May 2024
Ecotoxicology and Environmental Safety
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May 2024
Ecotoxicology and Environmental Safety
May 2024
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6 Reads
Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy
August 2023
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11 Reads
Translational Cancer Research
Background Prostate cancer (PCa) remains a major prevalent cancer worldwide and has a poor prognosis. The sex-determining region Y (SRY)-related high-mobility group (HMG) box (SOX) family is a series of transcription factors (TFs) involved in regulating many biological processes (BPs). In tumors, however, SOX genes are frequently deregulated. Tumorigenic deregulation took place at the transcriptional, translational, and posttranslational levels. This leads them to be correlated to tumor progression and poor clinical outcomes in PCa. Nevertheless, the SOX family prognostic role in PCa still needs further investigation. Methods A SOX family-related prognostic signature was developed by performing LASSO (Least absolute shrinkage and selection operator) Cox regression analysis. The construction of a lncRNA-miRNA-mRNA regulatory axis for PCa was performed using a ceRNA network. Results Upregulation was observed in the expression of SOX4/8/11/12/14, while downregulation was observed for SOX2/5/7/13/15/30 in PCa. Consensus clustering identified four clusters of PCa patients based on these differentially expressed SOX family members. The constructed SOX family-related prognostic signature, which includes five SOX family members (SOX5/8/11/12/30), performed well in predicting PCa-patient prognosis. B cells, CD4+ T cells, CD8+ T cells, neutrophils, macrophages, and dendritic cell immune infiltration levels had a significant association with PCa-patient risk scores. Based on additional analysis, a significant association was also suggested between SOX family expression and tumor mutational burden (TMB), microsatellite instability (MSI), and drug sensitivity. By constructing a ceRNA network, a lncRNA SGMS1-AS1/miR-194-5p/SOX5 regulatory axis was developed for PCa. Conclusions Herein, a SOX family-related prognostic signature was identified and was found to perform well in predicting PCa-patient prognosis. A lncRNA SGMS1-AS1/miR-194-5p/SOX5 regulatory axis was also identified for PCa progression.
July 2023
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4 Reads
Background There is currently no robust prognostic model for sarcomatous renal cell carcinoma (sRCC), which could help physicians make better decisions. Objectives To build an accurate predictive model for patients who have sRCC by investigating the important characteristics that influence the overall survival of patients. Design and Methods The Surveillance, Epidemiology and Results (SEER) database of the U.S. National Cancer Institute was used for gathering the dataset of sRCC patients. Following data preprocessing, the data was separated into the training set and the test set in an 8:2 ratio. Mann-Whitney U test and Chi-square test were used to verify whether the data set was evenly divided. Univariate Cox proportional hazard model, Kaplan-Meier analysis and machine learning (ML) algorithm were employed to identify the risk features on overall survival (OS). 10 reliable features were selected to construct six ML models. Model performance, predictive accuracy, and clinical benefits were evaluated by the receiver operating characteristic curves (ROC), calibration plots, and decision curve analysis (DCA) respectively. Results After data preprocessing, 692 patients with sRCC from 1975 to 2019 were included in this study. Ten variables including stage group, T stage, M stage, age, surgery, N stage, tumor size, chemotherapy, histological grade, and radiotherapy were selected as reliable features for machine learning model training. All the models show good prediction performance, among which XGBoost has the best prediction accuracy and stability. The DCA showed that all models except Adaboost could be used to support clinical decision-making with the 90-day, 1-, 2-, 3- and 5-year OS model. Conclusions Six machine learning models were developed to predict 90-day, 1-, 2-, 3- and 5-year overall survival in patients with sRCC. Model evaluations showed that the XGBoost model had the best predictive accuracy and clinical net benefit. These models can help make treatment decisions for patients with sRCC.
June 2023
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7 Reads
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1 Citation
Psychiatry Research
Patient Health Questionnaire-9 (PHQ-9) is the most widely used tool for screening for major depressive disorder (MDD). Although its reliability and validity have been proven, missed or misjudged cases during MDD screening are often encountered. A nomogram that considers the weights of depressive symptoms was developed using data from premature ejaculation patients to improve screening accuracy. During a 33-month prospective study, a training cohort comprising 605 participants from Xijing Hospital was used to develop and internally validate the nomogram. A validation cohort comprising 461 patients from Xi'an Daxing Hospital was also used to externally test the nomogram. The nomogram was established by integrating the LASSO regression-based optimal predictors of MDD according to their coefficients in a multivariate logistic regression model. The nomogram was well-calibrated during internal and external validations. Moreover, it showed a better discriminatory capacity and yielded more net benefits in both validations than PHQ-9. With better performance, the nomogram may help reduce the number of missed or misjudged cases during MDD screening. This study is the first to weigh the direct indicators of MDD under the DSM-5 criteria, presenting a fresh concept that can be applied to other populations to enhance screening accuracy.
April 2023
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223 Reads
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4 Citations
BMC Urology
Background Few studies have compared the use of transabdominal ultrasound (TAUS) and magnetic resonance imaging (MRI) to measure prostate volume (PV). In this study, we evaluate the accuracy and reliability of PV measured by TAUS and MRI. Methods A total of 106 patients who underwent TAUS and MRI prior to radical prostatectomy were retrospectively analyzed. The TAUS-based and MRI-based PV were calculated using the ellipsoid formula. The specimen volume measured by the water-displacement method was used as a reference standard. Correlation analysis and intraclass correlation coefficients (ICC) were performed to compare different measurement methods and Bland Altman plots were drawn to assess the agreement. Results There was a high degree of correlation and agreement between the specimen volume and PV measured with TAUS (r = 0.838, p < 0.01; ICC = 0.83) and MRI (r = 0.914, p < 0.01; ICC = 0.90). TAUS overestimated specimen volume by 2.4ml, but the difference was independent of specimen volume (p = 0.19). MRI underestimated specimen volume by 1.7ml, the direction and magnitude of the difference varied with specimen volume (p < 0.01). The percentage error of PV measured by TAUS and MRI was within ± 20% in 65/106(61%) and 87/106(82%), respectively. In patients with PV greater than 50 ml, MRI volume still correlated strongly with specimen volume (r = 0.837, p < 0.01), while TAUS volume showed only moderate correlation with specimen (r = 0.665, p < 0.01) or MRI volume (r = 0.678, p < 0.01). Conclusions This study demonstrated that PV measured by MRI and TAUS is highly correlated and reliable with the specimen volume. MRI might be a more appropriate choice for measuring the large prostate.
April 2023
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11 Reads
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1 Citation
Aging
Cerebral ischemia/reperfusion (I/R) is a common neurological disease. Homeobox A11 antisense RNA (HOXA11-AS), a long non-coding RNA (lncRNA), has been demonstrated as an important regulator in diverse human cancers. However, its function and regulatory mechanism in ischemic stroke remains largely unknown. Dexmedetomidine (Dex) have received wide attraction because of its neuroprotective effects. This study aimed to explore the possible link between Dex and HOXA11-AS in protecting neuronal cells from by ischemia/reperfusion-induced apoptosis. We used oxygen-glucose deprivation and reoxygenation (OGD/R) in mouse neuroblastoma Neuro-2a cells and middle cerebral artery occlusion (MACO) mouse model to test the link. We found that Dex significantly alleviated OGD/R-induced DNA fragmentation, cell viability and apoptosis, and rescued the decreased HOXA11-AS expression after ischemic damage in Neuro-2a cells. Gain-/loss-of-function studies revealed that HOXA11-AS promoted proliferation, inhibited apoptosis in Neuro-2a cells exposed to OGD/R. Knockdown of HOXA11-AS decreased the protective effect of Dex on OGD/R cells. HOXA11-AS was found to transcriptionally regulate microRNA-337-3p (miR-337-3p) expression as evidenced by luciferase reporter assay, while miR-337-3p expression was upregulated following ischemia in vitro and in vivo. Besides, knockdown of miR-337-3p protected OGD/R-induced apoptotic death of Neuro-2a cells. Furthermore, HOXA11-AS functioned as a competing endogenous RNA (ceRNA) and competed with Y box protein 1 (Ybx1) mRNA for directly binding to miR-337-3p, which protected ischemic neuronal death. Dex treatment protected against ischemic damage and improved overall neurological functions in vivo. Our data suggest a novel mechanism of Dex neuroprotection for ischemic stroke through regulating lncRNA HOXA11-AS by targeting the miR-337-3p/Ybx1 signaling pathway, which might help develop new strategies for the therapeutic interventions in cerebral ischemic stroke.
March 2023
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22 Reads
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11 Citations
BMC Cancer
Background Prostate cancer (PCa), one of the common malignant tumors, is the second leading cause of cancer-related deaths in men. The circadian rhythm plays a critical role in disease. Circadian disturbances are often found in patients with tumors and enable to promote tumor development and accelerate its progression. Accumulating evidence suggests that the core clock gene NPAS2 (neuronal PAS domain-containing protein 2) has been implicated in tumors initiation and progression. However, there are few studies on the association between NPAS2 and prostate cancer. The purpose of this paper is to investigate the impact of NPAS2 on cell growth and glucose metabolism in prostate cancer. Methods Quantitative real-time PCR (qRT-PCR), immunohistochemical (IHC) staining, western blot, GEO (Gene Expression Omnibus) and CCLE (Cancer Cell Line Encyclopedia) databases were used to analyze the expression of NPAS2 in human PCa tissues and various PCa cell lines. Cell proliferation was assessed using MTS, clonogenic assays, apoptotic analyses, and subcutaneous tumor formation experiments in nude mice. Glucose uptake, lactate production, cellular oxygen consumption rate and medium pH were measured to examine the effect of NPAS2 on glucose metabolism. The relation of NPAS2 and glycolytic genes was analyzed based on TCGA (The Cancer Genome Atlas) database. Results Our data showed that NPAS2 expression in prostate cancer patient tissue was elevated compared with that in normal prostate tissue. NPAS2 knockdown inhibited cell proliferation and promoted cell apoptosis in vitro and suppressed tumor growth in a nude mouse model in vivo. NPAS2 knockdown led to glucose uptake and lactate production diminished, oxygen consumption rate and pH elevated. NPAS2 increased HIF-1A (hypoxia-inducible factor-1A) expression, leading to enhanced glycolytic metabolism. There was a positive correlation with the expression of NPAS2 and glycolytic genes, these genes were upregulated with overexpression of NPAS2 while knockdown of NPAS2 led to a lower level. Conclusion NPAS2 is upregulated in prostate cancer and promotes cell survival by promoting glycolysis and inhibiting oxidative phosphorylation in PCa cells.
February 2023
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15 Reads
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1 Citation
Medicine
Rationale: Wounds caused by firearms are intractable problems in treating war traumas and clinical management. Conventional open surgery inflicts large injury and leads to slow recovery. At the same time, most patients suffer from compound injuries with the critical condition and poor operation tolerance. Thus, it is crucial to probe into the minimally invasive surgical removal of residual kidney bullets. Patient concerns: We report a case where a bullet remained in the right renal parenchyma on the patient, with penetrating injury in his liver. Diagnosis: Obviously the patient has suffered gunshot wound with a bullet stuck in his kidney, while his liver function was impacted. Interventions: Six months after the injury, we performed the minimally-invasive procedures on the patient with percutaneous nephroscope technology and laser technology under the guidance of ultrasound localization. The bullet and ammunition granulation and scar surrounding tissue were fully removed. Intraoperative bleeding was little, while the incision was small. The patient could leave the bed and walk on the 1st postoperative day. The drainage tube was removed on the 3rd postoperative day, after which the patient was discharged on the 4th postoperative day. Outcomes: The patient recovered well after surgery and was followed up for 5 years. The latest examination of his liver and kidney function was as follows: alanine aminotransferase 61IU/L, aspartate aminotransferase 33 IU/L, albumin/globulin 46.6/26.0, total bilirubin 19.1μmol/L, direct bilirubin 4.9μmol/L, indirect bilirubin 14.2μmol/L, alkaline phosphatase 111 IU/L, creatinine 57μmol/L, urea 5.16mmol/L, cystatin 0.73mg/L. The plain computed tomography scan showed a few calcifications in the liver and a patchy low-density shadow in the right kidney. It was proved that the liver and kidney function of the patient recovered well, and his living qualify has come back to the track, with no postoperative complications. Lessons: Innovative integration of percutaneous nephroscopy technology and laser was used to remove kidney foreign bodies and developed the optimal surgical plan, small trauma, fast recovery, and the treatment of kidney foreign bodies was newly explored.
November 2022
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10 Reads
Background: Prostate cancer (PCa), one of the common malignant tumors, is the second leading cause of cancer-related deaths in men. The circadian rhythm plays a critical role in disease. Circadian disturbances are often found in patients with tumors and enables to promotes tumor development and accelerate its progression. Accumulating evidence suggests that the core clock gene NPAS2 (neuronal PAS domain-containing protein 2) has been implicated in tumors initiation and progression. However, there are few studies on the association between NPAS2 and prostate cancer. The purpose of this paper is to investigate the impact of NPAS2 on cell growth and glucose metabolism in prostate cancer. Methods: Quantitative real-time PCR (qRT-PCR), immunohistochemical (IHC) staining, western blot, and CCLE (Cancer Cell Line Encyclopediadatabase) were used to analyze the expression of NPAS2 in human PCa tissues and various PCa cell lines. Cell proliferation was assessed using MTS, clonogenic assays, apoptotic analyses, and subcutaneous tumor formation experiments in nude mice. Glucose uptake, lactate production, cellular oxygen consumption rate and medium pH were measured to examine the effect of NPAS2 on glucose metabolism. The relation of NPAS2 and glycolytic genes was analyzed based on TCGA (The Cancer Genome Atlas) database. Results : Our data showed that NPAS2 expression in prostate cancer patient tissue is elevated compared with that in normal prostate tissue. NPAS2 knockdown inhibited cell proliferation and promoted cell apoptosis in vitro and suppressed tumor growth in a nude mouse model in vivo. NPAS2 knockdown led to glucose uptake and lactate production diminished, oxygen consumption rate and pH elevated. NPAS2 increased HIF-1A (hypoxia-inducible factor-1A) expression, leading to enhanced glycolytic metabolism. There was a positive correlation with the expression of NPAS2 and glycolytic genes, these genes were upregulated with overexpression of NPAS2 while knockdown of NPAS2 led to a lower level. Conclusion: NPAS2 is upregulated in prostate cancer and promotes cell survival by promoting glycolysis and inhibiting oxidative phosphorylation in PCa cells.
... Similar to ejaculation function [129], erectile function is closely related to mental and psychological health [130] (Figure 1). Older men tend to suffer from depression, with a prevalence of around 30% [131], and drugs used to treat depression may significantly affect erectile function [132] (Table 1). ...
June 2023
Psychiatry Research
... Recent studies have proposed TAUS as a preferable examination method over transrectal ultrasound (TRUS) due to its widespread availability and enhanced patient comfort. Furthermore, TAUS has demonstrated comparable accuracy to magnetic resonance imaging (MRI) examinations [12]. Additionally, TAUS and TRUS have demonstrated similar accuracy in detecting enlarged prostate volume, making TAUS a valuable alternative to TRUS in clinical practice [13]. ...
April 2023
BMC Urology
... NPAS2 has been found to be upregulated in multiple cancers and to play a role in promoting cell survival by modulating the Warburg effect. 80,81 Future studies could focus on evaluating whether bovine BM-MSCs have a higher potential to support tumor formation 82 and/or if they co-opt cancer mechanisms to maintain their state of potency. One potential limitation of this work is that the bovine MSCs were cultured differently than the murine and human MSCs (Table 1), and MSCs were derived from individuals of different sex across species, which may influence the findings of the scRNA-seq. ...
March 2023
BMC Cancer
... Using both these modalities together helped us avoid damaging the kidney or its blood vessels, which is crucial for controlling bleeding and having a clear view during surgery. Wang B et al used CT with 3D reconstruction and ultrasound guidance for the same purpose (7). Jhaveri H et al (4) used percutaneous ultrasound lithotripsy to fragment stones over bullet, we used pneumatic modality of lithotripsy for the same purpose. ...
February 2023
Medicine
... Wang et al (32) investigated the mechanisms underlying the role of miR-1236-3p in RCC progression and found that miR-1236-3p directly targets the P21 promoter to regulate its gene expression by using a ChIP assay with biotinylated miR-1236-3p, with the ultimate effect to inhibit its function and leading to the suppression of RCC cell proliferation. The result of a luciferase reporter assay showed that miR-24-1 overexpression enhanced fructose-1,6-bisphosphatase transcription by increasing its enhancer activity to attenuate RCC proliferation and metastasis (33). ...
August 2022
... Several recent research also reported that long-term ozone exposure has a substantial effect on hypertension, blood pressure, and Alzheimer disease. For example, Niu et al. found that per 10 μg/m 3 rise in ozone concentration was associated with a 13.70% (95% CI 4.80, 23.3) increase in the incidence of hypertension based on a national crosssectional investigation in China [72]. Jung et al. reported an increased risk of 2.11 (95% CI: 2.92, 3.33) of Alzheimer's disease with per increase of 10.91 ppb in ozone using the data from the Taiwan Environmental Protection Agency during 2000-2010 [73]. ...
September 2022
Ecotoxicology and Environmental Safety
... 4 Male sexual function can be assessed by several psychometric scales: the 6-item International Index for Erectile Function (IIEF-6), the Erection Hardness Scale (EHS), and the Masturbation Erection Index (MEI) can be used to assess aspects related to erectile function; moreover, the Premature Ejaculation Diagnostic Tool (PEDT) can be used to assess the presence of premature ejaculation (PE) and its clinical consequences. [5][6][7][8][9][10] Several studies have confirmed the diagnostic accuracy of the EHS and IIEF-6. [11][12][13][14][15] The MEI is an adaptation of the IIEF-6 that is applied to masturbation; this scale is particularly useful for erectile dysfunction (ED) patients who have not engaged in penetrative sexual intercourse in the past 4 weeks. ...
June 2022
... However, the scientific understanding in this area is not yet comprehensive, and well-documented studies indicate that even low-intensity electromagnetic radiation can influence biochemical processes and produce measurable physiological effects [11][12][13][14][15][16][17][18][19]. This situation has gradually raised awareness and concerns among the general population regarding their continuous exposure to RF fields. ...
June 2022
... To this end, premature ejaculation (PE) stands out as highly prevalent male sexual dysfunctions, causing a consistent degree of depressive and anxious symptomatologys. 1 Numerous epidemiological studies indicate that the prevalence of PE hovers around 20%−30%, 2 a comparable study conducted in China reported a prevalence rate of PE ranging between 7% and 11%. 3 anxiety, frustration, and/or avoidance of intimate sexual encounters. 4,5 Pharmacotherapy remains the prevailing treatment modality for PE. ...
May 2022
European Journal of Clinical Investigation
... Since then, several case series of robotic-assisted nephrectomies with cavotomies or IVCTT for different thrombus levels have been described. One of the earliest was described by Ma et al. in 2021, where 20 patients with thrombus levels ranging from 0 to III received RARN/IVCTT [18]. A recent study found significantly improved outcomes with robotic assistance in terms of operative time, blood loss, and volume of blood transfusion [19]. ...
August 2021
Medicine