Lin Liu's research while affiliated with Ocean University of China and other places
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Publications (3)
Helicobacter pylori (H. pylori) is recognized as a pathogen associated with several gastrointestinal diseases. The current treatments exhibit numerous drawbacks, including antibiotic resistance. H. pylori can adhere to and colonize the gastric mucosa through H. pylori adhesin A (HpaA), and antibodies against HpaA may be an effective therapeutic app...
Introduction
O-GlcNAcylation is a type of reversible post-translational modification on Ser/Thr residues of intracellular proteins in eukaryotic cells, which is generated by the sole O-GlcNAc transferase (OGT) and removed by O-GlcNAcase (OGA). Thousands of proteins, that are involved in various physiological and pathological processes, have been fo...
Objective To prepare rabbit polyclonal antibody specifically against human glutamine fructose-6-phosphate amidotransferase 1(GFPT1). Methods The protein sequences of GFPT1 and its highly homologous isozyme GFPT2 were compared. Two peptides for the specific sequence of GFPT1 were designed and synthesized. New Zealand rabbits were immunized by peptid...
Citations
... However, the specificity of IgY was comparable with monoclonal antibodies from mouse [12]. scFv possesses the characteristics of IgY as well as its remarkable characteristics, such as easy genetic manipulation to improve further affinity, large-scale preparation with E. coli or yeast expression systems, and batch-to-batch consistency [22][23][24]. In addition, the development of phage display, a cost-effective method compared to hybridoma that avoided the need for repeatedly immunizing chickens [25], made the scFv easy to obtain and enhanced the utility of chicken antibodies. ...
... Xi et al., obtained three vNARs against OGT proteins (2D9, 3F7, and 4G2) from an immune phage display-derived vNAR library [160]. The affinity determination of these three anti-OGT vNARs to their corresponding OGT antigens was assessed through ELISA and plasmon resonance [160]. ...
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