Huayi Pan's research while affiliated with Nanjing Tech University and other places

... Chiral amines are vital building blocks and have been widely applied in pharmaceutical and fine chemical industries (Wang et al. 2023). Of late, biosynthetic methods have attracted growing attention for manufacturing chiral amines via biocatalysts instead of chemocatalytic methods, such as transaminases, amine oxidases, and amine dehydrogenases (Tang et al. 2023). Transaminases are pyridoxal-5 0 -phosphate (PLP)dependent enzymes that can catalyze the transfer of an amine group between a donor amine molecule and a carbonyl acceptor, leading to asymmetric synthesis of chiral amines (Nugent and El-Shazly 2010;Ghislieri and Turner 2014;Schrittwieser et al. 2015;Slabu et al. 2017;Yin et al. 2020). ...

... UGT76G1 is a key enzyme in the production of Rebaudioside. Research has shown that a single-point saturation mutation in the UGT76G1 gene converts Rebaudioside M to Rebaudioside E, which improves its performance and sweetening properties by 2 times [12]. ...

... Modifications of steviol glycosides were also described using metabolically engineered yeast and bacteria in whole-cell biotransformation [12−16] . However, the majority of reported research on changing the glycosylation of steviol glycosides uses enzyme preparations was comprehensively reviewed [17] and can be divided into the use of recombinant glucosyltransferases [18−22] , fructofuranosidases [23−26] , cyclodextrin glucanotransferases [27−31] , glucosidases [32−36] , and galactosidases [37−39] . Interestingly, no attempts to perform biotransformation of steviol glycosides using glycosynthases have been reported yet. ...