Jing Chen's research while affiliated with Chinese Academy of Agricultural Sciences and other places
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Publications (24)
The Hepatitis E virus (HEV) causes acute and chronic Hepatitis E and is a global public health concern. HEV genotypes 3 (HEV-3) and 4 (HEV-4) are common to humans and animals, and domestic pigs and wild boars have been identified as the main reservoirs. However, limited information is available on the status of HEV infection in pigs, particularly i...
Introduction
Porcine deltacoronavirus (PDCoV) is a zoonotic pathogen with a global distribution, capable of infecting both pigs and humans. To mitigate the risk of cross-species transmission and potential outbreaks, it is crucial to characterize novel antiviral genes, particularly those from human hosts.
Methods
This research used HIEC-6 to invest...
Porcine circoviruses disease (PCVD), caused by porcine circovirus (PCVs), is an important swine disease characterized by porcine dermatitis, nephrotic syndrome and reproductive disorders in sows. However, diseases caused by PCV2, PCV3, or PCV4 are difficult to distinguish, so a simple, rapid, accurate and high-throughput diagnostic and identificati...
Porcine Deltacoronavirus (PDCoV) is a newly identified coronavirus that causes severe intestinal lesions in piglets. However, the understanding of how PDCoV interacts with human hosts is limited. In this study, we aimed to investigate the interactions between PDCoV and human intestinal cells (HIEC-6) by analyzing the transcriptome at different time...
Rotavirus (RV) is one of the most significant pathogens in humans and animals with diarrhea worldwide. Cell entry is the first step in viral infection, and the outer capsid protein VP4 is crucial for RV attachment and internalization. In order to discover novel candidate host factors involved in RV cell entry, a proximity labeling method was applie...
Circoviruses are a group of small circular, single-stranded DNA viruses that belong to the family Circoviridae. They are known to infect a wide variety of animals. Rhizomys sinensis is a species of rodent that is the reservoir of many zoonotic pathogens. Our previous study identified many sequencing reads mapped to the genome of viruses in Circovir...
Introduction
Pseudorabies (PR) is a highly contagious viral disease caused by the pseudorabies virus (PRV), which can cause disease in a wide range of domestic and wild animals. Studies have shown that new mutant strains have emerged in pig farms in many regions and that commercial inactivated and live attenuated vaccines are becoming less effectiv...
Type III interferons (IFNLs) have critical roles in the host's innate immune system, also serving as the first line against pathogenic infections of mucosal surfaces. In mammals, several IFNLs have been reported; however, only limited data on the repertoire of IFNLs in avian species is available. Previous studies showed only one member in chicken (...
COVID-19 has globally spread to burden the medical system. Even with a massive vaccination, a mucosal vaccine offering more comprehensive and convenient protection is imminent. Here, a micro-sized vaccine based on recombinant Lactiplantibacillus plantarum (rLP) displaying spike or receptor-binding domain (RBD) was characterized as microparticles, a...
The pandemic of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has posed great threat to the world in many aspects. There is an urgent requirement for an effective preventive vaccine. The receptor binding domain (RBD), located on the spike (S) gene, is responsible for binding to the angiotensin-converting enzyme 2 (ACE2) rec...
Nipah virus (NiV) is a newly emerged extremely dangerous zoonotic pathogen highly fatal to humans. Currently, no approved vaccine is available against NiV. This study employed a mammalian eukaryotic system to express NiV soluble G glycoprotein (NiV-sG), using CpG oligodeoxynucleotides (CpG)/Aluminum salt (Alum) as adjuvants to obtain a recombinant...
Type III and type I interferon have similar mechanisms of action, and their different receptors lead to different distributions in tissue. On mucosal surfaces, type III interferon exhibits strong antiviral activity. Porcine epidemic diarrhea virus (PEDV) is an economically important enteropathogenic coronavirus, which can cause a high incidence rat...
SARS-CoV-2, the causative agent of the COVID-19 pandemic, undergoes continuous evolution, highlighting an urgent need for development of novel antiviral therapies. Here we show a quantitative mass spectrometry-based succinylproteomics analysis of SARS-CoV-2 infection in Caco-2 cells, revealing dramatic reshape of succinylation on host and viral pro...
Coronavirus Disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2), has posed a constant threat to human beings and the world economy for more than two years. Vaccination is the first choice to control and prevent the pandemic. However, an effective SARS-CoV-2 vaccine against the virus infection is stil...
Coronavirus infections cause diseases that range from mild to severe in mammals and birds. In this study, we detected coronavirus infections in 748 farmed wild animals of 23 species in Guangdong, southern China, by RT–PCR and metagenomic analysis. We identified four coronaviruses in these wild animals and analysed their evolutionary origins. Corona...
Porcine epidemic diarrhea (PED) and transmissible gastroenteritis (TGE) caused by porcine epidemic diarrhea virus (PEDV) and transmissible gastroenteritis virus (TGEV) are two highly contagious intestinal diseases in the swine industry worldwide. Notably, coinfection of TGEV and PEDV is common in piglets with diarrhea-related diseases. In this stud...
[This corrects the article DOI: 10.1371/journal.ppat.1008421.].
The coronavirus disease 2019 (COVID-19) outbreak has significantly affected international public health safety. It has been reported that the pathogen severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes COVID-19, could originate from bats and utilize the Malayan pangolin (Manis javanica) as an intermediate host. To gain furth...
The outbreak of a novel corona Virus Disease 2019 (COVID-19) in the city of Wuhan, China has resulted in more than 1.7 million laboratory confirmed cases all over the world. Recent studies showed that SARS-CoV-2 was likely originated from bats, but its intermediate hosts are still largely unknown. In this study, we assembled the complete genome of...
The outbreak of 2019-nCoV pneumonia (COVID-19) in the city of Wuhan, China has resulted in more than 60,000 laboratory confirmed cases, and recent studies showed that 2019-nCoV (SARS-CoV-2) could be of bat origin but involve other potential intermediate hosts. In this study, we assembled the genomes of coronaviruses identified in sick pangolins. Th...
Influenza viruses often pose a serious threat to animals and human health. In an attempt to explore the potential of herbal medicine as a treatment for influenza virus infection, eleutheroside B1, a coumarin compound extracted from herba sarcandrae, was identified, which exhibited antiviral and anti‑inflammatory activities against influenza A virus...
We report here the complete genome sequence of the parainfluenza virus PIV5-GD18 strain, isolated from a wild Sunda pangolin (Manis javanica) in China in 2017. It was 15,246 nucleotides with four nucleotides substitutions, which resulted in four changes of amino acid that were found only in PIV5-GD18, which further broadens the PIV5 infection host...
Interferon-inducible transmembrane protein 3 (IFITM3) inhibits the replication of multiple pathogenic viruses by blocking their entry. In this study, we constructed a shuttle plasmid, harboring human IFITM3. Thereafter, recombinant adenovirus rAd5-IFITM3 was obtained by co-transfection of the linearized viral backbone vector pAd5 and the shuttle pl...
Influenza A viruses of subtype H1N1 have been reported widely in pigs in China, associated with clinical disease. These mainly include classical swine H1N1, avian-like H1N1, and human-like H1N1 viruses. In this study, we reported a novel triple-reassortant H1N1 virus (A/swine/Guangdong/1/2010) containing genes from the classical swine (NP, NS), hum...
Citations
... In PL, the target protein is fused with TurboID, a biotinylated enzyme that can catalyze endogenous proteins in a proximitydependent manner, which can quickly and non-toxically label the protein interaction with the target protein or its proximal protein in just 10 min in the biotin-containing environment [12]. The high efficiency of TurboID has led to its application in the study of a variety of animal viruses, including SARS-CoV-2, hepatitis B virus (HBV), herpes virus, and rotavirus [7,[13][14][15]. ...
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... one of the major domestic animals that have an important role in food security and socio-economic starting point (Das et al., 2022). AD caused by PRV is a common disease in many animals, especially in pigs (Zhao et al., 2023). AD is a devastating pig disease and an important viral disease in pigs all over the world, which is characterized by high incidence rate in piglets and reproductive problems in sows and boars (Pegu et al., 2016;Delva et al., 2022). ...
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... It indicates that MRPS6 can activate various downstream components from IFN-b pathway. Considering that IRF-3 is the primary transcription factor for IFN-b [38], we investigated the impact of MRPS6 on IRF-3 in HIEC-6 cell. Overexpression of MRPS6 in HIEC-6 cells resulted in increased protein and mRNA levels of IRF-3 ( Figures 7G, H). ...
... This observation is supported by the fact that the total amount of antigen that was used for immunization was very low, 1.4 to 1.6 mg for RBD 318-543 , 0.8 mg for RBD 462-510 , and 0.35 mg for cCD20. Conventional doses for immunization contain between 20 and 100 mg of antigen mixed with adjuvant [32,[48][49][50]. Notably, the best antibody yield was achieved when the BALB/c mice were immunized with the construct displaying the cCD20 loop on the surface. ...
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... Currently available vaccines stimulate the production of neutralizing antibodies (Abs) against viral surface proteins, particularly the receptor-binding domain (RBD), thereby preventing the virus from the receptor binding required for viral entry and in turn infection. Accordingly, spike (S) protein is a common target for coronavirus vaccine research, and SARS-CoV-2 S protein is also the target of available COVID-19 vaccines [2][3][4]. However, variants of SARS-CoV-2 have mutations in S proteins, including in the RBD region, which can reduce the efficacy of these vaccines [5]. ...
... For example, SARS--CoV-2 infected individuals may have higher blood glucose and fatty acid concentrations and abnormalities in amino acid metabolism [17]. In SARS-CoV-2-infected cells, viral nonstructural protein 14 (NSP14) promotes the succinylation of several key enzymes in the TCA cycle that interact with SIRT5, thereby inhibiting cellular metabolic pathways [18]. Rhinovirus infection rapidly enhances the expression of enzymes responsible for glucose utilization and uptake, leading to a highly anabolic state [19]. ...
... The raw sequencing reads were processed to obtain clean data using Soapnuke (v2.0.5) for further analysis [19]. Quality-filtered reads were then de novo assembled to generate the metagenome. ...
... We have determined the virus titer as previous description (34). Briefly, HIEC-6 or Caco2 cells were seeded in 96-well plates at a density of 1×10 5 cells per well. ...
... Datasets supporting assembly of GD PCoV MP789 are also problematic. Two datasets, an amplicon dataset and dataset GZ1-2 were provided by Liu et al. (2021) in support of MP789 genome generation in addition to two samples from Liu et al. (2019). Dataset GZ1-2 had a very low Pangolin CoV MP789 read count, with only 16 reads with unique coverage, including 6 SNVs and a 6nt insert relative to MP789. ...
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... Pangolins have recently gained attention as a potential host of emerging viral diseases after coronaviruses related to SARS-CoV-2 were reported in the Malayan pangolin [5][6][7][8][9][10]. Pangolins have also been recognized as hosts for other RNA viruses such as canine distemper virus (Paramyxoviridae), which is associated with respiratory, digestive and neurological illness in pangolins [11], flaviviruses, reoviruses, pneumoviruses and picornaviruses [12][13][14][15]. ...
