRAF3 localization. (A) HeLa cells were transfected with 40 nM nonsilencing RNA duplexes (panels 1 and 3) or 40 nM siRNA duplexes that specifically target Sec16A (panels 2 and 4). At 72 h post-transfection, the cells were stained for endogenous GM130, Sec16A, TRAF3, and the nucleus (DAPI). Arrows in panel 2 ind...
The TRAF3 interactome network. (A) AP-MS data with ≥0.5 AvgP SAINT value. Indicated baits and prey (HUGO gene names; USO1 is the gene name for p115) are listed, alongside the accession number (protein NCBI gi) for the prey, and SAINT output data. Columns are as follows («|» is a delimiter for biological replicates): «IP» are unique identifiers for...
Overexpression of p115 or Sec16A in highly transfectable cell lines inhibited TRAF3-dependent transcriptional activation as depletion of Sec16A or p115. (A–C) 293T cells were co-transfected with pGL3-IFNβ luciferase reporter gene, plus increasing amounts of indicated plasmids along with 15 ng of His-TRIF (A), 15 ng of FLAG-ΔRIGi (B) or 15 ng of FLA...
Microtubule depolarization affects the perinuclear localization of TRAF3. HeLa cells were transfected with FLAG-TRAF3 (panels 1, 2 and 4) or FLAG-TRAF3 and Myc-p115 (panels 3 and 5) and treated with dimethylsulfoxyde (panel 1), 5 µg/ml of nocodazole for 2 h at 37°C (panels 2 and 3) or 5 µg/ml of BFA for 1 h at 37°C (panels 4 and 5) before confocal...
Selective colocalization of TRAF3 with components of the ER-to-Golgi vesicular pathway. (A) Confocal microscopy analysis of HeLa cells transfected with FLAG-tagged TRAF2 (panel 1), TRAF3 (panel 2) or TRAF6 (panel 3). The Golgi apparatus was labeled with an anti-GM130 antibody. (B) Colocalization of FLAG-TRAF2 (panel 1), FLAG-TRAF3 (panel 2) or FLAG...
COPI/COPII-vesicular retention of the TRAF3-AKKFF mutant affects its extraction efficiency as well as interaction with MAVS. 293T cells were co-transfected with Myc-MAVS and the indicated FLAG-TRAF3 constructs (wtTRAF3 or TRAF3-AKKFF). 24 h post-transfection, whole cell extracts were prepared using 1% Triton X-100 or RIPA lysis buffers as indicated...
Mild expression of Sec16A and p115 in Hec1B cells increases activation of ISG56 and NF-κB promoter. (A–C) Hec1B cells were co-transfected with pGL3-ISG56-luciferase reporter gene, 125 ng of empty vector, Myc-p115 or Sec16A and 15 ng of His-TRIF (A), 15 ng of FLAG-MAVS (B) or 100 ng of FLAG-TBK1 (C). (D–F) Hec1B cells were co-transfected with pGL3-N...
Tumor Necrosis Factor receptor-associated factor-3 (TRAF3) is a central mediator important for inducing type I interferon (IFN) production in response to intracellular double-stranded RNA (dsRNA). Here, we report the identification of Sec16A and p115, two proteins of the ER-to-Golgi vesicular transport system, as novel components of the TRAF3 inter...
The serine/threonine protein phosphatases are targeted to specific subcellular locations and substrates in part via interactions with a wide variety of regulatory proteins. Understanding these interactions is thus critical to understanding phosphatase function. Using an iterative affinity purification/mass spectrometry approach, we generated a high...
Protein serine/threonine phosphatase 4 (PP4c) is an essential polypeptide involved in critical cellular processes such as microtubule growth and organization, DNA damage checkpoint recovery, apoptosis, and tumor necrosis factor alpha signaling. Like other phosphatases of the PP2A family, PP4c interacts with regulatory proteins, which specify substr...
Citations
... It has been suggested that acetylation suppresses the ATPase activity of the SMCs and locks cohesin into a protethering conformation . Cohesion is maintained during the G2 phase of the cell cycle as Pds5 protects Scc1 from ubiquitination and degradation (D'Ambrosio and Lavoie, 2014;Hartman et al., 2000;Panizza et al., 2000). When cells enter mitosis, cohesin is gradually released and removed from chromosomes. ...
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... Hence, the background contaminants need to be consistent across all purification procedures to enable effective control experiments. AP-MS has been extensively used in diverse biological research fields, such as the investigation of innate immune signaling pathways and host-pathogen interactions (DeBlasio et al. 2021;Gillen and Nita-Lazar 2017;Morwitzer et al. 2019;Terracciano et al. 2021;van Zuylen et al. 2012). This method allowed discoveries of new interactors and regulatory mechanisms involved in innate immune signaling. ...
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... Key upstream elements in connection with angiogenesis include: (A) Growth factors and receptors such as VEGF, Angiopoetin-2, epidermal growth factor receptor (EGFR), G protein-coupled receptors (GPCRs), TGFR, and Wingless/Integrated (Wnt) signaling; (B) Cell polarity and adhesion components: Crumbs homolog 1 (CRB1), Kibra, merlin, cadherins, as well as members of the angiomotin family (angiomotin-like 1, angiomotin-like 2); (C) Mechanical forces affecting cell geometry. An important regulator of Hippo signal transduction is the STRN-interacting phosphatase and kinase (STRIPAK) complex [169]. The STRIPAK complex consist of the PP2A AC dimer with a STRN-family regulatory subunit (B"') acting as a scaffold for the complex [170,171], several kinases and different scaffolding proteins such as calmodulin (CaM) [172], Mob3, the germinal center kinase III (GCKIII) such as Serine/Threonine Kinase (STK) 24, STK25, MST4, misshapen like kinase 1 (MINK1) [173], family with sequence similarity 40 (FAM40) proteins, and cerebral cavernous malformations 3 protein (CCM3) [174]. ...
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... Protein phosphatase 4, catalytic subunit (PPP4C) plays a role in several cellular processes, such as microtubule growth and organization, DNA damage checkpoint recovery, apoptosis, and TNF-alpha signalling (Chen et al., 2008). However, its role in the context of 16p11.2 ...
