Eva Wagner's research while affiliated with Ruhr-Universität Bochum and other places

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Publications (1)

Accumulation of Mo-nitrogenase proteins and nifK-lacZ expression. R. capsulatus strains were grown in RCV minimal medium with the indicated molybdate concentrations. The strains used were as follows: B10S, the wild-type strain (A and C); R438, the ΔmodABC mutant (B and D); B10S::pYP348, the wild-type strain carrying nifK-lacZ (E); and R438::pYP348, the ΔmodABC mutant carrying nifK-lacZ (F). (A to D) Western analyses were performed using antisera against NifH (A and B) or NifDK (C and D). “NifH*” marks the ADP-ribosylated form of NifH. Molecular weight data are given in thousands. (E and F) LacZ (β-galactosidase) activity is given in Miller units (MU) (23). The results represent the means and standard deviations of the results of at least three independent measurements.
Expression of mop-lacZ and Mop-FLAG accumulation. R. capsulatus strains were grown in RCV minimal medium with the indicated molybdate concentrations. (A and B) The strains used were B10S::pEW18, the wild-type strain carrying mop-lacZ (A), and B10S::pEW10, the wild-type strain carrying mop-FLAG (B). (A) LacZ (β-galactosidase) activity is given in Miller units (23). The results represent the means and standard deviations of the results of at least three independent measurements. (B) Western analysis was performed using FLAG antibodies. Molecular weight data are given in thousands.
Expression of iscN-FLAG, iscN-lacZ, nifU1-FLAG, and nifU1-lacZ reporter fusions. R. capsulatus strains were grown in RCV minimal medium with the indicated molybdate concentrations. The strains used were as follows: B10S::pEW13, the wild-type strain carrying iscN-FLAG (A); B10S::pEW58, the wild-type strain carrying iscN-lacZ (B); B10S::pEW51, the wild-type strain carrying nifU1-FLAG (C); and B10S::pEW53, the wild-type strain carrying nifU1-lacZ (D). (A and C) Western analysis was performed using FLAG antibodies. Molecular weight data are given in thousands. (B and D) LacZ (β-galactosidase) activity is given in Miller units (23). The results represent the means and standard deviations of the results of at least three independent measurements.
Diazotrophic growth of iscN mutant strains. (A and B) To examine diazotrophic growth, R. capsulatus strains were grown in RCV minimal medium under a pure N2 atmosphere. The strains used in the experiments represented by panel A were B10S (wild type; open circles) and YP340 (ΔiscN mutant; filled circles). The strains used in the experiments represented by panel B were BS85 (ΔnifDK mutant; open squares) and BS85-YP340 (ΔnifDK-ΔiscN mutant; filled squares). Growth experiments were done three times, and one representative data set is shown. In addition, the ΔnifDK and ΔnifDK-ΔiscN strains were grown in RCV medium containing serine. (C) Samples taken at the logarithmic phase (Log phase) and the stationary phase (Stat phase) were examined by Western analysis. Antibodies raised against R. capsulatus NifDK were used to detect AnfD and AnfK proteins. Molecular weight data are given in thousands.
Proteome Profiling of the Rhodobacter capsulatus Molybdenum Response Reveals a Role of IscN in Nitrogen Fixation by Fe-Nitrogenase
  • Article
  • Full-text available

January 2016

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52 Reads

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19 Citations

Journal of Bacteriology

Journal of Bacteriology

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Eva Wagner

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Bernd Masepohl

Importance: Biological nitrogen fixation is a central process in the global nitrogen cycle by which the abundant but chemically inert dinitrogen (N2) is reduced to ammonia (NH3), a bioavailable form of nitrogen. Nitrogen reduction is catalyzed by nitrogenases found in diazotrophic bacteria and archaea, but not in eukaryotes. All diazotrophs synthesize molybdenum-dependent nitrogenases. In addition, some diazotrophs including Rhodobacter capsulatus possess catalytically less efficient alternative Mo-free nitrogenases, whose expression is Mo-repressed. Despite the importance of Mo in biological nitrogen fixation, this is the first study analyzing the proteome-wide Mo response in a diazotroph. IscN was recognized as a novel member of the molybdoproteome in R. capsulatus. It was dispensable for Mo-nitrogenase activity but supported diazotrophic growth under Mo-limiting conditions.

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Citations (1)

... Therefore, a RNA molecules can be easily degraded and the integrity of RNA molecules is quite important for subsequent real-time PCR experiment (which reflects the gene expression levels at the moment of sampling), it requires to take a lot of time and effort to reduce the RNA degradation during the preparation of RNA samples [8]. The lacZ gene encoding βgalactosidase is another reporter gene that is commonly used in photosynthetic bacteria [9][10][11]. A colorimetric assay is used to determine the activity of β-galactosidase, which is responsible for the degradation of β-galactosyl linkages [12,13]. ...

Reference:

A Red Fluorescent Protein Reporter System Developed for Measuring Gene Expression in Photosynthetic Bacteria under Anaerobic Conditions
Proteome Profiling of the Rhodobacter capsulatus Molybdenum Response Reveals a Role of IscN in Nitrogen Fixation by Fe-Nitrogenase
Journal of Bacteriology

Journal of Bacteriology

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Eva Wagner

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[...]

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Bernd Masepohl