Geun Heo's research while affiliated with Chungbuk National University and other places
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Publications (16)
N6-methyladenosine (m6A), the most prevalent modification in eukaryotic messenger RNA (mRNA), plays a key role in various developmental processes in mammals. Three proteins that affect RNA m6A modification have been identified: methyltransferases, demethylases, and m6A-binding proteins, known as “writer,” “eraser,” and “reader” proteins, respective...
Glucose‐regulated protein 78 (GRP78) binds to and stabilizes melanocortin 4 receptor (MC4R), which activates protein kinase A (PKA) by regulating G proteins. GRP78 is primarily used as a marker for endoplasmic reticulum stress; however, its other functions have not been well studied. Therefore, in this study, we aimed to investigate the function of...
Y-box binding protein 1 (YBX1) plays important roles in RNA stabilization, translation, transcriptional regulation, and mitophagy. However, its effects on porcine preimplantation embryos remain unclear. In this study, we knocked down YBX1 in the one-cell (1C) stage embryo via small interfering RNA microinjection to determine its function in porcine...
Activating transcription factor 6 (ATF6), one of the three sensor proteins in the endoplasmic reticulum (ER), is an important regulator of ER stress-induced apoptosis. ATF6 resides in the ER and, upon activation, is translocated to the Golgi apparatus, where it is cleaved by site-1 protease (S1P) to generate an amino-terminal cytoplasmic fragment....
Zinc finger and SCAN domain-containing 4 (ZSCAN4), a DNA-binding protein, maintains telomere length and plays a key role in critical aspects of mouse embryonic stem cells, including maintaining genomic stability and defying cellular senescence. However, the effect of ZSCAN4 in porcine parthenogenetic embryos remains unclear. To investigate the func...
Heat stress (HS) is a long-standing hurdle that animals face in the living environment. Alpha-lipoic acid (ALA) is a strong antioxidant synthesized by plants and animals. The present study evaluated the mechanism of ALA action in HS-induced early porcine parthenotes development. Parthenogenetically activated porcine oocytes were divided into three...
Y-box binding protein 1 (YBX1) is a member of the family of DNA- and RNA-binding proteins that play crucial roles in multiple aspects, including RNA stabilization, translational repression, and transcriptional regulation; however, its roles in embryo development remain less known. In this study, to investigate the function of YBX1 and its mechanism...
YME1L1, a mitochondrial metalloproteinase, is an Adenosine triphosphate (ATP)-dependent metalloproteinase and locates in the mitochondrial inner membrane. The protease domain of YME1L1 is oriented towards the mitochondrial intermembrane space, which modulates the mitochondrial GTPase optic atrophy type 1 (OPA1) processing. However, during embryonic...
Rotenone is a commonly used insecticidal chemical in agriculture and it is an inhibitor of mitochondrial complex Ⅰ. Previous studies have found that rotenone induces the production of reactive oxygen species (ROS) by inhibiting electron transport in the mitochondria of somatic and germ cells. However, there is little precise information on the effe...
Increased levels of oxidative stress are major factors that drive the process of post-ovulatory oocyte aging. Epigallocatechin-3-gallate (EGCG), which accounts for up to 50% of the catechins, possesses versatile biological functions, including preventing or treating diabetes, cancer, and heart diseases. The aim of this study was to explore whether...
In mammals, E2 factor (E2F) acts as a cell cycle regulator. E2F transcription factor 4 (E2F4) is a member of the E2F family of transcription factors and usually represents predominant E2F activity in cells. The E2F4 gene has been extensively studied in animals and is associated with multiple functions, such as cell cycle regulation and apoptosis; h...
Background:
Activating transcription factor 7 (ATF7) is a member of the ATF/cAMP response element (CRE) B superfamily. ATF2, ATF7, and CRE-BPa are present in vertebrates. Drosophila and fission yeast have only one homologue: dATF2 and Atf1, respectively. Under normal conditions, ATF7 promotes heterochromatin formation by recruiting histone H3K9 di...
Background: Rotenone is a commonly used insecticidal chemical in agriculture and it is an inhibitor of mitochondrial complex 1. Previous studies have found that rotenone induces the production of reactive oxygen species (ROS) by inhibiting electron transport in the mitochondria of somatic and germ cells. However, there is little precise information...
Alpha-linolenic acid is an important polyunsaturated fatty acid that exhibits anticancer, anti-inflammatory, and antioxidative effects. In this study, we investigated the protective effects of alpha-linolenic acid on the cell proliferation and differentiation of C2C12 cells under essential amino acid-deficient conditions. Different concentrations o...
Heat stress (HS) has been known to cause reproductive failure in animals, especially in summer. HS severely affects the developmental potential of oocytes and leads to low fertility rates. Previous studies have reported that HS compromises embryo development in bovine oocytes, and reduces ovarian development in mice, thereby impairing reproductive...
Citations
... 8 YBX-1 also plays an important role in regulating cell growth and embryonic development. 9 Moreover, YBX-1 regulates self-renewal and neuronal differentiation of neuronal progenitor cells. 10 Oxidized low-density lipoprotein (ox-LDL) reduces YBX-1 expression in macrophages, enhances inflammation by promoting mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-κB) pathways, and enhances lipid uptake via contributing to CD36 messenger RNA (mRNA) decay, thereby aggravating atherosclerosis. ...
... 4E-T, a type of eIF4E-binding proteins (4E-BPs), are required to protect the deadenylated target mRNA from degradation by blocking decapping [40]. YBX1, a member of Y-box binding proteins, serves an important role in maternal mRNA degradation, alternative splicing, and transcriptional activity required for early embryonic development [37,38,60]. Consequently, these observed degradation of mRNA-binding proteins during aging could further accelerate mRNAs degradation. ...
... Park and Koh (2020) and Wang et al. (2021) discovered that rotenone treatment significantly enhanced the expression of LC3II and p62 in SH-SY5Y cells. In addition, Heo et al. (2022) found increased levels of ubiquitin and LC3 expression in rotenone-exposed porcine oocytes. These findings aligned with our results. ...
... The intense green fluorescence observed in H 2 O 2 -treated cells confirms the high level of intracellular ROS, while the reduced fluorescence intensity in cells treated with EGCG solutions, emulsions, and digestive solutions indicates their antioxidant effects. These findings are consistent with previous studies that have demonstrated the protective effects of EGCG against oxidative stress in various cell models [42]. ...
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... For real-time reverse transcription-polymerase chain reaction (RT-PCR), we used a previously reported method. 25 Briefly, mRNA from 30 to 50 blastocysts per group was extracted using the DynaBeads mRNA Direct Kit (61012; Thermo Fisher Scientific), according to the manufacturer's instructions. cDNA was obtained via reverse transcription of mRNA using Oligo (dT) 20 primer and SuperScript III Reverse Transcriptase (Thermo Fisher Scientific). ...
... 60 embryos per group (control group and YBX1 knockdown group) were placed in 20 μL of ice-cold 1 × sodium dodecyl sulfate [SDS] sample buffer and incubated at 98°C for 10 min. Based on the previous article (Sui et al., 2020;Sun et al., 2023), the proteins in each sample were separated using 10% SDSpolyacrylamide gel electrophoresis and transferred to a polyvinylidene fluoride membrane (Millipore, Bedford, MA, United States) via electroblotting. The target protein binding site was blocked with Tris-buffered saline containing Tween-20 (TBST) and 5% skim milk powder for 1 h at room temperature. ...
... Approximately 100 embryos in the four-cell stage from each group were collected after 48 h of culture. Western blot analysis was performed as previously reported [33]. Sodium dodecyl sulphate sample buffer (1×) was used to lyse the oocytes at 90 • C for 10 min. ...
... Immunofluorescence staining was performed as described previously. 24 Briefly, the embryos were washed three times with 1% PVA-PBS, fixed in 3.7% paraformaldehyde for 30 min at room temperature, and permeabilized with 1% Triton X-100 in PVA-PBS for 30 min at room temperature. The embryos were then incubated in 3% BSA in PVA-PBS for 1 h at room temperature. ...
