Emilie Jardé's research while affiliated with French National Centre for Scientific Research and other places

Publications (101)

Article
Environmental context Organic matter (OM) biodegradation plays a key role as it is one of the main processes causing changes in the amount, composition and properties of OM in sediment. However, a complete understanding of its processes and mechanisms is still not reached. In this study, we aim to explore the chemical composition changes during bio...
Article
Full-text available
The origin of sedimentary organic matter (SOM) has often been mentioned as a driver of SOM reactivity. This was quantified by statistically relating the isotopic and lipid composition of SOM to benthic nutrient fluxes in 200 intertidal mudflats sampled along the Brittany coast (France). The origin of SOM explained 24% and 31% of the variance of NH4...
Preprint
Full-text available
In coastal areas and estuaries, such as those encountered in the western part of France (Brittany region), the recycling of carbon and nutrients from sediments can participate in the development of micro and macro-algal blooms with harmful consequences for these ecosystems. One of the main processes controlling this recycling is the microbial miner...
Article
Full-text available
A broad sampling program was carried out in the spring of 2019 on the Brittany coast to assess how the surface sediment characteristics drive the benthic effluxes of ammonium (NH + 4) and phosphate (PO 4) from intertidal mudflats. A total of 200 sediment samples were characterized by their porosity, grain-size, elemental composition and pigment con...
Article
Full-text available
In tropical montane South-East Asia, recent changes in land use have induced increased runoff, soil erosion and in-stream suspended sediment loads. Land use change is also contributing to increased microbial pathogen dissemination and contamination of stream waters. Escherichia coli (E. coli) is frequently used as an indicator of faecal contaminati...
Article
In intensive livestock areas, veterinary pharmaceutical residues (VPRs) can occur in water resources, but also in tap water because treatment processes are not designed to remove these contaminants. The main objective of this study is to assess the occurrence of VPRs in water resources and tap waters in Brittany. As several identical compounds are...
Article
Tropical montane areas of Southeast Asia are exposed to high-intensity rainfall during the monsoon period. This is particularly problematic in areas where soils on steep slopes are cultivated as it can lead to heavy runoff, high soil erosion, and water pollution. The objective of this paper is to analyse the effect of the impact of raindrops on the...
Article
Full-text available
Identifying the presence of animals based on faecal deposits in modern and ancient environments is of primary importance to archaeologists, ecologists, forensic scientists, and watershed managers, but it has proven difficult to distinguish faecal material to the species level. Until now, four 5β-stanols have been deployed as faecal biomarkers to di...
Data
Sample information and 5β-stanol distribution. Information relative to sample collection and analytical method as presented in Materials and Methods, 5β-stanol distribution and sum, concentration of main 5α-stanols and recovery efficiency of internal standard when added. (XLSX)
Data
5β-stanol names and GC-MS properties. 5β-stanols considered in this study and common 5α-stanols and sterols found in samples analyzed. Chromatographic and mass-spectrometric properties for identification and quantification of listed compounds are also presented. (XLSX)
Data
Characteristics of the first four principal components, variables and species distinction of the PCA built on the distribution of 5β-stanols in all ten species faeces. Characteristics of the HCPC model built on the PCA. Variables and species loadings on PCs 1, 2, 3 and 4 show which variables contribute the most (absolute value) to distinguish betwe...
Data
Characteristics of the first four principal components, variables and species distinction of the PCA built on the distribution of 5β-stanols in human, dog, horse and reindeer faeces (selected for the Tofa site context, Sai͡an Mountains). Characteristics of the HCPC model built on the PCA. Variables and species loadings on PCs 1, 2, 3 and 4 show whi...
Data
Characteristics of the first four principal components, variables and species distinction of the PCA built on the distribution of 5β-stanols in human, dog, horse and reindeer faeces (selected for the I͡Amal site context). Characteristics of the HCPC model built on the PCA.Variables and species loadings on PCs 1, 2, 3 and 4 show which variables cont...
Data
Example GC-MS chromatogram (total ion current) from a reindeer sample (R12, see S2 Table). Retention times correspond to the analytical method used for this sample as described in Materials and Methods and S2 Table. Trivial names of the eleven 5β-stanols considered in this study are labelled black (S1 Table). Trivial names of 5α-stanols and sterol...
Data
Characteristics of the first four principal components, variables and species distinction of the PCA built on the distribution of the four main 5β-stanols (coprostanol, epicoprostanol, 24-ethylcoprostanol and 24-ethylepicoprostanol) 5β-stanols in human, dog, horse and reindeer faeces (selected for the I͡Amal site context). Characteristics of the HC...
Data
Mass spectra and summarised fragmentation patterns of TMSi ether derivatives of eleven 5β-stanols found in faecal samples. M+ = molecular fragment. SC = side chain. TMSiOH = trimethylsilanol fragment. Me = methyl. Identification of 5β-lichestanol was made by comparison with the mass spectra of the TMSi ether derivative of stellasterol (24-methyl-5α...
Data
Characteristics of the first four principal components, variables and species distinction of the PCA built on the distribution of the four main 5β-stanols (coprostanol, epicoprostanol, 24-ethylcoprostanol and 24-ethylepicoprostanol) in human, dog, horse and reindeer faeces (selected for the Tofa site context, Sai͡an Mountains). Characteristics of t...
Data
AMS dating information for the buried soils at the I͡Arte 6 site, I͡Amal peninsula. Red text indicates outliers. The radiocarbon calibration multiplot provides a graphical summary of the data in the S2 Table. The results show that the buried soils adjacent to I͡Arte 6, which contained faecal lipids, developed between the 6th and early 11th century...
Data
Comparison of diet and species identification between ratios and multivariate analyses for both case study sites. (XLSX)
Article
Veterinary pharmaceuticals (VPs) increasingly used in animal husbandry have led to their presence in aquatic environments -surface water (SW) or groundwater (GW) - and even in tap water. This review focuses on studies from 2007 to 2017. Sixty-eight different veterinary pharmaceutical residues (VPRs) have been quantified worldwide in natural waters...
Article
The evolution of rare earth element (REE) speciation between reducing and oxidizing conditions in a riparian wetland soil was studied relative to the size fractionation of the solution. In all size fractions obtained from the reduced and oxidized soil solutions, the following analyses were carried out: organic matter (OM) characterization, transmis...
Article
This study identified sources of fecal contamination in three different French headwater and coastal catchments (the Justiçou, Pen an Traon, and La Fresnaye) using a combination of microbial source tracking tools. The tools included bacterial markers (three host-associated Bacteroidales) and chemical markers (six fecal stanols), which were monitore...
Article
Veterinary pharmaceuticals, widely used in intensive livestock production, may contaminate surface waters. Identifying their sources and pathways in watersheds is difficult because i) most veterinary pharmaceuticals are used in human medicine as well and ii) septic or sewer wastewater treatment plants (WWTP) can release pharmaceuticals into surface...
Article
Plastics can be found in all ecosystems across the globe. This type of environmental pollution is important, even if its impact is not fully understood. The presence of small plastic particles at the micro- and nanoscales is of growing concern, but nanoplastic has not yet been observed in natural samples. In this study, we examined four size fracti...
Article
Although the behavior of Arsenic (As) under reducing conditions in periods of high water levels in wetlands is well understood and documented, there is a lack of information under oxidizing conditions when the water level decreases. In this study, we were interested in the first stage of the oxidizing period, when oxidation products are still in su...
Article
Contamination of coastal and shellfish harvesting areas from human and animal waste poses sanitary risks and leads to economic losses. Mussels, oysters (mainly Crassostrea gigas), and similar suspension filter-feeders are particularly sensitive to microbial pathogens, because they accumulate environmental contaminants in their tissues.
Article
In this study, the capacity of oysters to bioaccumulate fecal stanols and to record a source-specific fingerprint was investigated by the short-term contamination of seawater microcosms containing oysters with a human effluent. Contaminated oysters bioaccumulated the typical fecal stanols coprostanol and 24-ethylcoprostanol and their bioaccumulatio...
Article
The objective of this work was to study the effects of washing and purification steps on qualitative and quantitative analysis of fecal stanols in the oyster Crassostrea gigas using either single or a combination of lipid purification steps on silica gel or aminopropyl bonded silica gel (NH2) or a washing step. Among the three analytical pathways c...
Technical Report
Full-text available
Afin d’évaluer les risques pour les écosystèmes ou pour la santé publique liés à la présence de résidus pharmaceutiques (RP) dans les eaux naturelles, il est nécessaire de quantifier une grande diversité de produits à des concentrations parfois très faibles (ng.L-1). Les méthodes analytiques pour la quantification de ces RP sont aujourd’hui très dé...
Article
Runoff of cattle manures (cows, pigs, sheeps) or discharge of effluent from wastewater treatment plants (WWTP) into aquatic ecosystems can lead to microbiological contamination of waters and living organisms. In coastal ecosystems and particularly in shellfish harvesting areas, the presence of pathogen microorganisms in waters induces fecal contami...
Article
Improving the microbiological quality of coastal and river waters relies on the development of reliable markers that are capable of determining sources of fecal pollution. Recently, a principal component analysis (PCA) method based on six stanol compounds (i.e. 5β-cholestan-3β-ol (coprostanol), 5β-cholestan-3α-ol (epicoprostanol), 24-methyl-5α-chol...
Article
Fecal contaminations of inland and coastal waters induce risks to human health and economic losses. To improve water management, specific markers have been developed to differentiate between sources of contamination. This study investigates the relative decay of fecal indicator bacteria (FIB, Escherichia coli and enterococci) and six human-associat...
Article
- Dans le contexte de la Directive 2006/7/CE qui demande de constituer des profils de baignade nécessitant une identification et une hiérarchisation des sources de pollutions fécales, six laboratoires de recherche français se sont associés pour développer une boîte à outils constituée de marqueurs chimiques et microbiologiques destinée à déterminer...
Article
Full-text available
La pollution organique issue des effluents d'élevage et des stations d'épuration urbaines conduit à un problème essentiel de santé publique lié à la contamination des eaux de surface où s'exercent des activités sensibles telles que la baignade. S'il est possible de déterminer les pollutions localisées liées à un dysfonctionnement des systèmes de tr...
Article
In the context of the 2006/7/EC directive requiring to establish a profile for each bathing water, with the identification and hierarchization of faecal pollution sources, six French research laboratories have worked together to develop a Microbial Source Tracking (MST) toolbox including microbial and chemical markers to determine the human and ani...
Conference Paper
Fecal contaminations of inland and coastal waters induce risks to human health and economic losses. In order to improve water management, it is necessary to identify the sources of contamination, which implies the development of specific markers. In order to be considered as a valuable host-specific marker, one must (1) be source specific, (2) occu...
Article
Natural seawater and freshwater microcosms inoculated with pig manure were set up to determine the persistence of pig faecal microbial and chemical markers in these two types of surface water. The concentrations of Lactobacillus amylovorus, the Bacteroidales Pig-2-Bac 16S rRNA genetic marker, five stanols and the evolution of two ratios of stanols,...
Article
Thirty-five samples of cow feces (cowpat and cow manure) and pig slurries subjected to different treatment processes and different storage times before land spreading were extracted and analyzed by gas chromatography-mass spectrometry to determine their fecal stanol profiles. The fresh pig slurry data presented here increase considerably the classi...
Article
Fecal contamination of water resources is evaluated by the enumeration of the fecal coliforms and Enterococci. However, the enumeration of these indicators does not allow us to differentiate between the sources of fecal contamination. Therefore, it is important to use alternative indicators of fecal contamination to identify livestock contamination...
Article
Faecal sterols have been proposed as direct chemical markers for the determination of faecal contamination in inland and coastal waters. In this study, we assess the impact of (a) the concentration of dissolved organic carbon (DOC), (b) the nature of DOC, (c) the salinity and (d) the concentration of sterols and stanols on their solid phase extract...
Conference Paper
Cattle and pig manure contain pathogenic micro-organisms which can be transferred to soil through land spreading and thence to surface water. Such faecal pollution may pose risks to human health. However, the bacteria currently monitored to assess faecal pollution (E. coli, faecal coliforms and enterococci) do not distinguish between faecal polluti...
Article
The microbiological quality of coastal or river waters can be affected by faecal pollution from human or animal sources. An efficient MST (Microbial Source Tracking) toolbox consisting of several host-specific markers would therefore be valuable for identifying the origin of the faecal pollution in the environment and thus for effective resource ma...
Article
The faecal pollutions contribute to the degradation of aquatic ecosystems, affect water quality and thus generate public health problems. In a context of intensive agriculture, animal faeces or sludge from wastewater are applied to fields in the place of traditional mineral fertilizers. According to the implementation of the Water Framework Directi...
Article
Fecal pollution affects environmental waters through spreading of animal manure and runoff of agricultural soils. However, the indicator bacteria (E. coli and enterococci) do not identify the source of the contamination and the development of fecal source tracking tools is crucial for water management and remediation. The aim of our study was to ev...
Article
Soluble organic fractions from soils of two agricultural sites from Brittany (France) have been analyzed to (i) identify the source of polar compounds in soils and (ii) evaluate the impact of organic fertilization and crop type on the distribution and concentration of polar compounds in soils. The main sources of polar compounds in soils are higher...
Article
Malgré les importants efforts consentis ces dernières années pour limiter la contamination des eaux de surface (mise aux normes des stations d'épuration, des installations agricoles, traitements des effluents d'élevage...), leur qualité est encore insuffisante sur de nombreux sites et non conforme aux normes européennes. Les paramètres actuellement...
Article
In freshwater systems, organic micropollutants are bound to natural organic matter (NOM), which is responsible for a decrease in their recoveries by solid-phase extraction (SPE). This "negative effect" has been investigated for the SPE of polycyclic aromatic hydrocarbons (PAHs), oxygenated PAHs, nitrated PAHs and n-alkanes from salt water using Ald...
Article
It is essential to have suitable tools able to trace the fate of manure organic matter in the environment to assess whether manure disposal on the soils of catchments could affect the organic quality of rivers. Sterol compounds – mainly expressed as C29+28/C27 and 5β/C27 ratios – have been shown to be specific molecular tracers of pig, dairy and po...
Article
Full-text available
Samples of pig slurry, as well as dairy and poultry manures collected in Brittany (western France) were analysed to test the ability of sterol profiles to provide a fingerprint of pig slurry contribution to soil organic matter. The data show that the 5β-stanol, known as coprostanol, is the most abundant sterol present in pig slurry, whereas this co...
Article
This study presents long-term records of dissolved organic matter (DOM) as indicated by oxydability measurements that were constructed for four rivers in western France as back as 1979. Data show contrasted evolutions with three rivers exemplifying large increases in DOM concentrations over the period of study (a doubling of the mean annual concent...
Article
Full-text available
The macromolecular characterization of fossil or recent organic matter in soils and sediments is frequently carried out using flash pyrolysis-gas chromatography—mass spectrometry (PyGC–MS). Such analyses providing information on the organic matter structure and reactivity, can be applied on isolated organic materials or on raw samples. Nevertheless...
Article
The lipidic organic fraction of 48 sewage sludges that originated from food-processing, paper-mill and domestic (urban, small urban, and rural) wastewater-treatment plants of the Lorraine region (Northeast of France) was characterised by gas chromatography coupled with mass spectrometer (GC-MS). This study enables us to define an average organic co...
Article
The solid organic fractions of sewage sludges originating from food-processing and domestic (rural and urban) depuration systems of Lorraine (Northeast of France) were characterized by flash pyrolysis coupled with gas chromatography/atomic emission detector (Py-GC/AED). These analyses generated a high amount of data which could not be processed wit...
Article
The aim of this study is to determine a methodology enabling us to simplify the information generated by thermally assisted hydrolysis and methylation (THM)-pyrograms of sludges and to provide an insight into the sludges according to their chemical composition. The insoluble organic fractions of 48 sewage sludges originating from industrial (food-p...
Article
The distribution of humic substances and lipids extracted from two sewage sludges (S1 and S2) resulting from different wastewater and sludge processes was studied. The humic acids were investigated using elemental analysis, infrared spectroscopy and thermally assisted hydrolysis and methylation coupled to gas chromatography–mass spectrometry (THM-G...
Thesis
Cette étude s'est intéressée à la caractérisation moléculaire du contenu organique de boues résiduaires de stations d'épuration de la région Lorraine. Les quarante huit échantillons collectés proviennent de stations domestiques de différentes tailles (rurales, petites urbaines et urbaines), de stations industrielles (papeteries) et de stations agro...
Article
Flash Pyrolysis coupled with Gas Chromatography and Atomic Emission Detection (PyGC–AED) is applied to river sediments and sewage sludges. The chromatograms obtained on such samples are complex and special care must be taken in the tuning of the pyrolyzer, the chromatographic program and the atomic emission detector. Thus, thermodesorption and pyro...
Article
Humic substances were extracted from two sediment cores sampled in a river on the outskirts of a large city (Deûle near Lille, France) and in its highly polluted oxbow-lake. Humic acids (HA) were characterized by infrared spectroscopy and in situ pyrolysis/methylation–gas chromatography–mass spectrometry. The main results are the substantial alipha...

Citations

... The opposite directions of diffusive fluxes between dry and rainy seasons are caused by the seasonal concentrations of nutrients in the overlying water and porewater, altered by physicochemical and biogeochemical properties of the seawater and the sediment (Zhang et al., 2019;Louis et al., 2021). During the rainy season, runoff and sewage discharge promoted riverine nutrient inputs into the bay, which were subsequently accumulated in the sediment. ...
... Furthermore, the widespread use of inadequately composted manure or feces from domestic or wild animals to fertilize and enhance soil structure has contributed to the spread of these microorganisms in the environment [60,61,67,70,12a]. Crops, especially those growing closer to the ground, such as lettuce, are particularly vulnerable, as they may encounter soil during cultivation, irrigation, or heavy rains [69,71,72]. To mitigate the risk of introducing pathogens through organic fertilizer, it is recommended to allow a minimum of 90-120 days to pass (depending on whether the edible portions are in contact with the soil or not) between manure application and plant harvesting. ...
... Fecal steroids, such as sterols and stanols, are promising biomarkers to investigate the source of fecal inputs (Argiriadis et al., 2018;Baeten et al., 2012;Battistel et al., 2017;Harrault et al., 2019;Prost et al., 2017;Reggio et al., 2023;Shah et al., 2007;Shillito et al., 2011;Shillito et al., 2000;Vallejo et al., 2022;Vallejo et al., 2023;Vázquez et al., 2021). Fecal sterols and stanols are chemical compounds widely used for the determination of sewage pollution in water and sediments (Leeming et al., 1996;Leeming et al., 1994;Sánez et al., 2017;He et al., 2018;Isobe et al., 2002;Chan et al., 1998, among others). ...
... Several authors have investigated the occurrence of different classes of PhACs in streams and rivers in various regions of the world. Although most studies focus on molecules for human use, veterinary PhACs and their metabolites have been detected in natural waters worldwide at concentrations ranging from less than one ng L − 1 to several hundred μg L − 1 or more in rare cases (Obimakinde et al., 2017;Charuaud et al., 2019b;Wei et al., 2011;Zhou et al., 2013). Most of these studies have focused on antibiotics. ...
... Surface soil hydraulic properties, including saturated soil hydraulic conductivity (Ks) and sorptivity (S) that have a direct impact on infiltration (Philip, 1957;Parlange et al., 1982;Haverkamp et al., 1994), can change even at very short temporal scales, such as during a rainfall event or between closely spaced rainstorms (Fohrer et al., 1999;Mügler et al., 2019;Ndiaye et al., 2005). The highly dynamic nature of the upper soil layer makes description of changes of structure dependent soil hydraulic properties necessary but very complex (Somaratne and Smettem, 1993;Ndiaye et al., 2005;Todisco et al., 2023). ...
... WWTPs are not always effective in removing these pharmaceuticals, so a high concentration of drugs can be discharged into the environment [10]. This inadequate removal of antibiotics can result in long-term human and animal exposure to their residues, resulting, in addition to AMR, in adverse biological effects [11,12]. As an example, the accumulation of CECs in aquatic organisms can be responsible for their introduction into the human food chain through seafood consumption [13]. ...
... Les 6 ventilateurs (un pour chaque espace de fermentation) sont programmés pour fonctionner par cycles de 30 minutes d'aération/30 minutes d'arrêt. Pour les écorces, la quantité de matière organique contenue dans la phase solide a été estimée à partir de données issues de la littérature (fraction de matières volatiles contenues dans les écorces, La composition de la matière organique contenue dans les boues est complexe (Jardé 2002). En effet, les boues sont constituées de nombreuses molécules (glucides, lipides, protéines, …) ayant chacune sa propre cinétique de biodégradation. ...
... There are many studies demonstrating that the organic-mineral (Fe and Al oxyhydroxides) colloids are the main factor affecting REE transport (Liu et al., 2019b;Lafrenière et al., 2023). It is the result of organic matter encapsulation of minerals because the organic matter stabilizes the mineral colloids through charge and steric repulsion (Pédrot et al., 2015;Guénet et al., 2018;Li et al., 2020). However, the nature of the binding component of REEs in organic-mineral colloids may be difficult to explore. ...
... The world's plastic production continues to increase and with recycling rates lagging, plastic waste continues to accumulate. 15 Approximately 75% of used plastic is either landfilled or cast into the environment, 16 leading to fragmentation into smaller objects, i.e., microplastics (sizes between 1 and 5mm) 17 and nanoplastics (sizes between 1nm and 1 ) [18][19][20][21][22][23][24][25] (MNPLs). The temporal persistence and size of these particles allow them to penetrate cell membranes and accumulate within organisms, potentially leading to harmful effects in aquatic life, 26 plants, 27,28 and humans. ...
... The previous 24 and 72 hours of precipitation in centimeters (cm) was determined by referencing reports gathered by the Community Collaborative Rain, Hail, and Snow Network at site NC-CR-139: Beaufort 0.5 W [26]. Any sampling date with measurable precipitation during both 24 hours and 72 hours prior was deemed a wet weather event ( Table 2). Both precipitation measurements were used as several studies have shown that precipitation three days prior to sample collection has an impact on the concentration of microorganisms [2,27,28]. Wind speed and direction were collected from NOAA's meteorological observation dataset recorded at the Duke Marine Lab, station id: 8656483, in Beaufort, NC [25]. Wind speed was reported in meters per second (m/s) and the wind direction was reported in degrees from true North. ...