Gennaro Ciliberto's research while affiliated with Istituto Regina Elena - Istituti Fisioterapici Ospitalieri and other places
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Publications (577)
Background: Management of PICC dressing can be performed at home by the patient through adequate training and telenursing. This trial verifies that the incidence of catheter-related complications in home patients, assisted by telenursing, is not greater than that observed in outpatients. Methods: This clinical trial is composed of 72 patients with...
![Toolkit development aims, adapted from Apolone & Brunelli [18]....](publication/381324685/figure/fig1/AS:11431281251440518@1718244978354/Toolkit-development-aims-adapted-from-Apolone-Brunelli-18-PPIPatient-and-Public_Q320.jpg)
Background
Patient and public involvement (PPI) has become an essential part of health research. There is a need for genuine involvement in order to ensure that research is relevant to patients. This can then improve the quality, relevance, and impact of health research, while at the same time reducing wasted research and in doing so bringing scien...
HER2 activating mutations have emerged as oncogenic drivers and therapeutic targets in a variety of human tumors. In breast cancer, these deregulations occur at low frequency, and are mostly detected in HER2 ‐nonamplified, metastatic disease. Preclinical evidence has clarified the role of hotspot mutations in HER2 constitutive activation, defining...
Scientific literature supports the evidence that cancer stem cells (CSCs) retain inside low reactive oxygen species (ROS) levels and are, therefore, less susceptible to cell death, including ferroptosis, a type of cell death dependent on iron-driven lipid peroxidation. A collection of lung adenocarcinoma (LUAD) primary cell lines derived from malig...
Lung NET, classified in typical carcinoids (TC) and atypical carcinoids (AC), are highly heterogeneous in their biology and prognosis. The histological subtype and TNM stage are well-established prognostic factors for lung NET. In a previous work by our group, we demonstrated a significant impact of laterality on lung NET survival outcomes.
We deve...
Glioblastoma, the most common and deadly brain tumor, remains a critical unmet medical need due to the limited effectiveness of current treatments. Drug repurposing has recently emerged as a promising strategy to improve glioblastoma outcomes. Antipsychotic drugs, with their established safety profile and potential to disrupt tumor-neuron interacti...
Therapy of melanoma has improved dramatically over the last years thanks to the development of targeted therapies (MAPKi) and immunotherapies. However, drug resistance continues to limit the efficacy of these therapies. Our research group has provided robust evidence as to the involvement of a set of microRNAs in the development of resistance to ta...
Background
First identified in Drosophila melanogaster, the Hippo pathway is considered a major regulatory cascade controlling tissue homeostasis and organ development. Hippo signaling components include kinases whose activity regulates YAP and TAZ final effectors. In response to upstream stimuli, YAP and TAZ control transcriptional programs involv...
Background
Few data are available about the durability of the response, the induction of neutralizing antibodies, and the cellular response upon the third dose of the anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine in hemato-oncological patients.
Objective
To investigate the antibody and cellular response to the BNT162b2...
DigiONE is a pilot European learning health system in precision oncology that aims to identify optimal cancer treatments by learning from every patient, not just those in trials, through privacy-preserving interrogation of their standardized routine electronic health records
![A TMB distribution [log2(TMB)] among ICI Response groups for different...](publication/377208690/figure/fig2/AS:11431281216205775@1704597712760/A-TMB-distribution-log2TMB-among-ICI-Response-groups-for-different-VAF-filters-B_Q320.jpg)
Background
The current therapeutic algorithm for Advanced Stage Melanoma comprises of alternating lines of Targeted and Immuno-therapy, mostly via Immune-Checkpoint blockade. While Comprehensive Genomic Profiling of solid tumours has been approved as a companion diagnostic, still no approved predictive biomarkers are available for Melanoma aside fr...
The COVID-19 pandemic, once a global crisis, is now largely under control, a testament to the extraordinary global efforts involving vaccination and public health measures. However, the relentless evolution of SARS-CoV-2, leading to the emergence of new variants, continues to underscore the importance of remaining vigilant and adaptable. Monoclonal...
Scientific literature supports the evidence that Cancer Stem Cells (CSCs) retain inside low Reactive Oxygen Species (ROS) levels and are therefore less susceptible to cell death, including ferroptosis, a type of cell death dependent on iron-driven lipid peroxidation. A collection of lung adenocarcinoma (LUAD) primary cell lines derived from maligna...
Introduction
Drug repurposing is a promising strategy to develop new treatments for glioblastoma. In this phase II clinical trial, we evaluated the addition of chlorpromazine to temozolomide in the adjuvant phase of the standard first-line therapeutic protocol in patients with unmethylated MGMT gene promoter.
Methods
This was a multicenter phase I...
Glioblastoma (GBM) is the most frequent and lethal brain tumor, whose therapeutic outcome - only partially effective with current schemes - places this disease among the unmet medical needs, and effective therapeutic approaches are urgently required. In our attempts to identify repositionable drugs in glioblastoma therapy, we identified the neurole...
Background
BRAF-mutant melanoma patients benefit from the combinatorial treatments with BRAF and MEK inhibitors. However, acquired drug resistance strongly limits the efficacy of these targeted therapies in time. Recently, many findings have underscored the involvement of microRNAs as main drivers of drug resistance. In this context, we previously...
The therapeutic scenario of Human Epidermal Growth Factor Receptor 2 positive advanced breast cancer (ABC) has been recently enriched by a number of innovative agents, which are reshaping treatment sequence. While randomized trials have documented an advantage in terms of efficacy, for the newly available agents we lack effectiveness and tolerabili...
KEAP1 mutations have emerged as determinants of survival outcomes in non-small cell lung cancer (NSCLC) patients treated with immune checkpoint inhibitors (ICIs). Given that mutational inactivation of KEAP1 leads to NRF2 transcription, we hypothesized that gene expression can determine immune responsiveness with higher precision. Using the TCGA, we...
Objective
Current prognostic factors for endometrial cancer are not sufficient to predict recurrence in early stages. Treatment choices are based on the prognostic factors included in the risk classes defined by the ESMO-ESGO-ESTRO (European Society for Medical Oncology-European Society of Gynaecological Oncology-European Society for Radiotherapy a...
Background
Molecular Tumor Boards (MTB) operating in real-world have generated limited consensus on good practices for accrual, actionable alteration mapping, and outcome metrics. These topics are addressed herein in 124 MTB patients, all real-world accrued at progression, and lacking approved therapy options.
Methods
Actionable genomic alteration...
Introduction/Background
Endometrial cancer (EC) treatments are related to known prognostic factors included in the risk classes defined by the ESMO-ESGO-ESTRO consensus conference,together with the biomolecular classification. However,these prognostic factors are not sufficient to predict recurrence rate at early stages. The integration of immune s...
Hyperthermic intraperitoneal administration of chemotherapy (HIPEC) increases local drug concentrations and reduces systemic side effects associated with prolonged adjuvant intraperitoneal exposure in patients affected by either peritoneal malignancies or metastatic diseases originating from gastric, colon, kidney, and ovarian primary tumors. Mecha...
The 5th Workshop IRE on Translational Oncology was held in Rome (Italy) on 27–28 March at the IRCCS Regina Elena National Cancer Institute. This meeting entitled “The New World of RNA diagnostics and therapeutics” highlightes the significant progress in the RNA field made over the last years. Research moved from pure discovery towards the developme...
Background
The COVID-19 outbreak had a negative psychological impact on cancer patients. In this study, we analyzed emotional distress and quality of life in patients diagnosed with sarcoma during the first year of the pandemic compared to the previous year.
Methods
We retrospectively enrolled patients with soft tissue, bone sarcoma, and aggressiv...
Background:
In triple negative breast cancer patients treated with neoadjuvant chemotherapy, residual disease at surgery is the most relevant unfavorable prognostic factor. Current guidelines consider the use of adjuvant capecitabine, based on the results of the randomized CREATE-X study, carried out in Asian patients and including a small subset...
The Biomedical Research field is currently advancing to develop Clinical Trials and translational projects based on Real World Evidence. To make this transition feasible, clinical centers need to work toward Data Accessibility and Interoperability. This task is particularly challenging when applied to Genomics, that entered in routinary screening i...
KRAS G12C mutations are found in about 12-13% of LUAD samples and it is unclear whether they are associated with worse survival outcomes in resected, stage I LUAD. We assessed whether KRAS-G12C mutated tumours had worse DFS when compared to KRAS-nonG12C mutated tumours and to KRAS wild-type tumours in a cohort of resected, stage I LUAD (IRE cohort)...
Apoptosis is a form of regulated cell death (RCD) that involves proteases of the caspase family. Pharmacological and genetic strategies that experimentally inhibit or delay apoptosis in mammalian systems have elucidated the key contribution of this process not only to (post-)embryonic development and adult tissue homeostasis, but also to the etiolo...
Introduction:
In breast cancer patients, endocrine therapy may exert a negative impact on sexual functioning in both genders, with potentially relevant consequences concerning quality of life and treatment adherence. The availability of effective interventions to maintain and/or restore sexual health in breast cancer patients is a key issue to a r...
Objectives:
Despite notable advances made in preoperative staging, unexpected nodal metastases after surgery are still significantly detected. Given the promising role of neoadjuvant targeted treatments, the definition of novel predictive factors of nodal metastases is an extremely important issue. In this study we aim to analyze the upstaging rat...
Currently, in clinical practice there are still no useful markers available that are able to diagnose renal cancer in the early stages in the context of population screening. This translates into very high costs for healthcare systems around the world. Analysing urine using an electronic nose (EN) provides volatile organic compounds that can be eas...
Background
This study aimed to characterize the genetic profile of patients with glioma and discuss the impact of next-generation sequencing in glioma diagnosis and treatment.
Methods
Between 2019 and 2022, we analyzed the genetic profile of 99 patients with glioma through the Oncomine Focus Assay. The assay enables the detection of mutations in 5...
Background
Metastases are the major cause of cancer-related morbidity and mortality. By the time cancer cells detach from their primary site to eventually spread to distant sites, they need to acquire the ability to survive in non-adherent conditions and to proliferate within a new microenvironment in spite of stressing conditions that may severely...
Background
Despite the lack of evidence-based on prospective randomized studies, surgery has become the cornerstone of the treatment in patients with pulmonary oligometastatic sarcomas. Our study aimed to construct a composite prognostic score for metachronous oligometastatic sarcoma patients.
Methods
A retrospective analysis was performed on dat...
Precision medicine has driven a major change in the treatment of many forms of cancer. The discovery that each patient is different and each tumor mass has its own characteristics has shifted the focus of basic and clinical research to the singular individual. Liquid biopsy (LB), in this sense, presents new scenarios in personalized medicine throug...
Glioblastoma (GBM) is the most frequent and lethal brain tumor, whose therapeutic outcome - only partially effective with current schemes - places this disease among the unmet medical needs, and effective therapeutic approaches are urgently required. In our attempts to identify repositionable drugs in glioblastoma therapy, we identified chlorpromaz...
Objective
To evaluate the impact of the COVID-19 pandemic on first and follow-up visits for cancer outpatients.
Methods
This is a multicenter retrospective observational study involving three Comprehensive Cancer Care Centers (CCCCs): IFO, including IRE and ISG in Rome, AUSL-IRCCS of Reggio Emilia, and IRCCS Giovanni Paolo II in Bari) and one onco...
Introduction
Immunocompromised patients have been shown to have an impaired immune response to COVID-19 vaccines.
Methods
Here we compared the B-cell, T-cell and neutralizing antibody response to WT and Omicron BA.2 SARS-CoV-2 virus after the fourth dose of mRNA COVID-19 vaccines in patients with hematological malignancies (HM, n=71), solid tumors...
The Hippo pathway plays a critical role for balancing proliferation and differentiation, thus regulating tissue homeostasis. The pathway acts through a kinase cascade whose final effectors are the Yes-associated protein (YAP) and its paralog transcriptional co-activator with PDZ-binding motif (TAZ). In response to a variety of upstream signals, YAP...
Under therapeutic pressure aggressive tumors evolve rapidly. Herein, a luminal B/HER2-low breast cancer was tracked for >3 years during a total of 6 largely unsuccessful therapy lines, from adjuvant to advanced settings. Targeted next generation sequencing (NGS) of the primary lesion, two metastases and 14 blood drawings suggested a striking, unpre...
Background:
The prognostic value of Immunoscore was evaluated in Stage II/III colon cancer (CC) patients, but it remains unclear in Stage I/II, and in early-stage subgroups at risk. An international Society for Immunotherapy of Cancer (SITC) study evaluated the pre-defined consensus Immunoscore in tumors from 1885 AJCC/UICC-TNM Stage I/II CC patie...
BackgroundWHO grade II and III meningiomas are more invasive than grade I malignancies and determine patients’ shorter overall survival. Their tendency to recur after treatment has represented an important therapeutic challenge because of the limited treatment strategies at recurrence. Angiogenesis and mechanistic target of rapamycin (mTOR) activat...
Background
Despite the promise of dual BRAF/MEK inhibition as a therapy for BRAF-mutant (BRAF-mut) melanoma, heterogeneous responses have been observed in patients, thus predictors of benefit from therapy are needed. We have previously identified semaphorin 6A (SEMA6A) as a BRAF-mut-associated protein involved in actin cytoskeleton remodeling. The...
Background:
KEAP1 mutations have been associated with reduced survival in lung adenocarcinoma (LUAD) patients treated with immune checkpoint inhibitors (ICIs), particularly in the presence of STK11/KRAS alterations. We hypothesized that, beyond co-occurring genomic events, clonality prediction may help identify deleterious KEAP1 mutations and thei...
Idiopathic pulmonary fibrosis (IPF) is a disease characterized by progressive scarring of the lung that involves the pulmonary interstitium. The disease may rapidly progress, leading to respiratory failure, and the long-term survival is poor. There are no accurate biomarkers available so far. Our aim was to evaluate the expression of the B4GALT1 in...
The telomeric repeat-binding factor 2 (TRF2) is a telomere-capping protein that plays a key role in the maintenance of telomere structure and function. It is highly expressed in different cancer types, and it contributes to cancer progression. To date, anti-cancer strategies to target TRF2 remain a challenge. Here, we developed a miRNA-based approa...
BRAF-mutated melanoma relapsing after targeted therapies is an aggressive disease with unmet clinical need. Hence the need to identify novel combination therapies able to overcome drug resistance. miRNAs have emerged as orchestrators of non-genetic mechanisms adopted by melanoma cells to challenge therapies. In this context we previously identified...
New evidence on the impact of dysregulation of the CDK4/6 pathway on breast cancer (BC) cell proliferation has led to the development of selective CDK4/6 inhibitors, which have radically changed the management of advanced BC. Despite the improved outcomes obtained by CDK4/6 inhibitors, approximately 10% of tumors show primary resistance, whereas ac...
Immunocompromised patients have been shown to have an impaired immune response to COVID-19 vaccines. Here we compared the B-cell, T-cell and neutralizing antibody response to WT and Omicron BA.2 SARS-CoV-2 virus after the fourth dose of mRNA COVID-19 vaccines in patients with hematological malignancies (HM, n=71), solid tumors (ST, n=39) and immune...
Melanoma is a deadly form of cancer characterized by remarkable therapy resistance. Analyzing the transcriptome of MAPK inhibitor sensitive- and resistant-melanoma, we discovered that APAF-1 is negatively regulated by MITF in resistant tumors. This study identifies the MITF/APAF-1 axis as a molecular driver of MAPK inhibitor resistance. A drug-repo...
Rationale: Metastatic melanoma is the most aggressive and dangerous form of skin cancer. The introduction of immunotherapy with Immune checkpoint Inhibitors (ICI) and of targeted therapy with BRAF and MEK inhibitors for BRAF mutated melanoma, has greatly improved the clinical outcome of these patients. Nevertheless, response to therapy remains high...
Importance
Despite people with impaired immune competence due to an underlying disease or ongoing therapy, hereinafter frail patients, are (likely to be) the first to be vaccinated, they were usually excluded from clinical trials.
Objective
To report adverse reactions of frail patients after receipt of the third dose (booster) administered after c...
Background:
Molecular tumor boards (MTBs) match molecular alterations with targeted anticancer drugs upon failure of the available therapeutic options. Special and local needs are most likely to emerge through the comparative analysis of MTB networks, but these are rarely reported. This manuscript summarizes the state-of-art of 16 active Italian M...
Background
Metastases are the major cause of cancer-related morbidity and mortality. By the time cancer cells detach from their primary site to eventually spread to distant sites, they need to acquire the ability to survive in non-adherent conditions and to proliferate within a new microenvironment in spite of stressing conditions that may severely...
The Hippo pathway and its two key effectors, Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ), are consistently altered in breast cancer. Pivotal regulators of cell metabolism such as the AMP-activated protein kinase (AMPK), Stearoyl-CoA-desaturase 1 (SCD1), and HMG-CoA reductase (HMGCR) are relevant modulat...
Background:
In this study, we evaluated the prognostic value of Immunoscore in patients with stage I-III colon cancer (CC) in the Asian population. These patients were originally included in an international study led by the Society for Immunotherapy of Cancer (SITC) on 2681 patients with AJCC/UICC-TNM stages I-III CC.
Methods:
CD3+ and cytotoxi...
Background
The COVID-19 pandemic led to a rapid reorganization of healthcare activities, leading to reduced access to clinics, interruption of screenings, and treatment schedule modifications in several cancer types. Few data are available on sarcomas. We analyzed COVID-19-related diagnostic delay in a sarcoma referral center in Italy.
Methods
We...
Acute Myeloid Leukaemia (AML) is a haematological malignancy showing a hypervariable landscape of clinical outcomes and phenotypic differences, explainable by heterogeneity at the cellular and molecular level. Among the most common genomic alterations, CBFB-MYH11 rearrangement and FLT3 -ITD gene mutations, have opposite clinical significance and ar...
Simple Summary
The development of oncogene-targeted drugs has radically changed the course of non small cell lung carcinoma (NSCLC) in the advanced stage. Recently, the ADAURA trial demonstrated the efficacy of Osimertinib also in the adjuvant setting of EGFR-mutated NSCLC. This raises the question regarding whether the same paradigm applies also t...
Simple Summary
Although challenging and highly expensive for health systems worldwide, no useful markers are available in clinical practice that aim to anticipate prostate cancer diagnosis in the early stages in the context of wide population screening. Urine analysis via an electronic nose provides volatile organic compounds easily usable in the d...
The outbreak of the coronavirus 2 disease 2019 (COVID-19) puts an enormous burden on healthcare systems worldwide. This may worsen outcomes in patients with severe chronic diseases such as cancer, autoimmune diseases, and immune deficiencies. In this critical situation, only a few available data exist, which do not allow us to provide practical gui...
Background:
Patients with solid or hematological tumors, neurological and immune-inflammatory disorders are potentially fragile subjects at increased risk of experiencing severe COVID-19 and an inadequate response to SARS-CoV-2 vaccination.
Methods:
We designed a prospective Italian multicentrer study to assess humoral and T-cell responses to SA...
Background
Immune checkpoint inhibitors are still unable to provide clinical benefit to the large majority of non-small cell lung cancer (NSCLC) patients. A deeper characterization of the tumor immune microenvironment (TIME) is expected to shed light on the mechanisms of cancer immune evasion and resistance to immunotherapy. Here, we exploited mali...
Background:
The BNT162b2 vaccine conferred 95% protection against COVID-19 in people aged 16 years or older.
Objective:
The aim of this observational study was to evaluate safety and efficacy of vaccine in patients affected by primary brain tumor (PBT).
Methods:
We proposed COVID-19 vaccine to all patients affected by PBT followed by Neuroncol...
Background
Frail patients are considered at relevant risk of complications due to coronavirus disease 2019 (COVID-19) infection and, for this reason, are prioritized candidates for vaccination. As these patients were originally not included in the registration trials, fear related to vaccine adverse events and disease worsening was one of the reaso...
Mutations in the KEAP1 (Kelch-like ECH-associated protein 1)-NRF2 (Nuclear factor erythroid 2-related factor 2) pathway are common in non-small cell lung cancer (NSCLC), albeit with a prevalence of KEAP1 mutations in lung adenocarcinoma (LUAD) and an equal representation of KEAP1 and NFE2L2 (the gene encoding for NRF2) alterations in lung squamous...
Immune checkpoint inhibitors (ICI) based on anti-CTLA-4 (αCTLA-4) and anti-PD1 (αPD1) are being tested in combination with different therapeutic approaches including other immunotherapies such as neoantigen cancer vaccines (NCV). Here we explored, in two cancer murine models, different therapeutic combinations of ICI with personalized DNA vaccines...
The Telomeric Repeat binding Factor 2 (TRF2), a key protein involved in telomere integrity, is over-expressed in several human cancers and promotes tumor formation and progression. Recently, TRF2 has been also found outside telomeres where it can affect gene expression. Here we provide evidence that TRF2 is able to modulate the expression of microR...
Background:
Triple negative breast cancer (TNBC) is a heterogeneous group of tumors with early relapse, poor overall survival, and lack of effective treatments. Hence, new prognostic biomarkers and therapeutic targets are needed.
Methods:
The expression profile of all twenty-five human selenoproteins was analyzed in TNBC by a systematic approach...
Purpose
The major improvements in the diagnosis and treatment of colorectal cancer (CRC) over the past decades increased the patients’ survival rates. Despite this, patients and clinicians still need to address the long-term physical and psychosocial effects over time. This paper aims to prospectively assess CRC patients’ HR-QoL psychological distr...
Background
Frail patients are considered at relevant risk of complications due to COVID-19 infection and, for this reason, are prioritized candidates for vaccination. As these patients were originally not included in the registration trials, fear related to vaccine side-effects and disease worsening was one of the reasons for vaccine hesitancy. Her...
Objectives
Despite extensive efforts to monitor the diffusion of COVID-19, the actual wave of infection is worldwide characterized by the presence of emerging SARS-CoV-2 variants. The present study aims to describe the presence of yet undiscovered SARS-CoV-2 variants in Italy.
Methods
Next Generation Sequencing was performed on 16 respiratory samp...
Background: Patients with solid or hematological tumors, neurological and immune-inflammatory disorders represent potentially fragile subjects with increased risk to experience severe COVID-19 and inadequate response to SARS-CoV2 vaccination.
Methods: We designed a prospective Italian multicentric study to assess humoral and T-cell response to SARS...
In the last decades cancer immunotherapy has revolutionized the field of cancer research, dramatically changing the standard clinical practice in cancer treatment. Indeed, the different immunotherapy approaches, including immune checkpoint blockade, adoptive chimeric antigen receptor T-cell therapy, oncolytic viruses, and therapeutic cancer vaccine...
Background
Autophagy is an intracellular degradation system that removes unnecessary or dysfunctional components and recycles them for other cellular functions. Over the years, a mutual regulation between lipid metabolism and autophagy has been uncovered.
Methods
This is a narrative review discussing the connection between SCD1 and the autophagic...
Background
The evolution of therapeutic landscape of human epidermal growth factor receptor-2 (HER2)-positive breast cancer (BC) has led to an unprecedented outcome improvement, even if the optimal sequence strategy is still debated. To address this issue and to provide a picture of the advancement of anti-HER2 treatments, we performed a large, mul...
Background: Despite the promise of dual BRAF/MEK inhibition as a therapy for BRAF-mutant (BRAF-mut) melanoma, heterogeneous responses have been observed in patients, thus predictors of benefit from therapy are needed. We have previously identified semaphorin 6A (SEMA6A) as a BRAF-mut-associated protein involved in actin cytoskeleton remodeling. The...
Background:
We present immunogenicity data 6 months after the first dose of BNT162b2 in correlation with age, gender, BMI, comorbidities and previous SARS-CoV-2 infection.
Methods:
An immunogenicity evaluation was carried out among health care workers (HCW) vaccinated at the Istituti Fisioterapici Ospitalieri (IFO). All HCW were asked to be vacc...
Clinical outcomes of COVID-19 patients are worsened by the presence of co-morbidities, especially cancer leading to elevated mortality rates. SARS-CoV-2 infection is known to alter immune system homeostasis. Whether cancer patients developing COVID-19 present alterations of immune functions which might contribute to worse outcomes have so far been...
To date, studies on anti-severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) vaccine efficacy in blood cancers show that different responses may be observed according to the haematological malignancy diagnosis, stage of disease and ongoing treatment. Immune responses are often lower compared to healthy controls, second doses appear to be c...
The COVID-19 pandemic caused by SARS-CoV-2 has made the development of safe and effective vaccines a critical priority. To date, four vaccines have been approved by European and American authorities for preventing COVID-19 but the development of additional vaccine platforms with improved supply and logistics profiles remains a pressing need. Here w...
A convalescent, non-severe, patient with COVID-19 was enrolled as a hyper-immune plasma voluntary donor by the Immuno-Hematology and Transfusion Unit of the Regina Elena National Cancer Institute in Rome, under the TSUNAMI national study criteria. During a nearly 6-month period (May–October 2020), the patient was closely monitored and underwent fou...
Aims
The minimally invasive procedures used in the diagnostic workup of patients with advanced non-small cell lung cancer (NSCLC) often provide poor yields of pathological material suitable for molecular analyses. Not infrequently, the DNA yield from small biopsies/cytological samples is insufficient for the assessment of genomic biomarkers that in...
The actual role of chemotherapy in vulvar cancer is undeniably a niche topic. The low incidence of the disease limits the feasibility of randomized trials. Decision making is thus oriented by clinical and pathological features, whose relevance is generally weighted against evidence from observational studies and clinical practice. The therapeutic m...
Patients diagnosed with malignancy, neurological and immunological disorders, i.e., fragile patients, have been excluded from COVID-19 vaccine trials. However, this population may present immune response abnormalities, and relative reduced vaccine responsiveness. Here we review the limited current evidence on the immune responses to vaccination of...
Citations
... In fact, although the data demonstrate that this panel was able to identify mutations in almost all advanced melanoma, in 10% of these, no alterations were identified. In these samples, it might be worth investigating other markers, such as using a panel for fusion genes (e.g., ALK fusions) [28] or a Comprehensive Genomic Profiling (CGP) panel [29,30], allowing the identification of mutations in uncommonly altered genes. With our panel, the primary and metastatic samples showed the same molecular structure; however, the extension to other markers could also be performed to understand whether there are any acquired variants in the metastases that are not present in the primary lesion. ...
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... The Pfizer-BioNTech and Moderna mRNA vaccines, among others, have received global approval, reflecting ongoing efforts to combat the evolving virus [163,166,167]. Several vaccines have been approved in different parts of the world, such as CoronaVac, BBIBP-CorV, CoviVac, Covaxin, Oxford-AstraZeneca vaccine (ChAdOx1 nCoV-19), Sputnik V, the Johnson & Johnson vaccine, Convidicea, RBD-Dimer, and EpiVacCorona [168,169]. ...
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... Based on the discovery of stimulatory synaptic connections between neurons and GBM mediated by monoamines and their receptors (34,35), and on our previous work demonstrating the direct antitumor potential of CPZ and its synergistic effects with TMZ in restraining GBM growth in vitro (36), we carried out a Phase II multicenter clinical trial to evaluate the e cacy of CPZ in combination with TMZ in the rst-line treatment of GBM patients with an unmethylated MGMT gene promoter, a group known to exhibit higher resistance to TMZ (37). The present study delves into a novel mechanism underlying CPZ's anti-neoplastic properties, focusing on its potential role in circumventing TMZ resistance by hindering MGMT-independent DNA repair pathways. ...
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... Thanks to this approach, we demonstrated the ability of LNPs-miRs to potentiate targeted therapies in vivo and to delay the emergence of drug resistance by inhibiting core escape pathways of resistance [11]. Regarding miR-4443 and miR-4488, we have recently discovered that these oncomiRs are main orchestrators for the enhanced migratory and invasive phenotypes, that are a hallmark of drug resistant melanoma cells [12]. ...
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... Dataset overview[27]. ...
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... A recent meta-analysis by Marinelli et al. [35] reviewed published neoadjuvant and preoperative chemo-immunotherapy trials, demonstrating an event-free survival (EFS) advantage in PD-L1 negative tumors. The study showed improved EFS in the experimental arm (hazard ratio, HR 0.55), irrespective of stage, histology, or PD-L1 expression (PD-L1 negative, HR 0.74). ...
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... Indeed, in the real-world clinical practice, NGS panels are being increasingly requested by oncologists, although the utility of upfront full molecular profiling in all cancer patients is still a matter of dispute [25]. Selection criteria across different MTB are not standardized and specific guidelines are not available, although real-world MTB experiences as well as position papers from scientific societies addressing this issue are increasingly being published [26][27][28][29]. ...
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... One such example of successful commercialization is found in the RNA-based early-cancer diagnostic field, namely GASTROClear DX [80]. GASTROClear DX is a miRNA-based assay that serves as the world's first molecular blood test for early detection of gastric cancer (GC) within susceptible populations [80,81]. The release of GASTROClear DX in 2020 allowed for the risk assessment of GC in seemingly healthy asymptomatic people while simultaneously provided a notable accuracy of 87% higher than any other conventionally quantified blood biomarker for GC such as serum pepsinogen, CEA, and CA19-9 tumor markers [80,81]. ...
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... In early-stage NSCLC, however, while several studies have shown that KRAS mutations negatively influence the prognosis (15)(16)(17), others have shown no significant effect (18)(19)(20). Most recently, it was reported that KRAS G12C mutation (but not other KRAS mutations or with no mutation in KRAS) significantly increased risk of disease recurrence in stage I surgically resected lung adenocarcinomas (21). However, while the study found this in two distinct local cohorts of IRE-LUAD (Rome, Italy) and MSK-LUAD (New York, USA), data extracted from The Cancer Genome Atlas (TCGA) showed no significant difference. ...
... Its downregulation may thus represent a protective, anti-inflammatory response post-ICH [62,63]. Additionally, the observed downregulation in apoptosis pathways might reflect a cellular survival strategy aimed at limiting cell death in the acute phase of hemorrhagic injury [64][65][66]. The severity of ICH appears to further influence downregulated pathways, with severe ICH showing reduced expression in pathways critical for neuronal communication, like axon guidance and glutamatergic synapse. ...
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