Klara Urbanova's research while affiliated with Czech University of Life Sciences Prague and other places

... Furthermore, it is important to disregard the potential loss of soluble nutrients, such as carbohydrates, minerals, vitamins, sugars, and proteins, which may diffuse into the blanching water [37]. In addition, the process of hot water blanching has a negative impact on the texture and microstructure of the sample [29,38]. In the end, this approach produces a substantial amount of wastewater, which gives rise to environmental problems. ...

... Through molecular docking, a large number of papers have reported that natural active molecules can be bound to PBPs [53][54][55][56]. Natural active molecules like quercetin, eugenol and kaempferol have been reported to bind effectively to PBPs [57,58]. It is worth noting that kaempferol exhibits higher specificity toward PBPs by interacting with crucial amino acids [59]. ...

... Cyperus scariosus is a perennial grass-like plant belonging to the Cyperaceae family, and is primarily found in Pakistan, India and other parts of Asia [13]. Roots, rhizomes and leaves of Cyperus scariosus have been traditionally used in Ayurvedic medicine for its various therapeutic properties, including antimicrobial, antiinflammatory, and antidiabetic activities [14]. Roots of this plant has been used as tonic, cordial, diaphoretic and vermifuge [15,16]. ...

... However, conventional hot water blanching, despite its uncomplicated equipment and straightforward operation, has certain drawbacks. The pretreatment of cassava slices with hot water blanching at a temperature of 80 ± 2 °C for 5 min yields a lower whiteness index (ranging from 80.48 to 84.05) compared to pretreatment solely through soaking [36]. On the other hand, the degradation of food quality, namely the deactivation of oxidases, can result in alterations in flavor, appearance, and the depletion of heat-sensitive components. ...

... cis-calamenene (RT = 9.52 min, VIP scores 1.14-1.16, ΔKI = 10, and total identification score of 87.9%) (Table S4 and Table S6) was found in line with previous literature on thyme phytochemical composition [32] ( Table 1). The inspection of fold change results obtained for sesquiterpenoid markers revealed the same upaccumulation trend in Polish thyme (Log2(FC) values ranging from 0.81 to 1.26) ( Table 1). ...

... Cinnamomum, Salvia, and Mentha are plant species that have been investigated for their antimicrobial properties. In vitro studies have demonstrated significant antimicrobial activity of plant extracts and EOs derived from these plants against a spectrum of foodborne pathogens, including Salmonella spp., Campylobacter spp., Listeria spp., and E. coli (Hatab et al., 2016;Huang et al., 2014;Kerekes et al., 2019;Kobus-Cisowska et al., 2019;Liang et al., 2012Liang et al., , 2019Lorenzo-Leal et al., 2019;Vihanova et al., 2021). However, factors such as extraction method, microbial strain, and chemical composition can influence their effectiveness (Burt, 2004;Gonelimali et al., 2018;Mostafa et al., 2018). ...

... aureus DSM 799, which was found to be close to the range reported for a collection of Sta. aureus strains [28]. ...

... For MIC, different concentrations of the sample were prepared using prepared solvent, and the minimum concentration zone of inhibition was recorded through plate assay followed by broth microdilution method through the guidelines of the CLSI (Clinical and Laboratory Standards Institute). Every extract sample was dissolved in the prepared solvent and diluted in nutrient broth in a range of (0.015-1) mg/ml [25,26]. We performed the antimicrobial activity against standard streptomycin (active pharmaceutical ingredients) for better results. ...

... Furthermore, the composition of these extracts, specifically the presence of phenolic compounds and their derivatives, fatty acids, terpenes, and other secondary metabolites, can also play a role in their antimicrobial activity (Altun & Yapici, 2022;Alves et al., 2022). Moreover, the volatility of plant derivatives, particularly EOs, can significantly influence the results of standard microplate-based assays, as reported by Houdkova et al. (2020). Vapor transition can affect the assay results, potentially leading to false positives in nonsealed microtiter plates. ...

... Another major component (9.5 %) of the CEO is linalool, which can notably influence or inhibit the synthesis of lipopolysaccharides, ribosomal assembly, and protein synthesis in E. coli [79]. Furthermore, the antimicrobial efficacy of α-Phellandrene, trans-Cinnamyl acetate, and β-Caryophyllene was reported in the literature [80][81][82]. These three elements accounted for 21.72 % of the CEO prepared in current research. ...