L. T. Volova's research while affiliated with Samara State Medical University and other places
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Publications (89)
Актуальность. Рецессия десны — ак- туальная и остро стоящая проблема, имеющая высокую частоту встречаемости как в России и СНГ, так и мировом масштабе в различных воз- растных и социально-демографических группах. В обзоре даны ключевые представления об этиоло- гии, патогенезе, эпидемиологии и классификации рецессии десны с учётом различных возрастн...
Aim. To develop an in vitro method for assessing the activity of the inflammasome under conditions of hyperuricemic stimulation of inflammatory interleukins. Material and methods. Whole blood cells of donors diluted with RPMI were cultured in vitro in the presence of different concentrations of uric acid. The production of cytokines in the cell gro...
Based on primary human cell cultures obtained from tissues of juvenile donors, bioassays to assess in vitro the biological activity of TNF-α and IL-17A inhibitors have been developed. The fibrotest-bioassay included the quantification of proinflammatory cytokines MCP-1, IL-6 and IL-8 in human foreskin fibroblasts (HFF) stimulated with TNF-α and IL-...
The type I collagen was studied in samples of two types of osteoplastic materials produced in the Biotech Research Institute of the Samara State Medical University using immunoblotting. The demineralized samples used in the work were compact bone powder and crushed material of human cancellous bone tissue. Collagen and its polypeptides were separat...
Relevance . Gum recession is a pathology often encountered both in Russia and worldwide. Modern surgical methods allow for the complete elimination of recession signs when adequately choosing strategy, tactics, methodology and surgical treatment protocol; for complication prevention and stable long-term outcome. Autograft and allogeneic dura mater...
Objective. Gingival recession is a common pathology in Russia and worldwide. The modern techniques of the surgical treatment allow completely eliminating the signs of recession with the adequate choice of the surgical treatment strategy, tactics, technique and surgical protocol, provide the absence of complications and stable results in the long te...
Рецессии десны в процессе или после ортодонтического лечения несъемной техникой – часто встречающаяся патология (65,0-86,7%), обычно в области фронтальной группы зубов, особенно при выдвижении корпусно по дуге в область вне костной массы или при наклоне зуба вестибулярно более пяти градусов по оси (35%). Рецессии до ортодонтического лечения усугубл...
Есть эффективные и безопасные методы лечения, предлагающие практически полное устранение одиночных и множественных рецессий десны. Наиболее распространенным методом лечения рецессии зубов является хирургическое лечение с применением аутотрансплантата.
Рецессии десны - часто встречающаяся патология России и в мире. Современные методики хирургического лечения позволяют полностью устранить признаки рецессий при адекватном выборе стратегии, тактики, методики и хирургического протокола лечения, обеспечить отсутствие осложнений и стабильный результат в долгосрочной перспективе. Применение аутотранспла...
Хирургическое лечение множественных рецессий десны с использованием аллогенной твердой мозговой оболочки «Лиопласт» (Самара) (Лабораторное, Гистологическое, Клиническое, Рентгенологическое исследования)
SURGICAL TREATMENT OF MULTIPLE GUM RECESSIONS USING ALLOGENEIC DURATA LYOPLAST (SAMARA) (Laboratory,histological, clinical, x-ray examination)
Relevance. Gum recessions during or after orthodontic treatment with non-removable technique are a common pathology (65.0-86.7%): usually in the area of the frontal group of teeth; especially when extending the body in an arc to the area outside the bone mass or when the tooth is tilted vestibularly more than five degrees along the axis (35%). Rece...
Актуальность. Рецессии десны в процессе или после ортодонтического лечения не- съёмной техникой - часто встречающаяся патология (65,0-86,7%): обычно в области
фронтальной группы зубов; особенно при выдвижении корпусно по дуге в область вне костной массы или при наклоне зуба вестибулярно более пяти градусов по оси (35%). Рецессии до ортодонтического...
Relevance . Gingival recessions are frequent during or after orthodontic treatment with fixed appliances (65.0-86.7%). They are usually observed in anterior teeth, especially in teeth bodily movements along an archwire outside the bone or in buccal/ labial tipping more than five degrees along the axis (35%). Pre-existing recessions worsen during or...
Recently, research on the use of pulsed magnetic fields (PMF) of high intensity in biology and medicine has been expanding. To understand the morphological changes that occur in a cell under the influence of a PMF, the article considers the factors of such an impact: magnetic, mechanical and thermal. The relationship between the structure of the ce...
Cell-based bioassays have been developed to assess the biological activity of TNF-α and IL-17A inhibitors based on primary human cell cultures obtained from tissues of juvenile donors. The Fibro-bioassay is based on the analysis of IL-17A dose-dependent production of inflammatory cytokines synthesized by human foreskin fibroblasts (HFF). Using this...
Start The experiments in evaluation of hard tissues using the Raman spectroscopy method have been made. Spectral differences between the tooth enamel and dentin before and after the in-office whitening procedure were found as a result of the work. It was shown with the use of the Raman spectroscopy method that the whitening process causes changes o...
The results of the research of enamel of periodontitis-affected teeth before and after open curettage are presented in the work. The Raman spectroscopy method was used as an evaluation method of curettage influence on tooth enamel. Chemometric analysis of the Raman spectra of tooth enamel before and after curettage was made. Spectral changes of too...
The experiments in optical evaluation of biomaterials taken from juvenile dentin have been made using the Raman spectroscopy method. It was shown that juvenile teeth lose their mineral components and preserve organic components in the process of demineralization with the use of hydrochloric acid.
The results of the research of bone mineral component (BMC) composition using the Raman spectroscopy method are presented in the work. The subjects of the research were the groups of BMC samples made of compact bone tissue of cows using “Lyoplast”® technology where vacuum delipidation of initial material was replaced by the flow one by washing it o...
The results of an expanded spectral analysis of donor dentin materials using Raman spectroscopy are presented. Mathematical methods for the enhancement of the resolution of spectral profiles and chemometric analysis for assessing the composition of biomaterials are used. Criteria for the noninvasive assessment of dentin materials using their spectr...
Relevance . Gingival recession is the apical migration of the gingival tissues associated with the exposure of the roots and alveolar bone loss. The prevalence of single recessions in people over 18 years old is 86.7% whereas multiple recessions, i.e. where all teeth are affected, amount up to 28.6%. High prevalence of the pathology necessitates im...
V.N. Germanova1,2, E.V. Karlova1,2, L.T. Volova1, N.N. Sarbaeva3, I.F. Nefedova1, M.V. Radaykina2
1Samara State Medical University, Samara, Russian Federation
2Eroshevskiy Eye Hospital, Samara, Russian Federation
3REAVIZ Medical University, Samara, Russian Federation
Background: anti-inflammatory and antiproliferative agents are among tools to...
To achieve greater clinical relevance of the newly discovered compounds, modern drug discovery requires disease-targeted assays based on human cells. The specific aim of this study was to design and develop a new cell-based assay for screening of compounds with IL-17A inhibitory activity. Human foreskin fibroblasts (HFF) were treated with IL-17A al...
The known negative consequences of space flights on the cosmonaut’s bone system have long been established and require the development of preventive measures in order to provide the possibility of subsequent prolonged space missions. However, the mechanisms underlying these changes in bone tissue occurring in microgravity are still unknown. Another...
The aim of the review is to learn about the areas of application of the Raman spectroscopy in medicine, particularly in dentistry. The method is widely used in biology, medicine, pharmacy, forensic science, gemology, food industry and other industries. The main advantages of Raman spectroscopy are no need for sample preparation and small amounts of...
Using the method of Raman spectroscopy the study of bone tissue of rats has been made in this work after hyperthermia and injection of allogenic hydroxyapatite. It has been established that the process of thermal treatment of bone material up to 70°C for 15 minutes allows preserving organic components of biomaterials
In the context of modern drug discovery, there is an obvious advantage to designing phenotypic bioassays based on human disease-relevant cells that express disease-relevant markers. The specific aim of the study was to develop a convenient and reliable method for screening compounds with Tumor Necrosis Factor-alpha (TNF-α) inhibitory activity. This...
The results of experimental studies of bone tissue in models of osteoporosis are presented, and the effectiveness of the correction of this pathological condition using allogeneic hydroxyapatite is evaluated by Raman spectroscopy. During the studies, Raman spectra of the cancellous and cortical bone tissues were obtained and the spectra were deconv...
The influence of pulsed magnetic fields (PMF) with different values of field intensity H, discharge current frequency f and a number of pulses n on dermal fibroblasts was studied in vitro. A specialized pulse-magnetic installation «PMI-Bio» and a measuring stand for creating and monitoring the specified parameters of the PMF were created. Four seri...
Research of component composition of bioimplants for gum recession treatment using the Raman spectroscopy method is presented in this paper. Spectral assessment of the component composition of hard palate bioimplants prepared by the «Lioplast» technology before and after lyophilisation is carried out. Expanded component analysis of the researched o...
The results of Raman spectroscopy to evaluate alternative sources for donor bone implants using the “Lioplast” technology are presented. It is shown that Raman spectroscopy can be used to assess the component composition of the surface of bone implants during their treatment. It has been found that major differences were at Raman bands 1448, 1735 (...
During the work, experimental studies of spongy bone tissue of animals after ovariectomy and evaluation of its regeneration by allogeneic hydroxyapatite (HAP) by Raman spectroscopy were carried out. Also deconvolution of Raman spectra of the studied samples was carried out. Coefficients allow to evaluate the efficiency of HAP for the correction of...
The results of an advanced spectral analysis of human donor bone grafts obtained using Raman spectroscopy are presented. Mathematical methods were applied to improve the resolution of spectral profiles, and chemometric principal component analysis was used to evaluate the organic components of the grafts. The results were compared to the proteomic...
This paper presents the results of a comparative spectral estimate of the surface components of implant samples based on cerebral dura mater fabricated by the Lyoplast technology, with and without ultrasound treatment. Raman spectroscopy is chosen as the main test method. These studies make it possible to propose criteria that allow the relative co...
Aim:
To develop the technology of manufacturing of reconstructive allogenic bone graft with individual parameters by using of digital prototyping and modeling.
Material and methods:
Allogeneic cadaveric bone blocks were used as material. The following methods were used to develop a manufacturing technology for personalized allogenic reconstructi...
The results of application of the Raman spectroscopy method for evaluation of porosity influence on the process of bone fabric demineralization and for evaluation of component composition of bone grafts with different porosity in the process of their treatment are presented. The comparison has shown the difference in intensities of spectral lines o...
The results of comparative spectral evaluation of surface component composition of dura mater samples made by "Lioplast" method using Raman spectroscopy are shown. The coefficients were implied and the two-dimensional analysis, showing that the treatment preserves the basic components and removes DNA/RNA, improving the quality of material, providin...
The results of application of the Raman spectroscopy method for evaluation of porosity influence on the process of bone fabric demineralization and for evaluation of component composition of bone grafts with different porosity in the process of their treatment are presented. The comparison has shown the difference in intensities of spectral lines o...
The results of the experimental research of the osteoporotic bone tissue and the Raman spectroscopy evaluation of its prevention using allogenic hydroxyapatite during resorption are presented in this work. The Raman spectra were obtained and their deconvolution and comparative analysis was made in this work. The coefficients allowing evaluating the...
The results of the study of synovial fluid (SF) of patients with knee joint osteoarthritis (OA). The optical analysis of the SF samples, harvested during exploratory punctures of knee joints of patients suffering the osteoarthrosis in different stages using the standard method, was made. A certain component composition of surface of SF samples that...
The results of application of the Raman spectroscopy method for estimating alternative sources for the production of bone spongy implants using the "Lioplast" technology, namely, the femoral head heads resected in the operation of hip replacement surgery, are presented. It is shown that Raman spectroscopy can be used to assess the component composi...
There are presented the results of the application of Raman-scattering spectroscopy method (RS) for the qualitative analysis of rams' heart valves surfaces before and during their decellularization. While analyzing RS spectra, it was found that basic differences appear at wave numbers 812 cm⁻¹, 1062 cm⁻¹, 1340 cm⁻¹ and 1440 cm⁻¹, corresponding to p...
Способ хирургического лечения множественных рецессий десны, включающий деэпителизацию анатомических сосочков, мобилизацию слизисто-надкостичного лоскута, обработку поверхности корней, забор пластического материала и его фиксацию к деэпителизированным анатомическим сосочкам; фиксацию поверх пластического материала ротированного и коронально смещенно...
This paper presents the results of Raman spectroscopy studies of 59 samples of hydroxyapatite produced from different types of bone tissue of a ram, cow, Turkey, duck, and goose by isolation from solution during demineralization. The features of the Raman spectra of hydroxyapatite powders obtained from cancellous bone tissue with different degrees...
Experimental data on the estimation of the qualitative composition of heart valves and their tissue bio-matrices via Raman scattering spectroscopy are presented. The analysis of the Raman spectra allowed identification of the main bands corresponding to collagen, glycosaminoglycans, lipids, and DNA. A two-dimensional analysis of the introduced opti...
Presents the results of the spectral analysis using the method of Raman scattering spectroscopy (RS) of dura mater (DM) samples, manufactured by technology “Lioplast” practised in the clinic in the area of atrophic processes at multiple gum recessions. The method of Fourier deconvolution and selection of the spectral profile by the method of least...
Изобретение относится к стоматологии и может быть использовано у пациентов, при хирургическом лечении которых выполняют костную пластику.
Способ медикаментозной поддержки пациентов при выполнении костно- пластических операций, включающий использование антибактериальных лекарственных средств в пред- и послеоперационный периоды ОТЛИЧАЮЩИЙСЯ тем, что...
An analysis of the surfaces of bone and cartilaginous biopsy specimens was performed using Raman spectroscopy. The spectral characteristics of the subchondral bone as well as the cartilaginous tissue of the loaded and unloaded areas of the articular surface were established.
Изобретение относится к медицине, в частности к хирургической стоматологии, и используется для пластики альвеолярного отростка нижней челюсти перед выполнением дентальной имплантации.
Техническим результатом предлагаемого способа является снижение трудоёмкости операции, вероятности послеоперационных осложнений; повышение остеоиндуктивных свойств ис...
There are presented the results of the analysis of the implants made of human skin by Raman scattering method. The main spectral distinctions of bioimplants by using various methods for their manufacture are shown at wavenumbers 1062 cm⁻¹, 1645 cm⁻¹, 1260 cm⁻¹, 850 cm⁻¹, 863 cm⁻¹, corresponding to components that are important for the quality of im...
Aim:
To evaluate the biocompatibility of additive materials for personified endoprostheses of hand joints in vivo.
Material and methods:
We tested a material based on titanium that was implanted into muscles and bone tissue in experiment on rabbits. Follow-up was 30 and 90 days.
Results:
Implantation into muscle tissue is accompanied by reacti...
Results of studies of testing of Raman spectroscopy (RS) method for assessment of skin implants are presented. As objects of study were used samples of rat's skin material. The main spectral differences of implants using various types of their processing appear at wavenumbers 1062 cm⁻¹, 1645 cm⁻¹, 1553 cm⁻¹, 851 cm⁻¹, 863 cm⁻¹, 814 cm⁻¹ and 1410 cm...
The results of experimental studies of healthy tooth tissue and tooth tissues during caries disease are presented. Features of Raman spectrum of tooth tissues during caries disease are obtained: the main changes are detected at wavenumbers 956 cm⁻¹.1069 cm⁻¹. corresponding to phosphates. and 1241 cm⁻¹. 1660 cm⁻¹. corresponding to collagen III and c...
The resutls of detailed analysis of donor skin implants using Raman spectroscopy method are presented. Fourier-deconvolution method was used to separate overlapping spectrum lines and to improve its informativeness. Based on the processed spectra were introduced coefficients that represent changes in relative concentration of implant components, wh...
This work presents the results of experimental studies of aortic implants using Raman spectroscopy method (RS). The features of Raman spectra of aortic implants, made by two different protocols, were obtained. Were introduced optical coefficients that allow to monitor the content of main components in aortic implants during manufacturing process an...
Raman spectra of samples of tooth enamel, dentin, and cement were obtained using an experimenta unit for noninvasive study of tooth surfaces, carious cavities, and dental pulp calcifications. Thi spectral analysis enabled us to study the distribution of mineral and organic components in soli tooth tissue, both under healthy conditions and when teet...
The effect of long-duration space flight on the vital processes in human chondroblasts in vitro was studied with a number of morphologic, biochemical and optic techniques. Object of investigation was a 3-D porous biocarrier populated by a cell culture in a leak-free vessel completely full of growth medium. The investigation was performed onboard au...
The effect of long-duration space flight on the vital processes in human chondroblasts in vitro was studied with a number of morphologic, biochemical and optic techniques. Object of investigation was a 3-D porous biocarrier populated by a cell culture in a leak-free vessel completely full of growth medium. The investigation was performed onboard au...
The results of experimental research of human cortical bone tissue depending on demineralization time were represented using Raman spectroscopy.
In this paper, we present results of the study of cell integration within cell—tissue implants under zero gravity conditions using the methods of confocal fluorescence microscopy and Raman spectroscopy (RS). The effect of factors of space flight on vital functions of cells of the human musculoskeletal system is investigated.
In this work the results of cell-tissue grafts research with a complex of optical methods – confocal fluorescent microscopy and Raman spectroscopy are presented. It was established that coefficient M scatter is related to irregularity of demineralization process. It was microscopically shown that the quantity of integrated cells into these types of...
The results of experimental studies of human teeth samples with diagnosis of chronic fibrous pulpitis and tooth decay using the method of Raman spectroscopy were presented. Based on the spectral analysis was shown that for calcifications is typical a high value of Raman band intensity at 959 cm −1 , corresponding to the stretching vibration mode ν1...
This paper presents the results of experimental studies of samples of human cortical and spongy bone tissue by means of Raman scattering as a function of the degree of demineralization. Raman spectra are obtained for samples of human cortical and spongy bone tissue as a function of the degree of demineralization. The dependence of the intensity rat...
From a position of in vivo regenerative medicine, the efficacy of plasty of osteochondral defects of the articular surface was evaluated in rabbits. The combined cell-tissue grafts were used with the demineralized spongy bone as a biomatrix and autologous or allogeneic cells from the rib cartilage tissue. Morphofunctional characteristics of the cel...
We report the results of experimental Raman spectroscopy of donor bone samples (rat, rabbit and human) with varying degrees of mineralisation. Raman spectra are obtained for the Raman bands of 950 – 962 cm-1 (PO4)3-, 1065 – 1070 cm-1 (CO3)2- and 1665 cm-1 (amide I). In demineralised bone, a sharp (98%) decrease in the intensities of 950 – 962 and 1...
The paper shows the use of confocal laser microscopy for the assessing of viability of mesenchymal bone marrow stromal cells of a rabbit with the resolution of 400 nanometers. A three-dimensional structure of cells is obtained by this method.
The research results of monitoring of viable cells in a cellular-tissue graft using confocal laser fluorescence microscopy at 488 nm and 561 nm with the use of fluorophore propidium iodide (propidium iodide, PI Sigma Aldrich USA) are presented. The processing of the received images was carried out using the software ANDOR. It is experimentally show...
The state of the surface of mesh implants and their encapsulation region in herniology is investigated by laser confocal microscopy. A correlation between the probability of developing relapses and the size and density of implant microdefects is experimentally shown. The applicability limits of differential reverse scattering for monitoring the pos...
Applicability confocal microscopy for the dynamic control of process integration of various types of cells in bone implant with wave length of 400 nanometers is shown. The quality estimation optical method of bone implant and their manufacturing technology is offered. © 2011, Institution of Russian Academy of Sciences. All rights reserved.
Clinical picture, diagnostic features and operative techniques in patients with postoperative anterolateral and lateral ventral hernias have been analyzed. Synthetic endoprosthesis were comparatively tested on human dermal fibroplast cell cultures. Taking into account and practical use of the obtained results allowed the recurrence rate decrease fr...
The new method of restoration of injured articular cartilage using plasty of intraoperative bone-cartilage defects with combined allograft based on biologic porous carrier and cell culture from stroma of costal cartilage. The transplantation of allogenic culture of chondroblast cells on demineralized spongy substance «Lioplast» ensure еру the forma...
Among 88 patients with ventral hernias of the lateral and anteriolateral localization in 32 patients tension methods were used, in 56 patients non-tension combined methods of plasty with the lay-sub lay-on lay position of the implant. In the first group local and general complications were diagnosed in 2 (6.3%), in the second in 6 (10.7%) patients....
Citations
... Cellular research methods. The study used data from [33][34][35][36][37], where dermal fibroblasts were isolated by migration from the skin fragments obtained during circulation surgery in boys under the age of 10 years according to the method of Greenberg et al. [32]. The studies using cell cultures were carried out at the Research Institute of Biotechnology "Biotech" of the Samara State Medical University. ...
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... As previous studies have indicated close associations between neuropsychiatric disorders and metabolism, including mitochondrial energy metabolism [12], glucose metabolism [13] and amino acid metabolism [14], metabolic variations detection might be a substitute for traditional methods in identification of LDD and MCI patients. Coincidentally, Raman spectroscopy (RS), as a fast, non-destructive and label-free spectroscopic technique, can provide conformational structure and specific information about metabolic variations by measuring the inelastic scattering between monochromatic photons and detected molecules [15,16]. Peripheral blood, is commonly used as the most ideal specimen for Raman acquisition, because it typically contains rich biochemical information of molecules from metabolic changes and body microenvironment as well as can be collected and measured with high convenience and repeatability. ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/1083180975165440-1635261877080_Q64/Oksana-Magsumova.jpg)
... В случае, когда процесс десневой рецессии запущен, анатомические параметры биотипа десны направляют специалиста-стоматолога к выбору правильной методики лечения [33]. ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/1049436876709888-1627216657576_Q64/Alexey_Sharov.jpg)
... В настоящее время большое применение для биомедицинских задач получили физические методы исследования [12][13][14]. Одним из методов оценки свойств поверхности имплантов с разными типами покрытий может быть метод спектроскопии комбинационного рассеяния (СКР), который является неинвазивным и в последнее время широко применяется в медицине [15]. ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/278751469424657-1443470937250_Q64/P-Timchenko.jpg)
... The apparatus of mathematical statistics, the formalism of the theory of mathematical entropy [28], the criteria of fractal geometry [29][30][31], and non-equilibrium thermodynamics of the structure of I. Prigogine [26] (criteria for the stability of a self-organizing system-the theorem on the minimum of entropy production) were used as tools for the studying of cell proliferation. In addition, morphological criteria for the cell proliferation were used [32,33]. ...
... Для исключения вклада автофлуоресценции в КР спектре использовали метод вычитания флуоресцентной составляющей полиномиальной аппроксимации с дополнительной фильтрацией случайных шумовых эффектов. Обработка и анализ спектров КР проводились с помощью программного комплекса Wolfram Mathematica 12.2 [17]. ...
... Исследования проводили in vivo с помощью метода спектроскопии комбинационного рассеяния, который был реализован с помощью стенда, включающего в себя полупроводниковый лазер (LML-785.0RB-04), оптический модуль комбинационного рассеяния (PBL 785), спектрограф (Sharmrock SR-303i) с интегрированной цифровой камерой (ANDOR DV-420A-OE), охлаждаемой до −60 • С и компьютер [16]. ...
... This could be related to the electrostatic interaction between cyromazine and negatively charged sites in CYR-CS/siRNA and CYR-CS/TPP [71]. Only CYR-CS/siRNA showed a weak peak at 1238 cm − 1 , which belonged to the characteristic absorption peak of ribonucleic acid nucleotides [72] and indicated that CYR-CS/siRNA successfully encapsulated SfCHS-siRNA (Fig. 7A). The amount of cyromazine encapsulated by CYR-CS/siRNA (74.9 mg/g) was significantly higher than that of CYR-CS/TPP (22.7 mg/g) (Fig. 7B); These two NH 3 groups on the benzene ring of cyromazine could form ionic bonds and hydrogen bonding with the phosphate group of siRNA, and the prepared siRNA nanostructures had the characteristics of adsorbing and accommodating more particles which were often used for drug delivery carriers to absorb more insoluble compounds, such as cyromazine [73]. ...
... The spectroscopy methods are the newest methods for studying biotissues. One of the promising methods is a Raman spectroscopy [7][8][9]. It is a simple to use, noninvasive, nondestructive method. ...
... It is rapidly arising as a bright device for biomedical investigations and clinical diagnosis, such as the detection of early-stage cancer, and it has been validated in countless ex vivo studies [4][5][6]. Recently, Raman spectroscopy has been applied to the investigation of bone quality in bioimplants [7]. Timchenko and his team performed a comparative analysis of the surfaces of mineralized and demineralized bone bioimplants. ...
