Eva A. Buckner's research while affiliated with University of Florida and other places

... Culex coronator has a wide distribution, extending from Argentina to the United States (Wilkerson et al. 2021), and can be found over a large range of breeding sites: stagnant or slow-moving water in ground pools and seeps, ditches, culverts, artificial containers, ground depressions, tire ruts, and even dredge sites, most commonly in open, sunlit aquatic habitats (Schluep et al. 2023). In this study, Cx. coronator larvae were collected in a ground puddle, with no other mosquito larvae present. ...

... Chen et al. 8 highlight a simple, tractable, high-throughput DNA extraction protocol of mosquito samples for whole genome sequencing, utilizing magnetic DNA-binding beads. ...

... As highly mobile generalist consumers, insectivorous bats contribute to stabilizing and connecting local food webs in their ecosystem 8,9 . Additionally, they provide important ecosystem services by suppressing agricultural pests [10][11][12][13] , forest pests 4,14 , and vectors of parasites of humans 15,16 and livestock 17,18 . For example, based on DNA metabarcoding of guano collected from roosts, Maslo et al. 13 found that bats consumed ≥ 160 known agricultural pest species or disease vectors. ...

... In addition, recent studies have identified four SNPs, V410L, S723T, D1763Y, and Q1853R, within the vgsc in Ae. aegypti (Haddi et al. 2017, Chung et al. 2019, Saavedra-Rodriguez et al. 2019. A recent study documented the occurrence of these four SNPs and two novel SNPs, F174I and E478K within Ae. aegypti from Florida for the first time in 2021 (Kosinski et al. 2022). ...

... A previous study from their group [7] made the distinction between a vertically transmitted core virome and an environmentally derived core virome, which emerges from comparisons between the viromes of laboratory-reared versus natural mosquito populations. The viromes of natural populations often prove to be richer, due to a greater proportion of environmentally derived viral taxa, and more diverse, as environmentally derived members may be habitat-specific and may naturally fluctuate with time, climate, and other ecological factors [7,9]. It follows that an environmentally derived core virome would be less stable than a vertically transmitted one and must be interpreted within defined spatial and temporal scales. ...

... Overuse of pyrethroids for mosquito control, as well as environmental exposure from other sources (e.g., urban runoff, agriculture, pest control), can result in strong selection pressure for resistant individuals within populations. Pyrethroid resistance in Floridian Ae. aegypti populations has been well documented (Estep et al. 2018, Parker et al. 2020, Schluep and Buckner 2021, Scott et al. 2021. In particular, Parker et al. (2020) recently tested 37 Ae. ...

... Additionally, we included 7 Ae. mascarensis samples from Madagascar (n = 4) and Mauritius (n = 3) as outgroup [37][38][39][40][41] (Table S1). ...

... DNA was extracted using a magnetic bead-based protocol optimized for mosquito DNA for Next-generation sequencing. 10 The head and thorax were dissected from the sample and hydrated in nuclease-free water for 1 hour at 4°C. Tissues were then removed from the water and homogenised in a mixture of 2 μL Proteinase K (100 mg/mL) and 98 μL PK Buffer in a 1.5 mL Eppendorf microcentrifuge tube (add company name), followed by incubation at 56°C for 2 hours. ...

... One option to overcome this challenge that helps to identify the greatest microbial diversity as possible is to Communicated by Ran Wang. investigate the mosquitoes' microbiomes through metagenomic approaches (Boyles et al. 2020;Brinkmann et al. 2016;Gardy and Loman 2018). ...