J. R. Coulter's research while affiliated with University of California and other places

... Red fluorescence represents relative photopigment abundance (Lesser, 1996;Lee et al., 2012;Cooper et al., 2014). Side scatter is a representation of cell shape or roughness, and forward scatter is a representation of cell size or cell volume (Mullaney et al., 1969;Steen, 1980;Shapiro, 2003;Tzur et al., 2011). The full protocol with details on sample preparation, data curation, and analysis is publicly available on protocols.io ...

... Immunological naming strategies have additionally been applied to the division of explicit kinds of cells. Cells named with fluorescent antibodies or fluorescent immune microspheres have been isolated from unlabeled cells by fluorescence-initiated cell arranging [40,41]. Cells explicitly named with these reagents have been isolated from unlabeled cells based on their maintenance in attractive fields [42]. ...

... In the history of cytometry technology, the development of flow cytometry was a watershed moment, thousands of cells to be measured in seconds. [38][39][40][41][42][43] As early as the 1960s, a group of Review researchers in the United States developed the first automated cell counting systems using two different physical principles: the first based on impedance measurement (Coulter's principle), and the second devised by Herzenberg based on optical analysis (light scatter). 26 The first family of instruments evolved into the so-called cell counters, which are still widely used in all analytical laboratories for automated counting, mainly of the various cell subpopulations in the blood (or other body fluid). ...