Hidemi Kumai's research while affiliated with Kindai University and other places

Publications (107)

Article
This two-factor experiment incorporating three photoperiods (12L:12D, 16L:8D and 24L:0D) and two feeding methods (manual and self-feeding) was carried out to determine their effect on the growth performance of striped knifejaw, Oplegnathus fasciatus. Thirty juveniles were stocked into each 300 L tanks in triplicate for each treatment and fed a comm...
Article
This study investigated the influence of initial swimbladder inflation (ISI) failure on survival of Pacific bluefin tuna (PBT), Thunnus orientalis, larvae to prevent mass mortality due to sinking death. In Experiment 1, swimbladder inflation frequency and survival within an ISI promoted (PS) group, for which surface film of rearing water was remove...
Article
This study examined the influence of swimbladder inflation (SBI) failure on mortality (Experiment 1), lordotic deformity (Experiment 2) and growth (Experiment 1, 2) in Pacific bluefin tuna, Thunnus orientalis, postflexion larvae and juveniles by producing larvae lacking inflated swimbladders. Experiment 1 was conducted for postflexion larvae to juv...
Article
This study investigated the optimal timing of day to promote initial swimbladder inflation (ISI) for improved Pacific bluefin tuna (PBT), Thunnus orientalis, larval survival. Larval swimbladder inflation frequency was compared based on three experiments using different time schemes of surface film removal (SFR) from 3 to 9 days post hatch (dph). SF...
Article
Juvenile Pacific bluefin tuna (Thunnus orientalis; PBT) often experience high mortality during ship transportation. This study investigated whether the addition of colours or patterns to the walls of tanks affected survival rate. In the first experiment, three colours and lattice patterns were tested: dark blue single-colour, red single-colour, and...
Article
Initial swimbladder inflation (ISI) of Pacific bluefin tuna (PBT), Thunnus orientalis, larvae was studied to increase the survival of cultured larvae. Experiment 1 was conducted to explore promotion and inhibition of ISI under different water surface conditions; including the use of surface skimmer to remove autogenous surface substances (SS), cove...
Article
Morphogenesis is most active during fish embryonic development with increased susceptibility to environmental stressors. In order to elucidate the causalities of malformations in fishes that produce buoyant eggs, this study estimated the time change of dissolved oxygen (DO) and carbon dioxide (DCD) concentrations in the interstitial water of red se...
Article
AFLP, amplified fragment length polymorphism screening, was conducted to identify sex characteristic DNA markers of cultured Pacific bluefin tuna (PBT), Thunnus orientalis. The AFLP screening of 4 females and 4 males with 64 primer combinations produced a total of 1038 scorable bands, of which 183 were polymorphic between individuals. Male characte...
Article
We cloned the prolactin (PRL) and somatolactin (SL) genes of Pacific bluefin tuna (PBT: Thunnus orientalis) and investigated their daily mRNA expression patterns under aquacultured conditions. The PRL and SL cDNAs of PBT encode 211 and 231 aa prehormones which give rise to 187 and 207 aa mature peptides, respectively, and which belong to the same c...
Article
Bluefin tuna maintain a higher body temperature than ambient sea water. Body heat is derived mainly from metabolic heat to elevate and maintain regional body temperature that is higher than the ambient, while heat loss is caused by heat transfer throughout the whole body surface and gills. Retention of high body temperature is thought to differ at...
Article
The daily expression profile of growth hormone (GH) mRNA in juvenile Pacific bluefin tuna (Thunnus orientalis) was investigated under aquacultured conditions. Total RNA from pituitaries (n = 5) was sampled from 10 am to 12 pm the next day at 1 h intervals. The expression levels of GH mRNA were evaluated using real-time PCR normalized against the β-...
Article
An inhibitory effect of Kunitz and Bowman-Birk soybean trypsin inhibitors (K- and BB-SBTI) was investigated on tryptic and chymotryptic proteinases of a brood of tiger puffer Takifugu rubripes and yellowtail Seriola quinqueradiata. These assist to comprehend the differences in dietary soybean meal (SBM) utility by fish species and to develop low co...
Article
Hatching rates and hatching times (50% hatch) of red sea bream, Pagrus major (Temminck & Schlegel), eggs in culture media that were prepared to dilute rotifer Brachionus plicatilis Muller extract at 4000-, 6000-. 8000- and 10 000-fold with artificial sea water (σ15, = 24.0) were investigated. Although differences in hatching rate between the cultur...
Article
Thyroid hormones (THs) are essential for the embryonic and post-embryonic development of fish. We studied the role of THs during the early, post-embryonic, development of Pacific bluefin tuna. Embryos were treated with L-thyroxine (T(4)) or the anti-thyroid drug methimazole (MMI), and reared in microtitre plates for 3 days. Immersion in MMI, but no...
Article
In bluefin tuna aquaculture, collision of juveniles with the tank or net walls is a major cause of high mortality. This problem may be related to color sensibility of the visual mechanisms of this species. As a first step in understanding of color vision of Pacific bluefin tuna Thunnus orientalis, we applied a molecular technique and histology to s...
Article
We studied the profiles of 3,5,3'-l-triiodothyronine (T3), thyroxine (T4), and thyroid hormone receptors (TRs) in Pacific bluefin tuna (Thunnus orientalis) during embryonic and post-embryonic development. Both T3 and T4 were detected in embryos just before hatching, and it was found that the levels of both were increased in postflexion fish. The th...
Article
Previous studies have shown that feeding enriched Artemia induces growth failure in Pacific bluefin tuna (PBT) Thunnus orientalis larvae. This growth failure cannot be improved even if the docosahexaenoic acid (DHA) content in enriched Artemia is elevated to the same level as that in yolk-sac larvae, an ideal live feed for PBT larviculture. This mi...
Article
Development of bluefin tuna farming in Japan has a 30-year history. This paper reviews recent developments as well as the current status and problems of farming, broodstock management, and fingerling production in Japan. Farmed bluefin tuna by wild seed-stock comprises approximately 15–20% (2,400 tons) of the annual catch of bluefin in Japan. Compa...
Article
Three photoperiods (12L:12D, 16L:8D and 24L:0D) were used to investigate the growth performance and stress response in red sea bream, Pagrus major (body weight 200–400 g). Fish were fed a commercial diet to apparent satiation, two times a day for 8 weeks. Fish exposed to a 24L:0D photoperiod showed a significantly higher weight gain (%) than those...
Article
A biological1y interesting lipid deposition system in tunas enables them to migrate a long distance in the ocean. On the other hand, the condition of lipid deposition in their meat is important to evaluate their meat quality as the human food. This study investigated the ontogenetic change of neutral lipid deposition in reared larval and juvenile P...
Article
Stable reproduction is essential for supplying artificially hatched fish to tuna aquaculture. We observed testes maturation in reared Pacific bluefin tuna (PBT) Thunnus orientalis at 2+ years of age. The incidence of males with mature testes was 25.0%, and 40% of the males had developing testes that contain spermatozoa, while oocytes of the same ag...
Article
Feeding enriched Artemia induces growth failure in Pacific bluefin tuna (PBT) Thunnus orientalis larvae; however, feeding of yolk-sac larvae of marine fish promotes larval growth. It is considered that this growth failure partly results from dietary docosahexaenoic acid (22:6n-3, DHA) deficiency. Therefore, we examined the effect of DHA contents in...
Article
The ontogenetic changes in the growth potential of larval and juvenile laboratory-reared Pacific bluefin tuna were examined based on RNA–DNA and protein–DNA ratios. Experimental fish were reared at the Ohshima Experiment Station of Kinki University Fisheries Laboratory in August 2002. Samples were taken from 13 to 35 days after hatching (DAH). Meta...
Article
Transgenic technology has been widely applied to a variety of freshwater fish species. However there are few reports on the use of this technology in commercially important marine species. In this study, the construction of expression vectors containing the β-actin promoter region for use in the red sea bream Pagrus major, a species of considerable...
Article
— Indoor and outdoor experiments were conducted to improve the rearing techniques for artificially hatched northern bluefin tuna Thunnus thynnus during growout culture. Collisions with the walls of tanks or nets caused mass mortality that occurs during growout. The period when collisions frequently occur and the types of injury caused by collision...
Article
The efficacy of immersion-vaccination against gliding bacterial disease in red sea bream Pagrus major was examined with formalin killed cells (FKC) of Tenacibaculum maritimum. FKC was prepared from T maritimum type strain R2 grown on modified Cytophaga agar medium containing 70% sea water for 24 h at 25 degrees C by inactivating with 1.5% (v/v) for...
Article
Two consecutive feeding trials were conducted to know: (i) how the apparent digestibility coefficient (ADC) varies among replicates on different days and during the course of the experiment (Trial 1); and (ii) the effect of stocking density (e.g. 7.17, 5.56, and 3.92 kg fish/t water) on the ADC of nutrients and energy in red sea bream (Trial 2). In...
Article
This study was carried out to investigate the suitability of Artemia enriched with docosahexaenoic acid (DHA) and choline as live food on the growth and survival rate of the Pacific bluefin tuna (PBT; Thunnus orientalis) larvae. The PBT larvae were fed either Artemia enriched with oleic acid (Diet 1), DHA (Diet 2), DHA+choline 1.0 mg L−1 (Diet 3) a...
Article
Using the full-cycle cultured (FC) Pacific bluefin tuna [body weight 16.3 ± 1.9 kg (prefasting group, pre-FG), 14.2 ± 0.9 kg (post-fasting group, post-FG)], changes in the physical/chemical properties of the cephalal parts of dorsal (D) and ventral (V) ordinary muscles (OM) by fasting (6 days) during chilled storage (4°C) were investigated. Conditi...
Article
Juvenile of red sea bream (Pagrus major, body weight 1∼30 g) were reared under four photoperiods (6L:6D, 12L:12D, 16L:8D and 24L:0D) with constant light intensity 1500 lx on the water surface to investigate their growth performance and stress response. Fish were fed a commercial diet to apparent satiation for 8 weeks of the experiment. Significantl...
Article
A cDNA encoding transthyretin was cloned from the Pacific bluefin tuna (Thunnus orientalis). This cDNA contains a complete open reading frame encoding 151 amino acid residues. The deduced amino acid sequence is 81% and 55% identical to the gilthead seabream and common carp forms, respectively, and 33-39% to mammalian, reptilian, and amphibian forms...
Article
Gustatory neural responses of the Pacific bluefin tuna Thunnus orientalis (Temminck and Schlegel) to extracted compounds of prey organisms, such as amino acids, nucleotide-related substances, organic acids and organic bases, were electrophysiologically recorded from the facial nerve supplying the anterior palate. Of the 17 amino acids tested, l-pro...
Article
A previous study elucidated that an extreme hypoxia during somitogenesis induced the most frequent skeletal malformation centrum defects in red sea bream (RSB), Pagrus major. In this study, details of the hypoxic conditions to induce them in RSB, dissolved oxygen (DO) concentration and exposure time to hypoxia, were investigated. Fertilized eggs we...
Article
Artificially hatched Seriola species have the problem of malformation, mainly in their vertebrae, head, and mouth parts. To clarify the cause of vertebral malformation, the effects of hypoxia during embryogenesis on the induction of centrum defects was investigated in artificially hatched amberjack Seriola dumerili. Firstly, 7-somite stage embryos...
Article
A number of primary and secondary features used as stress indicators in fish were investigated in red sea bream, Pagrus major exposed to different photoperiod regimes. In Experiment 1, fish weighing from 17 to 25 g were subjected to acute stress consisted of netting the fish from the rearing tanks and confinement in a small tank (30 L) half-full of...
Article
Human melanocytes respond to UV irradiation by increasing the synthesis of melanin. While much is now understood of the pathways governing this process and the nature of the melanin synthesized, little is known of melanins produced by lower vertebrates and their capacity to respond to UV. Here we report that a fish, red seabream, can undergo 'sunta...
Article
Two consecutive trials were conducted to investigate the effects of photoperiod manipulation on growth rate, food intake and feed conversion efficiency (Trial 1), and the digestibility of nutrients and energy (Trial 2) in red sea bream, Pagrus major (body weight 19–120 g). Fish were exposed to four photoperiods (6L:6D, 12L:12D, 16L:8D and 24L:0D) w...
Article
We observed the spawning of chub mackerel Scomber japonicus eggs under a light microscope in 2002. The chub mackerel were kept at the Fisheries Laboratory, Kinki University, Shirahama Experimental Station since 1999. Mature males and females (BL: 33.5±2.3 cm BW: 577.9±144.8g, n=50) were kept in 50 ton spawning tank between May to July. A total of 8...
Article
Tuna aquaculture is currently dependent on the wild capture of juveniles for production. The development of hatchery technology for bluefin and other tunas would be a major step forward in improving sustainability of their aquaculture. The present study overviews the technology in the life cycle completion of the Pacific bluefin tuna (PBT) Thunnus...
Article
A histological examination was made of the ontogenetic development of the digestive and immune systems of the larval and juvenile kelp grouper Epinephelus bruneus reared in the laboratory. The liver, gall bladder, pancreas and the demarcating region between the intestines and rectum were formed within 3 days post-hatch (dph). During the preflexion...
Article
Restriction proteolysis of the yolk proteins during the final oocyte maturation appears to result in the low content of free amino acids (FAA) and the unique FAA profile in Japanese eel Anguilla japonica eggs obtained by hormonal inducement.
Article
Vertebral deformities in red sea bream, Pagrus major, remain serious obstacles to the improvement of seedling quality for its aquaculture. However, the causalities of the deformities remain unclear and prevention methods have not yet been established. In this paper, oxygen deficiency during somitogenesis was demonstrated to cause centrum defects (f...
Article
Vertebral deformities were investigated in cultured red sea bream, Pagrus major. In the field, deformities in seedlings were categorized and their incidence was calculated. In the laboratory, the symptoms of major vertebral deformities were examined morphologically using radiographs and by making transparent skeletal specimens. The internal structu...
Article
The gonadal sex differentiation in red sea bream, Pagrus major, which is one of the most important species for aquaculture in Japan, was revealed histologically. The suitable conditions for induction of all-male groups in the fish were investigated, and functional males were induced by the conditions of oral administration of 17a-methyltestosterone...
Article
Eggs of the Japanese eel, Anguilla japonica, were obtained by hormonally induced maturation and ovulation, and then categorized as buoyant and non-buoyant types in seawater after insemination. The water content of the buoyant eggs, 93.1%, was significantly higher than that of the non-buoyant eggs, 87.5%. Furthermore, the buoyant eggs had 1.5 times...
Article
Changes in tolerances to certain environmental stressors, e.g., low and high temperatures and salinity as well as high concentrations of ammonia, were studied in the red sea bream Pagrus major during its early life stage up to 42 days post-hatch. Relative tolerance was assessed by determining 24-h median lethal level. In laboratory experiments, met...
Article
: Nile tilapia were exposed to severe hypoxic conditions. The respiratory frequency (RF) of the fish reached a maximum level at approximately 10 mmHg PO2 and decreased gradually thereafter. The fish sank to the bottom and subsequently their respiration began to stop. The hypoxic conditions increased the hematocrit value. As RF decreased, plasma cor...
Article
Gustatory response of marbled rockfish Sebastiscus marmoratus was recorded from the facial nerve supplying the anterior palate. In amino acids, the lowest threshold was for L-proline at 10−6–10−5 M; in ATP-related substances, the value for inosine was approximately 10−5 M and in organic acids, the value for L-lactic acid was approximately 10−6 M. A...
Article
Red sea bream, Pagrus major, is one of the most important fish cultured in Japan. Two clones of red sea bream were produced. Eggs from a mitotic gynogenetic diploid (mitotic-G2N) red sea bream were inseminated either with sperm from a mitotic-G2N male to produce a heterozygous clone (hetero-clone), or with UV-irradiated sperm of Japanese parrot fis...
Article
Morphological development and growth of larval and juvenile Pacific bluefin tuna, Thunnus thynnus, were studied from laboratory-reared specimens. Average body length (BL) of newly hatched larvae was 2.83 mm and larvae grew on average 5.80 mm by 10 days, 10.62 mm by 20 days, and 35.74 mm by 30 days after hatching. Growth was especially accelerated a...
Article
The pigmentation of a new backcross sea bream (RF1), red sea bream Pagrus major (RSB) ♀ x F1 (RSB ♀ x crimson sea bream Evynnis japonica ♂) ♂, weighing 265 g on an initial mean body weight, was compared with RSB, weighing 280 g on an initial mean body weight, by a 30-day-feeding trial. The FI were initially bred as a new hybrid, owing to the improv...
Article
A new backcross sea bream (RF1), red sea bream (RSB) Pagrus major 5 x F1 cf (RSB 5 x crimson sea bream Evynnis japonica ♂7), and RSB, weighing 107 g on an initial mean body weight, were given a commercial diet at a daily feed intake of 1.2% body weight for 80 days. The RFi steadily grew but showed lower final mean body weight, feed efficiency, prot...
Article
Red sea bream Pagrus major, red sea bream ♀ x crimson sea bream Evynnis japonica ♂ (F1), and red sea bream ♀ x F1 ♂ (RF1), with body length (BL) of 35-41 cm and 2-5-year-old, and crimson sea bream, with BL of 25-33 cm and 8-year-old, in attempt to develop new sea bream seedlings, were compared with their muscle fiber diameters. These fishes were fe...
Article
Juvenile red sea bream, Pagrus major, having an initial mean body weight of 40g, were given on a fish meal diet to satiation under four rearing protocols, two mean water salinities of 31.9 and 23.8 (32s and 24s) by two initial rearing densities of 2 and 4 kg/m³ (2d and 4d), at a mean water temperature of 21.2°C for 20 days. Another two groups of th...
Article
Full-text available
Wild young bluefin tuna, Thunnus thynnus, caught in 1987, were cultured up to be adults. From 5 years old, presumably the fish became mature, the seasonal change of their gonado-somatic index (GSI) was examined. From June to August every year, their spawning condition was observed. In addition, the gonad condition was examined during the spawning s...
Article
Full-text available
Rotifer-size selectivity and the optimal feeding density were examined for reared bluefin tuna larvae. Larvae were obtained from the broodstocks raised for 11 years in captivity. Larvae were fed rotifers at 5 different densities, 5, 10, 20, 30, or 40 ind./ml, on the day of the commencement of feeding (3 days after hatching) and the next day. In the...
Article
An inhibitory effect of Kunitz and Bowman-Birk soybean trypsin inhibitors (K- and BB-SBTI) was investigated on tryptic and chymotryptic proteinases of a brood of tiger puffer Takifugu rubripes and yellowtail Seriola quinqueradiata. These assist to comprehend the differences in dietary soybean meal (SBM) utility by fish species and to develop low co...
Article
The growth and morphological development of larval and juvenileEpinephelus bruneus were examined in a hatchery-reared series. Average body length (BL) of newly-hatched larvae was 1.99 mm, the larvae growing to an average of 3.96 mm by day 10, 6.97 mm by day 20, 12.8 mm by day 30, 22.1 mm by day 40 and 24.7 mm by day 45 after hatching. Newly-hatched...
Article
Soybean trypsin inhibitors (SBTI) inhibited trypsin and basic proteinase in the hapatopancreas, pyloric ceaca and intestine of cultured-marine and freshwater fishes. The highest trypsin inhibition by the SBTI was obtained in tiger puffer Takifugu rubripes, followed by rainbow trout Oncorhynchus mykiss, striped jack Pseudocaranx delicatissimus, red...
Article
Naturally fertilized bluefin tuna Thunnus thynnus eggs which were obtained from 7-year-old broodstock in a net cage were incubated until hatching at 27°C. No conspicuous changes were found in egg moisture and amounts of total nitrogen and phospholipid during the embryonic development. Egg protein gradually increased, but free amino acid decreased w...
Article
The tiger puffer Takifugu rubripes (puffer) and the red sea bream Pagrus major (bream) were fasted or fed 1/4, 1/2, and 1 satiation of an artificial diet for 20 days, and digestive and absorptive ability and energy flow were composed. The puffer fed 1/2 and 1 satiation of the diet showed higher feeding rate, growth rate, feed efficiency, protein ef...
Article
Relative values of hepatopancreas and intestinal weight to somatic weight were significantly higher in the tiger puffer Takifugu rubripes (puffer) than the red sea bream Pagrus major (bream). Relative values of intestinal length to body length were reversely high in the bream, indicating that the puffer have intestines with large diameters. Feces o...
Article
Changes in chemical constituents and enzyme activities were assayed in red sea bream Pagrus major through embryonic development from eggs immediately after fertilization to larvae just before feeding. No significant changes were found in protein, total nitrogen, and phospholipid contents with proceeding development stages. Although free amino acid...
Article
We attempted to improve the breed of red sea bream Pagrus major by means of selective breeding in order to establish red sea bream seedling for aquaculture capable of growing more quickly than natural red sea bream. In about 1964, natural young fish were reared into broodstock and, among young fish obtained from these broodstock, those growing quic...
Article
Tiger puffer Takifugu rubripes, weighing about 10g, were reared for 28 days onbrown fish meal diets containing 10, 13, 16, 19, and 22% white dextrin. Except for a low survival (75%) obtained in the fish reared on the lowest whitedextrin diet, the fish reared on other diets and sandeel, as a reference, showed high survival (>95%). Under a similar da...
Article
Feeding stimulants for the tiger puffer Takifugu rubripes were identified by omission test using the synthetic clam Tapes japonicus extract, based on a daily feeding rate for casein diet with each test solution. The amino acid fraction showed remarkably higher feeding stimulant activity than those of nucleotide and other chemical fractions in the e...
Article
Tiger puffer Takifugu rubripes, weighing about 3.7g, were reared for 20 days on brown fish meal diets with graded levels of pollack liver oil, 0, 2.5, 5.0, 7.5, and 11.0%. The best feed efficiency, protein efficiency ratio, and apparent protein retention were obtained in the group fed the diet with 7.5% oil. Otherwise, there appeared to be no signi...
Article
In order to obtain basic knowledge for promoting seed production of the striped jack Caranx delicatissimus, chemical composition, nucleic acid contents, and alkaline phosphatase activity (ALP) were measured in the fish, from larvae through juveniles at 1-3 day intervals. While rearing on rotifer, Artemia nauplii, and an artificial diet in that orde...
Article
The qualitative requirements and deficiency signs of water-soluble vitamins were studied in tiger puffers Takifugu rubripes having mean body weight of 2.9g. The qualitative requirements of tiger puffer for water-soluble vitamins indicate that the requirement of this species for vitamin B6 is lower than that of other fish species such as red sea bre...
Article
A 42-day feeding trial was conducted with young yellowtails (163.6g) for the purpose of improving undesirable hematological characteristics caused by feeding a soy protein concentrate (SPC) diet (moist type pellet). Zero, 50, 100, 250, 500, and 1000mg of iron proteinate (Fe-P; 10-13% Fe) were respectively added per each 100g of the SPC diet, contai...
Article
Changes in alkaline phosphatase (ALPase), glucose-6-phosphate dehydrogenase (G6PDH), and acidic and basic protease (Acid- and Basic-ease) activities of red sea bream Pagrus major, from larvae to juveniles of five lots, were assayed at 3-7 day intervals until 49 days post-hatch. The enzyme activities expressed as per individual fish were all increas...
Article
Control and stressed groups of juvenile Japanese parrot fish Oplegnathus fasciatus were fed diets (moist pellet) supplemented with 0, 75, and 300mg of ascorbic acid (AsA) per 100g. Fish in the stressed group were intermittently exposed to decreasing water oxygen levels every 3 or 4 days for 16 weeks. In the fish given a diet containing no AsA suppl...
Article
The capability of planktonic yolk-sac larvae of red sea bream Pagrus major in detecting food was examined in the laboratory to ascertain basic knowledge on the early life history of this marine fish. After infrequent vertical burst swimming followed by slight rising or sinking, the larvae remained motionless within thin layers of concentrated food...
Article
A croaker (Nibea mitsukurii) has a high incidence of the skin melanoma, chromatophoroma, in a Pacific coastal area in Japan. A sea catfish (Plotosus anguillaris) bearing skin melanosis is also found in the same area. For elucidation of a correlation between these pigment cell neoplasms of the skin and environmental contaminants, an epidemiological...
Article
Feeding stimulants for the marbled rockfish Sebasticus marmoratus were identfied by the omission test uding the synthetic extract of krill Euphausia pacifica. The fish were fed on a purifiedcasein diet supplemented with constituents of the extract for 6 days. The feeding stimulant activity was evaluated by the daily feeding rate. The nucleotide fra...
Article
Chromatophoroma in the croaker (Nibea mitsukurii) showed a unique geographic distribution. The contribution of environmental chemicals to the cause of chromatophoroma in the feral croaker is considered likely on the basis of the following results in our studies. 1) Chromatophoroma was induced in tank-reared N. mitsukurii by administration of certai...
Article
The digestive system in the ocellate puffer was histologically and biochemically examined. Also, the effect of scallop extract as a flavor in a diet on performance of the puffer was investigated. The ocellate puffer lacks a stomach, resulting that its expansion sea observed only esophageal epithelium and expansion sac-epithelium and that pepsin-lik...

Citations

... These results coincided well with the reported morphological development that body constitution and physiologiccal functions were basically accomplished immediately before and/or at the shift point from larvae to juveniles of the striped jack.10) Takii et al. 19,20) reported that both CP and ASH contents and ALP activity increased rapidly from the first feeding up to the morphological shift point to juveniles of the red sea bream, about 10 mm TL. It has already been suggested in mammals that ALP functions as an inorganic phosphate transfer of the matrix vesicle in relation to bone formation and that its activity was also detected in the intestine, liver, and kidney.21) ...
... A same basal diet with approximately 50% protein and 8% lipid, which were demonstrated to be suitable for both turbot and tiger puffer [22][23][24][25], was used for these two species. The basal diet (control) was made with a medium fishmeal level (30%, [26][27][28][29]) but no fish oil (Table 1). ...
... The active constituent in the amino acid fraction was identified as the fraction glycine, alanine, proline plus histidine whose activity was found to be comparable to that of the complete synthetic extract. A similar methodology was used by Takaoka et al. (1995) who studied feeding stimulants for the tiger puffer Takifugu rubripes testing synthetic extract of clam Tapes japonicus. The amino acid fraction showed remarkably higher feeding stimulant activity than those of nucleotide and other chemical fractions in the extract. ...
... The subsequent bioinformatics screening against torafugu genome illustrated the potential disconnection between torafugu FoxO1A and carbohydrate metabolism, where GLUT4 and PYG did not contain any DBEs in their upstream regions analyzed (within 500 nt). Since the expression of these genes is regulated by FoxO1 in mammals (Armoni et al., 2007;Karnieli and Armoni, 2008;Melnik, 2010;Süren-Castillo et al., 2014;Kinoshita et al., 2016), these results may be associated with the low carbohydrate requirement in torafuguthe optimal carbohydrate content of torafugu diet is about 20% (Takii et al., 1995), whereas rodent diets often contain more than 50% of carbohydrate (National Research Council, 1995). Further investigation on the FoxO1A and its target genes will shed new light on the long-standing challenge in aquaculture to increase the carbohydrate utilization efficiency of fish. ...
... Specifically, according to the total length, the greater amberjack reared in the P. inhibens-enriched water may have initiated the stage of metamorphosis earlier in comparison to the control group. The relative contribution of hyperplasia in the growth procedure decreases with standard fish length, as reported by Zimmerman and Lowery [87]. Therefore, the apparent decrease in p38 MAPK, p44/42 MAPK, and JNK activation may indicate that P. inhibens favored a more rapid growth, potentially leading greater amberjack to advanced development, where the hyperplastic-facilitated procedures were mostly completed. ...
... On the other hand, our recent observations in juvenile red sea bream (0.1 g) fed Artemia nauplii with different level of proteins showed that a portion of EI as M ranged from 30 to 35%, depending on the highly-unsaturated fatty acids (HUFA) composition of the nauplii used (Sumule et al., 2003). Moreover,Takii et al. (2000)reported that EI channeled to M is higher (55–58%) in higher salinity (32 psu) compared to that of lower salinity (58–63%, 25 psu). It is likely that the proportion of EI allocated for metabolism was affected by the size of fish, protein content of diet and environmental conditions (DeSilva and Anderson, 1995). ...
... Fish fed PF showed a decrease in survival after 8 days of the beginning of the feeding trial compared to those fed a formulated diet. According to Sawada et al. (2000) during hatchery production, PBT larvae are fed yolk-sac larvae such as spangled emperor fish Lethrinus nebulosus or Japanese parrotfish Calotomus japonicas until they reach 10 to 40 mm of total length. Then, they were supplied with minced sand lance Ammodytidae personatus from 40 mm total length onward. ...
... Due to the increasing demand from consumption, the tuna stocks are considered to be at risk of overexploitation, although it previously had been thought that tuna harvesting would never cause their populations to become threatened 20,23 . In response to the risk of over fishing, the aquaculture programme of the Pacific bluefin tuna started in 1970 in Japan, resulting in predominantly farm-raised fish by 2002 24,25 . Despite this innovative achievement, most tuna aquaculture business still depends on wild stocks of juveniles because the survival rates of larval and juvenile tuna from artificial reproduction is not high 26 . ...
... New Zealand Journal of Marine and Freshwater Research, 2009, Vol. 43 To our knowledge there is no published information on the nutritional requirements of yellowtail kingfish, but there are several studies on the effect of different dietary formulations for Japanese yellowtail (S. quinqueradiata) and Mediterranean yellowtail (S. dumerili) (Takii et al. 1989;Ukawa et al. 1994;Masumoto et al. 1996;Ruchimat et al. 1997a,b;Watanabe et al. 2000a;Tomás et al. 2005;Vidal et al. 2008). Most of these studies relate to the optimisation of protein inclusion rates, or substituting fish meal with other animal or plant protein sources. ...
... This may be due to the fact that AA reduces nitrite in the blood because of its antioxidant properties and is not related to a reduction in stress. Tolerance of Japanese parrot fish, Oplegnathus fasciatus, and spotted parrot fish, O. punctatus, to low dissolved oxygen appeared to be improved as dietary AA increased (Ishibashi et al. 1992). Gilthead seabream, Sparus aurata, fed an AA-free diet showed a higher concentration of plasma glucose at 3 hours after hypoxia stress and a higher concentration of plasma cortisol at 9 hours after hypoxia stress than fish fed diets containing AA (Henrique et al. 1998). ...