February 2002
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10 Reads
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Publications (3)
November 1999
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37 Reads
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83 Citations
Microbiology and Immunology
An amphotericin B (AmB)-resistant mutant was isolated from a wild-type AmB-susceptible strain of Aspergillus flavus by serial transfer of conidia on agar plates containing stepwise increased concentrations of AmB up to 100 microg ml-1. The acquired resistance of mycelia was specific for polyene-antibiotics AmB, nystatin and trichomycin. Spheroplasts derived from the resistant mycelia were as susceptible to AmB as the wild-type. Chemical analysis of the cell wall revealed that levels of alkali-soluble and -insoluble glucans were significantly higher in the resistant mycelia as compared to those in the wild-type. When resistant mycelia were treated with SDS, they adsorbed as much AmB as wild-type mycelia. These results suggest that alterations in the cell wall components of mycelia, especially 1,3-alpha-glucan and protein complex in the outermost wall layer, lead to AmB resistance in A. flavus.
February 1999
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14 Reads
Citations (1)
... Some reports indicate that alterations of the fungal cell wall show a correlation with amphotericin B resistance. In another study, an experimentally evolved A. flavus isolate was able to grow at concentrations of up to 100 µg/mL and the authors assumed that alterations in the cell wall contributed to the resistance [94]. In addition, preclinical and clinical studies showed that amphotericin B is a poor therapeutic option (96% mortality) for A. terreus isolates as it is intrinsically resistant [95], with most isolates exhibiting MIC values ≥ 2 mg/L [96]. ...
- Citing Article
November 1999
Microbiology and Immunology