January 2021
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Millet and rice are two very essential bowls of cereal in the Senegalese daily food. Their storage gets a real problem because of the insects that depreciate those causing huge losses in terms of quantity and quality. The Lepidoptera C. cephalonica is one of these pests. The objective of this study is to characterize genetically the populations of C. Cephalonica on the millet and rice stocks in the Center of the Groundnut Basin and the Senegal River Delta thanks to the Cytochrome b gene to see if the environment and the food substrate affect the genetic structuration of these populations, (biotype and ecotype aspects). We have sequenced a portion of cytochrome b by the Next Generation Sequencing (NGS) method after extraction and amplification by Polymerase Chain Reaction (PCR). The genetic analyses (variability and genetic diversity indices, the populations' genetic structure, the demographic evolution, and the phylogenetic approach) have revealed a high genetic diversity in the global population with the presence of seventy-three (73) haplotypes for ninety-one (91) sequences. The populations of C. cephalonica might issue from a stable ancestral population (haplotypic diversities and strong nucleotide and non-significant Tajima D). The Molecular Variance Analysis (AMOVA) inferences and phylogenetic trees have shown that there is a significant genetic differentiation depending on the environment and the food support between the moth populations.