Hua Li's research works | Xi'an Shiyou University and other places

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Fig. 1 Characterisation of liposomes incorporating VP and gold nanoparticles. a Photograph of liposome samples and pure gold colloidal solution. b Absorption spectra of different liposome samples and pure gold colloidal solution. Red arrows indicated typical absorption peaks of VP (~ 410 nm and ~ 689 nm), 5 nm gold nanoparticles (~ 515 nm and 10 nm gold nanoparticles ~ 517 nm). c-e TEM images of 10 nm gold nanoparticles (c) pure liposomes (d) and liposomes loaded with 10 nm gold nanoparticles (e) Red arrows indicate gold nanoparticles encapsulated inside the liposomes

Fig. 2 1 O 2 generation from different liposome samples under X-ray radiation. a Relative SOSG fluorescence intensities from liposome samples at different X-ray doses. b Percentage increase in SOSG fluorescence intensities from different liposome samples under 4 Gy X-ray radiation. Error bars show standard deviation from four measurements

Fig. 3 Intracellular 1 O 2 production from liposomes with and without X-ray radiation. (a-h) Representative confocal laser scanning microscopy images of SOSG in a HCT116 cells without any treatment, b HCT116 cells after 4 Gy X-ray irradiation, c HCT116 cells treated with Lipo-VP (250 µM) for 4 h, d HCT116 cells treated with Lipo-VP (250 µM) for 4 h and 4 Gy X-ray irradiation, e HCT116 cells treated with TPP-Lipo-VP (250 µM) for 4 h, f HCT116 cells treated with TPP-Lipo-VP (250 µM) for 4 h and 4 Gy X-ray irradiation, g HCT116 cells treated with Lipo-VP-10Au (250 µM) for 4 h and h HCT116 cells treated with Lipo-VP-10Au (250 µM) for 4 h and 4 Gy X-ray irradiation. Scale bar is 70 µm. g Quantitative analysis of 1 O 2 generation in HCT116 cells at different treatment conditions

Fig. 4 Colocalization of Lipo-VP and TPP-Lipo-VP with the mitochondria and lysosomes. a-c Representative confocal laser scanning microscopy images of VP (pseudo green colour to distinguish between VP and trackers), Mitotracker Red (red channel) and LysoTracker (red channel) in HCT116 cells after incubation with Lipo-VP (250 µM) and TPP-Lipo-VP (250 µM). Merged images of green and red channels indicated colocalization level of the liposomes and mitochondria (or lysosomes). Scale bar is 20 µm

Fig. 7 Apoptosis/necrosis assays performed in HCT116 cells at 24 h after the treatments. a-d Representative confocal laser scanning microscopy images of the cells after different treatments as indicated. The concentration of TPP-Lipo-VP-10Au used to treat the cells was 250 µM. Scale bar is 70 µm. e Relative changes in levels of apoptosis and necrosis upon different treatments as indicated in a-d. f Relative protein expression was analysed by using ImageJ software. Protein expression was normalized by use of the loading control protein (β-actin)

X-ray induced photodynamic therapy (PDT) with a mitochondria-targeted liposome delivery system

June 2020

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38 Citations

Journal of Nanobiotechnology

[...]

In this study, we constructed multifunctional liposomes with preferentially mitochondria-targeted feature and gold nanoparticles-assisted synergistic photodynamic therapy. We systemically investigated the in vitro X-ray triggered PDT effect of these liposomes on HCT 116 cells including the levels of singlet oxygen, mitochondrial membrane potential, cell apoptosis/necrosis and the expression of apoptosis-related proteins. The results corroborated that synchronous action of PDT and X-ray radiation enhance the generation of cytotoxic reactive oxygen species produced from the engineered liposomes, causing mitochondrial dysfunction and increasing the levels of apoptosis.

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... Inflicting damage to mitochondria is a well-known target of ROSs generated via PDT [59]. Some researchers even focus solely on mitochondria-targeted PDT [62]. ...
Reference:
Lipid-Coated Polymeric Nanoparticles for the Photodynamic Therapy of Head and Neck Squamous Cell Carcinomas

X-ray induced photodynamic therapy (PDT) with a mitochondria-targeted liposome delivery system

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June 2020

Journal of Nanobiotechnology

[...]

Hua Li's research while affiliated with Xi'an Shiyou University and other places

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