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June 1986
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234 Reads
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46 Citations
Environmental Entomology
The ability of honey bees, Apis mellifera L., to transfer pollen within the hive through contact with nestmates was tested. In almost all cases, bees acquired pollen from nestmate contacts. Pollen viability was tested by pollinating self-incompatible apple blossoms with treated bees. The contribution of in-hive pollen transfer to cross-pollination and fruit set in apple is discussed.
January 1986
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9 Reads
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3 Citations
Journal of Electron Microscopy Technique
Glutaraldehyde-fixed insects were embedded in Tissue-TekR and sectioned on a liquid-nitrogen-cooled stage. The sections were sandwiched between two layers of microscope lens paper and all postsectioning treatments were carried out on this sandwich, including dehydration and critical-point drying. In some cases, the sections were placed on filter membranes and lyophilized. These procedures produced intact specimens which maintained internal morphology as well as inter- and intracellular integrity without expensive or specialized equipment.
... Moreover, the findings of the present study are in agreement with those of previous GBS-based studies 80 . However, the low diversity observed in most Korean Malus populations is unusual for Malus (Table 1), which is self-incompatible and relies on cross pollination 81 . Other evolutionary forces affecting genetic diversity include mode of reproduction, geographical distribution, and population size, among which the mode of reproduction is highly relevant as it involves direct genetic exchange 82 . ...
June 1986
Environmental Entomology
... The sample should, therefore, be correctly oriented to image a desired morphological feature. The only way to create 3D models is through cryosections, leading to a tedious sample preparation [4]. Moreover, insect specimens with hairs, spines, and other projections are particularly prone to charging, even at low accelerating voltages, producing charging lines of streaks at the final image. ...
January 1986
Journal of Electron Microscopy Technique
