Isabelle Gaillard's research while affiliated with French Institute of Health and Medical Research and other places

... Further, no differences in Angpt2 expression among the three groups were observed (Fig. 1). These results corroborated those of previous reports [11][12][13] . Even the 2-h ACTH stimulation of the adrenal gland in the present study suggested that ACTH regulates angiogenic factors which could affect adrenal autograft conditions. ...

... Furthermore, activation of PKA has been shown to either enhance or impede cell adhesion [25,45,50,[52][53][54][55][56][57][58] , in a cell-and/or ECM-specific manner. Finally, FAs contain a number of bona fide PKA substrates [25] , including Ena/VASP proteins [59,60] , the LIM & SH3 domain protein (LASP-1 [61] ), Src-family kinases (Src [62,63] and Fyn [57] ), SHP2/PTPN11 [64,65] , and integrin α4 [29] . To this end, we set out to determine if PKA is a functional constituent of FAs. ...

... ACTH increases cytoplasmic cAMP production and hence protein kinase A activity, a process regulated by its regulatory subunits (PRKAR1A, PRKAR1B, PRKAR2A, PRKAR2B) and phosphodiesterases in steroidogenesis. ACTH stimulates the growth of the adrenal cortex through cellular proliferation in stress and disease, as well as the growth of vascular endothelial cells in the adrenal cortex (66)(67)(68). Angiotensin II sustains and regulates the zona glomerulosa, stimulating aldosterone production by zona glomerulosa cells as part of the renin-angiotensin-aldosterone system (50,69). The extracellular matrix is also thought to modulate the cellular response to ACTH, angiotensin II, and growth factors (50). ...

... As stated above, upstream events involve a rapid and transient increase in total PTP activity after hormone stimulation and the induction of tyrosine dephosphorylation of endogenous proteins. Indeed, the use of compounds that inhibit PTP enzymatic activity results in a decrease in cAMP-stimulated ACSL4 protein levels (Cano et al. 2006), cAMP-stimulated tyrosine dephosphorylation of paxillin, and focal adhesion disassembly (Vilgrain et al. 1998). Accordingly, the inhibition of PTP activity affects both StAR protein and mRNA synthesis, which suggests that the activity of hormone-regulated PTPs is a requirement in the LH and ACTH signaling cascade. ...

... Interestingly, in several tumor types 3D models have been introduced into research modalities however they are lacking regarding endocrine tumor types. To date, four of each adrenocortical carcinoma, pituitary neuroendocrine tumor (pitNET) and pheochromocytoma [9][10][11][12][13][14][15][16][17][18][19][20] reports have been published using 3D cultures, they are different in terms of the applied methods and no viability and lifespan analysis have been tested and compared among different culture conditions. Therefore, in the use of 3D culturing in routine research applications the methodology should be optimized and standardized in order to maintain viability observed in vivo systems. ...

... The rounding-up of the cells following ACTH stimulation is correlated with both a loss of focal adhesions 428 and a specific decrease in paxillin phosphorylation. This latter effect is mediated by the phosphotyrosine 429 phosphatase, SHP2 (Rocchi, et al. 2000), itself activated by PKA-dependent serine phosphorylation. This 430 last step has been reported to be essential for cAMP-induced corticosterone secretion (Cooke et al. 2011; 431 Gallo-Payet & Battista 2014; Sewer & Li 2008). ...

... Interestingly, in several tumor types 3D models have been introduced into research modalities however they are lacking regarding endocrine tumor types. To date, four of each adrenocortical carcinoma, pituitary neuroendocrine tumor (pitNET) and pheochromocytoma [9][10][11][12][13][14][15][16][17][18][19][20] reports have been published using 3D cultures, they are different in terms of the applied methods and no viability and lifespan analysis have been tested and compared among different culture conditions. Therefore, in the use of 3D culturing in routine research applications the methodology should be optimized and standardized in order to maintain viability observed in vivo systems. ...