K L Brown's research while affiliated with University of Washington Seattle and other places
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Publications (5)
Two genes encoding the predominant polypeptides of Bacillus thuringiensis subsp. thompsoni cuboidal crystals were cloned in Escherichia coli and sequenced. The polypeptides have electrophoretic mobilities of 40 and 34 kDa, with the deduced amino acid sequences predicting molecular masses of 35,384 and 37,505 Da, respectively. No statistically signi...
Two sigma factors, sigma 35 and sigma 28, direct transcription from the Bt I and Bt II promoters of the cryIA(a) gene of Bacillus thuringiensis; this gene encodes a lepidopteran-specific crystal protoxin. These sigma factors were biochemically characterized in previous work (K. L. Brown and H. R. Whiteley, Proc. Natl. Acad. Sci. USA 85:4166-4170, 1...
A crystal protein gene of Bacillus thuringiensis subsp. kurstaki HD-1-Dipel is transcribed in vivo from two overlapping promoters that are activated at different times during sporulation. We reported earlier (K. L. Brown and H. R. Whiteley, Proc. Natl. Acad. Sci. USA 85:4166-4170, 1988) that an RNA polymerase containing a sigma subunit with an appa...
We report the isolation of an RNA polymerase from sporulating cells of Bacillus thuringiensis subsp. kurstaki HD-1-Dipel that directs transcription from the promoter region of an insecticidal crystal protein gene. The core components of this RNA polymerase are associated with a polypeptide that has an apparent mass of 35 kDa. Neither RNA polymerase...
Citations
... The data on Bt I and Bt II promoter utilization in B. subtilis are confusing, because the level of # in B. subtilis, as detected by reaction with a monoclonal antibody, was reported to decline after t (Trempy et al. 1985). Whiteley et al. (1990) suggested that some other regulatory mechanisms could account for the continued expression from the Bt I promoter in B. subtilis. Therefore we have quantified the contributions of Bt I and Bt II promoters to cryIAa expression, using SigE\ and SigK\ mutants of Bt. ...
... For detecting presence of protein crystals and screening of original Bt srains and determine the shapes & size of spores and crystals, phase contrast microscopy is used (Benhard, 1986;Brown and Whiteley, 1990;Chilcott and Wigley, 1988;DeLucca et al., 1982;Hongyu et al., 2000 ;Padua et al., 1984). Based on phase contrast microscopy Bt strains were divided into bipyramidal (53.0%), irregularly pointed (24.0%), spherical (9.0%), irregularly spherical (8.0%) and rectangular (6.0%). ...
Reference: Rajashekhar -20414 (M.Sc thesis)
... It is noteworthy that the insecticidal activity of the components of different binary toxins can be varied from one insect species to another. In the Cry15Aa/40-kDa complex protein, the 40-kDa protein neither was toxic nor showed synergistic activity for the toxicity of Cry15Aa against the tested lepidopteran insect species, while the second partner, Cry15Aa toxin, has been found toxic (Brown and Whiteley, 1992;Naimov et al., 2008). While, in the case of Cydia pomonella, there are contradictory results that claim both the synergistic activity of the 40-kDa protein (Rang et al., 2000) and also mention the lack of contribution of 40-kDa protein in the toxicity of Cry15Aa (Rang et al., 2000;Naimov et al., 2011). ...
... Encapsulation Cry1Ba in the mother cell of sigK mutant also increasing UV radiation resistance (Zhou et al., 2014). However, some cry genes are regulated by σ K , and cry gene expressions were reduced in the sigK deletion mutant (Adams et al., 1991;Bravo et al., 1996). ...
... Transcription from these promoters is initiated by σ E and σ k like factors [92]. For instance, the cry1 Aa gene is transcribed during the sporulation phase from two sporulation-specific promoters [93,94,95]. In B. thuringiensis subsp. ...
