Hua Guo's research while affiliated with Tianjin University of Traditional Chinese Medicine and other places
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Publications (6)
Background
Berberidis Radix (Sankezhen), a typical multi-origin Chinese medicinal material, originates from the dried roots of the Berberis genus plants. Previous studies have shown that there were significant differences in chemical compositions in different Berberis species. Therefore, it was exceedingly important to accurately identify different...
Mantidis Ootheca (Sangpiaoxiao, mantis egg case) is a typical multi-origin Chinese medicinal material. The Chinese Pharmacopoeia stipulates that the Mantidis Ootheca originates from three species of Mantis: Tenodera sinensis, Statilia maculate, and Hierodula patellifera. However, Mantidis Ootheca mainly relies on field collection, which leads to co...
Mantidis Ootheca (Sangpiaoxiao) is a typical multi-origin Chinese medicinal material. The Chinese Pharmacopoeia stipulates that the Mantidis Ootheca originates from Tenodera sinensis , Statilia maculate , and Hierodula patellifera . However, Mantidis Ootheca mainly relies on field collection, which leads to confusion of its actual origin in the mar...
Radix Paeoniae Rubra (Chishao), a typical multi-origin Chinese medicinal material, originates from the dried roots of Paeonia lactiflora or P. veitchii. The previous study suggested that these two commonly used Chishao showed variation in their chemical compositions and clinical efficacies. Therefore, accurate identification of different Chishao sp...
Citations
... and used megaBLAST (in BLASTN v2.2.29; Camacho et al. 2009) using default parameters to align the reads against a nucleotide database (at a 95% similarity threshold). For taxonomic assignment, we compare the ESVs representative sequences with the output reference database (BLAST files) in MEGAN v6.24.16 (Huson et al. 2016;Huson 2023) following set criteria parameters: minimum score = 50, maximum expected = 0.01, top percent = 10, minimum support percent = 0.01, minimum support = 1, and weighted lowest common ancestor algorithm (Xu et al. 2022). We performed a manual taxonomic check to verify that each reference sequence in GenBank corresponded to taxa that have potential distribution in the Neotropics. ...
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... The DNA of the TCPMs used in this study was found to be highly degraded. A mini-barcode, using a sequence (100-250 bp) that was substantially shorter than the original barcode length, was developed to increase the PCR amplification success rate in the DNA-degraded samples (Yang et al., 2022). In this study, the mini-barcode designed according to ITS2 sequences successfully amplified the target fragments from 18 TCPM samples and identified the specific species of rhubarb contained in them. ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/964197974290432-1606894119079_Q64/Xiaolei-Yu-5.jpg)
