Gustavo Zamberlam's research while affiliated with Université de Montréal and other places

... Mechanistically, DUSP16 impedes JNK and p38 activation, thereby attenuating BAX accumulation within mitochondria and subsequently retarding apoptosis progression [27]. In mice, DUSP1 (MKP-1) exerts a negative regulatory role in immune cells and is closely associated with the immune response and inflammation [28]. Simultaneously, DUSP16 also participates in the regulation of nervous system development and embryonic development [29]. ...

... Among other things, they discovered that the adrenal gland of the dog has an intrinsic osmosensitivity [19, 21-23, 31, 32]. A number of transgenic mouse models have been developed to study the development and pathophysiology of the adrenal gland [1,2,4,6,12,15]. The phenotype of these mice is usually studied at the animal level using in vivo methods, clinical chemistry methods, histologic techniques, fresh adrenal gland slices, and primary cultures. ...

... 29 More recently, we also showed that inactivation of Lats1 and Lats2 in fetal Leydig and Sertoli cells of the developing testis impairs testicular development. 30 However, this model could not determine whether the abnormal phenotype observed in Sertoli cells and fetal Leydig cells resulted from a direct effect of Lats1/2 inactivation in these cells, an indirect effect of these cell populations on each other, or more likely, a combination of both. ...

... YAP1/TAZ has been revealed to regulate ovulation in bovines [15], be associated with pregnancy recognition and establishment in ewes [31], and be involved in embryonic development in cows [32]. These studies have opened the prelude to the study of YAP1/TAZ in ruminants. ...

... [31] Yap/Taz [32] Lats1/Lats2 [33] Mst1/Mst2 [34] FAdE/Nr5a1-Cre [35] Fetal cortex Tracing fetal adrenocortical cells descendants [35,36] FAdE/Nr5a1-CreERT2 [35] Fetal cortex Prkar1a [37] Tracing fetal adrenocortical cells descendants [35,37] Nr5a1 eGFP-CreERt2 -AGP/fetal cortex, definitive cortex hCyp11a1-iCre [38] Fetal cortex/definitive cortex Insr/Igf1r [39] mCyp11a1-iCre [40] Fetal cortex/definitive cortex Ctnnb1 ex3 [41] Nr5a1 [40] Cyp11a1 Gfp,Cre/+ [42] Fetal cortex/definitive cortex AR [43,44] Akr1b7-Cre [45] Fetal cortex/definitive cortex Ctnnb1 ex3 [24,25,46,47] Prkar1a [25,26] Prkaca [25] Cyp11b2 Cre /AS Cre [48] Aldosterone producing zG cells and their zF descendants ...

... A number of studies indicate the importance of Hippo effectors to ovarian physiology in adult animals, including cattle [14][15][16]. Interestingly, it has been shown that overexpression of YAP negatively impacts LH action in mouse granulosa cells (GCs) [17] and that YAP is considered a susceptibility gene for polycystic ovarian syndrome in women [18,19]. ...

... Inhibition of the Hippo signaling pathway inhibits GnRHinduced ovulation in cattle, indicating that this pathway indeed plays a crucial role in animal ovulation [36]. Additionally, the Hippo signaling pathway might regulate the secretion of luteinizing hormone in the mouse pituitary gland, consequently regulating the reproductive process in mice [37]. ...

... GA and VP are commonly used to investigate the functions of YAP1/TAZ in various developmental processes or tumor progressions, such as the proliferation of ovarian cancer cells [12], the reprogramming of colonic epithelium [13], and the homeostasis and repair of skin epithelium [14]. In addition, VP has been successfully used to inhibit YAP1 activity in bovine preovulatory granulosa cells [15]. Thus, GA and VP could be proper tools for studying the function of YAP1/TAZ in RE cell proliferation. ...

... Female reproductive factors, such as ovarian development, oocyte maturation, estrous cycle, and embryo implantation, were influenced primarily by sex hormones, including progesterone, prostaglandin, and estrogen (Innocenti et al., 2017;Bui et al., 2022). Sex hormones play crucial roles in the complex and interconnected processes of pregnancy and childbirth (Zamberlam et al., 2019). Fat could affect the synthesis of cholesterol, which is the precursor of sex hormones (Fox et al., 2009). ...

... Furthermore, inhibition of CTH activity can reduce the ovulation rate in mice. 17 And CTH mRNA has also been reported in porcine cumulus cells and oocytes. 18 Moreover, a decrease in CTH activity may also lead to placental abnormalities in pre-eclampsia and fetal growth restriction. ...