Irvin A. Lampert's research while affiliated with West Middlesex University Hospital and other places

... Since 2003, the Human Protein Atlas (HPA) project has systematically explored the human proteome in a range of normal tissues, cancers and cell lines. The effort is based on a unique set-up of high throughput generation of affinity-purified polyclonal antibodies for in situ protein detection using immunohistochemistry (IHC) and immunofluorescence (IF) on carefully designed tissue-and cell microarrays [1][2][3]. The output of the project is a publically available Protein Atlas [4], in which all IHC and IF images along with antibody validation and annotation data are published. ...

... 20 Conversely, the other half of DLBCL patient-associated mutations disrupt DNA binding, causing loss of function. 55 Furthermore, FOXO1 gain-of-function mutations occur at a higher rate in follicular lymphoma 56 and Burkitt lymphoma, 57,58 which could indicate that disease etiology plays a role in the positive selection of FOXO1 mutant clones. Thus, tumors derived from cells at different stages of the GC reaction may be differentially dependent on FOXO1. ...

... Bone marrow (BM) fibrosis develops in association with various conditions in addition to hematologic-neoplasms such as primary myelofibrosis (PMF), including autoimmune disease, vitamin D deficiency, and human immunodeficiency virus infection (1). Marrow fibrosis is thought to result from the activity of cytokines produced by the abnormal megakaryocytes associated with hematologic-neoplasms (2). ...

... The blood smear provides interesting although non-specific elements: anisopoikilocytosis with the presence of numerous macrocytes, dacryocytes and, in the case of vitamin B12 deficiency, schistocytes. Hypersegmented neutrophils are also observed [3]. ...

... 15 The bone marrow biopsy can show varying degrees of paratrabecular and intertrabecular fibrosis with a moderate increase in vascularity of the marrow. Macroscopic cysts and hemosiderin-laden macrophages can also be seen, and this final stage is referred to as osteitis fibrosa cystica 16 ; however, this is very rare nowadays in view of earlier diagnosis and treatment. These features are important to remember as bone marrow biopsy is occasionally performed to investigate either hypercalcemia or radiographic lesions suspicious of metastatic carcinoma in patients with unsuspected severe hyperparathyroidism. [17][18][19] There are no specific peripheral blood or bone marrow aspirate abnormalities associated with PHPT, although mild anemia may occur. ...

... As it is known, phagocytosis represents an immune process which involves attachment and binding of Fc antibody fragments and C3b complement fractions to particular receptors described on leukocyte membranes, engulfment and subsequent intracellular fusion of lysosomes with phagocytic vacuoles, followed by cellular biochemical digestion or non-self molecular elements (98) . Hemophagocytosis should be considered an overactivated form of phagocytosis, in which the increased activity of histiocytes may be described in infections, various inflammatory diseases (such as autoimmune and autoinflammatory) and even in malignancies (99) . ...

... A proportion of normal erythroblasts have few (1)(2)(3)(4)(5) iron-containing granules randomly distributed in the cytoplasm. Such erythroblasts are designated as sideroblasts. ...

... 12 Previous IHC use in prostate cancer and melanomas have yielded excellent results that are largely consistent with the location of MYC. 18,19 Recently, antibodies targeting the MYC N-terminus have been produced: N-262 (rabbit polyclonal) and Y69 (rabbit monoclonal). Both gave excellent results largely consistent with molecular studies regarding the localisation of MYC in normal and tumour tissue. ...

... rates (FNA) and reported that chromosome 7 & 13 copy number had the same adverse prognosis as has been shown in sporadic BL. This team also provides remote review of FNA by video-linkage to help confirm morphological diagnoses in the setting of limited immunohistochemistry (Zhou et al, 2015). The French collaboration with North Africa that has been in place for many years (Franco-African Paediatric Oncology Group [GFAOP]) is now able to demonstrate greatly improved outcomes. ...

... [11] Colourless, refractile oxalate crystal deposition in the bone marrow interstitium occurs in oxalosis. [12] Macrophages laden with cholesterol and cystine crystals may be identified in hyperlipidaemic states and in cystinosis respectively. [12] All of these appear different to the eosinophilic crystals described in the current case. ...