H. Huang's research while affiliated with Chinese Academy of Sciences and other places
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Publications (3)
Rhizopus currently comprises 10 species two of which, R. arrhizus and R. microsporus, are divided into nine varieties. The molecular phylogenetic relationships among varieties were studied here by using IGS rDNA sequences. All three varieties of R. arrhizus were characterized by special short tandem repeat (STR) motifs except for strain CBS 257.28....
DNA sequences from two loci, the entire ITS rDNA and a portion of pyrG, were examined to study relationships within Rhizopus morphospecies. The ITS rDNA sequences from 55 strains representing all 10 currently recognized species showed great differences in length among species ranging from 450 to 788 bp. Phylogenetic analysis of the ITS rDNA resolve...
The internal transcribed spacers of ribosomal DNA (rDNA ITS) of 20 strains representing 12 species of the genus Cunninghamella were amplified using the polymerase chain reaction (PCR) with a pair of oligonucleotide primers ITS1 and ITS4. The PCR products were cloned and sequenced. The entire lengths of the rDNA ITS region of the 20 strains range fr...
Citations
... The genus Rhizopus currently comprises ten species, some of which are morphologically heterogeneous. For the two prevalent species, R. arrhizus (= R. oryzae) and R. microsporus, several varieties are currently accepted[21]. R. ...
... In contrast to Rhizopus (Liu et al. 2008, Abe et al. 2010, Dolatabadi et al. 2014a, Lichtheimia (Alastruey-Izquierdo et al. 2010), and Apophysomyces (Álvarez et al. 2010) where modern species concepts were applied based on molecular phylo-genies, the species-rich, polyphyletic genus Mucor lacks a state-of-the-art taxonomy. Recent taxonomic studies are limited to species occurring in particular habitats, such as clinical samples (Álvarez et al. 2011) or cheese (Hermet et al. 2012), and consequently do not cover the complete diversity of the fungi concerned. ...
... However, C. clavata is distinguished from C. verrucosa by broken pedicels on the surface of vesicles (absent vs. present), shape of vesicles (typically clavate, sub-ovoid to irregular vs. usually clavate and oval, sometimes subglobose), and branched sporophores (3-4 arranged pseudo-verticillate vs. unbranched or simply branched) Chen 1998, 2001). Zheng and Chen (2001) studied the taxonomy of Cunninghamella using 197 strains from around the world, accepted 15 taxa (12 species and three varieties) on the basis of pattern of sporophores, shape and size of vesicles, size of sporangiola, maximum growth temperature, mating compatibility and molecular data (Liu et al. 2001, Zheng andChen 2001). Number of species in Cunninghamella have remained constant for 14 years until six species were described using molecule and morphology (Guo et al. 2015, Hyde et al. 2016, Zhang et al. 2020, Hallur et al. 2021, Suwannarach et al. 2021, Zhao et al. 2021b). ...