Jiamin Qiu's research while affiliated with Purdue University and other places

... We believe that this difference might be associated with the animal strain and the species used for HFD administration. Balancing energy intake and expenditure is vital for maintaining body weight (37,38). To determine the role of glutamine in weight gain, we measured food intake and energy metabolic parameters using a metabolism cage. ...

... In eukaryotic cells, there is a highly dynamic and organized interaction between organelles and the cytoskeleton to coordinate intricate biological processes. Organelle crosstalk involves the communication and coordination between different organelles within a cell to regulate their function and maintain cellular homeostasis and has been an area of intensive investigation [32][33][34]. As mentioned earlier, organelle crosstalk is achieved by the mutual contact of organelle membranes, which are specialized biological membranes that surround and compartmentalize various organelles within eukaryotic cells. ...

... Indeed, our recent study has demonstrated that myofiber-specific restoration of LDs rescues the phenotype of myofiber-specific Prmt5-KO mice very effectively. 82 Future studies should explore the possibility of targeted delivery of HCQ into muscle cells using for example nanoparticle delivery tools. 83,84 STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: ...

... Recent developments of nano-DESI MSI instrumentation have enabled imaging with high spatial resolution and high throughput. [45][46][47][48] Furthermore, we have developed approaches for improving the extraction and ionization efficiency of analytes by tailoring the composition of the extraction solvent. [49,50] These efforts have enhanced the sensitivity and depth of molecular coverage of the technique. ...

... Numerous neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS) and Parkinson's disease, have been associated with mutations in the CHCHD2 gene [19,20]. Moreover, CHCHD10 is involved in regulating mitochondrial structure and function [21]. ...

... LDs also play a significant role in lipid metabolism, participating in the synthesis and degradation of various lipid species. Furthermore, LDs interact with other organelles, including mitochondria, peroxisomes, and lysosomes, facilitating lipid exchange and signaling events [29]. The dynamics of LDs are influenced by a variety of factors. ...

... Ebf2 deficiency causes BAT developmental abnormalities although this is eventually compensated for in adult mice [128]. Recently, loss of the mitochondrial protein Letm1 domain containing 1 (Letmd1) was shown to produce severe abnormalities in BAT mitochondria and the expression of thermogenesis related genes, including UCP1, and caused the knockout mice to die from hypothermia upon cold exposure, resembling the UCP1 null mice [129,130]. β3-Adrenoreceptor-stimulated increases in energy expenditure are completely abolished in these mice. Consistent with the idea that Letmd1 modulates UCP1 abundance, the physiological expression of Letmd1 correlates highly with the UCP1 expression and BAT thermogenic activity. ...

... [15][16][17] In addition, isomer-selective imaging of unsaturated lipids has been achieved by combining chemical derivatization with tandem mass spectrometry (MS) of the products. [18][19][20][21][22] Although these developments substantially enhance the analytical performance of MSI, they often increase the total acquisition time by either sampling more positions to increase the spatial resolution, or increasing the perpixel acquisition time for obtaining a broader molecular coverage. ...

... Some of these challenges have been addressed using tandem mass spectrometry (MS/MS) imaging experiments, which enable simultaneous imaging and identification of molecules in biological samples [24,25]. In addition, derivatization approaches including online singlet oxygen reaction [26] and on-tissue Patern oÀBüchi [27] or ozonolysis reactions [28] have been coupled with MSI for studying the localization of positional isomers of phospholipids. However, these experiments are typically limited to a targeted list of m/z windows. ...

... Recently, ambient ionization MS coupled with novel ion activation technologies, such as Paternò-Büchi reaction [77], ozone-induced dissociation [78], epoxidation [79], light-controlled photoepoxidation [80], electrochemical epoxidation [81], chloroauric acid-dopped solvent-induced epoxidation [82], low-temperature plasma-induced epoxidation [83], and selective photosensitized oxidation induced by singlet oxygen [84], have yielded more informative and abundant specific fragment ions allowing rapid and direct structural elucidation of double bonds, cis/trans isomers, and sn-position isomers of GSLs and other lipids [85]. The principles of these methods are well described in other reviews [86,87]. ...